Restriction map for
pFN21AE6472
(Circular) (Six-base) MAPSORT of: pFN21AE6472.seq Check: 3877 from: 1 to: 6091
pFN21AE6472 6091 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2438 2438
Size: 6091
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3901 278
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2415 2874 3434 1937
Size: 478 459 560 4594
Fragments arranged by size:
4594 560 478 459
Acc65I G'GTAC_C
Cuts at: 2633 3412 2633
Size: 779 5312
AclI AA'CG_TT
Cuts at: 1563 4218 4591 1563
Size: 2655 373 3063
Fragments arranged by size:
3063 2655 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2215 2838 4154 5538 5806 1632
Size: 583 623 1316 1384 268 1917
Fragments arranged by size:
1917 1384 1316 623 583 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5894
AflIII A'CryG_T
Cuts at: 1203 2190 4990 5967 1203
Size: 987 2800 977 1327
Fragments arranged by size:
2800 1327 987 977
AgeI A'CCGG_T
Cuts at: 4922 4922
Size: 6091
AhdI GACnn_n'nnGTC
Cuts at: 4820 4820
Size: 6091
AleI CACnn'nnGTG
Cuts at: 1166 3027 1166
Size: 1861 4230
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2282 1184
Size: 1098 4993
AlwNI CAG_nnn'CTG
Cuts at: 3127 3834 5406 3127
Size: 707 1572 3812
Fragments arranged by size:
3812 1572 707
ApaLI G'TGCA_C
Cuts at: 4148 5304 4148
Size: 1156 4935
ApoI r'AATT_y
Cuts at: 1493 3400 3626 3778 5887 1493
Size: 1907 226 152 2109 1697
Fragments arranged by size:
2109 1907 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4645 160
Size: 872 3613 1606
Fragments arranged by size:
3613 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
AvaI C'yCGr_G
Cuts at: 1870 1943 2976 3416 1870
Size: 73 1033 440 4545
Fragments arranged by size:
4545 1033 440 73
BamHI G'GATC_C
Cuts at: 2923 3421 3788 2923
Size: 498 367 5226
Fragments arranged by size:
5226 498 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2150 2426 2633 3073 3412
Size: 325 217 627 363 276 207 440 339
Cuts at: 3412 4867 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 440 363 339 325 276
217 207
BanII G_rGCy'C
Cuts at: 1391 2074 3410 1391
Size: 683 1336 4072
Fragments arranged by size:
4072 1336 683
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2788 2831 3220 928
Size: 971 889 43 389 3799
Fragments arranged by size:
3799 971 889 389 43
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4282 4316 1903
Size: 34 2345 34 3678
Fragments arranged by size:
3678 2345 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3983 5193 3983
Size: 1210 4881
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6091
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4702 136
Size: 107 122 71 631 605 3030 1525
Fragments arranged by size:
3030 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6086 6086
Size: 6091
BlpI GC'TnA_GC
Cuts at: 3508 3508
Size: 6091
Bme1580I G_kGCm'C
Cuts at: 1790 2793 4152 5308 1790
Size: 1003 1359 1156 2573
Fragments arranged by size:
2573 1359 1156 1003
BmgBI CAC'GTC
Cuts at: 1318 2779 2932 3091 1318
Size: 1461 153 159 4318
Fragments arranged by size:
4318 1461 159 153
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3822 4780 442
Size: 416 53 83 399 24 2405 958 1753
Fragments arranged by size:
2405 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6091
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4751 1239
Size: 3512 2579
Bpu10I CC'TnA_GC
Cuts at: 2621 2993 2621
Size: 372 5719
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2068 4216 5081 5379 2068
Size: 2148 865 298 2780
Fragments arranged by size:
2780 2148 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4754 882
Size: 617 3255 2219
Fragments arranged by size:
3255 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3882 493
Size: 711 2678 2702
Fragments arranged by size:
2702 2678 711
BsaBI GATnn'nnATC
Cuts at: 6085 6085
Size: 6091
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3898 4280 4915 275
Size: 53 83 186 936 2365 382 635 1451
Fragments arranged by size:
2365 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4524 4922 5196 5343 5754 4524
Size: 398 274 147 411 4861
Fragments arranged by size:
4861 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2904 2934 1178
Size: 30 165 30 1501 30 4335
Fragments arranged by size:
4335 1501 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3098 1186
Size: 1912 4179
BseYI C'CCAG_C
Cuts at: 2221 5294 5790 2221
Size: 3073 496 2522
Fragments arranged by size:
3073 2522 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2223 3005 1163
Size: 573 487 782 4249
Fragments arranged by size:
4249 782 573 487
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3541 4302 5330 1540
Size: 275 186 1540 761 1028 2301
Fragments arranged by size:
2301 1540 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2054 2937 3003 4152 4237 5308 2054
Size: 883 66 1149 85 1071 2837
Fragments arranged by size:
2837 1149 1071 883 85 66
BsmI GAATG_Cn'
Cuts at: 3602 3695 3602
Size: 93 5998
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2054 2074 2793 2937 3003 3410 4152
Size: 399 264 20 719 144 66 407 742
Cuts at: 4152 4237 5308 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 719 407 399 264 144
85 66 20
BspEI T'CCGG_A
Cuts at: 5754 5754
Size: 6091
BspHI T'CATG_A
Cuts at: 3980 5710 3980
Size: 1730 4361
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2438 3446 844
Size: 1594 1008 3489
Fragments arranged by size:
3489 1594 1008
BsrBI CCG'CTC
Cuts at: 1394 1823 2068 3103 3978 1394
Size: 429 245 1035 875 3507
Fragments arranged by size:
3507 1035 875 429 245
BsrDI GCAATG_nn'
Cuts at: 65 1248 2341 2433 4586 4760 65
Size: 1183 1093 92 2153 174 1396
Fragments arranged by size:
2153 1396 1183 1093 174 92
BsrFI r'CCGG_y
Cuts at: 1700 1810 2716 4735 4922 1700
Size: 110 906 2019 187 2869
Fragments arranged by size:
2869 2019 906 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6091
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6091
BssSI C'ACGA_G
Cuts at: 4151 5163 4151
Size: 1012 5079
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6091
BstBI TT'CG_AA
Cuts at: 4895 4895
Size: 6091
BstEII G'GTnAC_C
Cuts at: 1184 3325 1184
Size: 2141 3950
BstXI CCAn_nnnn'nTGG
Cuts at: 3291 3376 3291
Size: 85 6006
BstYI r'GATC_y
Cuts at: 1972 2923 3379 3421 3788 4173 4190 5642 5728
Size: 951 456 42 367 385 17 1452 86
Cuts at: 5728 5740 6086 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 951 456 385 367 346 86
42 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2853 2964 513
Size: 552 939 849 111 3640
Fragments arranged by size:
3640 939 849 552 111
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2245 2392 487
Size: 683 56 1019 147 4186
Fragments arranged by size:
4186 1019 683 147 56
BtsI GCAGTG_nn'
Cuts at: 2235 2264 2550 2791 3607 4401 4421 2235
Size: 29 286 241 816 794 20 3905
Fragments arranged by size:
3905 816 794 286 241 29 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6091
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6056
DraI TTT'AAA
Cuts at: 3395 3395
Size: 6091
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5098 809
Size: 507 3782 1802
Fragments arranged by size:
3782 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2403 2675 2961
Size: 54 1425 50 275 18 573 272 286
Cuts at: 2961 2967 3207 3349 3538 4427 6056 8
Size: 6 240 142 189 889 1629 43
Fragments arranged by size:
1629 1425 889 573 286 275 272 240
189 142 54 50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 3538 1537
Size: 275 1726 4090
Fragments arranged by size:
4090 1726 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2567 3167 3267 4021 1353
Size: 1214 600 100 754 3423
Fragments arranged by size:
3423 1214 754 600 100
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4678 5052 5198 387
Size: 1064 138 3089 374 146 1280
Fragments arranged by size:
3089 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2215 2838 4154 4751 5538 5806 1239
Size: 393 583 623 1316 597 787 268 1524
Fragments arranged by size:
1524 1316 787 623 597 583 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2343 2343
Size: 6091
EcoRI G'AATT_C
Cuts at: 3400 3778 3400
Size: 378 5713
FspI TGC'GCA
Cuts at: 4597 4597
Size: 6091
HaeII r_GCGC'y
Cuts at: 1678 3161 5238 1678
Size: 1483 2077 2531
Fragments arranged by size:
2531 2077 1483
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2970 3002 3327 3359 4732
Size: 32 135 32 1398 32 325 32 1373
Cuts at: 4732 4764 4806 4838 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1398 1373 325 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2188 3435 3681 669
Size: 988 281 250 1247 246 3079
Fragments arranged by size:
3079 1247 988 281 250 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6091
HpaI GTT'AAC
Cuts at: 3681 3681
Size: 6091
KpnI G_GTAC'C
Cuts at: 2637 3416 2637
Size: 779 5312
MfeI C'AATT_G
Cuts at: 3690 3690
Size: 6091
MluI A'CGCG_T
Cuts at: 2190 5967 2190
Size: 3777 2314
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5205 5389 1305
Size: 3900 184 2007
Fragments arranged by size:
3900 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2405 2677 10
Size: 54 1425 343 573 272 3424
Fragments arranged by size:
3424 1425 573 343 272 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2963 3027 3374 4049 4408 4567 518
Size: 648 1797 64 347 675 359 159 2042
Fragments arranged by size:
2042 1797 675 648 359 347 159 64
MspA1I CmG'CkG
Cuts at: 1606 2511 2849 3291 3507 4184 5332 5577 1606
Size: 905 338 442 216 677 1148 245 2120
Fragments arranged by size:
2120 1148 905 677 442 338 245 216
NaeI GCC'GGC
Cuts at: 2718 2718
Size: 6091
NcoI C'CATG_G
Cuts at: 513 1065 2004 2853 513
Size: 552 939 849 3751
Fragments arranged by size:
3751 939 849 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6091
NgoMIV G'CCGG_C
Cuts at: 2716 2716
Size: 6091
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6091
NotI GC'GGCC_GC
Cuts at: 3538 3538
Size: 6091
NspI r_CATG'y
Cuts at: 1132 1229 2809 3449 4994 1132
Size: 97 1580 640 1545 2229
Fragments arranged by size:
2229 1580 1545 640 97
PasI CC'CwG_GG
Cuts at: 1348 2586 2696 3233 3331 1348
Size: 1238 110 537 98 4108
Fragments arranged by size:
4108 1238 537 110 98
PciI A'CATG_T
Cuts at: 4990 4990
Size: 6091
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2356 3294 1108
Size: 1248 938 3905
Fragments arranged by size:
3905 1248 938
PfoI T'CCnGG_A
Cuts at: 2723 2723
Size: 6091
PmeI GTTT'AAAC
Cuts at: 3395 3395
Size: 6091
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4160 4192 5673 5705 4160
Size: 32 1481 32 4546
Fragments arranged by size:
4546 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2343 2343
Size: 6091
PshAI GACnn'nnGTC
Cuts at: 1613 3128 1613
Size: 1515 4576
PsiI TTA'TAA
Cuts at: 3661 3661
Size: 6091
PstI C_TGCA'G
Cuts at: 830 2085 2271 3123 3443 830
Size: 1255 186 852 320 3478
Fragments arranged by size:
3478 1255 852 320 186
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6091
PvuII CAG'CTG
Cuts at: 2849 3291 2849
Size: 442 5649
SalI G'TCGA_C
Cuts at: 1936 3433 1936
Size: 1497 4594
SapI GCTCTTCn'nnn_
Cuts at: 3167 3167
Size: 6091
SbfI CC_TGCA'GG
Cuts at: 3443 3443
Size: 6091
ScaI AGT'ACT
Cuts at: 4339 6051 4339
Size: 1712 4379
SexAI A'CCwGG_T
Cuts at: 2636 2636
Size: 6091
SfcI C'TryA_G
Cuts at: 826 1045 2032 2081 2267 3119 3439 4574 5255
Size: 219 987 49 186 852 320 1135 681
Cuts at: 5255 5446 826
Size: 191 1471
Fragments arranged by size:
1471 1135 987 852 681 320 219 191
186 49
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6091
SmaI CCC'GGG
Cuts at: 1872 3418 1872
Size: 1546 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2047 4195 5096 5358 820
Size: 197 926 104 2148 901 262 1553
Fragments arranged by size:
2148 1553 926 901 262 197 104
SnaBI TAC'GTA
Cuts at: 493 3882 493
Size: 3389 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6091
SphI G_CATG'C
Cuts at: 1132 2809 3449 1132
Size: 1677 640 3774
Fragments arranged by size:
3774 1677 640
SspI AAT'ATT
Cuts at: 5 52 4015 5
Size: 47 3963 2081
Fragments arranged by size:
3963 2081 47
StuI AGG'CCT
Cuts at: 1517 3051 3204 1517
Size: 1534 153 4404
Fragments arranged by size:
4404 1534 153
StyI C'CwwG_G
Cuts at: 513 1065 2004 2516 2853 3530 513
Size: 552 939 512 337 677 3074
Fragments arranged by size:
3074 939 677 552 512 337
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2189 3286 4118 4135 4288 4473 4918
Size: 1255 609 1097 832 17 153 185 445
Cuts at: 4918 325
Size: 1498
Fragments arranged by size:
1498 1255 1097 832 609 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2667 4337 6049
Size: 274 80 33 51 1443 690 1670 1712
Cuts at: 6049 96
Size: 138
Fragments arranged by size:
1712 1670 1443 690 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2904 2936 3038 3070 6051 6083 2904
Size: 32 102 32 2981 32 2912
Fragments arranged by size:
2981 2912 102 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6091
XbaI T'CTAG_A
Cuts at: 3427 3427
Size: 6091
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3291 3291
Size: 6091
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6091
XmaI C'CCGG_G
Cuts at: 1870 3416 1870
Size: 1546 4545
XmnI GAAnn'nnTTC
Cuts at: 4220 4220
Size: 6091
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3899 276
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SfiI SfoI SrfI SwaI