Restriction map for
pFN21AE6488
(Circular) (Six-base) MAPSORT of: pFN21AE6488.seq Check: 7445 from: 1 to: 6382
pFN21AE6488 6382 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2187 3119 2187
Size: 932 5450
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4192 278
Size: 53 83 186 936 2656 2468
Fragments arranged by size:
2656 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3725 1937
Size: 1788 4594
Acc65I G'GTAC_C
Cuts at: 3703 3703
Size: 6382
AclI AA'CG_TT
Cuts at: 1563 2453 4509 4882 1563
Size: 890 2056 373 3063
Fragments arranged by size:
3063 2056 890 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2144 3467 3671 4445 5829 6097 1632
Size: 512 1323 204 774 1384 268 1917
Fragments arranged by size:
1917 1384 1323 774 512 268 204
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6185
AflIII A'CryG_T
Cuts at: 1203 2478 5281 6258 1203
Size: 1275 2803 977 1327
Fragments arranged by size:
2803 1327 1275 977
AgeI A'CCGG_T
Cuts at: 5213 5213
Size: 6382
AhdI GACnn_n'nnGTC
Cuts at: 5111 5111
Size: 6382
AleI CACnn'nnGTG
Cuts at: 1166 2060 2762 1166
Size: 894 702 4786
Fragments arranged by size:
4786 894 702
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2078 1184
Size: 894 5488
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2480 2512 2480
Size: 32 6350
AlwNI CAG_nnn'CTG
Cuts at: 2340 4125 5697 2340
Size: 1785 1572 3025
Fragments arranged by size:
3025 1785 1572
ApaI G_GGCC'C
Cuts at: 3647 3647
Size: 6382
ApaLI G'TGCA_C
Cuts at: 4439 5595 4439
Size: 1156 5226
ApoI r'AATT_y
Cuts at: 1493 3691 3917 4069 6178 1493
Size: 2198 226 152 2109 1697
Fragments arranged by size:
2198 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4936 160
Size: 872 3904 1606
Fragments arranged by size:
3904 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6382
AvaI C'yCGr_G
Cuts at: 1870 1943 2379 3707 1870
Size: 73 436 1328 4545
Fragments arranged by size:
4545 1328 436 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2012 2045 2012
Size: 33 6349
BamHI G'GATC_C
Cuts at: 2289 3712 4079 2289
Size: 1423 367 4592
Fragments arranged by size:
4592 1423 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2852 3703 5158 618
Size: 325 217 627 1065 851 1455 1842
Fragments arranged by size:
1842 1455 1065 851 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2108 3531 3647 3701 1391
Size: 717 1423 116 54 4072
Fragments arranged by size:
4072 1423 717 116 54
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3070 928
Size: 971 1171 4240
Fragments arranged by size:
4240 1171 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3166 3200 4573 4607 1903
Size: 34 1229 34 1373 34 3678
Fragments arranged by size:
3678 1373 1229 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4274 5484 4274
Size: 1210 5172
BclI T'GATC_A
Cuts at: 1548 2115 1548
Size: 567 5815
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2057 2582 4993
Size: 107 122 71 631 605 385 525 2411
Cuts at: 4993 136
Size: 1525
Fragments arranged by size:
2411 1525 631 605 525 385 122 107
71
BglII A'GATC_T
Cuts at: 6377 6377
Size: 6382
BlpI GC'TnA_GC
Cuts at: 2264 2786 3659 3799 2264
Size: 522 873 140 4847
Fragments arranged by size:
4847 873 522 140
Bme1580I G_kGCm'C
Cuts at: 1790 2156 2855 3179 3610 3647 4443 5599 1790
Size: 366 699 324 431 37 796 1156 2573
Fragments arranged by size:
2573 1156 796 699 431 366 324 37
BmgBI CAC'GTC
Cuts at: 1318 2386 1318
Size: 1068 5314
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2294 4113 5071
Size: 416 53 83 399 24 877 1819 958
Cuts at: 5071 442
Size: 1753
Fragments arranged by size:
1819 1753 958 877 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6382
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2087 2828 3011 3320 3599 5042 1239
Size: 848 741 183 309 279 1443 2579
Fragments arranged by size:
2579 1443 848 741 309 279 183
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4507 5372 5670 4507
Size: 865 298 5219
Fragments arranged by size:
5219 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5045 882
Size: 617 3546 2219
Fragments arranged by size:
3546 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2151 4173 493
Size: 711 947 2022 2702
Fragments arranged by size:
2702 2022 947 711
BsaBI GATnn'nnATC
Cuts at: 6376 6376
Size: 6382
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3162 4189 4571 5206
Size: 53 83 186 936 1629 1027 382 635
Cuts at: 5206 275
Size: 1451
Fragments arranged by size:
1629 1451 1027 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2031 4815 5213 5487 5634 6045 2031
Size: 2784 398 274 147 411 2368
Fragments arranged by size:
2784 2368 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2480 2510 1178
Size: 30 165 30 1077 30 5050
Fragments arranged by size:
5050 1077 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2918 3061 1186
Size: 1732 143 4507
Fragments arranged by size:
4507 1732 143
BseYI C'CCAG_C
Cuts at: 2100 2155 2801 2946 5585 6081 2100
Size: 55 646 145 2639 496 2401
Fragments arranged by size:
2639 2401 646 496 145 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2038 2341 2626 3341 1163
Size: 573 302 303 285 715 4204
Fragments arranged by size:
4204 715 573 303 302 285
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3832 4593 5621 1540
Size: 275 186 1831 761 1028 2301
Fragments arranged by size:
2301 1831 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2265 4443 4528 5599 2265
Size: 2178 85 1071 3048
Fragments arranged by size:
3048 2178 1071 85
BsmI GAATG_Cn'
Cuts at: 3893 3986 3893
Size: 93 6289
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2108 2156 2265 2855 3179 3531 3610
Size: 399 318 48 109 590 324 352 79
Cuts at: 3610 3647 3701 4443 4528 5599 1391
Size: 37 54 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 590 399 352 324 318
109 85 79 54 48 37
BspEI T'CCGG_A
Cuts at: 6045 6045
Size: 6382
BspHI T'CATG_A
Cuts at: 2514 4271 6001 2514
Size: 1757 1730 2895
Fragments arranged by size:
2895 1757 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2187 2499 3119 3294 3737 844
Size: 1343 312 620 175 443 3489
Fragments arranged by size:
3489 1343 620 443 312 175
BsrBI CCG'CTC
Cuts at: 1394 1823 4269 1394
Size: 429 2446 3507
Fragments arranged by size:
3507 2446 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4877 5051 65
Size: 1183 3629 174 1396
Fragments arranged by size:
3629 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2576 5026 5213 1700
Size: 110 766 2450 187 2869
Fragments arranged by size:
2869 2450 766 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6382
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6382
BssSI C'ACGA_G
Cuts at: 4442 5454 4442
Size: 1012 5370
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2340 794
Size: 1546 4836
BstBI TT'CG_AA
Cuts at: 5186 5186
Size: 6382
BstEII G'GTnAC_C
Cuts at: 1184 2744 3673 1184
Size: 1560 929 3893
Fragments arranged by size:
3893 1560 929
BstXI CCAn_nnnn'nTGG
Cuts at: 2506 3640 2506
Size: 1134 5248
BstYI r'GATC_y
Cuts at: 1972 2289 3240 3712 4079 4464 4481 5933 6019
Size: 317 951 472 367 385 17 1452 86
Cuts at: 6019 6031 6377 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 951 472 385 367 346 317
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2679 2679
Size: 6382
BtgI C'CryG_G
Cuts at: 513 1065 2004 2772 2934 513
Size: 552 939 768 162 3961
Fragments arranged by size:
3961 939 768 552 162
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3518 487
Size: 683 56 2292 3351
Fragments arranged by size:
3351 2292 683 56
BtsI GCAGTG_nn'
Cuts at: 2341 2346 3417 3898 4692 4712 2341
Size: 5 1071 481 794 20 4011
Fragments arranged by size:
4011 1071 794 481 20 5
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6382
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2695 2730 529
Size: 35 2131 35 4181
Fragments arranged by size:
4181 2131 35 35
DraI TTT'AAA
Cuts at: 3686 3686
Size: 6382
DraIII CAC_nnn'GTG
Cuts at: 2737 2737
Size: 6382
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5389 809
Size: 507 4073 1802
Fragments arranged by size:
4073 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2201 3010 3829
Size: 54 1425 50 275 18 371 809 819
Cuts at: 3829 4718 6347 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 819 809 371 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3829 1537
Size: 275 2017 4090
Fragments arranged by size:
4090 2017 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2081 2539 2756 3599 4312 1353
Size: 728 458 217 843 713 3423
Fragments arranged by size:
3423 843 728 713 458 217
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3157 4969 5343 5489 387
Size: 1064 138 1568 1812 374 146 1280
Fragments arranged by size:
1812 1568 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2087 2144 2828 3011 3320 3467 3599
Size: 393 455 57 684 183 309 147 132
Cuts at: 3599 3671 4445 5042 5829 6097 1239
Size: 72 774 597 787 268 1524
Fragments arranged by size:
1524 787 774 684 597 455 393 309
268 183 147 132 72 57
EcoO109I rG'GnC_Cy
Cuts at: 2797 3512 2797
Size: 715 5667
EcoRI G'AATT_C
Cuts at: 3691 4069 3691
Size: 378 6004
FspI TGC'GCA
Cuts at: 4888 4888
Size: 6382
HaeII r_GCGC'y
Cuts at: 1678 2041 2271 2420 5529 1678
Size: 363 230 149 3109 2531
Fragments arranged by size:
3109 2531 363 230 149
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5023 5055 5097 5129 1373
Size: 32 135 32 3451 32 42 32 2626
Fragments arranged by size:
3451 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3726 3972 669
Size: 988 281 1788 246 3079
Fragments arranged by size:
3079 1788 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6382
HpaI GTT'AAC
Cuts at: 3972 3972
Size: 6382
KpnI G_GTAC'C
Cuts at: 3707 3707
Size: 6382
MfeI C'AATT_G
Cuts at: 3981 3981
Size: 6382
MluI A'CGCG_T
Cuts at: 6258 6258
Size: 6382
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3447 5496 5680 1305
Size: 2142 2049 184 2007
Fragments arranged by size:
2142 2049 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2203 3012 10
Size: 54 1425 343 371 809 3380
Fragments arranged by size:
3380 1425 809 371 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2060 2363 2504 2762 2939 3503 4340
Size: 648 894 303 141 258 177 564 837
Cuts at: 4340 4699 4858 518
Size: 359 159 2042
Fragments arranged by size:
2042 894 837 648 564 359 303 258
177 159 141
MspA1I CmG'CkG
Cuts at: 1606 2159 2950 3094 3798 4475 5623 5868 1606
Size: 553 791 144 704 677 1148 245 2120
Fragments arranged by size:
2120 1148 791 704 677 553 245 144
NcoI C'CATG_G
Cuts at: 513 1065 2004 2772 2934 513
Size: 552 939 768 162 3961
Fragments arranged by size:
3961 939 768 552 162
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6382
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6382
NotI GC'GGCC_GC
Cuts at: 3829 3829
Size: 6382
NspI r_CATG'y
Cuts at: 1132 1229 2482 3740 5285 1132
Size: 97 1253 1258 1545 2229
Fragments arranged by size:
2229 1545 1258 1253 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6382
PciI A'CATG_T
Cuts at: 2478 5281 2478
Size: 2803 3579
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6382
PmeI GTTT'AAAC
Cuts at: 3686 3686
Size: 6382
PmlI CAC'GTG
Cuts at: 2151 2151
Size: 6382
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2480 2512 4451 4483 5964 5996 2480
Size: 32 1939 32 1481 32 2866
Fragments arranged by size:
2866 1939 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3512 3512
Size: 6382
PshAI GACnn'nnGTC
Cuts at: 1613 3350 1613
Size: 1737 4645
PsiI TTA'TAA
Cuts at: 3952 3952
Size: 6382
PspOMI G'GGCC_C
Cuts at: 3643 3643
Size: 6382
PstI C_TGCA'G
Cuts at: 830 3412 3453 3734 830
Size: 2582 41 281 3478
Fragments arranged by size:
3478 2582 281 41
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6382
PvuII CAG'CTG
Cuts at: 2159 2950 3094 2159
Size: 791 144 5447
Fragments arranged by size:
5447 791 144
SalI G'TCGA_C
Cuts at: 1936 3724 1936
Size: 1788 4594
SanDI GG'GwC_CC
Cuts at: 3512 3512
Size: 6382
SbfI CC_TGCA'GG
Cuts at: 3734 3734
Size: 6382
ScaI AGT'ACT
Cuts at: 4630 6342 4630
Size: 1712 4670
SexAI A'CCwGG_T
Cuts at: 2606 2606
Size: 6382
SfcI C'TryA_G
Cuts at: 826 1045 3408 3449 3730 4865 5546 5737 826
Size: 219 2363 41 281 1135 681 191 1471
Fragments arranged by size:
2363 1471 1135 681 281 219 191 41
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6382
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6382
SmaI CCC'GGG
Cuts at: 1872 2381 3709 1872
Size: 509 1328 4545
Fragments arranged by size:
4545 1328 509
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4486 5387 5649 820
Size: 197 926 2543 901 262 1553
Fragments arranged by size:
2543 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4173 493
Size: 3680 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6382
SphI G_CATG'C
Cuts at: 1132 3740 1132
Size: 2608 3774
SspI AAT'ATT
Cuts at: 5 52 4306 5
Size: 47 4254 2081
Fragments arranged by size:
4254 2081 47
StuI AGG'CCT
Cuts at: 1517 3447 1517
Size: 1930 4452
StyI C'CwwG_G
Cuts at: 513 1065 2004 2772 2934 3821 513
Size: 552 939 768 162 887 3074
Fragments arranged by size:
3074 939 887 768 552 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2195 2774 3526 4409 4426 4579 4764
Size: 1255 615 579 752 883 17 153 185
Cuts at: 4764 5209 325
Size: 445 1498
Fragments arranged by size:
1498 1255 883 752 615 579 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2089 4628 6340
Size: 274 80 33 51 1443 112 2539 1712
Cuts at: 6340 96
Size: 138
Fragments arranged by size:
2539 1712 1443 274 138 112 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2274 2306 6342 6374 2274
Size: 32 4036 32 2282
Fragments arranged by size:
4036 2282 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6382
XbaI T'CTAG_A
Cuts at: 3718 3718
Size: 6382
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2281 3389 2281
Size: 1108 5274
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6382
XmaI C'CCGG_G
Cuts at: 1870 2379 3707 1870
Size: 509 1328 4545
Fragments arranged by size:
4545 1328 509
XmnI GAAnn'nnTTC
Cuts at: 2075 2667 2865 4511 2075
Size: 592 198 1646 3946
Fragments arranged by size:
3946 1646 592 198
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4190 276
Size: 53 83 186 936 2656 2468
Fragments arranged by size:
2656 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PspXI PsrI RsrII SacI SacII SapI SfiI
SfoI SrfI SwaI