Restriction map for
pFN21AE6550
(Circular) (Six-base) MAPSORT of: pFN21AE6550.seq Check: 3780 from: 1 to: 5071
pFN21AE6550 5071 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2881 278
Size: 53 83 186 936 1345 2468
Fragments arranged by size:
2468 1345 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2414 1937
Size: 477 4594
Acc65I G'GTAC_C
Cuts at: 2392 2392
Size: 5071
AclI AA'CG_TT
Cuts at: 1563 3198 3571 1563
Size: 1635 373 3063
Fragments arranged by size:
3063 1635 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3134 4518 4786 1632
Size: 1502 1384 268 1917
Fragments arranged by size:
1917 1502 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4874
AflIII A'CryG_T
Cuts at: 1203 3970 4947 1203
Size: 2767 977 1327
Fragments arranged by size:
2767 1327 977
AgeI A'CCGG_T
Cuts at: 3902 3902
Size: 5071
AhdI GACnn_n'nnGTC
Cuts at: 3800 3800
Size: 5071
AleI CACnn'nnGTG
Cuts at: 1166 2147 1166
Size: 981 4090
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2378 1184
Size: 1194 3877
AlwNI CAG_nnn'CTG
Cuts at: 2814 4386 2814
Size: 1572 3499
ApaLI G'TGCA_C
Cuts at: 3128 4284 3128
Size: 1156 3915
ApoI r'AATT_y
Cuts at: 1493 2380 2606 2758 4867 1493
Size: 887 226 152 2109 1697
Fragments arranged by size:
2109 1697 887 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3625 160
Size: 872 2593 1606
Fragments arranged by size:
2593 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5071
AvaI C'yCGr_G
Cuts at: 1870 1943 2337 2396 1870
Size: 73 394 59 4545
Fragments arranged by size:
4545 394 73 59
BamHI G'GATC_C
Cuts at: 2401 2768 2401
Size: 367 4704
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2392 3847 618
Size: 325 217 627 605 1455 1842
Fragments arranged by size:
1842 1455 627 605 325 217
BanII G_rGCy'C
Cuts at: 1391 2141 2390 1391
Size: 750 249 4072
Fragments arranged by size:
4072 750 249
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4100
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3262 3296 1903
Size: 34 50 34 1241 34 3678
Fragments arranged by size:
3678 1241 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2963 4173 2963
Size: 1210 3861
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5071
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3682 136
Size: 107 122 71 631 605 2010 1525
Fragments arranged by size:
2010 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5066 5066
Size: 5071
BlpI GC'TnA_GC
Cuts at: 2488 2488
Size: 5071
Bme1580I G_kGCm'C
Cuts at: 1790 2261 3132 4288 1790
Size: 471 871 1156 2573
Fragments arranged by size:
2573 1156 871 471
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5071
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2802 3760 442
Size: 416 53 83 399 24 1385 958 1753
Fragments arranged by size:
1753 1385 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5071
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2198 3731 1239
Size: 959 1533 2579
Fragments arranged by size:
2579 1533 959
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3196 4061 4359 3196
Size: 865 298 3908
Fragments arranged by size:
3908 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3734 882
Size: 617 2235 2219
Fragments arranged by size:
2235 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2862 493
Size: 711 1658 2702
Fragments arranged by size:
2702 1658 711
BsaBI GATnn'nnATC
Cuts at: 5065 5065
Size: 5071
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2301 2878 3260 3895
Size: 53 83 186 936 768 577 382 635
Cuts at: 3895 275
Size: 1451
Fragments arranged by size:
1451 936 768 635 577 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3504 3902 4176 4323 4734 3504
Size: 398 274 147 411 3841
Fragments arranged by size:
3841 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4846
Fragments arranged by size:
4846 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2048 1186
Size: 862 4209
BseYI C'CCAG_C
Cuts at: 4274 4770 4274
Size: 496 4575
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4498
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2521 3282 4310 1540
Size: 275 186 520 761 1028 2301
Fragments arranged by size:
2301 1028 761 520 275 186
BsiHKAI G_wGCw'C
Cuts at: 3132 3217 4288 3132
Size: 85 1071 3915
Fragments arranged by size:
3915 1071 85
BsmI GAATG_Cn'
Cuts at: 2582 2675 2582
Size: 93 4978
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2141 2261 2390 3132 3217 4288 1391
Size: 399 351 120 129 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 351 129 120 85
BspEI T'CCGG_A
Cuts at: 4734 4734
Size: 5071
BspHI T'CATG_A
Cuts at: 2287 2960 4690 2287
Size: 673 1730 2668
Fragments arranged by size:
2668 1730 673
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2426 844
Size: 1582 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2958 1394
Size: 429 1135 3507
Fragments arranged by size:
3507 1135 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2069 3566 3740 65
Size: 1183 821 1497 174 1396
Fragments arranged by size:
1497 1396 1183 821 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2240 3715 3902 1700
Size: 110 430 1475 187 2869
Fragments arranged by size:
2869 1475 430 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5071
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5071
BssSI C'ACGA_G
Cuts at: 3131 4143 3131
Size: 1012 4059
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5071
BstBI TT'CG_AA
Cuts at: 3875 3875
Size: 5071
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5071
BstXI CCAn_nnnn'nTGG
Cuts at: 2149 2149
Size: 5071
BstYI r'GATC_y
Cuts at: 1972 2401 2768 3153 3170 4622 4708 4720 5066
Size: 429 367 385 17 1452 86 12 346
Cuts at: 5066 1972
Size: 1977
Fragments arranged by size:
1977 1452 429 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2060 2060
Size: 5071
BtgI C'CryG_G
Cuts at: 513 1065 2013 2160 513
Size: 552 948 147 3424
Fragments arranged by size:
3424 948 552 147
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4332
Fragments arranged by size:
4332 683 56
BtsI GCAGTG_nn'
Cuts at: 2094 2117 2213 2587 3381 3401 2094
Size: 23 96 374 794 20 3764
Fragments arranged by size:
3764 794 374 96 23 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5071
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5036
DraI TTT'AAA
Cuts at: 2375 2375
Size: 5071
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4078 809
Size: 507 2762 1802
Fragments arranged by size:
2762 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2210 2277 2518
Size: 54 1425 50 275 18 380 67 241
Cuts at: 2518 3407 5036 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 380 275 241 67 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2518 1537
Size: 275 706 4090
Fragments arranged by size:
4090 706 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3001 1353
Size: 1648 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2227 3658 4032 4178 387
Size: 1064 138 638 1431 374 146 1280
Fragments arranged by size:
1431 1280 1064 638 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2198 3134 3731 4518 4786 1239
Size: 393 566 936 597 787 268 1524
Fragments arranged by size:
1524 936 787 597 566 393 268
EcoRI G'AATT_C
Cuts at: 2380 2758 2380
Size: 378 4693
FspI TGC'GCA
Cuts at: 3577 3577
Size: 5071
HaeII r_GCGC'y
Cuts at: 1678 4218 1678
Size: 2540 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2115 2147 3712 3744 3786
Size: 32 135 32 543 32 1565 32 42
Cuts at: 3786 3818 1373
Size: 32 2626
Fragments arranged by size:
2626 1565 543 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2415 2661 669
Size: 988 281 477 246 3079
Fragments arranged by size:
3079 988 477 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5071
HpaI GTT'AAC
Cuts at: 2661 2661
Size: 5071
KpnI G_GTAC'C
Cuts at: 2396 2396
Size: 5071
MfeI C'AATT_G
Cuts at: 2670 2670
Size: 5071
MluI A'CGCG_T
Cuts at: 4947 4947
Size: 5071
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4185 4369 1305
Size: 2880 184 2007
Fragments arranged by size:
2880 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2212 2279 10
Size: 54 1425 343 380 67 2802
Fragments arranged by size:
2802 1425 380 343 67 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2147 3029 3388 3547 518
Size: 648 981 882 359 159 2042
Fragments arranged by size:
2042 981 882 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2487 3164 4312 4557 1606
Size: 881 677 1148 245 2120
Fragments arranged by size:
2120 1148 881 677 245
NaeI GCC'GGC
Cuts at: 2242 2242
Size: 5071
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4519
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5071
NgoMIV G'CCGG_C
Cuts at: 2240 2240
Size: 5071
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5071
NotI GC'GGCC_GC
Cuts at: 2518 2518
Size: 5071
NspI r_CATG'y
Cuts at: 1132 1229 2429 3974 1132
Size: 97 1200 1545 2229
Fragments arranged by size:
2229 1545 1200 97
PasI CC'CwG_GG
Cuts at: 1348 2254 1348
Size: 906 4165
PciI A'CATG_T
Cuts at: 3970 3970
Size: 5071
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5071
PmeI GTTT'AAAC
Cuts at: 2375 2375
Size: 5071
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3140 3172 4653 4685 3140
Size: 32 1481 32 3526
Fragments arranged by size:
3526 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5071
PsiI TTA'TAA
Cuts at: 2641 2641
Size: 5071
PstI C_TGCA'G
Cuts at: 830 2319 2423 830
Size: 1489 104 3478
Fragments arranged by size:
3478 1489 104
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5071
SalI G'TCGA_C
Cuts at: 1936 2413 1936
Size: 477 4594
SbfI CC_TGCA'GG
Cuts at: 2423 2423
Size: 5071
ScaI AGT'ACT
Cuts at: 3319 5031 3319
Size: 1712 3359
SfcI C'TryA_G
Cuts at: 826 1045 2315 2419 3554 4235 4426 826
Size: 219 1270 104 1135 681 191 1471
Fragments arranged by size:
1471 1270 1135 681 219 191 104
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5071
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5071
SmaI CCC'GGG
Cuts at: 1872 2398 1872
Size: 526 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3175 4076 4338 820
Size: 197 926 1232 901 262 1553
Fragments arranged by size:
1553 1232 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2862 493
Size: 2369 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5071
SphI G_CATG'C
Cuts at: 1132 2429 1132
Size: 1297 3774
SspI AAT'ATT
Cuts at: 5 52 2995 5
Size: 47 2943 2081
Fragments arranged by size:
2943 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5071
StyI C'CwwG_G
Cuts at: 513 1065 2169 2510 513
Size: 552 1104 341 3074
Fragments arranged by size:
3074 1104 552 341
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2137 3098 3115 3268 3453 3898 325
Size: 1255 557 961 17 153 185 445 1498
Fragments arranged by size:
1498 1255 961 557 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3317 5029 96
Size: 274 80 33 51 1443 1340 1712 138
Fragments arranged by size:
1712 1443 1340 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2192 2224 5031 5063 2192
Size: 32 2807 32 2200
Fragments arranged by size:
2807 2200 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5071
XbaI T'CTAG_A
Cuts at: 2407 2407
Size: 5071
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2167 2011
Size: 156 4915
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5071
XmaI C'CCGG_G
Cuts at: 1870 2396 1870
Size: 526 4545
XmnI GAAnn'nnTTC
Cuts at: 3200 3200
Size: 5071
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2879 276
Size: 53 83 186 936 1345 2468
Fragments arranged by size:
2468 1345 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI