Restriction map for
pFN21AE6469
(Circular) (Six-base) MAPSORT of: pFN21AE6469.seq Check: 8931 from: 1 to: 6295
pFN21AE6469 6295 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2147 2147
Size: 6295
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2265 4105 278
Size: 53 83 186 936 729 1840 2468
Fragments arranged by size:
2468 1840 936 729 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3062 3638 1937
Size: 1125 576 4594
Fragments arranged by size:
4594 1125 576
Acc65I G'GTAC_C
Cuts at: 3616 3616
Size: 6295
AclI AA'CG_TT
Cuts at: 1563 2706 4422 4795 1563
Size: 1143 1716 373 3063
Fragments arranged by size:
3063 1716 1143 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2385 2855 3282 4358 5742 6010 1632
Size: 753 470 427 1076 1384 268 1917
Fragments arranged by size:
1917 1384 1076 753 470 427 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6098
AflIII A'CryG_T
Cuts at: 1203 5194 6171 1203
Size: 3991 977 1327
Fragments arranged by size:
3991 1327 977
AgeI A'CCGG_T
Cuts at: 5126 5126
Size: 6295
AhdI GACnn_n'nnGTC
Cuts at: 5024 5024
Size: 6295
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6295
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2207 1184
Size: 1023 5272
AlwNI CAG_nnn'CTG
Cuts at: 4038 5610 4038
Size: 1572 4723
ApaI G_GGCC'C
Cuts at: 2053 2053
Size: 6295
ApaLI G'TGCA_C
Cuts at: 4352 5508 4352
Size: 1156 5139
ApoI r'AATT_y
Cuts at: 1493 2208 2798 3004 3604 3830 3982 6091 1493
Size: 715 590 206 600 226 152 2109 1697
Fragments arranged by size:
2109 1697 715 600 590 226 206 152
AseI AT'TA_AT
Cuts at: 160 1032 2880 4849 160
Size: 872 1848 1969 1606
Fragments arranged by size:
1969 1848 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6295
AvaI C'yCGr_G
Cuts at: 1870 1943 2085 3620 1870
Size: 73 142 1535 4545
Fragments arranged by size:
4545 1535 142 73
BamHI G'GATC_C
Cuts at: 3625 3992 3625
Size: 367 5928
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2129 2648 3616 5071 618
Size: 325 217 627 342 519 968 1455 1842
Fragments arranged by size:
1842 1455 968 627 519 342 325 217
BanII G_rGCy'C
Cuts at: 1391 2053 3304 3614 1391
Size: 662 1251 310 4072
Fragments arranged by size:
4072 1251 662 310
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2551 3576 928
Size: 971 652 1025 3647
Fragments arranged by size:
3647 1025 971 652
BbvCI CC'TCA_GC
Cuts at: 2037 2037
Size: 6295
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4486 4520 1903
Size: 34 2549 34 3678
Fragments arranged by size:
3678 2549 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4187 5397 4187
Size: 1210 5085
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6295
BfrBI ATG'CAT
Cuts at: 2687 2687
Size: 6295
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4906 136
Size: 107 122 71 631 605 3234 1525
Fragments arranged by size:
3234 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3016 6290 3016
Size: 3274 3021
BlpI GC'TnA_GC
Cuts at: 3408 3712 3408
Size: 304 5991
Bme1580I G_kGCm'C
Cuts at: 1790 2053 2653 4356 5512 1790
Size: 263 600 1703 1156 2573
Fragments arranged by size:
2573 1703 1156 600 263
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6295
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4026 4984 442
Size: 416 53 83 399 24 2609 958 1753
Fragments arranged by size:
2609 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6295
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2755 2755
Size: 6295
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3252 4955 1239
Size: 2013 1703 2579
Fragments arranged by size:
2579 2013 1703
Bpu10I CC'TnA_GC
Cuts at: 2037 2253 2037
Size: 216 6079
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4420 5285 5583 4420
Size: 865 298 5132
Fragments arranged by size:
5132 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4958 882
Size: 617 3459 2219
Fragments arranged by size:
3459 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4086 493
Size: 711 2882 2702
Fragments arranged by size:
2882 2702 711
BsaBI GATnn'nnATC
Cuts at: 6289 6289
Size: 6295
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2262 4102 4484 5119
Size: 53 83 186 936 729 1840 382 635
Cuts at: 5119 275
Size: 1451
Fragments arranged by size:
1840 1451 936 729 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4728 5126 5400 5547 5958 4728
Size: 398 274 147 411 5065
Fragments arranged by size:
5065 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6070
Fragments arranged by size:
6070 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3110 1186
Size: 1924 4371
BseYI C'CCAG_C
Cuts at: 3575 5498 5994 3575
Size: 1923 496 3876
Fragments arranged by size:
3876 1923 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2175 2330 1163
Size: 573 439 155 5128
Fragments arranged by size:
5128 573 439 155
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3745 4506 5534 1540
Size: 275 186 1744 761 1028 2301
Fragments arranged by size:
2301 1744 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2854 3304 4356 4441 5512 2854
Size: 450 1052 85 1071 3637
Fragments arranged by size:
3637 1071 1052 450 85
BsmI GAATG_Cn'
Cuts at: 3370 3806 3899 3370
Size: 436 93 5766
Fragments arranged by size:
5766 436 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2053 2653 2854 3304 3614 4356 4441
Size: 399 263 600 201 450 310 742 85
Cuts at: 4441 5512 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 600 450 399 310 263
201 85
BspEI T'CCGG_A
Cuts at: 5958 5958
Size: 6295
BspHI T'CATG_A
Cuts at: 4184 5914 4184
Size: 1730 4565
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2147 3650 844
Size: 1303 1503 3489
Fragments arranged by size:
3489 1503 1303
BsrBI CCG'CTC
Cuts at: 1394 1823 4182 1394
Size: 429 2359 3507
Fragments arranged by size:
3507 2359 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2191 3261 4790 4964 65
Size: 1183 943 1070 1529 174 1396
Fragments arranged by size:
1529 1396 1183 1070 943 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4939 5126 1700
Size: 110 3129 187 2869
Fragments arranged by size:
3129 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2328 96
Size: 2232 4063
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6295
BssSI C'ACGA_G
Cuts at: 4355 5367 4355
Size: 1012 5283
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2101 3476 794
Size: 1307 1375 3613
Fragments arranged by size:
3613 1375 1307
BstBI TT'CG_AA
Cuts at: 5099 5099
Size: 6295
BstEII G'GTnAC_C
Cuts at: 1184 2552 1184
Size: 1368 4927
BstYI r'GATC_y
Cuts at: 1972 3016 3625 3992 4377 4394 5846 5932 5944
Size: 1044 609 367 385 17 1452 86 12
Cuts at: 5944 6290 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1044 609 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 3063 3063
Size: 6295
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4804
Fragments arranged by size:
4804 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5556
Fragments arranged by size:
5556 683 56
BtsI GCAGTG_nn'
Cuts at: 3811 4605 4625 3811
Size: 794 20 5481
Fragments arranged by size:
5481 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6295
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3231 3266 529
Size: 35 2667 35 3558
Fragments arranged by size:
3558 2667 35 35
DraI TTT'AAA
Cuts at: 3599 3599
Size: 6295
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3045 5302 809
Size: 507 1729 2257 1802
Fragments arranged by size:
2257 1802 1729 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2997 3742 4631
Size: 54 1425 50 275 18 1167 745 889
Cuts at: 4631 6260 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1167 889 745 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3742 1537
Size: 275 1930 4090
Fragments arranged by size:
4090 1930 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2573 2760 2946 3292 4225 1353
Size: 1220 187 186 346 933 3423
Fragments arranged by size:
3423 1220 933 346 187 186
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4882 5256 5402 387
Size: 1064 138 3293 374 146 1280
Fragments arranged by size:
3293 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3302 3302
Size: 6295
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2385 2855 3252 3282 4358 4955 5742
Size: 393 753 470 397 30 1076 597 787
Cuts at: 5742 6010 1239
Size: 268 1524
Fragments arranged by size:
1524 1076 787 753 597 470 397 393
268 30
EcoO109I rG'GnC_Cy
Cuts at: 2120 2341 2120
Size: 221 6074
EcoRI G'AATT_C
Cuts at: 3004 3604 3982 3004
Size: 600 378 5317
Fragments arranged by size:
5317 600 378
FspI TGC'GCA
Cuts at: 4801 4801
Size: 6295
HaeII r_GCGC'y
Cuts at: 1678 5442 1678
Size: 3764 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2859 2891 3571 3603 4936
Size: 32 135 32 1287 32 680 32 1333
Cuts at: 4936 4968 5010 5042 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1333 1287 680 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2026 3106 3639 3885 669
Size: 988 281 88 1080 533 246 3079
Fragments arranged by size:
3079 1080 988 533 281 246 88
HindIII A'AGCT_T
Cuts at: 748 3318 748
Size: 2570 3725
HpaI GTT'AAC
Cuts at: 3106 3885 3106
Size: 779 5516
KpnI G_GTAC'C
Cuts at: 3620 3620
Size: 6295
MfeI C'AATT_G
Cuts at: 3243 3894 3243
Size: 651 5644
MluI A'CGCG_T
Cuts at: 6171 6171
Size: 6295
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2243 5409 5593 1305
Size: 938 3166 184 2007
Fragments arranged by size:
3166 2007 938 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4473
Fragments arranged by size:
4473 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2138 4253 4612 4771 518
Size: 648 972 2115 359 159 2042
Fragments arranged by size:
2115 2042 972 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3711 4388 5536 5781 1606
Size: 2105 677 1148 245 2120
Fragments arranged by size:
2120 2105 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4804
Fragments arranged by size:
4804 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6295
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6295
NotI GC'GGCC_GC
Cuts at: 3742 3742
Size: 6295
NsiI A_TGCA'T
Cuts at: 2689 2689
Size: 6295
NspI r_CATG'y
Cuts at: 1132 1229 3044 3416 3653 5198 1132
Size: 97 1815 372 237 1545 2229
Fragments arranged by size:
2229 1815 1545 372 237 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6295
PciI A'CATG_T
Cuts at: 5194 5194
Size: 6295
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6295
PfoI T'CCnGG_A
Cuts at: 3229 3229
Size: 6295
PmeI GTTT'AAAC
Cuts at: 3599 3599
Size: 6295
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4364 4396 5877 5909 4364
Size: 32 1481 32 4750
Fragments arranged by size:
4750 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2120 2120
Size: 6295
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6295
PsiI TTA'TAA
Cuts at: 2754 3865 2754
Size: 1111 5184
PspOMI G'GGCC_C
Cuts at: 2049 2049
Size: 6295
PstI C_TGCA'G
Cuts at: 830 2097 2764 3647 830
Size: 1267 667 883 3478
Fragments arranged by size:
3478 1267 883 667
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6295
SacI G_AGCT'C
Cuts at: 3304 3304
Size: 6295
SalI G'TCGA_C
Cuts at: 1936 3637 1936
Size: 1701 4594
SapI GCTCTTCn'nnn_
Cuts at: 2946 3292 2946
Size: 346 5949
SbfI CC_TGCA'GG
Cuts at: 3647 3647
Size: 6295
ScaI AGT'ACT
Cuts at: 4543 6255 4543
Size: 1712 4583
SexAI A'CCwGG_T
Cuts at: 2548 2548
Size: 6295
SfcI C'TryA_G
Cuts at: 826 1045 2093 2760 3024 3643 4778 5459 5650
Size: 219 1048 667 264 619 1135 681 191
Cuts at: 5650 826
Size: 1471
Fragments arranged by size:
1471 1135 1048 681 667 619 264 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6295
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6295
SmaI CCC'GGG
Cuts at: 1872 3622 1872
Size: 1750 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4399 5300 5562 820
Size: 197 926 2456 901 262 1553
Fragments arranged by size:
2456 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4086 493
Size: 3593 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6295
SphI G_CATG'C
Cuts at: 1132 3653 1132
Size: 2521 3774
SspI AAT'ATT
Cuts at: 5 52 4219 5
Size: 47 4167 2081
Fragments arranged by size:
4167 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6295
StyI C'CwwG_G
Cuts at: 513 1065 2004 2031 2626 3348 3734 513
Size: 552 939 27 595 722 386 3074
Fragments arranged by size:
3074 939 722 595 552 386 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4322 4339 4492 4677 5122 325
Size: 1255 2742 17 153 185 445 1498
Fragments arranged by size:
2742 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2188 2328 2815
Size: 274 80 33 51 1443 211 140 487
Cuts at: 2815 4541 6253 96
Size: 1726 1712 138
Fragments arranged by size:
1726 1712 1443 487 274 211 140 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2985 3017 6255 6287 2985
Size: 32 3238 32 2993
Fragments arranged by size:
3238 2993 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2843 1316
Size: 1527 4768
XbaI T'CTAG_A
Cuts at: 3631 3631
Size: 6295
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2038 3550 2038
Size: 1512 4783
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6295
XmaI C'CCGG_G
Cuts at: 1870 3620 1870
Size: 1750 4545
XmnI GAAnn'nnTTC
Cuts at: 4424 4424
Size: 6295
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2263 4103 276
Size: 53 83 186 936 729 1840 2468
Fragments arranged by size:
2468 1840 936 729 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SacI SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BsiWI BsmBI
BstXI Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI
PsrI PvuII RsrII SacII SanDI SfiI SfoI SrfI
SwaI