Restriction map for
pFN21AE6589
(Circular) (Six-base) MAPSORT of: pFN21AE6589.seq Check: 1375 from: 1 to: 5239
pFN21AE6589 5239 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3049 278
Size: 53 83 186 936 1513 2468
Fragments arranged by size:
2468 1513 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2582 1937
Size: 645 4594
Acc65I G'GTAC_C
Cuts at: 2560 2560
Size: 5239
AclI AA'CG_TT
Cuts at: 1563 3366 3739 1563
Size: 1803 373 3063
Fragments arranged by size:
3063 1803 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3302 4686 4954 1632
Size: 1670 1384 268 1917
Fragments arranged by size:
1917 1670 1384 268
AfeI AGC'GCT
Cuts at: 2372 2372
Size: 5239
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5042
AflIII A'CryG_T
Cuts at: 1203 2200 2424 4138 5115 1203
Size: 997 224 1714 977 1327
Fragments arranged by size:
1714 1327 997 977 224
AgeI A'CCGG_T
Cuts at: 4070 4070
Size: 5239
AhdI GACnn_n'nnGTC
Cuts at: 3968 3968
Size: 5239
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5239
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5239
AlwNI CAG_nnn'CTG
Cuts at: 2982 4554 2982
Size: 1572 3667
ApaLI G'TGCA_C
Cuts at: 2048 3296 4452 2048
Size: 1248 1156 2835
Fragments arranged by size:
2835 1248 1156
ApoI r'AATT_y
Cuts at: 1493 2228 2548 2774 2926 5035 1493
Size: 735 320 226 152 2109 1697
Fragments arranged by size:
2109 1697 735 320 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3793 160
Size: 872 2761 1606
Fragments arranged by size:
2761 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5239
AvaI C'yCGr_G
Cuts at: 1870 1943 2014 2280 2564 1870
Size: 73 71 266 284 4545
Fragments arranged by size:
4545 284 266 73 71
AvrII C'CTAG_G
Cuts at: 2131 2131
Size: 5239
BamHI G'GATC_C
Cuts at: 2569 2936 2569
Size: 367 4872
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2497 2560 4015 618
Size: 325 217 627 710 63 1455 1842
Fragments arranged by size:
1842 1455 710 627 325 217 63
BanII G_rGCy'C
Cuts at: 1391 2350 2558 1391
Size: 959 208 4072
Fragments arranged by size:
4072 959 208
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2259 928
Size: 971 360 3908
Fragments arranged by size:
3908 971 360
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3430 3464 1903
Size: 34 1493 34 3678
Fragments arranged by size:
3678 1493 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2152 3131 4341 2152
Size: 979 1210 3050
Fragments arranged by size:
3050 1210 979
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5239
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3850 136
Size: 107 122 71 631 605 2178 1525
Fragments arranged by size:
2178 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5234 5234
Size: 5239
BlpI GC'TnA_GC
Cuts at: 2656 2656
Size: 5239
Bme1580I G_kGCm'C
Cuts at: 1790 2052 2432 3300 4456 1790
Size: 262 380 868 1156 2573
Fragments arranged by size:
2573 1156 868 380 262
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5239
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2235 2970 3928
Size: 416 53 83 399 24 818 735 958
Cuts at: 3928 442
Size: 1753
Fragments arranged by size:
1753 958 818 735 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5239
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3899 1239
Size: 2660 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3364 4229 4527 3364
Size: 865 298 4076
Fragments arranged by size:
4076 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2121 3902 882
Size: 617 622 1781 2219
Fragments arranged by size:
2219 1781 622 617
BsaAI yAC'GTr
Cuts at: 493 1204 2201 2427 3030 493
Size: 711 997 226 603 2702
Fragments arranged by size:
2702 997 711 603 226
BsaBI GATnn'nnATC
Cuts at: 5233 5233
Size: 5239
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3046 3428 4063 275
Size: 53 83 186 936 1513 382 635 1451
Fragments arranged by size:
1513 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2252 3672 4070 4344 4491 4902 2252
Size: 1420 398 274 147 411 2589
Fragments arranged by size:
2589 1420 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2185 2215 1178
Size: 30 165 30 782 30 4202
Fragments arranged by size:
4202 782 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2139 2495 1186
Size: 953 356 3930
Fragments arranged by size:
3930 953 356
BseYI C'CCAG_C
Cuts at: 2105 4442 4938 2105
Size: 2337 496 2406
Fragments arranged by size:
2406 2337 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2320 1163
Size: 573 584 4082
Fragments arranged by size:
4082 584 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2041 2689 3450 4478 1540
Size: 275 186 40 648 761 1028 2301
Fragments arranged by size:
2301 1028 761 648 275 186 40
BsiHKAI G_wGCw'C
Cuts at: 2052 2290 3300 3385 4456 2052
Size: 238 1010 85 1071 2835
Fragments arranged by size:
2835 1071 1010 238 85
BsmI GAATG_Cn'
Cuts at: 2750 2843 2750
Size: 93 5146
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2290 2350 2432 2558 3300 3385
Size: 399 262 238 60 82 126 742 85
Cuts at: 3385 4456 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 262 238 126 85
82 60
BspEI T'CCGG_A
Cuts at: 2252 4902 2252
Size: 2650 2589
BspHI T'CATG_A
Cuts at: 3128 4858 3128
Size: 1730 3509
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2594 844
Size: 1750 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3126 1394
Size: 429 1303 3507
Fragments arranged by size:
3507 1303 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3734 3908 65
Size: 1183 2486 174 1396
Fragments arranged by size:
2486 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3883 4070 1700
Size: 110 2073 187 2869
Fragments arranged by size:
2869 2073 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5239
BssHII G'CGCG_C
Cuts at: 1925 2030 1925
Size: 105 5134
BssSI C'ACGA_G
Cuts at: 3299 4311 3299
Size: 1012 4227
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5239
BstBI TT'CG_AA
Cuts at: 4043 4043
Size: 5239
BstEII G'GTnAC_C
Cuts at: 1184 2060 1184
Size: 876 4363
BstYI r'GATC_y
Cuts at: 1972 2569 2936 3321 3338 4790 4876 4888 5234
Size: 597 367 385 17 1452 86 12 346
Cuts at: 5234 1972
Size: 1977
Fragments arranged by size:
1977 1452 597 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2331 513
Size: 552 939 327 3421
Fragments arranged by size:
3421 939 552 327
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4500
Fragments arranged by size:
4500 683 56
BtsI GCAGTG_nn'
Cuts at: 2755 3549 3569 2755
Size: 794 20 4425
Fragments arranged by size:
4425 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5239
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5204
DraI TTT'AAA
Cuts at: 2543 2543
Size: 5239
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4246 809
Size: 507 2930 1802
Fragments arranged by size:
2930 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2038 2686 3575
Size: 54 1425 50 275 18 208 648 889
Cuts at: 3575 5204 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 648 275 208 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2038 2686 1537
Size: 275 226 648 4090
Fragments arranged by size:
4090 648 275 226
EarI CTCTTCn'nnn_
Cuts at: 1353 3169 1353
Size: 1816 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2351 3826 4200 4346 387
Size: 1064 138 762 1475 374 146 1280
Fragments arranged by size:
1475 1280 1064 762 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3302 3899 4686 4954 1239
Size: 393 1670 597 787 268 1524
Fragments arranged by size:
1670 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2299 2363 2299
Size: 64 5175
EcoRI G'AATT_C
Cuts at: 2548 2926 2548
Size: 378 4861
FspI TGC'GCA
Cuts at: 3745 3745
Size: 5239
HaeII r_GCGC'y
Cuts at: 1678 2374 2389 4386 1678
Size: 696 15 1997 2531
Fragments arranged by size:
2531 1997 696 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3880 3912 3954 3986 1373
Size: 32 135 32 2308 32 42 32 2626
Fragments arranged by size:
2626 2308 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2583 2829 669
Size: 988 281 645 246 3079
Fragments arranged by size:
3079 988 645 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5239
HpaI GTT'AAC
Cuts at: 2829 2829
Size: 5239
KpnI G_GTAC'C
Cuts at: 2564 2564
Size: 5239
MfeI C'AATT_G
Cuts at: 2838 2838
Size: 5239
MluI A'CGCG_T
Cuts at: 5115 5115
Size: 5239
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2155 4353 4537 1305
Size: 850 2198 184 2007
Fragments arranged by size:
2198 2007 850 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3417
Fragments arranged by size:
3417 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3197 3556 3715 518
Size: 648 2031 359 159 2042
Fragments arranged by size:
2042 2031 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2308 2333 2655 3332 4480 4725 1606
Size: 702 25 322 677 1148 245 2120
Fragments arranged by size:
2120 1148 702 677 322 245 25
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3748
Fragments arranged by size:
3748 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5239
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5239
NotI GC'GGCC_GC
Cuts at: 2686 2686
Size: 5239
NspI r_CATG'y
Cuts at: 1132 1229 2597 4142 1132
Size: 97 1368 1545 2229
Fragments arranged by size:
2229 1545 1368 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5239
PciI A'CATG_T
Cuts at: 4138 4138
Size: 5239
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2112 1108
Size: 1004 4235
PmeI GTTT'AAAC
Cuts at: 2543 2543
Size: 5239
PmlI CAC'GTG
Cuts at: 2201 2427 2201
Size: 226 5013
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3308 3340 4821 4853 3308
Size: 32 1481 32 3694
Fragments arranged by size:
3694 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5239
PsiI TTA'TAA
Cuts at: 2809 2809
Size: 5239
PstI C_TGCA'G
Cuts at: 830 2103 2591 830
Size: 1273 488 3478
Fragments arranged by size:
3478 1273 488
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5239
RsrII CG'GwC_CG
Cuts at: 2043 2043
Size: 5239
SacII CC_GC'GG
Cuts at: 2334 2334
Size: 5239
SalI G'TCGA_C
Cuts at: 1936 2581 1936
Size: 645 4594
SbfI CC_TGCA'GG
Cuts at: 2591 2591
Size: 5239
ScaI AGT'ACT
Cuts at: 3487 5199 3487
Size: 1712 3527
SfcI C'TryA_G
Cuts at: 826 1045 2099 2587 3722 4403 4594 826
Size: 219 1054 488 1135 681 191 1471
Fragments arranged by size:
1471 1135 1054 681 488 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5239
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5239
SmaI CCC'GGG
Cuts at: 1872 2016 2566 1872
Size: 144 550 4545
Fragments arranged by size:
4545 550 144
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3343 4244 4506 820
Size: 197 926 1400 901 262 1553
Fragments arranged by size:
1553 1400 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3030 493
Size: 2537 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5239
SphI G_CATG'C
Cuts at: 1132 2597 1132
Size: 1465 3774
SspI AAT'ATT
Cuts at: 5 52 3163 5
Size: 47 3111 2081
Fragments arranged by size:
3111 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5239
StyI C'CwwG_G
Cuts at: 513 1065 2004 2131 2678 513
Size: 552 939 127 547 3074
Fragments arranged by size:
3074 939 552 547 127
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2427 3266 3283 3436 3621 4066 325
Size: 1255 847 839 17 153 185 445 1498
Fragments arranged by size:
1498 1255 847 839 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3485 5197 96
Size: 274 80 33 51 1443 1508 1712 138
Fragments arranged by size:
1712 1508 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2185 2217 5199 5231 2185
Size: 32 2982 32 2193
Fragments arranged by size:
2982 2193 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5239
XbaI T'CTAG_A
Cuts at: 2575 2575
Size: 5239
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5239
XmaI C'CCGG_G
Cuts at: 1870 2014 2564 1870
Size: 144 550 4545
Fragments arranged by size:
4545 550 144
XmnI GAAnn'nnTTC
Cuts at: 3368 3368
Size: 5239
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3047 276
Size: 53 83 186 936 1513 2468
Fragments arranged by size:
2468 1513 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PstI PvuI RsrII
SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI
PspXI PsrI PvuII SacI SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI