Restriction map for
pFN21AE6527
(Circular) (Six-base) MAPSORT of: pFN21AE6527.seq Check: 383 from: 1 to: 5227
pFN21AE6527 5227 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3037 278
Size: 53 83 186 936 1501 2468
Fragments arranged by size:
2468 1501 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2570 1937
Size: 633 4594
Acc65I G'GTAC_C
Cuts at: 2548 2548
Size: 5227
AclI AA'CG_TT
Cuts at: 1563 3354 3727 1563
Size: 1791 373 3063
Fragments arranged by size:
3063 1791 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2324 3290 4674 4942 1632
Size: 692 966 1384 268 1917
Fragments arranged by size:
1917 1384 966 692 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5030
AflIII A'CryG_T
Cuts at: 1203 4126 5103 1203
Size: 2923 977 1327
Fragments arranged by size:
2923 1327 977
AgeI A'CCGG_T
Cuts at: 4058 4058
Size: 5227
AhdI GACnn_n'nnGTC
Cuts at: 3956 3956
Size: 5227
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5227
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5227
AlwNI CAG_nnn'CTG
Cuts at: 2127 2437 2970 4542 2127
Size: 310 533 1572 2812
Fragments arranged by size:
2812 1572 533 310
ApaLI G'TGCA_C
Cuts at: 3284 4440 3284
Size: 1156 4071
ApoI r'AATT_y
Cuts at: 1493 2536 2762 2914 5023 1493
Size: 1043 226 152 2109 1697
Fragments arranged by size:
2109 1697 1043 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3781 160
Size: 872 2749 1606
Fragments arranged by size:
2749 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5227
AvaI C'yCGr_G
Cuts at: 1870 1943 2158 2552 1870
Size: 73 215 394 4545
Fragments arranged by size:
4545 394 215 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2417 2450 2417
Size: 33 5194
BamHI G'GATC_C
Cuts at: 2557 2924 2557
Size: 367 4860
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2548 4003 618
Size: 325 217 627 761 1455 1842
Fragments arranged by size:
1842 1455 761 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2393 2501 2546 1391
Size: 1002 108 45 4072
Fragments arranged by size:
4072 1002 108 45
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2330 928
Size: 971 431 3825
Fragments arranged by size:
3825 971 431
BbvCI CC'TCA_GC
Cuts at: 2219 2219
Size: 5227
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3418 3452 1903
Size: 34 1481 34 3678
Fragments arranged by size:
3678 1481 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3119 4329 3119
Size: 1210 4017
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5227
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3838 136
Size: 107 122 71 631 605 2166 1525
Fragments arranged by size:
2166 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5222 5222
Size: 5227
BlpI GC'TnA_GC
Cuts at: 2644 2644
Size: 5227
Bme1580I G_kGCm'C
Cuts at: 1790 3288 4444 1790
Size: 1498 1156 2573
Fragments arranged by size:
2573 1498 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5227
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2482 2958 3916
Size: 416 53 83 399 24 1065 476 958
Cuts at: 3916 442
Size: 1753
Fragments arranged by size:
1753 1065 958 476 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5227
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2347 2347
Size: 5227
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2150 2405 3887 1239
Size: 911 255 1482 2579
Fragments arranged by size:
2579 1482 911 255
Bpu10I CC'TnA_GC
Cuts at: 2219 2219
Size: 5227
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2113 3352 4217 4515 2113
Size: 1239 865 298 2825
Fragments arranged by size:
2825 1239 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3890 882
Size: 617 2391 2219
Fragments arranged by size:
2391 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3018 493
Size: 711 1814 2702
Fragments arranged by size:
2702 1814 711
BsaBI GATnn'nnATC
Cuts at: 5221 5221
Size: 5227
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3034 3416 4051 275
Size: 53 83 186 936 1501 382 635 1451
Fragments arranged by size:
1501 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3660 4058 4332 4479 4890 3660
Size: 398 274 147 411 3997
Fragments arranged by size:
3997 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5002
Fragments arranged by size:
5002 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2521 1186
Size: 1335 3892
BseYI C'CCAG_C
Cuts at: 4430 4926 4430
Size: 496 4731
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4654
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2677 3438 4466 1540
Size: 275 186 676 761 1028 2301
Fragments arranged by size:
2301 1028 761 676 275 186
BsiHKAI G_wGCw'C
Cuts at: 3288 3373 4444 3288
Size: 85 1071 4071
Fragments arranged by size:
4071 1071 85
BsmI GAATG_Cn'
Cuts at: 2063 2491 2738 2831 2063
Size: 428 247 93 4459
Fragments arranged by size:
4459 428 247 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2393 2501 2546 3288 3373 4444 1391
Size: 399 603 108 45 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 603 399 108 85 45
BspEI T'CCGG_A
Cuts at: 4890 4890
Size: 5227
BspHI T'CATG_A
Cuts at: 3116 4846 3116
Size: 1730 3497
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2088 2445 2582 844
Size: 1244 357 137 3489
Fragments arranged by size:
3489 1244 357 137
BsrBI CCG'CTC
Cuts at: 1394 1823 2235 3114 1394
Size: 429 412 879 3507
Fragments arranged by size:
3507 879 429 412
BsrDI GCAATG_nn'
Cuts at: 65 1248 3722 3896 65
Size: 1183 2474 174 1396
Fragments arranged by size:
2474 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3871 4058 1700
Size: 110 2061 187 2869
Fragments arranged by size:
2869 2061 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5227
BssHII G'CGCG_C
Cuts at: 1925 2111 1925
Size: 186 5041
BssSI C'ACGA_G
Cuts at: 3287 4299 3287
Size: 1012 4215
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2437 794
Size: 1643 3584
BstBI TT'CG_AA
Cuts at: 4031 4031
Size: 5227
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5227
BstYI r'GATC_y
Cuts at: 1972 2557 2924 3309 3326 4778 4864 4876 5222
Size: 585 367 385 17 1452 86 12 346
Cuts at: 5222 1972
Size: 1977
Fragments arranged by size:
1977 1452 585 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2182 513
Size: 552 939 178 3558
Fragments arranged by size:
3558 939 552 178
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4488
Fragments arranged by size:
4488 683 56
BtsI GCAGTG_nn'
Cuts at: 2213 2743 3537 3557 2213
Size: 530 794 20 3883
Fragments arranged by size:
3883 794 530 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5227
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5192
DraI TTT'AAA
Cuts at: 2531 2531
Size: 5227
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4234 809
Size: 507 2918 1802
Fragments arranged by size:
2918 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2674 3563 5192
Size: 54 1425 50 275 18 844 889 1629
Cuts at: 5192 8
Size: 43
Fragments arranged by size:
1629 1425 889 844 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2674 1537
Size: 275 862 4090
Fragments arranged by size:
4090 862 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3157 1353
Size: 1804 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3814 4188 4334 387
Size: 1064 138 2225 374 146 1280
Fragments arranged by size:
2225 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2150 2324 2405 3290 3887 4674 4942
Size: 393 518 174 81 885 597 787 268
Cuts at: 4942 1239
Size: 1524
Fragments arranged by size:
1524 885 787 597 518 393 268 174
81
EcoNI CCTnn'n_nnAGG
Cuts at: 2024 2024
Size: 5227
EcoO109I rG'GnC_Cy
Cuts at: 2398 2398
Size: 5227
EcoRI G'AATT_C
Cuts at: 2536 2914 2536
Size: 378 4849
FspI TGC'GCA
Cuts at: 3733 3733
Size: 5227
HaeII r_GCGC'y
Cuts at: 1678 2464 4374 1678
Size: 786 1910 2531
Fragments arranged by size:
2531 1910 786
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3868 3900 3942 3974 1373
Size: 32 135 32 2296 32 42 32 2626
Fragments arranged by size:
2626 2296 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2571 2817 669
Size: 988 281 633 246 3079
Fragments arranged by size:
3079 988 633 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5227
HpaI GTT'AAC
Cuts at: 2817 2817
Size: 5227
KpnI G_GTAC'C
Cuts at: 2552 2552
Size: 5227
MfeI C'AATT_G
Cuts at: 2826 2826
Size: 5227
MluI A'CGCG_T
Cuts at: 5103 5103
Size: 5227
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2439 4341 4525 1305
Size: 1134 1902 184 2007
Fragments arranged by size:
2007 1902 1134 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3405
Fragments arranged by size:
3405 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3185 3544 3703 518
Size: 648 2019 359 159 2042
Fragments arranged by size:
2042 2019 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2100 2124 2184 2643 3320 4468 4713 1606
Size: 494 24 60 459 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 494 459 245 60 24
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3736
Fragments arranged by size:
3736 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5227
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5227
NotI GC'GGCC_GC
Cuts at: 2674 2674
Size: 5227
NspI r_CATG'y
Cuts at: 1132 1229 2585 4130 1132
Size: 97 1356 1545 2229
Fragments arranged by size:
2229 1545 1356 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5227
PciI A'CATG_T
Cuts at: 4126 4126
Size: 5227
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5227
PmeI GTTT'AAAC
Cuts at: 2531 2531
Size: 5227
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3296 3328 4809 4841 3296
Size: 32 1481 32 3682
Fragments arranged by size:
3682 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2398 2398
Size: 5227
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5227
PsiI TTA'TAA
Cuts at: 2797 2797
Size: 5227
PstI C_TGCA'G
Cuts at: 830 2085 2579 830
Size: 1255 494 3478
Fragments arranged by size:
3478 1255 494
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5227
RsrII CG'GwC_CG
Cuts at: 2230 2230
Size: 5227
SacII CC_GC'GG
Cuts at: 2185 2185
Size: 5227
SalI G'TCGA_C
Cuts at: 1936 2569 1936
Size: 633 4594
SbfI CC_TGCA'GG
Cuts at: 2579 2579
Size: 5227
ScaI AGT'ACT
Cuts at: 2171 3475 5187 2171
Size: 1304 1712 2211
Fragments arranged by size:
2211 1712 1304
SfcI C'TryA_G
Cuts at: 826 1045 2081 2296 2455 2575 3710 4391 4582
Size: 219 1036 215 159 120 1135 681 191
Cuts at: 4582 826
Size: 1471
Fragments arranged by size:
1471 1135 1036 681 219 215 191 159
120
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5227
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5227
SmaI CCC'GGG
Cuts at: 1872 2554 1872
Size: 682 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2092 3331 4232 4494 820
Size: 197 926 149 1239 901 262 1553
Fragments arranged by size:
1553 1239 926 901 262 197 149
SnaBI TAC'GTA
Cuts at: 493 3018 493
Size: 2525 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5227
SphI G_CATG'C
Cuts at: 1132 2585 1132
Size: 1453 3774
SspI AAT'ATT
Cuts at: 5 52 3151 5
Size: 47 3099 2081
Fragments arranged by size:
3099 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5227
StyI C'CwwG_G
Cuts at: 513 1065 2004 2666 513
Size: 552 939 662 3074
Fragments arranged by size:
3074 939 662 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2218 3254 3271 3424 3609 4054 325
Size: 1255 638 1036 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1036 638 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2169 3473 5185
Size: 274 80 33 51 1443 192 1304 1712
Cuts at: 5185 96
Size: 138
Fragments arranged by size:
1712 1443 1304 274 192 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5187 5219 5187
Size: 32 5195
Tth111I GACn'n_nGTC
Cuts at: 1316 2228 1316
Size: 912 4315
XbaI T'CTAG_A
Cuts at: 2563 2563
Size: 5227
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5227
XmaI C'CCGG_G
Cuts at: 1870 2552 1870
Size: 682 4545
XmnI GAAnn'nnTTC
Cuts at: 3356 3356
Size: 5227
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3035 276
Size: 53 83 186 936 1501 2468
Fragments arranged by size:
2468 1501 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI RsrII SacII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII
SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI