(Circular) (Six-base) MAPSORT of: pFN21AE6507.seq Check: 2342 from: 1 to: 5335 pFN21AE6507 5335 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3145 278 Size: 53 83 186 936 1609 2468 Fragments arranged by size: 2468 1609 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2678 1937 Size: 741 4594 Acc65I G'GTAC_C Cuts at: 2656 2656 Size: 5335 AclI AA'CG_TT Cuts at: 1563 3462 3835 1563 Size: 1899 373 3063 Fragments arranged by size: 3063 1899 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3398 4782 5050 1632 Size: 1766 1384 268 1917 Fragments arranged by size: 1917 1766 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5138 AflIII A'CryG_T Cuts at: 1203 2358 4234 5211 1203 Size: 1155 1876 977 1327 Fragments arranged by size: 1876 1327 1155 977 AgeI A'CCGG_T Cuts at: 4166 4166 Size: 5335 AhdI GACnn_n'nnGTC Cuts at: 2542 4064 2542 Size: 1522 3813 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5335 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5335 AlwNI CAG_nnn'CTG Cuts at: 3078 4650 3078 Size: 1572 3763 ApaLI G'TGCA_C Cuts at: 3392 4548 3392 Size: 1156 4179 ApoI r'AATT_y Cuts at: 1493 2233 2644 2870 3022 5131 1493 Size: 740 411 226 152 2109 1697 Fragments arranged by size: 2109 1697 740 411 226 152 AseI AT'TA_AT Cuts at: 160 1032 3889 160 Size: 872 2857 1606 Fragments arranged by size: 2857 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5335 AvaI C'yCGr_G Cuts at: 1870 1943 2015 2079 2660 1870 Size: 73 72 64 581 4545 Fragments arranged by size: 4545 581 73 72 64 BamHI G'GATC_C Cuts at: 2665 3032 2665 Size: 367 4968 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2019 2656 4111 618 Size: 325 217 627 232 637 1455 1842 Fragments arranged by size: 1842 1455 637 627 325 232 217 BanII G_rGCy'C Cuts at: 1391 2574 2654 1391 Size: 1183 80 4072 Fragments arranged by size: 4072 1183 80 BbeI G_GCGC'C Cuts at: 2023 2023 Size: 5335 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2343 2598 928 Size: 971 444 255 3665 Fragments arranged by size: 3665 971 444 255 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2122 2126 2156 2160 3526 3560 1903 Size: 34 185 4 30 4 1366 34 3678 Fragments arranged by size: 3678 1366 185 34 34 30 4 4 BciVI GTATCCnnnnn_n' Cuts at: 3227 4437 3227 Size: 1210 4125 BclI T'GATC_A Cuts at: 1548 2436 1548 Size: 888 4447 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2124 2265 3946 Size: 107 122 71 631 605 452 141 1681 Cuts at: 3946 136 Size: 1525 Fragments arranged by size: 1681 1525 631 605 452 141 122 107 71 BglII A'GATC_T Cuts at: 2419 5330 2419 Size: 2911 2424 BlpI GC'TnA_GC Cuts at: 2752 2752 Size: 5335 Bme1580I G_kGCm'C Cuts at: 1790 3396 4552 1790 Size: 1606 1156 2573 Fragments arranged by size: 2573 1606 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5335 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2420 2520 2530 Size: 416 53 83 399 24 1003 100 10 Cuts at: 2530 3066 4024 442 Size: 536 958 1753 Fragments arranged by size: 1753 1003 958 536 416 399 100 83 53 24 10 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5335 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3995 1239 Size: 2756 2579 Bpu10I CC'TnA_GC Cuts at: 2493 2493 Size: 5335 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3460 4325 4623 3460 Size: 865 298 4172 Fragments arranged by size: 4172 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2273 3998 882 Size: 617 774 1725 2219 Fragments arranged by size: 2219 1725 774 617 BsaAI yAC'GTr Cuts at: 493 1204 3126 493 Size: 711 1922 2702 Fragments arranged by size: 2702 1922 711 BsaBI GATnn'nnATC Cuts at: 5329 5329 Size: 5335 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2020 2045 2450 3142 Size: 53 83 186 936 487 25 405 692 Cuts at: 3142 3524 4159 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 692 635 487 405 382 186 83 53 25 BsaWI w'CCGG_w Cuts at: 2621 3768 4166 4440 4587 4998 2621 Size: 1147 398 274 147 411 2958 Fragments arranged by size: 2958 1147 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5110 Fragments arranged by size: 5110 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5335 BseYI C'CCAG_C Cuts at: 2051 4538 5034 2051 Size: 2487 496 2352 Fragments arranged by size: 2487 2352 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2137 1163 Size: 573 401 4361 Fragments arranged by size: 4361 573 401 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2093 2119 2785 3546 4574 1540 Size: 275 186 92 26 666 761 1028 2301 Fragments arranged by size: 2301 1028 761 666 275 186 92 26 BsiHKAI G_wGCw'C Cuts at: 2574 3396 3481 4552 2574 Size: 822 85 1071 3357 Fragments arranged by size: 3357 1071 822 85 BsmI GAATG_Cn' Cuts at: 2846 2939 2846 Size: 93 5242 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2574 2654 3396 3481 4552 1391 Size: 399 784 80 742 85 1071 2174 Fragments arranged by size: 2174 1071 784 742 399 85 80 BspEI T'CCGG_A Cuts at: 2621 4998 2621 Size: 2377 2958 BspHI T'CATG_A Cuts at: 3224 4954 3224 Size: 1730 3605 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2159 2690 844 Size: 1315 531 3489 Fragments arranged by size: 3489 1315 531 BsrBI CCG'CTC Cuts at: 1394 1823 3222 1394 Size: 429 1399 3507 Fragments arranged by size: 3507 1399 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3830 4004 65 Size: 1183 2582 174 1396 Fragments arranged by size: 2582 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2033 2092 3979 4166 1700 Size: 110 223 59 1887 187 2869 Fragments arranged by size: 2869 1887 223 187 110 59 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5335 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5335 BssSI C'ACGA_G Cuts at: 3395 4407 3395 Size: 1012 4323 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5335 BstBI TT'CG_AA Cuts at: 2174 4139 2174 Size: 1965 3370 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5335 BstYI r'GATC_y Cuts at: 1972 2419 2665 3032 3417 3434 4886 4972 4984 Size: 447 246 367 385 17 1452 86 12 Cuts at: 4984 5330 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 447 385 367 346 246 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2087 2122 513 Size: 552 1022 35 3726 Fragments arranged by size: 3726 1022 552 35 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4596 Fragments arranged by size: 4596 683 56 BtsI GCAGTG_nn' Cuts at: 2851 3645 3665 2851 Size: 794 20 4521 Fragments arranged by size: 4521 794 20 ClaI AT'CG_AT Cuts at: 1554 2142 1554 Size: 588 4747 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2395 2430 529 Size: 35 1831 35 3434 Fragments arranged by size: 3434 1831 35 35 DraI TTT'AAA Cuts at: 2639 2639 Size: 5335 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2375 4342 809 Size: 507 1059 1967 1802 Fragments arranged by size: 1967 1802 1059 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2090 2116 2562 Size: 54 1425 50 275 18 260 26 446 Cuts at: 2562 2782 3671 5300 8 Size: 220 889 1629 43 Fragments arranged by size: 1629 1425 889 446 275 260 220 54 50 43 26 18 EagI C'GGCC_G Cuts at: 1537 1812 2090 2116 2782 1537 Size: 275 278 26 666 4090 Fragments arranged by size: 4090 666 278 275 26 EarI CTCTTCn'nnn_ Cuts at: 1353 3265 1353 Size: 1912 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3922 4296 4442 387 Size: 1064 138 2333 374 146 1280 Fragments arranged by size: 2333 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2572 2572 Size: 5335 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3398 3995 4782 5050 1239 Size: 393 1766 597 787 268 1524 Fragments arranged by size: 1766 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2083 2083 Size: 5335 EcoRI G'AATT_C Cuts at: 2644 3022 2644 Size: 378 4957 FspI TGC'GCA Cuts at: 2132 3841 2132 Size: 1709 3626 HaeII r_GCGC'y Cuts at: 1678 2023 4482 1678 Size: 345 2459 2531 Fragments arranged by size: 2531 2459 345 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2528 2560 3976 4008 4050 Size: 32 135 32 956 32 1416 32 42 Cuts at: 4050 4082 1373 Size: 32 2626 Fragments arranged by size: 2626 1416 956 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2679 2925 669 Size: 988 281 741 246 3079 Fragments arranged by size: 3079 988 741 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5335 HpaI GTT'AAC Cuts at: 2925 2925 Size: 5335 KasI G'GCGC_C Cuts at: 2019 2019 Size: 5335 KpnI G_GTAC'C Cuts at: 2660 2660 Size: 5335 MfeI C'AATT_G Cuts at: 2934 2934 Size: 5335 MluI A'CGCG_T Cuts at: 5211 5211 Size: 5335 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4449 4633 1305 Size: 3144 184 2007 Fragments arranged by size: 3144 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3513 Fragments arranged by size: 3513 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3293 3652 3811 518 Size: 648 2127 359 159 2042 Fragments arranged by size: 2127 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2089 2751 3428 4576 4821 1606 Size: 483 662 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 662 483 245 NaeI GCC'GGC Cuts at: 2035 2094 2035 Size: 59 5276 NarI GG'CG_CC Cuts at: 2020 2020 Size: 5335 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4783 NdeI CA'TA_TG Cuts at: 387 387 Size: 5335 NgoMIV G'CCGG_C Cuts at: 2033 2092 2033 Size: 59 5276 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5335 NotI GC'GGCC_GC Cuts at: 2116 2782 2116 Size: 666 4669 NspI r_CATG'y Cuts at: 1132 1229 2693 4238 1132 Size: 97 1464 1545 2229 Fragments arranged by size: 2229 1545 1464 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5335 PciI A'CATG_T Cuts at: 4234 4234 Size: 5335 PflMI CCAn_nnn'nTGG Cuts at: 1108 2486 1108 Size: 1378 3957 PmeI GTTT'AAAC Cuts at: 2639 2639 Size: 5335 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3404 3436 4917 4949 3404 Size: 32 1481 32 3790 Fragments arranged by size: 3790 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2083 2083 Size: 5335 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5335 PsiI TTA'TAA Cuts at: 2905 2905 Size: 5335 PstI C_TGCA'G Cuts at: 830 2687 830 Size: 1857 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5335 SacI G_AGCT'C Cuts at: 2574 2574 Size: 5335 SacII CC_GC'GG Cuts at: 2090 2090 Size: 5335 SalI G'TCGA_C Cuts at: 1936 2677 1936 Size: 741 4594 SanDI GG'GwC_CC Cuts at: 2083 2083 Size: 5335 SbfI CC_TGCA'GG Cuts at: 2687 2687 Size: 5335 ScaI AGT'ACT Cuts at: 3583 5295 3583 Size: 1712 3623 SfcI C'TryA_G Cuts at: 826 1045 2683 3818 4499 4690 826 Size: 219 1638 1135 681 191 1471 Fragments arranged by size: 1638 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2124 2124 Size: 5335 SfoI GGC'GCC Cuts at: 2021 2021 Size: 5335 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5335 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5335 SmaI CCC'GGG Cuts at: 1872 2081 2662 1872 Size: 209 581 4545 Fragments arranged by size: 4545 581 209 SmlI C'TyrA_G Cuts at: 820 1017 1943 3439 4340 4602 820 Size: 197 926 1496 901 262 1553 Fragments arranged by size: 1553 1496 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3126 493 Size: 2633 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5335 SphI G_CATG'C Cuts at: 1132 2693 1132 Size: 1561 3774 SspI AAT'ATT Cuts at: 5 52 3259 5 Size: 47 3207 2081 Fragments arranged by size: 3207 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5335 StyI C'CwwG_G Cuts at: 513 1065 2610 2774 513 Size: 552 1545 164 3074 Fragments arranged by size: 3074 1545 552 164 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2065 2277 2402 2512 3362 3379 3532 Size: 1255 485 212 125 110 850 17 153 Cuts at: 3532 3717 4162 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 850 485 445 212 185 153 125 110 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2526 3581 5293 Size: 274 80 33 51 1443 549 1055 1712 Cuts at: 5293 96 Size: 138 Fragments arranged by size: 1712 1443 1055 549 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2418 2450 5295 5327 2418 Size: 32 2845 32 2426 Fragments arranged by size: 2845 2426 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5335 XbaI T'CTAG_A Cuts at: 2272 2671 2272 Size: 399 4936 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5335 XmaI C'CCGG_G Cuts at: 1870 2079 2660 1870 Size: 209 581 4545 Fragments arranged by size: 4545 581 209 XmnI GAAnn'nnTTC Cuts at: 3464 3464 Size: 5335 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3143 276 Size: 53 83 186 936 1609 2468 Fragments arranged by size: 2468 1609 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SapI SexAI SrfI SwaI XcmI