Restriction map for
pFN21AE6495
(Circular) (Six-base) MAPSORT of: pFN21AE6495.seq Check: 7556 from: 1 to: 6079
pFN21AE6495 6079 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2023 2182 2023
Size: 159 5920
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3889 278
Size: 53 83 186 936 2353 2468
Fragments arranged by size:
2468 2353 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3422 1937
Size: 1485 4594
Acc65I G'GTAC_C
Cuts at: 3400 3400
Size: 6079
AclI AA'CG_TT
Cuts at: 1563 4206 4579 1563
Size: 2643 373 3063
Fragments arranged by size:
3063 2643 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4142 5526 5794 1632
Size: 2510 1384 268 1917
Fragments arranged by size:
2510 1917 1384 268
AfeI AGC'GCT
Cuts at: 2760 2834 3180 2760
Size: 74 346 5659
Fragments arranged by size:
5659 346 74
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5882
AflIII A'CryG_T
Cuts at: 1203 2364 2559 2988 4978 5955 1203
Size: 1161 195 429 1990 977 1327
Fragments arranged by size:
1990 1327 1161 977 429 195
AgeI A'CCGG_T
Cuts at: 4910 4910
Size: 6079
AhdI GACnn_n'nnGTC
Cuts at: 4808 4808
Size: 6079
AleI CACnn'nnGTG
Cuts at: 1166 2960 3191 1166
Size: 1794 231 4054
Fragments arranged by size:
4054 1794 231
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2347 1184
Size: 1163 4916
AlwNI CAG_nnn'CTG
Cuts at: 2026 2197 3822 5394 2026
Size: 171 1625 1572 2711
Fragments arranged by size:
2711 1625 1572 171
ApaLI G'TGCA_C
Cuts at: 4136 5292 4136
Size: 1156 4923
ApoI r'AATT_y
Cuts at: 1493 3388 3614 3766 5875 1493
Size: 1895 226 152 2109 1697
Fragments arranged by size:
2109 1895 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4633 160
Size: 872 3601 1606
Fragments arranged by size:
3601 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6079
AvaI C'yCGr_G
Cuts at: 1870 1943 3404 1870
Size: 73 1461 4545
Fragments arranged by size:
4545 1461 73
BamHI G'GATC_C
Cuts at: 3409 3776 3409
Size: 367 5712
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2272 2660 3260 3400 4855
Size: 325 217 627 485 388 600 140 1455
Cuts at: 4855 618
Size: 1842
Fragments arranged by size:
1842 1455 627 600 485 388 325 217
140
BanII G_rGCy'C
Cuts at: 1391 3398 1391
Size: 2007 4072
BbeI G_GCGC'C
Cuts at: 2276 2664 2276
Size: 388 5691
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5108
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4270 4304 1903
Size: 34 2333 34 3678
Fragments arranged by size:
3678 2333 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3971 5181 3971
Size: 1210 4869
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6079
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2423 2450 2615
Size: 107 122 71 631 605 751 27 165
Cuts at: 2615 4690 136
Size: 2075 1525
Fragments arranged by size:
2075 1525 751 631 605 165 122 107
71 27
BglII A'GATC_T
Cuts at: 6074 6074
Size: 6079
BlpI GC'TnA_GC
Cuts at: 3496 3496
Size: 6079
Bme1580I G_kGCm'C
Cuts at: 1790 2901 4140 5296 1790
Size: 1111 1239 1156 2573
Fragments arranged by size:
2573 1239 1156 1111
BmgBI CAC'GTC
Cuts at: 1318 2310 2365 1318
Size: 992 55 5032
Fragments arranged by size:
5032 992 55
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3810 4768 442
Size: 416 53 83 399 24 2393 958 1753
Fragments arranged by size:
2393 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6079
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2539 2539
Size: 6079
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2252 2315 2534 2609 2878 4739 1239
Size: 1013 63 219 75 269 1861 2579
Fragments arranged by size:
2579 1861 1013 269 219 75 63
Bpu10I CC'TnA_GC
Cuts at: 3275 3275
Size: 6079
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4204 5069 5367 4204
Size: 865 298 4916
Fragments arranged by size:
4916 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2123 4742 882
Size: 617 624 2619 2219
Fragments arranged by size:
2619 2219 624 617
BsaAI yAC'GTr
Cuts at: 493 1204 2449 2715 3870 493
Size: 711 1245 266 1155 2702
Fragments arranged by size:
2702 1245 1155 711 266
BsaBI GATnn'nnATC
Cuts at: 6073 6073
Size: 6079
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2273 2520 2661 2813
Size: 53 83 186 936 740 247 141 152
Cuts at: 2813 3016 3886 4268 4903 275
Size: 203 870 382 635 1451
Fragments arranged by size:
1451 936 870 740 635 382 247 203
186 152 141 83 53
BsaWI w'CCGG_w
Cuts at: 4512 4910 5184 5331 5742 4512
Size: 398 274 147 411 4849
Fragments arranged by size:
4849 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5854
Fragments arranged by size:
5854 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6079
BseYI C'CCAG_C
Cuts at: 2465 2623 2683 2729 2842 5282 5778 2465
Size: 158 60 46 113 2440 496 2766
Fragments arranged by size:
2766 2440 496 158 113 60 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2051 3083 1163
Size: 573 315 1032 4159
Fragments arranged by size:
4159 1032 573 315
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2068 2209 2213 2343 2418 2481
Size: 275 186 67 141 4 130 75 63
Cuts at: 2481 2779 2889 2945 2949 3192 3529 4290 5318
Size: 298 110 56 4 243 337 761 1028
Cuts at: 5318 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 337 298 275 243 186
141 130 110 75 67 63 56 4
4
BsiHKAI G_wGCw'C
Cuts at: 2088 2301 2489 4140 4225 5296 2088
Size: 213 188 1651 85 1071 2871
Fragments arranged by size:
2871 1651 1071 213 188 85
BsmI GAATG_Cn'
Cuts at: 3590 3683 3590
Size: 93 5986
BsmBI CGTCTCn'nnnn_
Cuts at: 2927 2927
Size: 6079
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2088 2301 2489 2901 3398 4140 4225
Size: 399 298 213 188 412 497 742 85
Cuts at: 4225 5296 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 497 412 399 298 213
188 85
BspEI T'CCGG_A
Cuts at: 5742 5742
Size: 6079
BspHI T'CATG_A
Cuts at: 3968 5698 3968
Size: 1730 4349
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2023 2182 2233 2883 3434 844
Size: 1179 159 51 650 551 3489
Fragments arranged by size:
3489 1179 650 551 159 51
BsrBI CCG'CTC
Cuts at: 1394 1823 2115 2381 3966 1394
Size: 429 292 266 1585 3507
Fragments arranged by size:
3507 1585 429 292 266
BsrDI GCAATG_nn'
Cuts at: 65 1248 2324 4574 4748 65
Size: 1183 1076 2250 174 1396
Fragments arranged by size:
2250 1396 1183 1076 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2208 2212 2275 2765 2884 2891 2944
Size: 110 398 4 63 490 119 7 53
Cuts at: 2944 3287 4723 4910 1700
Size: 343 1436 187 2869
Fragments arranged by size:
2869 1436 490 398 343 187 119 110
63 53 7 4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6079
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6079
BssSI C'ACGA_G
Cuts at: 2720 4139 5151 2720
Size: 1419 1012 3648
Fragments arranged by size:
3648 1419 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2197 794
Size: 1403 4676
BstBI TT'CG_AA
Cuts at: 4883 4883
Size: 6079
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6079
BstXI CCAn_nnnn'nTGG
Cuts at: 2915 2962 2915
Size: 47 6032
BstYI r'GATC_y
Cuts at: 1972 3409 3776 4161 4178 5630 5716 5728 6074
Size: 1437 367 385 17 1452 86 12 346
Cuts at: 6074 1972
Size: 1977
Fragments arranged by size:
1977 1452 1437 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2433 3326 2433
Size: 893 5186
BtgI C'CryG_G
Cuts at: 513 1065 2004 2826 2847 2961 3129 3192 3296
Size: 552 939 822 21 114 168 63 104
Cuts at: 3296 513
Size: 3296
Fragments arranged by size:
3296 939 822 552 168 114 104 63
21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5340
Fragments arranged by size:
5340 683 56
BtsI GCAGTG_nn'
Cuts at: 2982 3034 3100 3595 4389 4409 2982
Size: 52 66 495 794 20 4652
Fragments arranged by size:
4652 794 495 66 52 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6079
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6044
DraI TTT'AAA
Cuts at: 3383 3383
Size: 6079
DraIII CAC_nnn'GTG
Cuts at: 2535 2535
Size: 6079
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5086 809
Size: 507 3770 1802
Fragments arranged by size:
3770 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2047 2210 2340
Size: 54 1425 50 275 18 217 163 130
Cuts at: 2340 2415 2478 2607 2616 2763 2776 2868 2886
Size: 75 63 129 9 147 13 92 18
Cuts at: 2886 2917 2942 2946 3003 3031 3126 3154 3189
Size: 31 25 4 57 28 95 28 35
Cuts at: 3189 3526 4415 6044 8
Size: 337 889 1629 43
Fragments arranged by size:
1629 1425 889 337 275 217 163 147
130 129 95 92 75 63 57 54
50 43 35 31 28 28 25 18
18 13 9 4
EagI C'GGCC_G
Cuts at: 1537 1812 2210 2340 2415 2478 2776 2886 2942
Size: 275 398 130 75 63 298 110 56
Cuts at: 2942 2946 3189 3526 1537
Size: 4 243 337 4090
Fragments arranged by size:
4090 398 337 298 275 243 130 110
75 63 56 4
EarI CTCTTCn'nnn_
Cuts at: 1353 2060 2114 2390 2547 2930 3188 4009 1353
Size: 707 54 276 157 383 258 821 3423
Fragments arranged by size:
3423 821 707 383 276 258 157 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2240 4666 5040 5186 387
Size: 1064 138 651 2426 374 146 1280
Fragments arranged by size:
2426 1280 1064 651 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2252 2315 2534 2609 2878 4142 4739
Size: 393 620 63 219 75 269 1264 597
Cuts at: 4739 5526 5794 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1264 787 620 597 393 269 268
219 75 63
EcoNI CCTnn'n_nnAGG
Cuts at: 2030 2546 2030
Size: 516 5563
EcoRI G'AATT_C
Cuts at: 3388 3766 3388
Size: 378 5701
FseI GG_CCGG'CC
Cuts at: 2948 2948
Size: 6079
FspI TGC'GCA
Cuts at: 2323 3074 4585 2323
Size: 751 1511 3817
Fragments arranged by size:
3817 1511 751
HaeII r_GCGC'y
Cuts at: 1678 2219 2276 2282 2429 2664 2762 2836 3149
Size: 541 57 6 147 235 98 74 313
Cuts at: 3149 3182 3345 5226 1678
Size: 33 163 1881 2531
Fragments arranged by size:
2531 1881 541 313 235 163 147 98
74 57 33 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4720 4752 4794 4826 1373
Size: 32 135 32 3148 32 42 32 2626
Fragments arranged by size:
3148 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3166 3223 3423 3669 669
Size: 988 281 1228 57 200 246 3079
Fragments arranged by size:
3079 1228 988 281 246 200 57
HindIII A'AGCT_T
Cuts at: 748 2633 748
Size: 1885 4194
HpaI GTT'AAC
Cuts at: 3669 3669
Size: 6079
KasI G'GCGC_C
Cuts at: 2272 2660 2272
Size: 388 5691
KpnI G_GTAC'C
Cuts at: 3404 3404
Size: 6079
MfeI C'AATT_G
Cuts at: 3678 3678
Size: 6079
MluI A'CGCG_T
Cuts at: 2559 2988 5955 2559
Size: 429 2967 2683
Fragments arranged by size:
2967 2683 429
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2292 3111 5193 5377 1305
Size: 987 819 2082 184 2007
Fragments arranged by size:
2082 2007 987 819 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2618 3033 3128 10
Size: 54 1425 343 786 415 95 2961
Fragments arranged by size:
2961 1425 786 415 343 95 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2329 2960 3191 4037 4396 4555 518
Size: 648 1163 631 231 846 359 159 2042
Fragments arranged by size:
2042 1163 846 648 631 359 231 159
MspA1I CmG'CkG
Cuts at: 1606 2849 3298 3495 4172 5320 5565 1606
Size: 1243 449 197 677 1148 245 2120
Fragments arranged by size:
2120 1243 1148 677 449 245 197
NaeI GCC'GGC
Cuts at: 2214 2277 2767 2886 2893 2946 3289 2214
Size: 63 490 119 7 53 343 5004
Fragments arranged by size:
5004 490 343 119 63 53 7
NarI GG'CG_CC
Cuts at: 2273 2661 2273
Size: 388 5691
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4588
Fragments arranged by size:
4588 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6079
NgoMIV G'CCGG_C
Cuts at: 2212 2275 2765 2884 2891 2944 3287 2212
Size: 63 490 119 7 53 343 5004
Fragments arranged by size:
5004 490 343 119 63 53 7
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6079
NotI GC'GGCC_GC
Cuts at: 2340 3526 2340
Size: 1186 4893
NspI r_CATG'y
Cuts at: 1132 1229 2125 3232 3437 4982 1132
Size: 97 896 1107 205 1545 2229
Fragments arranged by size:
2229 1545 1107 896 205 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6079
PciI A'CATG_T
Cuts at: 4978 4978
Size: 6079
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3040 1108
Size: 1932 4147
PfoI T'CCnGG_A
Cuts at: 2511 2511
Size: 6079
PmeI GTTT'AAAC
Cuts at: 3383 3383
Size: 6079
PmlI CAC'GTG
Cuts at: 2715 2715
Size: 6079
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4148 4180 5661 5693 4148
Size: 32 1481 32 4534
Fragments arranged by size:
4534 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6079
PsiI TTA'TAA
Cuts at: 3649 3649
Size: 6079
PstI C_TGCA'G
Cuts at: 830 2193 2244 3431 830
Size: 1363 51 1187 3478
Fragments arranged by size:
3478 1363 1187 51
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6079
RsrII CG'GwC_CG
Cuts at: 2982 2982
Size: 6079
SacII CC_GC'GG
Cuts at: 2850 3299 2850
Size: 449 5630
SalI G'TCGA_C
Cuts at: 1936 3421 1936
Size: 1485 4594
SapI GCTCTTCn'nnn_
Cuts at: 2060 3188 2060
Size: 1128 4951
SbfI CC_TGCA'GG
Cuts at: 3431 3431
Size: 6079
ScaI AGT'ACT
Cuts at: 4327 6039 4327
Size: 1712 4367
SfcI C'TryA_G
Cuts at: 826 1045 2189 2240 3427 4562 5243 5434 826
Size: 219 1144 51 1187 1135 681 191 1471
Fragments arranged by size:
1471 1187 1144 1135 681 219 191 51
SfiI GGCCn_nnn'nGGCC
Cuts at: 2615 2615
Size: 6079
SfoI GGC'GCC
Cuts at: 2274 2662 2274
Size: 388 5691
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6079
SgrAI Cr'CCGG_yG
Cuts at: 1700 2275 2891 1700
Size: 575 616 4888
Fragments arranged by size:
4888 616 575
SmaI CCC'GGG
Cuts at: 1872 3406 1872
Size: 1534 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4183 5084 5346 820
Size: 197 926 2240 901 262 1553
Fragments arranged by size:
2240 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3870 493
Size: 3377 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6079
SphI G_CATG'C
Cuts at: 1132 2125 3437 1132
Size: 993 1312 3774
Fragments arranged by size:
3774 1312 993
SspI AAT'ATT
Cuts at: 5 52 4003 5
Size: 47 3951 2081
Fragments arranged by size:
3951 2081 47
StuI AGG'CCT
Cuts at: 1517 2106 1517
Size: 589 5490
StyI C'CwwG_G
Cuts at: 513 1065 2004 3518 513
Size: 552 939 1514 3074
Fragments arranged by size:
3074 1514 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2029 2999 4106 4123 4276 4461 4906
Size: 1255 449 970 1107 17 153 185 445
Cuts at: 4906 325
Size: 1498
Fragments arranged by size:
1498 1255 1107 970 449 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4325 6037 96
Size: 274 80 33 51 1443 2348 1712 138
Fragments arranged by size:
2348 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6039 6071 6039
Size: 32 6047
Tth111I GACn'n_nGTC
Cuts at: 1316 3209 1316
Size: 1893 4186
XbaI T'CTAG_A
Cuts at: 3415 3415
Size: 6079
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2602 2671 2824 2962 2602
Size: 69 153 138 5719
Fragments arranged by size:
5719 153 138 69
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6079
XmaI C'CCGG_G
Cuts at: 1870 3404 1870
Size: 1534 4545
XmnI GAAnn'nnTTC
Cuts at: 4208 4208
Size: 6079
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3887 276
Size: 53 83 186 936 2353 2468
Fragments arranged by size:
2468 2353 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoRI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PstI PvuI RsrII SacII SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI
BstZ17I EcoICRI EcoO109I EcoRV FalI FspAI NruI NsiI
PacI PpuMI PspOMI PspXI PsrI PvuII SacI SanDI
SexAI SrfI SwaI