Restriction map for
pFN21AE6513
(Circular) (Six-base) MAPSORT of: pFN21AE6513.seq Check: 627 from: 1 to: 5122
pFN21AE6513 5122 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2104 2104
Size: 5122
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2932 278
Size: 53 83 186 936 1396 2468
Fragments arranged by size:
2468 1396 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2465 1937
Size: 528 4594
Acc65I G'GTAC_C
Cuts at: 2443 2443
Size: 5122
AclI AA'CG_TT
Cuts at: 1563 3249 3622 1563
Size: 1686 373 3063
Fragments arranged by size:
3063 1686 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3185 4569 4837 1632
Size: 1553 1384 268 1917
Fragments arranged by size:
1917 1553 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4925
AflIII A'CryG_T
Cuts at: 1203 4021 4998 1203
Size: 2818 977 1327
Fragments arranged by size:
2818 1327 977
AgeI A'CCGG_T
Cuts at: 3953 3953
Size: 5122
AhdI GACnn_n'nnGTC
Cuts at: 3851 3851
Size: 5122
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5122
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5122
AlwNI CAG_nnn'CTG
Cuts at: 2865 4437 2865
Size: 1572 3550
ApaLI G'TGCA_C
Cuts at: 2171 3179 4335 2171
Size: 1008 1156 2958
Fragments arranged by size:
2958 1156 1008
ApoI r'AATT_y
Cuts at: 1493 2431 2657 2809 4918 1493
Size: 938 226 152 2109 1697
Fragments arranged by size:
2109 1697 938 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3676 160
Size: 872 2644 1606
Fragments arranged by size:
2644 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5122
AvaI C'yCGr_G
Cuts at: 1870 1943 2447 1870
Size: 73 504 4545
Fragments arranged by size:
4545 504 73
BamHI G'GATC_C
Cuts at: 2452 2819 2452
Size: 367 4755
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2443 3898 618
Size: 325 217 627 656 1455 1842
Fragments arranged by size:
1842 1455 656 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2441 1391
Size: 1050 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2001 928
Size: 971 102 4049
Fragments arranged by size:
4049 971 102
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3313 3347 1903
Size: 34 1376 34 3678
Fragments arranged by size:
3678 1376 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3014 4224 3014
Size: 1210 3912
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5122
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3733 136
Size: 107 122 71 631 605 2061 1525
Fragments arranged by size:
2061 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5117 5117
Size: 5122
BlpI GC'TnA_GC
Cuts at: 2539 2539
Size: 5122
Bme1580I G_kGCm'C
Cuts at: 1790 2175 2250 3183 4339 1790
Size: 385 75 933 1156 2573
Fragments arranged by size:
2573 1156 933 385 75
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5122
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2853 3811 442
Size: 416 53 83 399 24 1436 958 1753
Fragments arranged by size:
1753 1436 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5122
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3782 1239
Size: 2543 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3247 4112 4410 3247
Size: 865 298 3959
Fragments arranged by size:
3959 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3785 882
Size: 617 2286 2219
Fragments arranged by size:
2286 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2245 2287 2913 493
Size: 711 1041 42 626 2702
Fragments arranged by size:
2702 1041 711 626 42
BsaBI GATnn'nnATC
Cuts at: 5116 5116
Size: 5122
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2929 3311 3946 275
Size: 53 83 186 936 1396 382 635 1451
Fragments arranged by size:
1451 1396 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3555 3953 4227 4374 4785 3555
Size: 398 274 147 411 3892
Fragments arranged by size:
3892 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4897
Fragments arranged by size:
4897 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2243 1186
Size: 1057 4065
BseYI C'CCAG_C
Cuts at: 4325 4821 4325
Size: 496 4626
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4549
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2572 3333 4361 1540
Size: 275 186 571 761 1028 2301
Fragments arranged by size:
2301 1028 761 571 275 186
BsiHKAI G_wGCw'C
Cuts at: 2175 3183 3268 4339 2175
Size: 1008 85 1071 2958
Fragments arranged by size:
2958 1071 1008 85
BsmI GAATG_Cn'
Cuts at: 2633 2726 2633
Size: 93 5029
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2175 2250 2441 3183 3268 4339 1391
Size: 399 385 75 191 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 385 191 85 75
BspEI T'CCGG_A
Cuts at: 4785 4785
Size: 5122
BspHI T'CATG_A
Cuts at: 2193 3011 4741 2193
Size: 818 1730 2574
Fragments arranged by size:
2574 1730 818
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2104 2477 844
Size: 1260 373 3489
Fragments arranged by size:
3489 1260 373
BsrBI CCG'CTC
Cuts at: 1394 1823 3009 1394
Size: 429 1186 3507
Fragments arranged by size:
3507 1186 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2425 3617 3791 65
Size: 1183 1177 1192 174 1396
Fragments arranged by size:
1396 1192 1183 1177 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3766 3953 1700
Size: 110 1956 187 2869
Fragments arranged by size:
2869 1956 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5122
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5122
BssSI C'ACGA_G
Cuts at: 3182 4194 3182
Size: 1012 4110
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5122
BstBI TT'CG_AA
Cuts at: 3926 3926
Size: 5122
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5122
BstYI r'GATC_y
Cuts at: 1972 2452 2819 3204 3221 4673 4759 4771 5117
Size: 480 367 385 17 1452 86 12 346
Cuts at: 5117 1972
Size: 1977
Fragments arranged by size:
1977 1452 480 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4570
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4383
Fragments arranged by size:
4383 683 56
BtsI GCAGTG_nn'
Cuts at: 2638 3432 3452 2638
Size: 794 20 4308
Fragments arranged by size:
4308 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5122
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5087
DraI TTT'AAA
Cuts at: 2426 2426
Size: 5122
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4129 809
Size: 507 2813 1802
Fragments arranged by size:
2813 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2569 3458 5087
Size: 54 1425 50 275 18 739 889 1629
Cuts at: 5087 8
Size: 43
Fragments arranged by size:
1629 1425 889 739 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2569 1537
Size: 275 757 4090
Fragments arranged by size:
4090 757 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3052 1353
Size: 1699 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3709 4083 4229 387
Size: 1064 138 2120 374 146 1280
Fragments arranged by size:
2120 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3185 3782 4569 4837 1239
Size: 393 1553 597 787 268 1524
Fragments arranged by size:
1553 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2431 2809 2431
Size: 378 4744
EcoRV GAT'ATC
Cuts at: 2392 2392
Size: 5122
FspI TGC'GCA
Cuts at: 3628 3628
Size: 5122
HaeII r_GCGC'y
Cuts at: 1678 4269 1678
Size: 2591 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3763 3795 3837 3869 1373
Size: 32 135 32 2191 32 42 32 2626
Fragments arranged by size:
2626 2191 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2466 2712 669
Size: 988 281 528 246 3079
Fragments arranged by size:
3079 988 528 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5122
HpaI GTT'AAC
Cuts at: 2712 2712
Size: 5122
KpnI G_GTAC'C
Cuts at: 2447 2447
Size: 5122
MfeI C'AATT_G
Cuts at: 2721 2721
Size: 5122
MluI A'CGCG_T
Cuts at: 4998 4998
Size: 5122
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2205 4236 4420 1305
Size: 900 2031 184 2007
Fragments arranged by size:
2031 2007 900 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3300
Fragments arranged by size:
3300 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2132 2295 3080 3439 3598 518
Size: 648 966 163 785 359 159 2042
Fragments arranged by size:
2042 966 785 648 359 163 159
MspA1I CmG'CkG
Cuts at: 1606 2110 2538 3215 4363 4608 1606
Size: 504 428 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 504 428 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4570
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5122
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5122
NotI GC'GGCC_GC
Cuts at: 2569 2569
Size: 5122
NspI r_CATG'y
Cuts at: 1132 1229 2480 4025 1132
Size: 97 1251 1545 2229
Fragments arranged by size:
2229 1545 1251 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5122
PciI A'CATG_T
Cuts at: 4021 4021
Size: 5122
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5122
PmeI GTTT'AAAC
Cuts at: 2426 2426
Size: 5122
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3191 3223 4704 4736 3191
Size: 32 1481 32 3577
Fragments arranged by size:
3577 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5122
PsiI TTA'TAA
Cuts at: 2692 2692
Size: 5122
PstI C_TGCA'G
Cuts at: 830 2115 2474 830
Size: 1285 359 3478
Fragments arranged by size:
3478 1285 359
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5122
PvuII CAG'CTG
Cuts at: 2110 2110
Size: 5122
SalI G'TCGA_C
Cuts at: 1936 2464 1936
Size: 528 4594
SbfI CC_TGCA'GG
Cuts at: 2474 2474
Size: 5122
ScaI AGT'ACT
Cuts at: 3370 5082 3370
Size: 1712 3410
SfcI C'TryA_G
Cuts at: 826 1045 2111 2470 3605 4286 4477 826
Size: 219 1066 359 1135 681 191 1471
Fragments arranged by size:
1471 1135 1066 681 359 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5122
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5122
SmaI CCC'GGG
Cuts at: 1872 2449 1872
Size: 577 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3226 4127 4389 820
Size: 197 926 1283 901 262 1553
Fragments arranged by size:
1553 1283 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2287 2913 493
Size: 1794 626 2702
Fragments arranged by size:
2702 1794 626
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5122
SphI G_CATG'C
Cuts at: 1132 2480 1132
Size: 1348 3774
SspI AAT'ATT
Cuts at: 5 52 3046 5
Size: 47 2994 2081
Fragments arranged by size:
2994 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5122
StyI C'CwwG_G
Cuts at: 513 1065 2561 513
Size: 552 1496 3074
Fragments arranged by size:
3074 1496 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3149 3166 3319 3504 3949 325
Size: 1255 1569 17 153 185 445 1498
Fragments arranged by size:
1569 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3368 5080 96
Size: 274 80 33 51 1443 1391 1712 138
Fragments arranged by size:
1712 1443 1391 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5082 5114 5082
Size: 32 5090
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5122
XbaI T'CTAG_A
Cuts at: 2458 2458
Size: 5122
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5122
XmaI C'CCGG_G
Cuts at: 1870 2447 1870
Size: 577 4545
XmnI GAAnn'nnTTC
Cuts at: 3251 3251
Size: 5122
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2930 276
Size: 53 83 186 936 1396 2468
Fragments arranged by size:
2468 1396 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI