Restriction map for
pFN21AE6474
(Circular) (Six-base) MAPSORT of: pFN21AE6474.seq Check: 8492 from: 1 to: 6115
pFN21AE6474 6115 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3925 278
Size: 53 83 186 936 2389 2468
Fragments arranged by size:
2468 2389 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3273 3458 1937
Size: 1336 185 4594
Fragments arranged by size:
4594 1336 185
Acc65I G'GTAC_C
Cuts at: 3436 3436
Size: 6115
AclI AA'CG_TT
Cuts at: 1563 4242 4615 1563
Size: 2679 373 3063
Fragments arranged by size:
3063 2679 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2465 2717 2802 4178 5562 5830 1632
Size: 833 252 85 1376 1384 268 1917
Fragments arranged by size:
1917 1384 1376 833 268 252 85
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5918
AflIII A'CryG_T
Cuts at: 1203 5014 5991 1203
Size: 3811 977 1327
Fragments arranged by size:
3811 1327 977
AgeI A'CCGG_T
Cuts at: 4946 4946
Size: 6115
AhdI GACnn_n'nnGTC
Cuts at: 4844 4844
Size: 6115
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6115
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2146 1184
Size: 962 5153
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2701 2733 2701
Size: 32 6083
AlwNI CAG_nnn'CTG
Cuts at: 2825 3858 5430 2825
Size: 1033 1572 3510
Fragments arranged by size:
3510 1572 1033
ApaLI G'TGCA_C
Cuts at: 4172 5328 4172
Size: 1156 4959
ApoI r'AATT_y
Cuts at: 1493 2094 2442 2808 3424 3650 3802 5911 1493
Size: 601 348 366 616 226 152 2109 1697
Fragments arranged by size:
2109 1697 616 601 366 348 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2076 3125 4669 160
Size: 872 1044 1049 1544 1606
Fragments arranged by size:
1606 1544 1049 1044 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6115
AvaI C'yCGr_G
Cuts at: 1870 1943 3440 1870
Size: 73 1497 4545
Fragments arranged by size:
4545 1497 73
BamHI G'GATC_C
Cuts at: 3044 3445 3812 3044
Size: 401 367 5347
Fragments arranged by size:
5347 401 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3436 4891 618
Size: 325 217 627 1649 1455 1842
Fragments arranged by size:
1842 1649 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2242 3434 1391
Size: 851 1192 4072
Fragments arranged by size:
4072 1192 851
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5144
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4306 4340 1903
Size: 34 2369 34 3678
Fragments arranged by size:
3678 2369 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4007 5217 4007
Size: 1210 4905
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6115
BfrBI ATG'CAT
Cuts at: 2727 2727
Size: 6115
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2180 2964 4726
Size: 107 122 71 631 605 508 784 1762
Cuts at: 4726 136
Size: 1525
Fragments arranged by size:
1762 1525 784 631 605 508 122 107
71
BglII A'GATC_T
Cuts at: 6110 6110
Size: 6115
BlpI GC'TnA_GC
Cuts at: 3532 3532
Size: 6115
Bme1580I G_kGCm'C
Cuts at: 1790 2060 4176 5332 1790
Size: 270 2116 1156 2573
Fragments arranged by size:
2573 2116 1156 270
BmgBI CAC'GTC
Cuts at: 1318 3223 1318
Size: 1905 4210
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3846 4804 442
Size: 416 53 83 399 24 2429 958 1753
Fragments arranged by size:
2429 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2493 1056
Size: 1437 4678
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2556 4775 1239
Size: 1317 2219 2579
Fragments arranged by size:
2579 2219 1317
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2015 2240 4240 5105 5403 2015
Size: 225 2000 865 298 2727
Fragments arranged by size:
2727 2000 865 298 225
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2932 4778 882
Size: 617 1433 1846 2219
Fragments arranged by size:
2219 1846 1433 617
BsaAI yAC'GTr
Cuts at: 493 1204 3277 3906 493
Size: 711 2073 629 2702
Fragments arranged by size:
2702 2073 711 629
BsaBI GATnn'nnATC
Cuts at: 6109 6109
Size: 6115
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3922 4304 4939 275
Size: 53 83 186 936 2389 382 635 1451
Fragments arranged by size:
2389 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4548 4946 5220 5367 5778 4548
Size: 398 274 147 411 4885
Fragments arranged by size:
4885 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2255 2285 1178
Size: 30 165 30 852 30 5008
Fragments arranged by size:
5008 852 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6115
BseYI C'CCAG_C
Cuts at: 2378 5318 5814 2378
Size: 2940 496 2679
Fragments arranged by size:
2940 2679 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2109 1163
Size: 573 373 5169
Fragments arranged by size:
5169 573 373
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3565 4326 5354 1540
Size: 275 186 1564 761 1028 2301
Fragments arranged by size:
2301 1564 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2242 4176 4261 5332 2242
Size: 1934 85 1071 3025
Fragments arranged by size:
3025 1934 1071 85
BsmI GAATG_Cn'
Cuts at: 2593 3626 3719 2593
Size: 1033 93 4989
Fragments arranged by size:
4989 1033 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2060 2242 3434 4176 4261 5332 1391
Size: 399 270 182 1192 742 85 1071 2174
Fragments arranged by size:
2174 1192 1071 742 399 270 182 85
BspEI T'CCGG_A
Cuts at: 5778 5778
Size: 6115
BspHI T'CATG_A
Cuts at: 4004 5734 4004
Size: 1730 4385
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3470 844
Size: 2626 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4002 1394
Size: 429 2179 3507
Fragments arranged by size:
3507 2179 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4610 4784 65
Size: 1183 3362 174 1396
Fragments arranged by size:
3362 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4759 4946 1700
Size: 110 2949 187 2869
Fragments arranged by size:
2949 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6115
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6115
BssSI C'ACGA_G
Cuts at: 2454 4175 5187 2454
Size: 1721 1012 3382
Fragments arranged by size:
3382 1721 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6115
BstBI TT'CG_AA
Cuts at: 4919 4919
Size: 6115
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6115
BstYI r'GATC_y
Cuts at: 1972 3044 3445 3812 4197 4214 5666 5752 5764
Size: 1072 401 367 385 17 1452 86 12
Cuts at: 5764 6110 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1072 401 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2274 513
Size: 552 939 270 4354
Fragments arranged by size:
4354 939 552 270
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5376
Fragments arranged by size:
5376 683 56
BtsI GCAGTG_nn'
Cuts at: 2239 3631 4425 4445 2239
Size: 1392 794 20 3909
Fragments arranged by size:
3909 1392 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6115
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6080
DraI TTT'AAA
Cuts at: 2929 3331 3419 2929
Size: 402 88 5625
Fragments arranged by size:
5625 402 88
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5122 809
Size: 507 3806 1802
Fragments arranged by size:
3806 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2181 2332 2421
Size: 54 1425 50 275 18 351 151 89
Cuts at: 2421 3562 4451 6080 8
Size: 1141 889 1629 43
Fragments arranged by size:
1629 1425 1141 889 351 275 151 89
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3562 1537
Size: 275 1750 4090
Fragments arranged by size:
4090 1750 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2821 3010 4045 1353
Size: 1468 189 1035 3423
Fragments arranged by size:
3423 1468 1035 189
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3160 4702 5076 5222 387
Size: 1064 138 1571 1542 374 146 1280
Fragments arranged by size:
1571 1542 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2240 2240
Size: 6115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2465 2556 2717 2802 4178 4775 5562
Size: 393 833 91 161 85 1376 597 787
Cuts at: 5562 5830 1239
Size: 268 1524
Fragments arranged by size:
1524 1376 833 787 597 393 268 161
91 85
EcoRI G'AATT_C
Cuts at: 2442 3424 3802 2442
Size: 982 378 4755
Fragments arranged by size:
4755 982 378
EcoRV GAT'ATC
Cuts at: 2614 2614
Size: 6115
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2281 3360 2281
Size: 1079 5036
FspI TGC'GCA
Cuts at: 4621 4621
Size: 6115
HaeII r_GCGC'y
Cuts at: 1678 5262 1678
Size: 3584 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2179 2211 4756 4788 4830
Size: 32 135 32 607 32 2545 32 42
Cuts at: 4830 4862 1373
Size: 32 2626
Fragments arranged by size:
2626 2545 607 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2773 3459 3705 669
Size: 988 281 835 686 246 3079
Fragments arranged by size:
3079 988 835 686 281 246
HindIII A'AGCT_T
Cuts at: 748 2221 748
Size: 1473 4642
HpaI GTT'AAC
Cuts at: 2773 3705 2773
Size: 932 5183
KpnI G_GTAC'C
Cuts at: 3440 3440
Size: 6115
MfeI C'AATT_G
Cuts at: 3714 3714
Size: 6115
MluI A'CGCG_T
Cuts at: 5991 5991
Size: 6115
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5229 5413 1305
Size: 3924 184 2007
Fragments arranged by size:
3924 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2183 2334 2423 10
Size: 54 1425 343 351 151 89 3702
Fragments arranged by size:
3702 1425 351 343 151 89 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2138 2866 3221 4073 4432 4591 518
Size: 648 972 728 355 852 359 159 2042
Fragments arranged by size:
2042 972 852 728 648 359 355 159
MspA1I CmG'CkG
Cuts at: 1606 2382 2971 3020 3297 3531 4208 5356 5601
Size: 776 589 49 277 234 677 1148 245
Cuts at: 5601 1606
Size: 2120
Fragments arranged by size:
2120 1148 776 677 589 277 245 234
49
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4624
Fragments arranged by size:
4624 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6115
NheI G'CTAG_C
Cuts at: 1052 2489 1052
Size: 1437 4678
NotI GC'GGCC_GC
Cuts at: 3562 3562
Size: 6115
NsiI A_TGCA'T
Cuts at: 2729 2729
Size: 6115
NspI r_CATG'y
Cuts at: 1132 1229 2673 3473 5018 1132
Size: 97 1444 800 1545 2229
Fragments arranged by size:
2229 1545 1444 800 97
PasI CC'CwG_GG
Cuts at: 1348 2802 1348
Size: 1454 4661
PciI A'CATG_T
Cuts at: 5014 5014
Size: 6115
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2234 2430 1108
Size: 1126 196 4793
Fragments arranged by size:
4793 1126 196
PmeI GTTT'AAAC
Cuts at: 3419 3419
Size: 6115
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4184 4216 5697 5729 4184
Size: 32 1481 32 4570
Fragments arranged by size:
4570 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6115
PsiI TTA'TAA
Cuts at: 3180 3685 3180
Size: 505 5610
PstI C_TGCA'G
Cuts at: 830 2209 3467 830
Size: 1379 1258 3478
Fragments arranged by size:
3478 1379 1258
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6115
PvuII CAG'CTG
Cuts at: 2382 2971 3020 2382
Size: 589 49 5477
Fragments arranged by size:
5477 589 49
SacI G_AGCT'C
Cuts at: 2242 2242
Size: 6115
SalI G'TCGA_C
Cuts at: 1936 3457 1936
Size: 1521 4594
SbfI CC_TGCA'GG
Cuts at: 3467 3467
Size: 6115
ScaI AGT'ACT
Cuts at: 4363 6075 4363
Size: 1712 4403
SfcI C'TryA_G
Cuts at: 826 1045 2205 2754 2826 3463 4598 5279 5470
Size: 219 1160 549 72 637 1135 681 191
Cuts at: 5470 826
Size: 1471
Fragments arranged by size:
1471 1160 1135 681 637 549 219 191
72
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6115
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6115
SmaI CCC'GGG
Cuts at: 1872 3442 1872
Size: 1570 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2030 2255 4219 5120 5382 820
Size: 197 926 87 225 1964 901 262 1553
Fragments arranged by size:
1964 1553 926 901 262 225 197 87
SnaBI TAC'GTA
Cuts at: 493 3906 493
Size: 3413 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6115
SphI G_CATG'C
Cuts at: 1132 2673 3473 1132
Size: 1541 800 3774
Fragments arranged by size:
3774 1541 800
SspI AAT'ATT
Cuts at: 5 52 3388 4039 5
Size: 47 3336 651 2081
Fragments arranged by size:
3336 2081 651 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6115
StyI C'CwwG_G
Cuts at: 513 1065 2004 2641 3554 513
Size: 552 939 637 913 3074
Fragments arranged by size:
3074 939 913 637 552
SwaI ATTT'AAAT
Cuts at: 2929 2929
Size: 6115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2815 4142 4159 4312 4497 4942 325
Size: 1255 1235 1327 17 153 185 445 1498
Fragments arranged by size:
1498 1327 1255 1235 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2355 4361 6073
Size: 274 80 33 51 1443 378 2006 1712
Cuts at: 6073 96
Size: 138
Fragments arranged by size:
2006 1712 1443 378 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2085 2117 6075 6107 2085
Size: 32 3958 32 2093
Fragments arranged by size:
3958 2093 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6115
XbaI T'CTAG_A
Cuts at: 3451 3451
Size: 6115
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2116 2116
Size: 6115
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6115
XmaI C'CCGG_G
Cuts at: 1870 3440 1870
Size: 1570 4545
XmnI GAAnn'nnTTC
Cuts at: 2580 4244 2580
Size: 1664 4451
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3923 276
Size: 53 83 186 936 2389 2468
Fragments arranged by size:
2468 2389 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI