Restriction map for
pFN21AE6493
(Circular) (Six-base) MAPSORT of: pFN21AE6493.seq Check: 7088 from: 1 to: 6535
pFN21AE6493 6535 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4345 278
Size: 53 83 186 936 2809 2468
Fragments arranged by size:
2809 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2238 2429 3878 1937
Size: 301 191 1449 4594
Fragments arranged by size:
4594 1449 301 191
Acc65I G'GTAC_C
Cuts at: 2916 3856 2916
Size: 940 5595
AclI AA'CG_TT
Cuts at: 1563 4662 5035 1563
Size: 3099 373 3063
Fragments arranged by size:
3099 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3106 3559 4598 5982 6250 1632
Size: 1474 453 1039 1384 268 1917
Fragments arranged by size:
1917 1474 1384 1039 453 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6338
AflIII A'CryG_T
Cuts at: 1203 5434 6411 1203
Size: 4231 977 1327
Fragments arranged by size:
4231 1327 977
AgeI A'CCGG_T
Cuts at: 5366 5366
Size: 6535
AhdI GACnn_n'nnGTC
Cuts at: 5264 5264
Size: 6535
AleI CACnn'nnGTG
Cuts at: 1166 2033 1166
Size: 867 5668
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6535
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3754 3786 3754
Size: 32 6503
AlwNI CAG_nnn'CTG
Cuts at: 3095 4278 5850 3095
Size: 1183 1572 3780
Fragments arranged by size:
3780 1572 1183
ApaLI G'TGCA_C
Cuts at: 4592 5748 4592
Size: 1156 5379
ApoI r'AATT_y
Cuts at: 1493 2686 2695 3466 3844 4070 4222 6331 1493
Size: 1193 9 771 378 226 152 2109 1697
Fragments arranged by size:
2109 1697 1193 771 378 226 152 9
AseI AT'TA_AT
Cuts at: 160 1032 3075 5089 160
Size: 872 2043 2014 1606
Fragments arranged by size:
2043 2014 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6535
AvaI C'yCGr_G
Cuts at: 1870 1943 3860 1870
Size: 73 1917 4545
Fragments arranged by size:
4545 1917 73
BamHI G'GATC_C
Cuts at: 3865 4232 3865
Size: 367 6168
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2027 2222 2916 3856 5311
Size: 325 217 627 240 195 694 940 1455
Cuts at: 5311 618
Size: 1842
Fragments arranged by size:
1842 1455 940 694 627 325 240 217
195
BanII G_rGCy'C
Cuts at: 1391 2901 3187 3385 3854 1391
Size: 1510 286 198 469 4072
Fragments arranged by size:
4072 1510 469 286 198
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2302 928
Size: 971 403 5161
Fragments arranged by size:
5161 971 403
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4726 4760 1903
Size: 34 2789 34 3678
Fragments arranged by size:
3678 2789 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3606 4427 5637 3606
Size: 821 1210 4504
Fragments arranged by size:
4504 1210 821
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6535
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5146 136
Size: 107 122 71 631 605 3474 1525
Fragments arranged by size:
3474 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3103 6530 3103
Size: 3427 3108
BlpI GC'TnA_GC
Cuts at: 3952 3952
Size: 6535
Bme1580I G_kGCm'C
Cuts at: 1790 2172 2225 4596 5752 1790
Size: 382 53 2371 1156 2573
Fragments arranged by size:
2573 2371 1156 382 53
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6535
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2342 2549 4266
Size: 416 53 83 399 24 925 207 1717
Cuts at: 4266 5224 442
Size: 958 1753
Fragments arranged by size:
1753 1717 958 925 416 399 207 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2196 1056
Size: 1140 5395
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3052 5195 1239
Size: 1813 2143 2579
Fragments arranged by size:
2579 2143 1813
Bpu10I CC'TnA_GC
Cuts at: 3082 3082
Size: 6535
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4660 5525 5823 4660
Size: 865 298 5372
Fragments arranged by size:
5372 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3147 5198 882
Size: 617 1648 2051 2219
Fragments arranged by size:
2219 2051 1648 617
BsaAI yAC'GTr
Cuts at: 493 1204 2050 4326 493
Size: 711 846 2276 2702
Fragments arranged by size:
2702 2276 846 711
BsaBI GATnn'nnATC
Cuts at: 2605 6529 2605
Size: 3924 2611
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3212 4342 4724 5359
Size: 53 83 186 936 1679 1130 382 635
Cuts at: 5359 275
Size: 1451
Fragments arranged by size:
1679 1451 1130 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4968 5366 5640 5787 6198 4968
Size: 398 274 147 411 5305
Fragments arranged by size:
5305 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3318 3348 1178
Size: 30 165 30 1915 30 4365
Fragments arranged by size:
4365 1915 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6535
BseYI C'CCAG_C
Cuts at: 2108 5738 6234 2108
Size: 3630 496 2409
Fragments arranged by size:
3630 2409 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2506 2518 2816 2940 1163
Size: 573 770 12 298 124 4758
Fragments arranged by size:
4758 770 573 298 124 12
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2082 2152 3985 4746 5774 1540
Size: 275 186 81 70 1833 761 1028 2301
Fragments arranged by size:
2301 1833 1028 761 275 186 81 70
BsiHKAI G_wGCw'C
Cuts at: 3331 3385 4596 4681 5752 3331
Size: 54 1211 85 1071 4114
Fragments arranged by size:
4114 1211 1071 85 54
BsmI GAATG_Cn'
Cuts at: 4046 4139 4046
Size: 93 6442
BsmBI CGTCTCn'nnnn_
Cuts at: 2393 2393
Size: 6535
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2172 2225 2901 3187 3331 3385 3854
Size: 399 382 53 676 286 144 54 469
Cuts at: 3854 4596 4681 5752 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 676 469 399 382 286
144 85 54 53
BspEI T'CCGG_A
Cuts at: 6198 6198
Size: 6535
BspHI T'CATG_A
Cuts at: 4424 6154 4424
Size: 1730 4805
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3890 844
Size: 3046 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4422 1394
Size: 429 2599 3507
Fragments arranged by size:
3507 2599 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3413 3419 3826 5030 5204 65
Size: 1183 2165 6 407 1204 174 1396
Fragments arranged by size:
2165 1396 1204 1183 407 174 6
BsrFI r'CCGG_y
Cuts at: 1700 1810 5179 5366 1700
Size: 110 3369 187 2869
Fragments arranged by size:
3369 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6535
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6535
BssSI C'ACGA_G
Cuts at: 4595 5607 4595
Size: 1012 5523
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6535
BstBI TT'CG_AA
Cuts at: 5339 5339
Size: 6535
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6535
BstYI r'GATC_y
Cuts at: 1972 3103 3865 4232 4617 4634 6086 6172 6184
Size: 1131 762 367 385 17 1452 86 12
Cuts at: 6184 6530 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1131 762 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2430 2430
Size: 6535
BtgI C'CryG_G
Cuts at: 513 1065 2004 2015 2172 513
Size: 552 939 11 157 4876
Fragments arranged by size:
4876 939 552 157 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5796
Fragments arranged by size:
5796 683 56
BtsI GCAGTG_nn'
Cuts at: 2172 4051 4845 4865 2172
Size: 1879 794 20 3842
Fragments arranged by size:
3842 1879 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6535
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2496 2531 529
Size: 35 1932 35 4533
Fragments arranged by size:
4533 1932 35 35
DraI TTT'AAA
Cuts at: 2658 3839 2658
Size: 1181 5354
DraIII CAC_nnn'GTG
Cuts at: 2543 2543
Size: 6535
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5542 809
Size: 507 4226 1802
Fragments arranged by size:
4226 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2079 2116 2149
Size: 54 1425 50 275 18 249 37 33
Cuts at: 2149 3982 4871 6500 8
Size: 1833 889 1629 43
Fragments arranged by size:
1833 1629 1425 889 275 249 54 50
43 37 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 2079 2149 3982 1537
Size: 275 267 70 1833 4090
Fragments arranged by size:
4090 1833 275 267 70
EarI CTCTTCn'nnn_
Cuts at: 1353 2411 4465 1353
Size: 1058 2054 3423
Fragments arranged by size:
3423 2054 1058
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5122 5496 5642 387
Size: 1064 138 3533 374 146 1280
Fragments arranged by size:
3533 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3383 3383
Size: 6535
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3052 3106 3559 4598 5195 5982 6250
Size: 393 1420 54 453 1039 597 787 268
Cuts at: 6250 1239
Size: 1524
Fragments arranged by size:
1524 1420 1039 787 597 453 393 268
54
EcoO109I rG'GnC_Cy
Cuts at: 2751 2751
Size: 6535
EcoRI G'AATT_C
Cuts at: 3844 4222 3844
Size: 378 6157
EcoRV GAT'ATC
Cuts at: 3433 3433
Size: 6535
FspI TGC'GCA
Cuts at: 5041 5041
Size: 6535
HaeII r_GCGC'y
Cuts at: 1678 2180 5682 1678
Size: 502 3502 2531
Fragments arranged by size:
3502 2531 502
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3674 3706 5176 5208 5250
Size: 32 135 32 2102 32 1470 32 42
Cuts at: 5250 5282 1373
Size: 32 2626
Fragments arranged by size:
2626 2102 1470 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2782 3879 4125 669
Size: 988 281 844 1097 246 3079
Fragments arranged by size:
3079 1097 988 844 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6535
HpaI GTT'AAC
Cuts at: 4125 4125
Size: 6535
KpnI G_GTAC'C
Cuts at: 2920 3860 2920
Size: 940 5595
MfeI C'AATT_G
Cuts at: 3321 4134 3321
Size: 813 5722
MluI A'CGCG_T
Cuts at: 6411 6411
Size: 6535
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3352 3565 5649 5833 1305
Size: 2047 213 2084 184 2007
Fragments arranged by size:
2084 2047 2007 213 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4713
Fragments arranged by size:
4713 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2033 2925 3485 4493 4852 5011 518
Size: 648 867 892 560 1008 359 159 2042
Fragments arranged by size:
2042 1008 892 867 648 560 359 159
MspA1I CmG'CkG
Cuts at: 1606 3951 4628 5776 6021 1606
Size: 2345 677 1148 245 2120
Fragments arranged by size:
2345 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5044
Fragments arranged by size:
5044 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6535
NheI G'CTAG_C
Cuts at: 1052 2192 1052
Size: 1140 5395
NotI GC'GGCC_GC
Cuts at: 2149 3982 2149
Size: 1833 4702
NspI r_CATG'y
Cuts at: 1132 1229 2068 2883 3725 3893 5438 1132
Size: 97 839 815 842 168 1545 2229
Fragments arranged by size:
2229 1545 842 839 815 168 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6535
PciI A'CATG_T
Cuts at: 5434 5434
Size: 6535
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6535
PfoI T'CCnGG_A
Cuts at: 3007 3016 3007
Size: 9 6526
PmeI GTTT'AAAC
Cuts at: 3839 3839
Size: 6535
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4604 4636 6117 6149 4604
Size: 32 1481 32 4990
Fragments arranged by size:
4990 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2751 2751
Size: 6535
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6535
PsiI TTA'TAA
Cuts at: 4105 4105
Size: 6535
PstI C_TGCA'G
Cuts at: 830 3887 830
Size: 3057 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6535
SacI G_AGCT'C
Cuts at: 3385 3385
Size: 6535
SalI G'TCGA_C
Cuts at: 1936 3877 1936
Size: 1941 4594
SbfI CC_TGCA'GG
Cuts at: 3887 3887
Size: 6535
ScaI AGT'ACT
Cuts at: 4783 6495 4783
Size: 1712 4823
SfcI C'TryA_G
Cuts at: 826 1045 3883 5018 5699 5890 826
Size: 219 2838 1135 681 191 1471
Fragments arranged by size:
2838 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6535
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6535
SmaI CCC'GGG
Cuts at: 1872 3862 1872
Size: 1990 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4639 5540 5802 820
Size: 197 926 2696 901 262 1553
Fragments arranged by size:
2696 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4326 493
Size: 3833 2702
SpeI A'CTAG_T
Cuts at: 152 3500 152
Size: 3348 3187
SphI G_CATG'C
Cuts at: 1132 2068 3893 1132
Size: 936 1825 3774
Fragments arranged by size:
3774 1825 936
SspI AAT'ATT
Cuts at: 5 52 4459 5
Size: 47 4407 2081
Fragments arranged by size:
4407 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6535
StyI C'CwwG_G
Cuts at: 513 1065 2004 2130 2933 3160 3436 3974 513
Size: 552 939 126 803 227 276 538 3074
Fragments arranged by size:
3074 939 803 552 538 276 227 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2531 4562 4579 4732 4917 5362 325
Size: 1255 951 2031 17 153 185 445 1498
Fragments arranged by size:
2031 1498 1255 951 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3570 4781 6493
Size: 274 80 33 51 1443 1593 1211 1712
Cuts at: 6493 96
Size: 138
Fragments arranged by size:
1712 1593 1443 1211 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3012 3044 3156 3188 6495 6527 3012
Size: 32 112 32 3307 32 3020
Fragments arranged by size:
3307 3020 112 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6535
XbaI T'CTAG_A
Cuts at: 3871 3871
Size: 6535
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6535
XmaI C'CCGG_G
Cuts at: 1870 3860 1870
Size: 1990 4545
XmnI GAAnn'nnTTC
Cuts at: 4664 4664
Size: 6535
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4343 276
Size: 53 83 186 936 2809 2468
Fragments arranged by size:
2809 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstXI Bsu36I EcoNI FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI