Restriction map for
pFN21AE6529
(Circular) (Six-base) MAPSORT of: pFN21AE6529.seq Check: 1728 from: 1 to: 5344
pFN21AE6529 5344 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3154 278
Size: 53 83 186 936 1618 2468
Fragments arranged by size:
2468 1618 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2297 2687 1937
Size: 360 390 4594
Fragments arranged by size:
4594 390 360
Acc65I G'GTAC_C
Cuts at: 2665 2665
Size: 5344
AclI AA'CG_TT
Cuts at: 1563 3471 3844 1563
Size: 1908 373 3063
Fragments arranged by size:
3063 1908 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3407 4791 5059 1632
Size: 1775 1384 268 1917
Fragments arranged by size:
1917 1775 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2476 820
Size: 197 1459 3688
Fragments arranged by size:
3688 1459 197
AflIII A'CryG_T
Cuts at: 1203 4243 5220 1203
Size: 3040 977 1327
Fragments arranged by size:
3040 1327 977
AgeI A'CCGG_T
Cuts at: 4175 4175
Size: 5344
AhdI GACnn_n'nnGTC
Cuts at: 4073 4073
Size: 5344
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5344
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5344
AlwNI CAG_nnn'CTG
Cuts at: 3087 4659 3087
Size: 1572 3772
ApaLI G'TGCA_C
Cuts at: 3401 4557 3401
Size: 1156 4188
ApoI r'AATT_y
Cuts at: 1493 2393 2441 2653 2879 3031 5140 1493
Size: 900 48 212 226 152 2109 1697
Fragments arranged by size:
2109 1697 900 226 212 152 48
AseI AT'TA_AT
Cuts at: 160 1032 3898 160
Size: 872 2866 1606
Fragments arranged by size:
2866 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5344
AvaI C'yCGr_G
Cuts at: 1870 1943 2271 2669 1870
Size: 73 328 398 4545
Fragments arranged by size:
4545 398 328 73
BamHI G'GATC_C
Cuts at: 2674 3041 2674
Size: 367 4977
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2665 4120 618
Size: 325 217 627 878 1455 1842
Fragments arranged by size:
1842 1455 878 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2663 1391
Size: 1272 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2573 928
Size: 971 674 3699
Fragments arranged by size:
3699 971 674
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2190 2224 3535 3569 1903
Size: 34 50 34 169 34 1311 34 3678
Fragments arranged by size:
3678 1311 169 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3236 4446 3236
Size: 1210 4134
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5344
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3955 136
Size: 107 122 71 631 605 2283 1525
Fragments arranged by size:
2283 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5339 5339
Size: 5344
BlpI GC'TnA_GC
Cuts at: 2761 2761
Size: 5344
Bme1580I G_kGCm'C
Cuts at: 1790 3405 4561 1790
Size: 1615 1156 2573
Fragments arranged by size:
2573 1615 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5344
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2285 3075 4033
Size: 416 53 83 399 24 868 790 958
Cuts at: 4033 442
Size: 1753
Fragments arranged by size:
1753 958 868 790 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5344
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2173 4004 1239
Size: 934 1831 2579
Fragments arranged by size:
2579 1831 934
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3469 4334 4632 3469
Size: 865 298 4181
Fragments arranged by size:
4181 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4007 882
Size: 617 2508 2219
Fragments arranged by size:
2508 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3135 493
Size: 711 1931 2702
Fragments arranged by size:
2702 1931 711
BsaBI GATnn'nnATC
Cuts at: 5338 5338
Size: 5344
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3151 3533 4168 275
Size: 53 83 186 936 1618 382 635 1451
Fragments arranged by size:
1618 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3777 4175 4449 4596 5007 3777
Size: 398 274 147 411 4114
Fragments arranged by size:
4114 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5119
Fragments arranged by size:
5119 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2006 2186 1186
Size: 820 180 4344
Fragments arranged by size:
4344 820 180
BseYI C'CCAG_C
Cuts at: 4547 5043 4547
Size: 496 4848
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4771
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2794 3555 4583 1540
Size: 275 186 793 761 1028 2301
Fragments arranged by size:
2301 1028 793 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3405 3490 4561 3405
Size: 85 1071 4188
Fragments arranged by size:
4188 1071 85
BsmI GAATG_Cn'
Cuts at: 2440 2855 2948 2440
Size: 415 93 4836
Fragments arranged by size:
4836 415 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2663 3405 3490 4561 1391
Size: 399 873 742 85 1071 2174
Fragments arranged by size:
2174 1071 873 742 399 85
BspEI T'CCGG_A
Cuts at: 5007 5007
Size: 5344
BspHI T'CATG_A
Cuts at: 2428 3233 4963 2428
Size: 805 1730 2809
Fragments arranged by size:
2809 1730 805
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2097 2699 844
Size: 1253 602 3489
Fragments arranged by size:
3489 1253 602
BsrBI CCG'CTC
Cuts at: 1394 1823 3231 1394
Size: 429 1408 3507
Fragments arranged by size:
3507 1408 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2571 3839 4013 65
Size: 1183 1323 1268 174 1396
Fragments arranged by size:
1396 1323 1268 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3988 4175 1700
Size: 110 2178 187 2869
Fragments arranged by size:
2869 2178 187 110
BsrGI T'GTAC_A
Cuts at: 96 2552 96
Size: 2456 2888
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5344
BssSI C'ACGA_G
Cuts at: 2064 3404 4416 2064
Size: 1340 1012 2992
Fragments arranged by size:
2992 1340 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5344
BstBI TT'CG_AA
Cuts at: 2244 4148 2244
Size: 1904 3440
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5344
BstYI r'GATC_y
Cuts at: 1972 2674 3041 3426 3443 4895 4981 4993 5339
Size: 702 367 385 17 1452 86 12 346
Cuts at: 5339 1972
Size: 1977
Fragments arranged by size:
1977 1452 702 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2009 513
Size: 552 944 3848
Fragments arranged by size:
3848 944 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4605
Fragments arranged by size:
4605 683 56
BtsI GCAGTG_nn'
Cuts at: 2092 2860 3654 3674 2092
Size: 768 794 20 3762
Fragments arranged by size:
3762 794 768 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5344
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5309
DraI TTT'AAA
Cuts at: 2599 2648 2599
Size: 49 5295
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4351 809
Size: 507 3035 1802
Fragments arranged by size:
3035 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2140 2791 3680
Size: 54 1425 50 275 18 310 651 889
Cuts at: 3680 5309 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 651 310 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2791 1537
Size: 275 979 4090
Fragments arranged by size:
4090 979 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2272 3274 1353
Size: 919 1002 3423
Fragments arranged by size:
3423 1002 919
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3931 4305 4451 387
Size: 1064 138 2342 374 146 1280
Fragments arranged by size:
2342 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2173 3407 4004 4791 5059 1239
Size: 393 541 1234 597 787 268 1524
Fragments arranged by size:
1524 1234 787 597 541 393 268
EcoRI G'AATT_C
Cuts at: 2653 3031 2653
Size: 378 4966
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2582 2582
Size: 5344
FspI TGC'GCA
Cuts at: 3850 3850
Size: 5344
HaeII r_GCGC'y
Cuts at: 1678 4491 1678
Size: 2813 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3985 4017 4059 4091 1373
Size: 32 135 32 2413 32 42 32 2626
Fragments arranged by size:
2626 2413 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2688 2934 669
Size: 988 281 750 246 3079
Fragments arranged by size:
3079 988 750 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5344
HpaI GTT'AAC
Cuts at: 2934 2934
Size: 5344
KpnI G_GTAC'C
Cuts at: 2669 2669
Size: 5344
MfeI C'AATT_G
Cuts at: 2943 2943
Size: 5344
MluI A'CGCG_T
Cuts at: 5220 5220
Size: 5344
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4458 4642 1305
Size: 3153 184 2007
Fragments arranged by size:
3153 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3522
Fragments arranged by size:
3522 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2433 3302 3661 3820 518
Size: 648 1267 869 359 159 2042
Fragments arranged by size:
2042 1267 869 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2011 2760 3437 4585 4830 1606
Size: 405 749 677 1148 245 2120
Fragments arranged by size:
2120 1148 749 677 405 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4792
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5344
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5344
NotI GC'GGCC_GC
Cuts at: 2791 2791
Size: 5344
NspI r_CATG'y
Cuts at: 1132 1229 2580 2702 4247 1132
Size: 97 1351 122 1545 2229
Fragments arranged by size:
2229 1545 1351 122 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5344
PciI A'CATG_T
Cuts at: 4243 4243
Size: 5344
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5344
PmeI GTTT'AAAC
Cuts at: 2648 2648
Size: 5344
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3413 3445 4926 4958 3413
Size: 32 1481 32 3799
Fragments arranged by size:
3799 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5344
PsiI TTA'TAA
Cuts at: 2914 2914
Size: 5344
PstI C_TGCA'G
Cuts at: 830 2696 830
Size: 1866 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5344
SacII CC_GC'GG
Cuts at: 2012 2012
Size: 5344
SalI G'TCGA_C
Cuts at: 1936 2686 1936
Size: 750 4594
SbfI CC_TGCA'GG
Cuts at: 2696 2696
Size: 5344
ScaI AGT'ACT
Cuts at: 3592 5304 3592
Size: 1712 3632
SfcI C'TryA_G
Cuts at: 826 1045 2227 2692 3827 4508 4699 826
Size: 219 1182 465 1135 681 191 1471
Fragments arranged by size:
1471 1182 1135 681 465 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5344
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5344
SmaI CCC'GGG
Cuts at: 1872 2273 2671 1872
Size: 401 398 4545
Fragments arranged by size:
4545 401 398
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2476 3448 4349 4611 820
Size: 197 926 533 972 901 262 1553
Fragments arranged by size:
1553 972 926 901 533 262 197
SnaBI TAC'GTA
Cuts at: 493 3135 493
Size: 2642 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5344
SphI G_CATG'C
Cuts at: 1132 2702 1132
Size: 1570 3774
SspI AAT'ATT
Cuts at: 5 52 3268 5
Size: 47 3216 2081
Fragments arranged by size:
3216 2081 47
StuI AGG'CCT
Cuts at: 1517 2207 1517
Size: 690 4654
StyI C'CwwG_G
Cuts at: 513 1065 2377 2783 513
Size: 552 1312 406 3074
Fragments arranged by size:
3074 1312 552 406
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3371 3388 3541 3726 4171 325
Size: 1255 1791 17 153 185 445 1498
Fragments arranged by size:
1791 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2552 3590 5302
Size: 274 80 33 51 1443 575 1038 1712
Cuts at: 5302 96
Size: 138
Fragments arranged by size:
1712 1443 1038 575 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5304 5336 5304
Size: 32 5312
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5344
XbaI T'CTAG_A
Cuts at: 2680 2680
Size: 5344
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5344
XmaI C'CCGG_G
Cuts at: 1870 2271 2669 1870
Size: 401 398 4545
Fragments arranged by size:
4545 401 398
XmnI GAAnn'nnTTC
Cuts at: 3473 3473
Size: 5344
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3152 276
Size: 53 83 186 936 1618 2468
Fragments arranged by size:
2468 1618 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SacII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI