Restriction map for
pFN21AE6554
(Circular) (Six-base) MAPSORT of: pFN21AE6554.seq Check: 1783 from: 1 to: 5161
pFN21AE6554 5161 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2971 278
Size: 53 83 186 936 1435 2468
Fragments arranged by size:
2468 1435 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2504 1937
Size: 567 4594
Acc65I G'GTAC_C
Cuts at: 2482 2482
Size: 5161
AclI AA'CG_TT
Cuts at: 1563 3288 3661 1563
Size: 1725 373 3063
Fragments arranged by size:
3063 1725 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2216 3224 4608 4876 1632
Size: 584 1008 1384 268 1917
Fragments arranged by size:
1917 1384 1008 584 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4964
AflIII A'CryG_T
Cuts at: 1203 2184 4060 5037 1203
Size: 981 1876 977 1327
Fragments arranged by size:
1876 1327 981 977
AgeI A'CCGG_T
Cuts at: 3992 3992
Size: 5161
AhdI GACnn_n'nnGTC
Cuts at: 3890 3890
Size: 5161
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5161
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5161
AlwNI CAG_nnn'CTG
Cuts at: 2904 4476 2904
Size: 1572 3589
ApaI G_GGCC'C
Cuts at: 2109 2219 2109
Size: 110 5051
ApaLI G'TGCA_C
Cuts at: 3218 4374 3218
Size: 1156 4005
ApoI r'AATT_y
Cuts at: 1493 2470 2696 2848 4957 1493
Size: 977 226 152 2109 1697
Fragments arranged by size:
2109 1697 977 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3715 160
Size: 872 2683 1606
Fragments arranged by size:
2683 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5161
AvaI C'yCGr_G
Cuts at: 1870 1943 2011 2486 1870
Size: 73 68 475 4545
Fragments arranged by size:
4545 475 73 68
BamHI G'GATC_C
Cuts at: 2491 2858 2491
Size: 367 4794
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2449 2482 3937 618
Size: 325 217 627 662 33 1455 1842
Fragments arranged by size:
1842 1455 662 627 325 217 33
BanII G_rGCy'C
Cuts at: 1391 2034 2109 2219 2480 1391
Size: 643 75 110 261 4072
Fragments arranged by size:
4072 643 261 110 75
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4190
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3352 3386 1903
Size: 34 1415 34 3678
Fragments arranged by size:
3678 1415 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3053 4263 3053
Size: 1210 3951
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5161
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2081 3772 136
Size: 107 122 71 631 605 409 1691 1525
Fragments arranged by size:
1691 1525 631 605 409 122 107 71
BglII A'GATC_T
Cuts at: 5156 5156
Size: 5161
BlpI GC'TnA_GC
Cuts at: 2578 2578
Size: 5161
Bme1580I G_kGCm'C
Cuts at: 1790 2057 2109 2219 2241 3222 4378 1790
Size: 267 52 110 22 981 1156 2573
Fragments arranged by size:
2573 1156 981 267 110 52 22
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5161
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2892 3850 442
Size: 416 53 83 399 24 1475 958 1753
Fragments arranged by size:
1753 1475 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5161
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2116 3821 1239
Size: 877 1705 2579
Fragments arranged by size:
2579 1705 877
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3286 4151 4449 3286
Size: 865 298 3998
Fragments arranged by size:
3998 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3824 882
Size: 617 2325 2219
Fragments arranged by size:
2325 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2952 493
Size: 711 1748 2702
Fragments arranged by size:
2702 1748 711
BsaBI GATnn'nnATC
Cuts at: 5155 5155
Size: 5161
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2073 2968 3350 3985
Size: 53 83 186 936 540 895 382 635
Cuts at: 3985 275
Size: 1451
Fragments arranged by size:
1451 936 895 635 540 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3594 3992 4266 4413 4824 3594
Size: 398 274 147 411 3931
Fragments arranged by size:
3931 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4936
Fragments arranged by size:
4936 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2036 1186
Size: 850 4311
BseYI C'CCAG_C
Cuts at: 2088 2190 2219 2454 4364 4860 2088
Size: 102 29 235 1910 496 2389
Fragments arranged by size:
2389 1910 496 235 102 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4588
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2611 3372 4400 1540
Size: 275 186 610 761 1028 2301
Fragments arranged by size:
2301 1028 761 610 275 186
BsiHKAI G_wGCw'C
Cuts at: 2034 3222 3307 4378 2034
Size: 1188 85 1071 2817
Fragments arranged by size:
2817 1188 1071 85
BsmI GAATG_Cn'
Cuts at: 2672 2765 2672
Size: 93 5068
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2057 2109 2219 2241 2480 3222
Size: 399 244 23 52 110 22 239 742
Cuts at: 3222 3307 4378 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 244 239 110 85
52 23 22
BspEI T'CCGG_A
Cuts at: 4824 4824
Size: 5161
BspHI T'CATG_A
Cuts at: 3050 4780 3050
Size: 1730 3431
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2449 2516 844
Size: 1605 67 3489
Fragments arranged by size:
3489 1605 67
BsrBI CCG'CTC
Cuts at: 1394 1823 3048 1394
Size: 429 1225 3507
Fragments arranged by size:
3507 1225 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3656 3830 65
Size: 1183 2408 174 1396
Fragments arranged by size:
2408 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2080 3805 3992 1700
Size: 110 270 1725 187 2869
Fragments arranged by size:
2869 1725 270 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5161
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5161
BssSI C'ACGA_G
Cuts at: 3221 4233 3221
Size: 1012 4149
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5161
BstBI TT'CG_AA
Cuts at: 3965 3965
Size: 5161
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5161
BstXI CCAn_nnnn'nTGG
Cuts at: 2377 2377
Size: 5161
BstYI r'GATC_y
Cuts at: 1972 2491 2858 3243 3260 4712 4798 4810 5156
Size: 519 367 385 17 1452 86 12 346
Cuts at: 5156 1972
Size: 1977
Fragments arranged by size:
1977 1452 519 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2370 513
Size: 552 939 366 3304
Fragments arranged by size:
3304 939 552 366
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2149 487
Size: 683 56 923 3499
Fragments arranged by size:
3499 923 683 56
BtsI GCAGTG_nn'
Cuts at: 2677 3471 3491 2677
Size: 794 20 4347
Fragments arranged by size:
4347 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5161
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5126
DraI TTT'AAA
Cuts at: 2465 2465
Size: 5161
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4168 809
Size: 507 2852 1802
Fragments arranged by size:
2852 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2178 2331 2367
Size: 54 1425 50 275 18 348 153 36
Cuts at: 2367 2608 3497 5126 8
Size: 241 889 1629 43
Fragments arranged by size:
1629 1425 889 348 275 241 153 54
50 43 36 18
EagI C'GGCC_G
Cuts at: 1537 1812 2608 1537
Size: 275 796 4090
Fragments arranged by size:
4090 796 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2132 3091 1353
Size: 779 959 3423
Fragments arranged by size:
3423 959 779
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3748 4122 4268 387
Size: 1064 138 2159 374 146 1280
Fragments arranged by size:
2159 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2032 2032
Size: 5161
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2116 2216 3224 3821 4608 4876 1239
Size: 393 484 100 1008 597 787 268 1524
Fragments arranged by size:
1524 1008 787 597 484 393 268 100
EcoO109I rG'GnC_Cy
Cuts at: 2215 2216 2215
Size: 1 5160
EcoRI G'AATT_C
Cuts at: 2470 2848 2470
Size: 378 4783
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2368 2368
Size: 5161
FspI TGC'GCA
Cuts at: 3667 3667
Size: 5161
HaeII r_GCGC'y
Cuts at: 1678 4308 1678
Size: 2630 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3802 3834 3876 3908 1373
Size: 32 135 32 2230 32 42 32 2626
Fragments arranged by size:
2626 2230 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2401 2505 2751 669
Size: 988 281 463 104 246 3079
Fragments arranged by size:
3079 988 463 281 246 104
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5161
HpaI GTT'AAC
Cuts at: 2751 2751
Size: 5161
KpnI G_GTAC'C
Cuts at: 2486 2486
Size: 5161
MfeI C'AATT_G
Cuts at: 2760 2760
Size: 5161
MluI A'CGCG_T
Cuts at: 2184 5037 2184
Size: 2853 2308
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4275 4459 1305
Size: 2970 184 2007
Fragments arranged by size:
2970 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2180 2369 10
Size: 54 1425 343 348 189 2802
Fragments arranged by size:
2802 1425 348 343 189 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2114 2390 3119 3478 3637 518
Size: 648 948 276 729 359 159 2042
Fragments arranged by size:
2042 948 729 648 359 276 159
MspA1I CmG'CkG
Cuts at: 1606 2088 2194 2577 3254 4402 4647 1606
Size: 482 106 383 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 482 383 245 106
NaeI GCC'GGC
Cuts at: 2082 2082
Size: 5161
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3670
Fragments arranged by size:
3670 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5161
NgoMIV G'CCGG_C
Cuts at: 2080 2080
Size: 5161
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5161
NotI GC'GGCC_GC
Cuts at: 2608 2608
Size: 5161
NspI r_CATG'y
Cuts at: 1132 1229 2158 2519 4064 1132
Size: 97 929 361 1545 2229
Fragments arranged by size:
2229 1545 929 361 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5161
PciI A'CATG_T
Cuts at: 4060 4060
Size: 5161
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5161
PmeI GTTT'AAAC
Cuts at: 2465 2465
Size: 5161
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3230 3262 4743 4775 3230
Size: 32 1481 32 3616
Fragments arranged by size:
3616 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5161
PsiI TTA'TAA
Cuts at: 2731 2731
Size: 5161
PspOMI G'GGCC_C
Cuts at: 2105 2215 2105
Size: 110 5051
PstI C_TGCA'G
Cuts at: 830 2277 2513 830
Size: 1447 236 3478
Fragments arranged by size:
3478 1447 236
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5161
PvuII CAG'CTG
Cuts at: 2194 2194
Size: 5161
SacI G_AGCT'C
Cuts at: 2034 2034
Size: 5161
SalI G'TCGA_C
Cuts at: 1936 2503 1936
Size: 567 4594
SapI GCTCTTCn'nnn_
Cuts at: 2132 2132
Size: 5161
SbfI CC_TGCA'GG
Cuts at: 2513 2513
Size: 5161
ScaI AGT'ACT
Cuts at: 3409 5121 3409
Size: 1712 3449
SfcI C'TryA_G
Cuts at: 826 1045 2273 2509 3644 4325 4516 826
Size: 219 1228 236 1135 681 191 1471
Fragments arranged by size:
1471 1228 1135 681 236 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5161
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5161
SmaI CCC'GGG
Cuts at: 1872 2488 1872
Size: 616 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3265 4166 4428 820
Size: 197 926 1322 901 262 1553
Fragments arranged by size:
1553 1322 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2952 493
Size: 2459 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5161
SphI G_CATG'C
Cuts at: 1132 2158 2519 1132
Size: 1026 361 3774
Fragments arranged by size:
3774 1026 361
SspI AAT'ATT
Cuts at: 5 52 3085 5
Size: 47 3033 2081
Fragments arranged by size:
3033 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5161
StyI C'CwwG_G
Cuts at: 513 1065 2004 2600 513
Size: 552 939 596 3074
Fragments arranged by size:
3074 939 596 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3188 3205 3358 3543 3988 325
Size: 1255 1608 17 153 185 445 1498
Fragments arranged by size:
1608 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3407 5119 96
Size: 274 80 33 51 1443 1430 1712 138
Fragments arranged by size:
1712 1443 1430 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5121 5153 5121
Size: 32 5129
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5161
XbaI T'CTAG_A
Cuts at: 2497 2497
Size: 5161
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5161
XmaI C'CCGG_G
Cuts at: 1870 2486 1870
Size: 616 4545
XmnI GAAnn'nnTTC
Cuts at: 3290 3290
Size: 5161
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2969 276
Size: 53 83 186 936 1435 2468
Fragments arranged by size:
2468 1435 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI