Restriction map for
pFN21AE6484
(Circular) (Six-base) MAPSORT of: pFN21AE6484.seq Check: 7732 from: 1 to: 6568
pFN21AE6484 6568 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4378 278
Size: 53 83 186 936 2842 2468
Fragments arranged by size:
2842 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2664 3320 3911 1937
Size: 727 656 591 4594
Fragments arranged by size:
4594 727 656 591
Acc65I G'GTAC_C
Cuts at: 3202 3889 3202
Size: 687 5881
AclI AA'CG_TT
Cuts at: 1563 4695 5068 1563
Size: 3132 373 3063
Fragments arranged by size:
3132 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2436 3314 4631 6015 6283 1632
Size: 804 878 1317 1384 268 1917
Fragments arranged by size:
1917 1384 1317 878 804 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6371
AflIII A'CryG_T
Cuts at: 1203 2980 5467 6444 1203
Size: 1777 2487 977 1327
Fragments arranged by size:
2487 1777 1327 977
AgeI A'CCGG_T
Cuts at: 5399 5399
Size: 6568
AhdI GACnn_n'nnGTC
Cuts at: 5297 5297
Size: 6568
AleI CACnn'nnGTG
Cuts at: 1166 3812 1166
Size: 2646 3922
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6568
AlwNI CAG_nnn'CTG
Cuts at: 4311 5883 4311
Size: 1572 4996
ApaLI G'TGCA_C
Cuts at: 2115 4625 5781 2115
Size: 2510 1156 2902
Fragments arranged by size:
2902 2510 1156
ApoI r'AATT_y
Cuts at: 1493 2087 3503 3877 4103 4255 6364 1493
Size: 594 1416 374 226 152 2109 1697
Fragments arranged by size:
2109 1697 1416 594 374 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5122 160
Size: 872 4090 1606
Fragments arranged by size:
4090 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6568
AvaI C'yCGr_G
Cuts at: 1870 1943 2451 3893 1870
Size: 73 508 1442 4545
Fragments arranged by size:
4545 1442 508 73
BamHI G'GATC_C
Cuts at: 3898 4265 3898
Size: 367 6201
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2200 2826 3202 3741 3889
Size: 325 217 627 413 626 376 539 148
Cuts at: 3889 5344 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 626 539 413 376 325
217 148
BanII G_rGCy'C
Cuts at: 1391 2261 2436 3887 1391
Size: 870 175 1451 4072
Fragments arranged by size:
4072 1451 870 175
BbeI G_GCGC'C
Cuts at: 2830 2830
Size: 6568
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2034 2201 3184 928
Size: 971 135 167 983 4312
Fragments arranged by size:
4312 983 971 167 135
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4759 4793 1903
Size: 34 2822 34 3678
Fragments arranged by size:
3678 2822 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4460 5670 4460
Size: 1210 5358
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6568
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5179 136
Size: 107 122 71 631 605 3507 1525
Fragments arranged by size:
3507 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2101 3437 6563 2101
Size: 1336 3126 2106
Fragments arranged by size:
3126 2106 1336
BlpI GC'TnA_GC
Cuts at: 3482 3985 3482
Size: 503 6065
Bme1580I G_kGCm'C
Cuts at: 1790 2119 4629 5785 1790
Size: 329 2510 1156 2573
Fragments arranged by size:
2573 2510 1156 329
BmgBI CAC'GTC
Cuts at: 1318 2207 1318
Size: 889 5679
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2614 4299 5257
Size: 416 53 83 399 24 1197 1685 958
Cuts at: 5257 442
Size: 1753
Fragments arranged by size:
1753 1685 1197 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6568
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5228 1239
Size: 3989 2579
Bpu10I CC'TnA_GC
Cuts at: 2334 3253 2334
Size: 919 5649
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3040 3142 4693 5558 5856 3040
Size: 102 1551 865 298 3752
Fragments arranged by size:
3752 1551 865 298 102
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2778 5231 882
Size: 617 1279 2453 2219
Fragments arranged by size:
2453 2219 1279 617
BsaAI yAC'GTr
Cuts at: 493 1204 2981 4359 493
Size: 711 1777 1378 2702
Fragments arranged by size:
2702 1777 1378 711
BsaBI GATnn'nnATC
Cuts at: 2106 6562 2106
Size: 4456 2112
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2827 4375 4757 5392
Size: 53 83 186 936 1294 1548 382 635
Cuts at: 5392 275
Size: 1451
Fragments arranged by size:
1548 1451 1294 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3723 5001 5399 5673 5820 6231 3723
Size: 1278 398 274 147 411 4060
Fragments arranged by size:
4060 1278 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6343
Fragments arranged by size:
6343 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6568
BseYI C'CCAG_C
Cuts at: 5771 6267 5771
Size: 496 6072
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5995
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3724 4018 4779 5807 1540
Size: 275 186 1723 294 761 1028 2301
Fragments arranged by size:
2301 1723 1028 761 294 275 186
BsiHKAI G_wGCw'C
Cuts at: 2119 4629 4714 5785 2119
Size: 2510 85 1071 2902
Fragments arranged by size:
2902 2510 1071 85
BsmI GAATG_Cn'
Cuts at: 4079 4172 4079
Size: 93 6475
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2119 2261 2436 3887 4629 4714 5785
Size: 399 329 142 175 1451 742 85 1071
Cuts at: 5785 1391
Size: 2174
Fragments arranged by size:
2174 1451 1071 742 399 329 175 142
85
BspEI T'CCGG_A
Cuts at: 6231 6231
Size: 6568
BspHI T'CATG_A
Cuts at: 2082 3123 4457 6187 2082
Size: 1041 1334 1730 2463
Fragments arranged by size:
2463 1730 1334 1041
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2704 2888 2928 3923 844
Size: 1860 184 40 995 3489
Fragments arranged by size:
3489 1860 995 184 40
BsrBI CCG'CTC
Cuts at: 1394 1823 4455 1394
Size: 429 2632 3507
Fragments arranged by size:
3507 2632 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2893 5063 5237 65
Size: 1183 1645 2170 174 1396
Fragments arranged by size:
2170 1645 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5212 5399 1700
Size: 110 3402 187 2869
Fragments arranged by size:
3402 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6568
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6568
BssSI C'ACGA_G
Cuts at: 4628 5640 4628
Size: 1012 5556
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6568
BstBI TT'CG_AA
Cuts at: 5372 5372
Size: 6568
BstEII G'GTnAC_C
Cuts at: 1184 2462 1184
Size: 1278 5290
BstXI CCAn_nnnn'nTGG
Cuts at: 2268 2268
Size: 6568
BstYI r'GATC_y
Cuts at: 1972 2101 3437 3620 3898 4265 4650 4667 6119
Size: 129 1336 183 278 367 385 17 1452
Cuts at: 6119 6205 6217 6563 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1336 385 367 346 278 183
129 86 17 12
BstZ17I GTA'TAC
Cuts at: 3321 3321
Size: 6568
BtgI C'CryG_G
Cuts at: 513 1065 3600 513
Size: 552 2535 3481
Fragments arranged by size:
3481 2535 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5829
Fragments arranged by size:
5829 683 56
BtsI GCAGTG_nn'
Cuts at: 4084 4878 4898 4084
Size: 794 20 5754
Fragments arranged by size:
5754 794 20
ClaI AT'CG_AT
Cuts at: 1554 2292 1554
Size: 738 5830
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6533
DraI TTT'AAA
Cuts at: 3270 3872 3270
Size: 602 5966
DraIII CAC_nnn'GTG
Cuts at: 2622 2622
Size: 6568
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3188 5575 809
Size: 507 1872 2387 1802
Fragments arranged by size:
2387 1872 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2270 3385 4015
Size: 54 1425 50 275 18 440 1115 630
Cuts at: 4015 4904 6533 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1115 889 630 440 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4015 1537
Size: 275 2203 4090
Fragments arranged by size:
4090 2203 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2245 2606 3533 4498 1353
Size: 892 361 927 965 3423
Fragments arranged by size:
3423 965 927 892 361
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5155 5529 5675 387
Size: 1064 138 3566 374 146 1280
Fragments arranged by size:
3566 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2436 3314 4631 5228 6015 6283 1239
Size: 393 804 878 1317 597 787 268 1524
Fragments arranged by size:
1524 1317 878 804 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3877 4255 3877
Size: 378 6190
EcoRV GAT'ATC
Cuts at: 3683 3683
Size: 6568
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2948 2948
Size: 6568
FspI TGC'GCA
Cuts at: 5074 5074
Size: 6568
HaeII r_GCGC'y
Cuts at: 1678 2830 5715 1678
Size: 1152 2885 2531
Fragments arranged by size:
2885 2531 1152
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2524 2556 2775 2807 3656
Size: 32 135 32 952 32 219 32 849
Cuts at: 3656 3688 3824 3856 5209 5241 5283 5315 1373
Size: 32 136 32 1353 32 42 32 2626
Fragments arranged by size:
2626 1353 952 849 219 136 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2683 3912 4158 669
Size: 988 281 745 1229 246 3079
Fragments arranged by size:
3079 1229 988 745 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6568
HpaI GTT'AAC
Cuts at: 4158 4158
Size: 6568
KasI G'GCGC_C
Cuts at: 2826 2826
Size: 6568
KpnI G_GTAC'C
Cuts at: 3206 3893 3206
Size: 687 5881
MfeI C'AATT_G
Cuts at: 2579 4167 2579
Size: 1588 4980
MluI A'CGCG_T
Cuts at: 6444 6444
Size: 6568
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5682 5866 1305
Size: 4377 184 2007
Fragments arranged by size:
4377 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2272 3387 10
Size: 54 1425 343 440 1115 3191
Fragments arranged by size:
3191 1425 1115 440 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2112 2645 3410 3690 3812 4526 4885
Size: 648 946 533 765 280 122 714 359
Cuts at: 4885 5044 518
Size: 159 2042
Fragments arranged by size:
2042 946 765 714 648 533 359 280
159 122
MspA1I CmG'CkG
Cuts at: 1606 2129 3984 4661 5809 6054 1606
Size: 523 1855 677 1148 245 2120
Fragments arranged by size:
2120 1855 1148 677 523 245
NarI GG'CG_CC
Cuts at: 2827 2827
Size: 6568
NcoI C'CATG_G
Cuts at: 513 1065 3600 513
Size: 552 2535 3481
Fragments arranged by size:
3481 2535 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6568
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6568
NotI GC'GGCC_GC
Cuts at: 4015 4015
Size: 6568
NspI r_CATG'y
Cuts at: 1132 1229 3926 5471 1132
Size: 97 2697 1545 2229
Fragments arranged by size:
2697 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6568
PciI A'CATG_T
Cuts at: 5467 5467
Size: 6568
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6568
PmeI GTTT'AAAC
Cuts at: 3872 3872
Size: 6568
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2063 2095 3038 3070 4637 4669 6150 6182 2063
Size: 32 943 32 1567 32 1481 32 2449
Fragments arranged by size:
2449 1567 1481 943 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6568
PsiI TTA'TAA
Cuts at: 4138 4138
Size: 6568
PstI C_TGCA'G
Cuts at: 830 3920 830
Size: 3090 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6568
PvuII CAG'CTG
Cuts at: 2129 2129
Size: 6568
SalI G'TCGA_C
Cuts at: 1936 3910 1936
Size: 1974 4594
SapI GCTCTTCn'nnn_
Cuts at: 3533 3533
Size: 6568
SbfI CC_TGCA'GG
Cuts at: 3920 3920
Size: 6568
ScaI AGT'ACT
Cuts at: 4816 6528 4816
Size: 1712 4856
SfcI C'TryA_G
Cuts at: 826 1045 2543 2665 3916 5051 5732 5923 826
Size: 219 1498 122 1251 1135 681 191 1471
Fragments arranged by size:
1498 1471 1251 1135 681 219 191 122
SfoI GGC'GCC
Cuts at: 2828 2828
Size: 6568
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6568
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6568
SmaI CCC'GGG
Cuts at: 1872 3895 1872
Size: 2023 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3055 3157 4672 5573 5835 820
Size: 197 926 1112 102 1515 901 262 1553
Fragments arranged by size:
1553 1515 1112 926 901 262 197 102
SnaBI TAC'GTA
Cuts at: 493 4359 493
Size: 3866 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6568
SphI G_CATG'C
Cuts at: 1132 3926 1132
Size: 2794 3774
SspI AAT'ATT
Cuts at: 5 52 4492 5
Size: 47 4440 2081
Fragments arranged by size:
4440 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6568
StyI C'CwwG_G
Cuts at: 513 1065 2219 2388 2751 3600 4007 513
Size: 552 1154 169 363 849 407 3074
Fragments arranged by size:
3074 1154 849 552 407 363 169
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2188 2633 4595 4612 4765 4950 5395
Size: 1255 608 445 1962 17 153 185 445
Cuts at: 5395 325
Size: 1498
Fragments arranged by size:
1962 1498 1255 608 445 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4814 6526 96
Size: 274 80 33 51 1443 2837 1712 138
Fragments arranged by size:
2837 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6528 6560 6528
Size: 32 6536
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6568
XbaI T'CTAG_A
Cuts at: 3842 3904 3842
Size: 62 6506
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6568
XmaI C'CCGG_G
Cuts at: 1870 3893 1870
Size: 2023 4545
XmnI GAAnn'nnTTC
Cuts at: 2103 3131 3303 3407 4697 2103
Size: 1028 172 104 1290 3974
Fragments arranged by size:
3974 1290 1028 172 104
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4376 276
Size: 53 83 186 936 2842 2468
Fragments arranged by size:
2842 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI BsiWI BsmBI Bsu36I EcoICRI EcoNI EcoO109I
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SexAI SfiI SrfI SwaI XcmI