Restriction map for
pFN21AE6537
(Circular) (Six-base) MAPSORT of: pFN21AE6537.seq Check: 3966 from: 1 to: 4939
pFN21AE6537 4939 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2749 278
Size: 53 83 186 936 1213 2468
Fragments arranged by size:
2468 1213 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2282 1937
Size: 345 4594
Acc65I G'GTAC_C
Cuts at: 2260 2260
Size: 4939
AclI AA'CG_TT
Cuts at: 1563 3066 3439 1563
Size: 1503 373 3063
Fragments arranged by size:
3063 1503 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3002 4386 4654 1632
Size: 1370 1384 268 1917
Fragments arranged by size:
1917 1384 1370 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4742
AflIII A'CryG_T
Cuts at: 1203 3838 4815 1203
Size: 2635 977 1327
Fragments arranged by size:
2635 1327 977
AgeI A'CCGG_T
Cuts at: 3770 3770
Size: 4939
AhdI GACnn_n'nnGTC
Cuts at: 3668 3668
Size: 4939
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4939
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4939
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2015 2047 2015
Size: 32 4907
AlwNI CAG_nnn'CTG
Cuts at: 2682 4254 2682
Size: 1572 3367
ApaI G_GGCC'C
Cuts at: 2171 2177 2171
Size: 6 4933
ApaLI G'TGCA_C
Cuts at: 2996 4152 2996
Size: 1156 3783
ApoI r'AATT_y
Cuts at: 1493 2248 2474 2626 4735 1493
Size: 755 226 152 2109 1697
Fragments arranged by size:
2109 1697 755 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3493 160
Size: 872 2461 1606
Fragments arranged by size:
2461 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4939
AvaI C'yCGr_G
Cuts at: 1870 1943 2170 2205 2264 1870
Size: 73 227 35 59 4545
Fragments arranged by size:
4545 227 73 59 35
BamHI G'GATC_C
Cuts at: 2269 2636 2269
Size: 367 4572
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2078 2260 3715 618
Size: 325 217 627 291 182 1455 1842
Fragments arranged by size:
1842 1455 627 325 291 217 182
BanII G_rGCy'C
Cuts at: 1391 2171 2177 2258 1391
Size: 780 6 81 4072
Fragments arranged by size:
4072 780 81 6
BbeI G_GCGC'C
Cuts at: 2082 2082
Size: 4939
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 3968
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3130 3164 1903
Size: 34 1193 34 3678
Fragments arranged by size:
3678 1193 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2831 4041 2831
Size: 1210 3729
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4939
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2087 2166 3550
Size: 107 122 71 631 605 415 79 1384
Cuts at: 3550 136
Size: 1525
Fragments arranged by size:
1525 1384 631 605 415 122 107 79
71
BglII A'GATC_T
Cuts at: 4934 4934
Size: 4939
BlpI GC'TnA_GC
Cuts at: 2356 2356
Size: 4939
Bme1580I G_kGCm'C
Cuts at: 1790 2171 2177 3000 4156 1790
Size: 381 6 823 1156 2573
Fragments arranged by size:
2573 1156 823 381 6
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4939
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2670 3628 442
Size: 416 53 83 399 24 1253 958 1753
Fragments arranged by size:
1753 1253 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4939
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3599 1239
Size: 2360 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3064 3929 4227 3064
Size: 865 298 3776
Fragments arranged by size:
3776 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3602 882
Size: 617 2103 2219
Fragments arranged by size:
2219 2103 617
BsaAI yAC'GTr
Cuts at: 493 1204 2730 493
Size: 711 1526 2702
Fragments arranged by size:
2702 1526 711
BsaBI GATnn'nnATC
Cuts at: 4933 4933
Size: 4939
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2079 2746 3128 3763
Size: 53 83 186 936 546 667 382 635
Cuts at: 3763 275
Size: 1451
Fragments arranged by size:
1451 936 667 635 546 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3372 3770 4044 4191 4602 3372
Size: 398 274 147 411 3709
Fragments arranged by size:
3709 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2015 2045 2184 2214 1178
Size: 30 165 30 612 30 139 30 3903
Fragments arranged by size:
3903 612 165 139 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 4939
BseYI C'CCAG_C
Cuts at: 2195 2228 4142 4638 2195
Size: 33 1914 496 2496
Fragments arranged by size:
2496 1914 496 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1999 1163
Size: 573 263 4103
Fragments arranged by size:
4103 573 263
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2091 2161 2389 3150 4178 1540
Size: 275 186 90 70 228 761 1028 2301
Fragments arranged by size:
2301 1028 761 275 228 186 90 70
BsiHKAI G_wGCw'C
Cuts at: 3000 3085 4156 3000
Size: 85 1071 3783
Fragments arranged by size:
3783 1071 85
BsmI GAATG_Cn'
Cuts at: 2450 2543 2450
Size: 93 4846
BsmBI CGTCTCn'nnnn_
Cuts at: 2229 2229
Size: 4939
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2171 2177 2258 3000 3085 4156 1391
Size: 399 381 6 81 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 381 85 81 6
BspEI T'CCGG_A
Cuts at: 4602 4602
Size: 4939
BspHI T'CATG_A
Cuts at: 2828 4558 2828
Size: 1730 3209
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2294 844
Size: 1450 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2148 2826 1394
Size: 429 325 678 3507
Fragments arranged by size:
3507 678 429 325
BsrDI GCAATG_nn'
Cuts at: 65 1248 3434 3608 65
Size: 1183 2186 174 1396
Fragments arranged by size:
2186 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3583 3770 1700
Size: 110 1773 187 2869
Fragments arranged by size:
2869 1773 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4939
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4939
BssSI C'ACGA_G
Cuts at: 2999 4011 2999
Size: 1012 3927
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4939
BstBI TT'CG_AA
Cuts at: 3743 3743
Size: 4939
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4939
BstYI r'GATC_y
Cuts at: 1972 2269 2636 3021 3038 4490 4576 4588 4934
Size: 297 367 385 17 1452 86 12 346
Cuts at: 4934 1972
Size: 1977
Fragments arranged by size:
1977 1452 385 367 346 297 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3448
Fragments arranged by size:
3448 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4200
Fragments arranged by size:
4200 683 56
BtsI GCAGTG_nn'
Cuts at: 2455 3249 3269 2455
Size: 794 20 4125
Fragments arranged by size:
4125 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4939
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4904
DraI TTT'AAA
Cuts at: 2243 2243
Size: 4939
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3946 809
Size: 507 2630 1802
Fragments arranged by size:
2630 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2088 2158 2386
Size: 54 1425 50 275 18 258 70 228
Cuts at: 2386 3275 4904 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 275 258 228 70 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2088 2158 2386 1537
Size: 275 276 70 228 4090
Fragments arranged by size:
4090 276 275 228 70
EarI CTCTTCn'nnn_
Cuts at: 1353 2869 1353
Size: 1516 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2205 3526 3900 4046 387
Size: 1064 138 616 1321 374 146 1280
Fragments arranged by size:
1321 1280 1064 616 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3002 3599 4386 4654 1239
Size: 393 1370 597 787 268 1524
Fragments arranged by size:
1524 1370 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2167 2174 2183 2167
Size: 7 9 4923
Fragments arranged by size:
4923 9 7
EcoRI G'AATT_C
Cuts at: 2248 2626 2248
Size: 378 4561
FspI TGC'GCA
Cuts at: 3445 3445
Size: 4939
HaeII r_GCGC'y
Cuts at: 1678 2082 4086 1678
Size: 404 2004 2531
Fragments arranged by size:
2531 2004 404
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3580 3612 3654 3686 1373
Size: 32 135 32 2008 32 42 32 2626
Fragments arranged by size:
2626 2008 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2283 2529 669
Size: 988 281 345 246 3079
Fragments arranged by size:
3079 988 345 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4939
HpaI GTT'AAC
Cuts at: 2529 2529
Size: 4939
KasI G'GCGC_C
Cuts at: 2078 2078
Size: 4939
KpnI G_GTAC'C
Cuts at: 2264 2264
Size: 4939
MfeI C'AATT_G
Cuts at: 2538 2538
Size: 4939
MluI A'CGCG_T
Cuts at: 4815 4815
Size: 4939
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4053 4237 1305
Size: 2748 184 2007
Fragments arranged by size:
2748 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3117
Fragments arranged by size:
3117 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2897 3256 3415 518
Size: 648 1731 359 159 2042
Fragments arranged by size:
2042 1731 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2065 2232 2355 3032 4180 4425 1606
Size: 459 167 123 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 459 245 167 123
NarI GG'CG_CC
Cuts at: 2079 2079
Size: 4939
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3448
Fragments arranged by size:
3448 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4939
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4939
NotI GC'GGCC_GC
Cuts at: 2088 2386 2088
Size: 298 4641
NspI r_CATG'y
Cuts at: 1132 1229 2297 3842 1132
Size: 97 1068 1545 2229
Fragments arranged by size:
2229 1545 1068 97
PasI CC'CwG_GG
Cuts at: 1348 2177 1348
Size: 829 4110
PciI A'CATG_T
Cuts at: 3838 3838
Size: 4939
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4939
PmeI GTTT'AAAC
Cuts at: 2243 2243
Size: 4939
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2015 2047 3008 3040 4521 4553 2015
Size: 32 961 32 1481 32 2401
Fragments arranged by size:
2401 1481 961 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4939
PsiI TTA'TAA
Cuts at: 2509 2509
Size: 4939
PspOMI G'GGCC_C
Cuts at: 2167 2173 2167
Size: 6 4933
PstI C_TGCA'G
Cuts at: 830 2291 830
Size: 1461 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4939
SalI G'TCGA_C
Cuts at: 1936 2281 1936
Size: 345 4594
SbfI CC_TGCA'GG
Cuts at: 2291 2291
Size: 4939
ScaI AGT'ACT
Cuts at: 3187 4899 3187
Size: 1712 3227
SfcI C'TryA_G
Cuts at: 826 1045 2287 3422 4103 4294 826
Size: 219 1242 1135 681 191 1471
Fragments arranged by size:
1471 1242 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2166 2166
Size: 4939
SfoI GGC'GCC
Cuts at: 2080 2080
Size: 4939
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4939
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4939
SmaI CCC'GGG
Cuts at: 1872 2172 2207 2266 1872
Size: 300 35 59 4545
Fragments arranged by size:
4545 300 59 35
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3043 3944 4206 820
Size: 197 926 1100 901 262 1553
Fragments arranged by size:
1553 1100 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2730 493
Size: 2237 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4939
SphI G_CATG'C
Cuts at: 1132 2297 1132
Size: 1165 3774
SrfI GCCC'GGGC
Cuts at: 2172 2172
Size: 4939
SspI AAT'ATT
Cuts at: 5 52 2863 5
Size: 47 2811 2081
Fragments arranged by size:
2811 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4939
StyI C'CwwG_G
Cuts at: 513 1065 2004 2378 513
Size: 552 939 374 3074
Fragments arranged by size:
3074 939 552 374
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2966 2983 3136 3321 3766 325
Size: 1255 1386 17 153 185 445 1498
Fragments arranged by size:
1498 1386 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3185 4897 96
Size: 274 80 33 51 1443 1208 1712 138
Fragments arranged by size:
1712 1443 1208 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2184 2216 4899 4931 2184
Size: 32 2683 32 2192
Fragments arranged by size:
2683 2192 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4939
XbaI T'CTAG_A
Cuts at: 2053 2275 2053
Size: 222 4717
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4939
XmaI C'CCGG_G
Cuts at: 1870 2170 2205 2264 1870
Size: 300 35 59 4545
Fragments arranged by size:
4545 300 59 35
XmnI GAAnn'nnTTC
Cuts at: 3068 3068
Size: 4939
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2747 276
Size: 53 83 186 936 1213 2468
Fragments arranged by size:
2468 1213 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI SalI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SwaI XcmI