Restriction map for
pFN21AE6470
(Circular) (Six-base) MAPSORT of: pFN21AE6470.seq Check: 5571 from: 1 to: 6217
pFN21AE6470 6217 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4027 278
Size: 53 83 186 936 2491 2468
Fragments arranged by size:
2491 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3560 1937
Size: 1623 4594
Acc65I G'GTAC_C
Cuts at: 3538 3538
Size: 6217
AclI AA'CG_TT
Cuts at: 1563 2724 4344 4717 1563
Size: 1161 1620 373 3063
Fragments arranged by size:
3063 1620 1161 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2420 4280 5664 5932 1632
Size: 788 1860 1384 268 1917
Fragments arranged by size:
1917 1860 1384 788 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6020
AflIII A'CryG_T
Cuts at: 1203 5116 6093 1203
Size: 3913 977 1327
Fragments arranged by size:
3913 1327 977
AgeI A'CCGG_T
Cuts at: 5048 5048
Size: 6217
AhdI GACnn_n'nnGTC
Cuts at: 4946 4946
Size: 6217
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6217
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6217
AlwNI CAG_nnn'CTG
Cuts at: 3960 5532 3960
Size: 1572 4645
ApaLI G'TGCA_C
Cuts at: 4274 5430 4274
Size: 1156 5061
ApoI r'AATT_y
Cuts at: 1493 2095 3060 3146 3176 3312 3481 3487 3526
Size: 602 965 86 30 136 169 6 39
Cuts at: 3526 3752 3904 6013 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 965 602 226 169 152 136
86 39 30 6
AseI AT'TA_AT
Cuts at: 160 1032 4771 160
Size: 872 3739 1606
Fragments arranged by size:
3739 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
AvaI C'yCGr_G
Cuts at: 1870 1943 3542 1870
Size: 73 1599 4545
Fragments arranged by size:
4545 1599 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2829 2862 2829
Size: 33 6184
BamHI G'GATC_C
Cuts at: 3547 3914 3547
Size: 367 5850
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3538 4993 618
Size: 325 217 627 1751 1455 1842
Fragments arranged by size:
1842 1751 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3536 1391
Size: 2145 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2426 2512 2668 928
Size: 971 527 86 156 4477
Fragments arranged by size:
4477 971 527 156 86
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4408 4442 1903
Size: 34 2471 34 3678
Fragments arranged by size:
3678 2471 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4109 5319 4109
Size: 1210 5007
BclI T'GATC_A
Cuts at: 1548 2167 1548
Size: 619 5598
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2039 4828 136
Size: 107 122 71 631 605 367 2789 1525
Fragments arranged by size:
2789 1525 631 605 367 122 107 71
BglII A'GATC_T
Cuts at: 6212 6212
Size: 6217
BlpI GC'TnA_GC
Cuts at: 3634 3634
Size: 6217
Bme1580I G_kGCm'C
Cuts at: 1790 4278 5434 1790
Size: 2488 1156 2573
Fragments arranged by size:
2573 2488 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6217
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3948 4906 442
Size: 416 53 83 399 24 2531 958 1753
Fragments arranged by size:
2531 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6217
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2582 3402 4877 1239
Size: 1343 820 1475 2579
Fragments arranged by size:
2579 1475 1343 820
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2901 4342 5207 5505 2901
Size: 1441 865 298 3613
Fragments arranged by size:
3613 1441 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4880 882
Size: 617 3381 2219
Fragments arranged by size:
3381 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4008 493
Size: 711 2804 2702
Fragments arranged by size:
2804 2702 711
BsaBI GATnn'nnATC
Cuts at: 3125 6211 3125
Size: 3086 3131
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2021 4024 4406 5041
Size: 53 83 186 936 488 2003 382 635
Cuts at: 5041 275
Size: 1451
Fragments arranged by size:
2003 1451 936 635 488 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4650 5048 5322 5469 5880 4650
Size: 398 274 147 411 4987
Fragments arranged by size:
4987 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5992
Fragments arranged by size:
5992 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2066 1186
Size: 880 5337
BseYI C'CCAG_C
Cuts at: 2494 5420 5916 2494
Size: 2926 496 2795
Fragments arranged by size:
2926 2795 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5644
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3667 4428 5456 1540
Size: 275 186 1666 761 1028 2301
Fragments arranged by size:
2301 1666 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4278 4363 5434 4278
Size: 85 1071 5061
Fragments arranged by size:
5061 1071 85
BsmI GAATG_Cn'
Cuts at: 3374 3728 3821 3374
Size: 354 93 5770
Fragments arranged by size:
5770 354 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3536 4278 4363 5434 1391
Size: 399 1746 742 85 1071 2174
Fragments arranged by size:
2174 1746 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5880 5880
Size: 6217
BspHI T'CATG_A
Cuts at: 2548 4106 5836 2548
Size: 1558 1730 2929
Fragments arranged by size:
2929 1730 1558
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3572 844
Size: 2728 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4104 1394
Size: 429 2281 3507
Fragments arranged by size:
3507 2281 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4712 4886 65
Size: 1183 3464 174 1396
Fragments arranged by size:
3464 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2023 4861 5048 1700
Size: 110 213 2838 187 2869
Fragments arranged by size:
2869 2838 213 187 110
BsrGI T'GTAC_A
Cuts at: 96 2188 96
Size: 2092 4125
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6217
BssSI C'ACGA_G
Cuts at: 4277 5289 4277
Size: 1012 5205
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6217
BstBI TT'CG_AA
Cuts at: 3326 5021 3326
Size: 1695 4522
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6217
BstYI r'GATC_y
Cuts at: 1972 2782 3188 3547 3914 4299 4316 5768 5854
Size: 810 406 359 367 385 17 1452 86
Cuts at: 5854 5866 6212 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 810 406 385 367 359 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2730 3130 513
Size: 552 939 726 400 3600
Fragments arranged by size:
3600 939 726 552 400
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5478
Fragments arranged by size:
5478 683 56
BtsI GCAGTG_nn'
Cuts at: 3733 4527 4547 3733
Size: 794 20 5403
Fragments arranged by size:
5403 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6217
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6182
DraI TTT'AAA
Cuts at: 2368 3021 3521 2368
Size: 653 500 5064
Fragments arranged by size:
5064 653 500
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5224 809
Size: 507 3908 1802
Fragments arranged by size:
3908 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3285 3664 4553
Size: 54 1425 50 275 18 1455 379 889
Cuts at: 4553 6182 8
Size: 1629 43
Fragments arranged by size:
1629 1455 1425 889 379 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3664 1537
Size: 275 1852 4090
Fragments arranged by size:
4090 1852 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4147 1353
Size: 2794 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2473 4804 5178 5324 387
Size: 1064 138 884 2331 374 146 1280
Fragments arranged by size:
2331 1280 1064 884 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2420 2582 3402 4280 4877 5664 5932
Size: 393 788 162 820 878 597 787 268
Cuts at: 5932 1239
Size: 1524
Fragments arranged by size:
1524 878 820 788 787 597 393 268
162
EcoO109I rG'GnC_Cy
Cuts at: 2076 2076
Size: 6217
EcoRI G'AATT_C
Cuts at: 3487 3526 3904 3487
Size: 39 378 5800
Fragments arranged by size:
5800 378 39
FspI TGC'GCA
Cuts at: 4723 4723
Size: 6217
HaeII r_GCGC'y
Cuts at: 1678 5364 1678
Size: 3686 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2093 2125 4858 4890 4932
Size: 32 135 32 521 32 2733 32 42
Cuts at: 4932 4964 1373
Size: 32 2626
Fragments arranged by size:
2733 2626 521 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2376 2470 2626 3561 3807 669
Size: 988 281 438 94 156 935 246 3079
Fragments arranged by size:
3079 988 935 438 281 246 156 94
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6217
HpaI GTT'AAC
Cuts at: 3807 3807
Size: 6217
KpnI G_GTAC'C
Cuts at: 3542 3542
Size: 6217
MfeI C'AATT_G
Cuts at: 3816 3816
Size: 6217
MluI A'CGCG_T
Cuts at: 6093 6093
Size: 6217
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2792 3241 5331 5515 1305
Size: 1487 449 2090 184 2007
Fragments arranged by size:
2090 2007 1487 449 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3287 10
Size: 54 1425 343 1455 2940
Fragments arranged by size:
2940 1455 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2867 3083 4175 4534 4693 518
Size: 648 1701 216 1092 359 159 2042
Fragments arranged by size:
2042 1701 1092 648 359 216 159
MspA1I CmG'CkG
Cuts at: 1606 2494 3633 4310 5458 5703 1606
Size: 888 1139 677 1148 245 2120
Fragments arranged by size:
2120 1148 1139 888 677 245
NaeI GCC'GGC
Cuts at: 2025 2025
Size: 6217
NcoI C'CATG_G
Cuts at: 513 1065 2004 2730 3130 513
Size: 552 939 726 400 3600
Fragments arranged by size:
3600 939 726 552 400
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6217
NgoMIV G'CCGG_C
Cuts at: 2023 2023
Size: 6217
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6217
NotI GC'GGCC_GC
Cuts at: 3664 3664
Size: 6217
NspI r_CATG'y
Cuts at: 1132 1229 2779 3498 3575 5120 1132
Size: 97 1550 719 77 1545 2229
Fragments arranged by size:
2229 1550 1545 719 97 77
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6217
PciI A'CATG_T
Cuts at: 5116 5116
Size: 6217
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6217
PfoI T'CCnGG_A
Cuts at: 2559 2559
Size: 6217
PmeI GTTT'AAAC
Cuts at: 3521 3521
Size: 6217
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4286 4318 5799 5831 4286
Size: 32 1481 32 4672
Fragments arranged by size:
4672 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6217
PsiI TTA'TAA
Cuts at: 2663 3339 3787 2663
Size: 676 448 5093
Fragments arranged by size:
5093 676 448
PstI C_TGCA'G
Cuts at: 830 3569 830
Size: 2739 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6217
PvuII CAG'CTG
Cuts at: 2494 2494
Size: 6217
SalI G'TCGA_C
Cuts at: 1936 3559 1936
Size: 1623 4594
SbfI CC_TGCA'GG
Cuts at: 3569 3569
Size: 6217
ScaI AGT'ACT
Cuts at: 4465 6177 4465
Size: 1712 4505
SfcI C'TryA_G
Cuts at: 826 1045 2503 3369 3565 4700 5381 5572 826
Size: 219 1458 866 196 1135 681 191 1471
Fragments arranged by size:
1471 1458 1135 866 681 219 196 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
SgrAI Cr'CCGG_yG
Cuts at: 1700 2023 1700
Size: 323 5894
SmaI CCC'GGG
Cuts at: 1872 3544 1872
Size: 1672 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2916 4321 5222 5484 820
Size: 197 926 973 1405 901 262 1553
Fragments arranged by size:
1553 1405 973 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4008 493
Size: 3515 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6217
SphI G_CATG'C
Cuts at: 1132 3575 1132
Size: 2443 3774
SspI AAT'ATT
Cuts at: 5 52 2221 2929 3364 4141 5
Size: 47 2169 708 435 777 2081
Fragments arranged by size:
2169 2081 777 708 435 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6217
StyI C'CwwG_G
Cuts at: 513 1065 2004 2423 2730 3130 3656 513
Size: 552 939 419 307 400 526 3074
Fragments arranged by size:
3074 939 552 526 419 400 307
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4244 4261 4414 4599 5044 325
Size: 1255 2664 17 153 185 445 1498
Fragments arranged by size:
2664 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2188 2704 4463
Size: 274 80 33 51 1443 211 516 1759
Cuts at: 4463 6175 96
Size: 1712 138
Fragments arranged by size:
1759 1712 1443 516 274 211 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6177 6209 6177
Size: 32 6185
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6217
XbaI T'CTAG_A
Cuts at: 3553 3553
Size: 6217
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2728 2728
Size: 6217
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6217
XmaI C'CCGG_G
Cuts at: 1870 3542 1870
Size: 1672 4545
XmnI GAAnn'nnTTC
Cuts at: 2308 4346 2308
Size: 2038 4179
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4025 276
Size: 53 83 186 936 2491 2468
Fragments arranged by size:
2491 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI