Restriction map for
pFN21AE6485
(Circular) (Six-base) MAPSORT of: pFN21AE6485.seq Check: 6567 from: 1 to: 6571
pFN21AE6485 6571 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4381 278
Size: 53 83 186 936 2845 2468
Fragments arranged by size:
2845 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3039 3914 1937
Size: 1102 875 4594
Fragments arranged by size:
4594 1102 875
Acc65I G'GTAC_C
Cuts at: 3892 3892
Size: 6571
AclI AA'CG_TT
Cuts at: 1563 4698 5071 1563
Size: 3135 373 3063
Fragments arranged by size:
3135 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3240 3259 4634 6018 6286 1632
Size: 1608 19 1375 1384 268 1917
Fragments arranged by size:
1917 1608 1384 1375 268 19
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6374
AflIII A'CryG_T
Cuts at: 1203 5470 6447 1203
Size: 4267 977 1327
Fragments arranged by size:
4267 1327 977
AgeI A'CCGG_T
Cuts at: 5402 5402
Size: 6571
AhdI GACnn_n'nnGTC
Cuts at: 5300 5300
Size: 6571
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6571
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6571
AlwNI CAG_nnn'CTG
Cuts at: 3260 3754 4314 5886 3260
Size: 494 560 1572 3945
Fragments arranged by size:
3945 1572 560 494
ApaLI G'TGCA_C
Cuts at: 2088 4628 5784 2088
Size: 2540 1156 2875
Fragments arranged by size:
2875 2540 1156
ApoI r'AATT_y
Cuts at: 1493 2184 2350 3880 4106 4258 6367 1493
Size: 691 166 1530 226 152 2109 1697
Fragments arranged by size:
2109 1697 1530 691 226 166 152
AseI AT'TA_AT
Cuts at: 160 1032 5125 160
Size: 872 4093 1606
Fragments arranged by size:
4093 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6571
AvaI C'yCGr_G
Cuts at: 1870 1943 3896 1870
Size: 73 1953 4545
Fragments arranged by size:
4545 1953 73
BamHI G'GATC_C
Cuts at: 3901 4268 3901
Size: 367 6204
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3892 5347 618
Size: 325 217 627 2105 1455 1842
Fragments arranged by size:
2105 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2027 2880 3127 3890 1391
Size: 636 853 247 763 4072
Fragments arranged by size:
4072 853 763 636 247
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2963 3728 928
Size: 971 1064 765 3771
Fragments arranged by size:
3771 1064 971 765
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4762 4796 1903
Size: 34 2825 34 3678
Fragments arranged by size:
3678 2825 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2918 4463 5673 2918
Size: 1545 1210 3816
Fragments arranged by size:
3816 1545 1210
BclI T'GATC_A
Cuts at: 1548 3361 1548
Size: 1813 4758
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5182 136
Size: 107 122 71 631 605 3510 1525
Fragments arranged by size:
3510 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6566 6566
Size: 6571
BlpI GC'TnA_GC
Cuts at: 3988 3988
Size: 6571
Bme1580I G_kGCm'C
Cuts at: 1790 2092 4632 5788 1790
Size: 302 2540 1156 2573
Fragments arranged by size:
2573 2540 1156 302
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6571
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2377 2680 4302
Size: 416 53 83 399 24 960 303 1622
Cuts at: 4302 5260 442
Size: 958 1753
Fragments arranged by size:
1753 1622 960 958 416 399 303 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3584 1056
Size: 2528 4043
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2046 2396 5231 1239
Size: 807 350 2835 2579
Fragments arranged by size:
2835 2579 807 350
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4696 5561 5859 4696
Size: 865 298 5408
Fragments arranged by size:
5408 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5234 882
Size: 617 3735 2219
Fragments arranged by size:
3735 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4362 493
Size: 711 3158 2702
Fragments arranged by size:
3158 2702 711
BsaBI GATnn'nnATC
Cuts at: 6565 6565
Size: 6571
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4378 4760 5395 275
Size: 53 83 186 936 2845 382 635 1451
Fragments arranged by size:
2845 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5004 5402 5676 5823 6234 5004
Size: 398 274 147 411 5341
Fragments arranged by size:
5341 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6346
Fragments arranged by size:
6346 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2349 1186
Size: 1163 5408
BseYI C'CCAG_C
Cuts at: 2020 3841 5774 6270 2020
Size: 1821 1933 496 2321
Fragments arranged by size:
2321 1933 1821 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2053 2442 1163
Size: 573 317 389 5292
Fragments arranged by size:
5292 573 389 317
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4021 4782 5810 1540
Size: 275 186 2020 761 1028 2301
Fragments arranged by size:
2301 2020 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2092 2880 4632 4717 5788 2092
Size: 788 1752 85 1071 2875
Fragments arranged by size:
2875 1752 1071 788 85
BsmI GAATG_Cn'
Cuts at: 4082 4175 4082
Size: 93 6478
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2027 2092 2880 3127 3890 4632 4717
Size: 399 237 65 788 247 763 742 85
Cuts at: 4717 5788 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 788 763 742 399 247 237
85 65
BspEI T'CCGG_A
Cuts at: 6234 6234
Size: 6571
BspHI T'CATG_A
Cuts at: 4460 6190 4460
Size: 1730 4841
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3926 844
Size: 3082 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4458 1394
Size: 429 2635 3507
Fragments arranged by size:
3507 2635 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5066 5240 65
Size: 1183 3818 174 1396
Fragments arranged by size:
3818 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5215 5402 1700
Size: 110 3405 187 2869
Fragments arranged by size:
3405 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3287 96
Size: 3191 3380
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6571
BssSI C'ACGA_G
Cuts at: 4631 5643 4631
Size: 1012 5559
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6571
BstBI TT'CG_AA
Cuts at: 5375 5375
Size: 6571
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6571
BstXI CCAn_nnnn'nTGG
Cuts at: 2670 2670
Size: 6571
BstYI r'GATC_y
Cuts at: 1972 2290 3467 3901 4268 4653 4670 6122 6208
Size: 318 1177 434 367 385 17 1452 86
Cuts at: 6208 6220 6566 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1177 434 385 367 346 318
86 17 12
BstZ17I GTA'TAC
Cuts at: 3040 3040
Size: 6571
Bsu36I CC'TnA_GG
Cuts at: 3716 3716
Size: 6571
BtgI C'CryG_G
Cuts at: 513 1065 2004 2047 3441 3828 513
Size: 552 939 43 1394 387 3256
Fragments arranged by size:
3256 1394 939 552 387 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5832
Fragments arranged by size:
5832 683 56
BtsI GCAGTG_nn'
Cuts at: 2036 3325 3367 4087 4881 4901 2036
Size: 1289 42 720 794 20 3706
Fragments arranged by size:
3706 1289 794 720 42 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6571
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2419 2454 529
Size: 35 1855 35 4646
Fragments arranged by size:
4646 1855 35 35
DraI TTT'AAA
Cuts at: 2349 2613 2961 3875 2349
Size: 264 348 914 5045
Fragments arranged by size:
5045 914 348 264
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5578 809
Size: 507 4262 1802
Fragments arranged by size:
4262 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4018 4907 6536
Size: 54 1425 50 275 18 2188 889 1629
Cuts at: 6536 8
Size: 43
Fragments arranged by size:
2188 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4018 1537
Size: 275 2206 4090
Fragments arranged by size:
4090 2206 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2133 2367 2885 4501 1353
Size: 780 234 518 1616 3423
Fragments arranged by size:
3423 1616 780 518 234
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5158 5532 5678 387
Size: 1064 138 3569 374 146 1280
Fragments arranged by size:
3569 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2878 2878
Size: 6571
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2046 2396 3240 3259 4634 5231 6018
Size: 393 414 350 844 19 1375 597 787
Cuts at: 6018 6286 1239
Size: 268 1524
Fragments arranged by size:
1524 1375 844 787 597 414 393 350
268 19
EcoRI G'AATT_C
Cuts at: 3880 4258 3880
Size: 378 6193
EcoRV GAT'ATC
Cuts at: 2737 2737
Size: 6571
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2987 2987
Size: 6571
FspI TGC'GCA
Cuts at: 5077 5077
Size: 6571
HaeII r_GCGC'y
Cuts at: 1678 5718 1678
Size: 4040 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2067 2099 2211 2243 3459
Size: 32 135 32 495 32 112 32 1216
Cuts at: 3459 3491 3800 3832 5212 5244 5286 5318 1373
Size: 32 309 32 1380 32 42 32 2626
Fragments arranged by size:
2626 1380 1216 495 309 135 112 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3606 3915 4161 669
Size: 988 281 1668 309 246 3079
Fragments arranged by size:
3079 1668 988 309 281 246
HindIII A'AGCT_T
Cuts at: 748 2708 748
Size: 1960 4611
HpaI GTT'AAC
Cuts at: 4161 4161
Size: 6571
KpnI G_GTAC'C
Cuts at: 3896 3896
Size: 6571
MfeI C'AATT_G
Cuts at: 4170 4170
Size: 6571
MluI A'CGCG_T
Cuts at: 6447 6447
Size: 6571
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2401 5685 5869 1305
Size: 1096 3284 184 2007
Fragments arranged by size:
3284 2007 1096 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4749
Fragments arranged by size:
4749 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2046 3330 4529 4888 5047 518
Size: 648 880 1284 1199 359 159 2042
Fragments arranged by size:
2042 1284 1199 880 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3987 4664 5812 6057 1606
Size: 2381 677 1148 245 2120
Fragments arranged by size:
2381 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2047 3441 3828 513
Size: 552 939 43 1394 387 3256
Fragments arranged by size:
3256 1394 939 552 387 43
NdeI CA'TA_TG
Cuts at: 387 2805 3696 387
Size: 2418 891 3262
Fragments arranged by size:
3262 2418 891
NheI G'CTAG_C
Cuts at: 1052 3580 1052
Size: 2528 4043
NotI GC'GGCC_GC
Cuts at: 4018 4018
Size: 6571
NspI r_CATG'y
Cuts at: 1132 1229 2491 3929 5474 1132
Size: 97 1262 1438 1545 2229
Fragments arranged by size:
2229 1545 1438 1262 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6571
PciI A'CATG_T
Cuts at: 5470 5470
Size: 6571
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2069 1108
Size: 961 5610
PfoI T'CCnGG_A
Cuts at: 2062 2062
Size: 6571
PmeI GTTT'AAAC
Cuts at: 3875 3875
Size: 6571
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4640 4672 6153 6185 4640
Size: 32 1481 32 5026
Fragments arranged by size:
5026 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6571
PsiI TTA'TAA
Cuts at: 3302 4141 3302
Size: 839 5732
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3023 3055 3271 3303 3023
Size: 32 216 32 6291
Fragments arranged by size:
6291 216 32 32
PstI C_TGCA'G
Cuts at: 830 2868 3681 3923 830
Size: 2038 813 242 3478
Fragments arranged by size:
3478 2038 813 242
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6571
SacI G_AGCT'C
Cuts at: 2880 2880
Size: 6571
SalI G'TCGA_C
Cuts at: 1936 3913 1936
Size: 1977 4594
SapI GCTCTTCn'nnn_
Cuts at: 2133 2885 2133
Size: 752 5819
SbfI CC_TGCA'GG
Cuts at: 3923 3923
Size: 6571
ScaI AGT'ACT
Cuts at: 4819 6531 4819
Size: 1712 4859
SfcI C'TryA_G
Cuts at: 826 1045 2864 3677 3919 5054 5735 5926 826
Size: 219 1819 813 242 1135 681 191 1471
Fragments arranged by size:
1819 1471 1135 813 681 242 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6571
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6571
SmaI CCC'GGG
Cuts at: 1872 3898 1872
Size: 2026 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4675 5576 5838 820
Size: 197 926 2732 901 262 1553
Fragments arranged by size:
2732 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4362 493
Size: 3869 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6571
SphI G_CATG'C
Cuts at: 1132 3929 1132
Size: 2797 3774
SspI AAT'ATT
Cuts at: 5 52 4495 5
Size: 47 4443 2081
Fragments arranged by size:
4443 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6571
StyI C'CwwG_G
Cuts at: 513 1065 2004 2047 3008 3441 3557 3828 4010
Size: 552 939 43 961 433 116 271 182
Cuts at: 4010 513
Size: 3074
Fragments arranged by size:
3074 961 939 552 433 271 182 116
43
SwaI ATTT'AAAT
Cuts at: 2349 2349
Size: 6571
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2327 3855 4598 4615 4768 4953 5398
Size: 1255 747 1528 743 17 153 185 445
Cuts at: 5398 325
Size: 1498
Fragments arranged by size:
1528 1498 1255 747 743 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3287 3822 4817
Size: 274 80 33 51 1443 1310 535 995
Cuts at: 4817 6529 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1310 995 535 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2511 2543 6531 6563 2511
Size: 32 3988 32 2519
Fragments arranged by size:
3988 2519 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2162 3709 1316
Size: 846 1547 4178
Fragments arranged by size:
4178 1547 846
XbaI T'CTAG_A
Cuts at: 3907 3907
Size: 6571
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6571
XmaI C'CCGG_G
Cuts at: 1870 3896 1870
Size: 2026 4545
XmnI GAAnn'nnTTC
Cuts at: 2127 4700 2127
Size: 2573 3998
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4379 276
Size: 53 83 186 936 2845 2468
Fragments arranged by size:
2845 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PsrI PstI PvuI SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI DraIII EcoNI
EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PvuII RsrII
SacII SanDI SexAI SfiI SfoI SrfI XcmI