(Circular) (Six-base) MAPSORT of: pFN21AE6468.seq Check: 578 from: 1 to: 6481 pFN21AE6468 6481 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2998 3294 2998 Size: 296 6185 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4291 278 Size: 53 83 186 936 2755 2468 Fragments arranged by size: 2755 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3824 1937 Size: 1887 4594 Acc65I G'GTAC_C Cuts at: 2337 3802 2337 Size: 1465 5016 AclI AA'CG_TT Cuts at: 1563 4608 4981 1563 Size: 3045 373 3063 Fragments arranged by size: 3063 3045 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3626 4544 5928 6196 1632 Size: 1994 918 1384 268 1917 Fragments arranged by size: 1994 1917 1384 918 268 AflII C'TTAA_G Cuts at: 820 1017 3482 820 Size: 197 2465 3819 Fragments arranged by size: 3819 2465 197 AflIII A'CryG_T Cuts at: 1203 2085 2682 3450 5380 6357 1203 Size: 882 597 768 1930 977 1327 Fragments arranged by size: 1930 1327 977 882 768 597 AgeI A'CCGG_T Cuts at: 5312 5312 Size: 6481 AhdI GACnn_n'nnGTC Cuts at: 5210 5210 Size: 6481 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6481 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6481 AlwNI CAG_nnn'CTG Cuts at: 4224 5796 4224 Size: 1572 4909 ApaLI G'TGCA_C Cuts at: 4538 5694 4538 Size: 1156 5325 ApoI r'AATT_y Cuts at: 1493 3790 4016 4168 6277 1493 Size: 2297 226 152 2109 1697 Fragments arranged by size: 2297 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5035 160 Size: 872 4003 1606 Fragments arranged by size: 4003 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6481 AvaI C'yCGr_G Cuts at: 1870 1943 3806 1870 Size: 73 1863 4545 Fragments arranged by size: 4545 1863 73 BamHI G'GATC_C Cuts at: 2359 3811 4178 2359 Size: 1452 367 4662 Fragments arranged by size: 4662 1452 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2228 2337 2567 3802 5257 Size: 325 217 627 441 109 230 1235 1455 Cuts at: 5257 618 Size: 1842 Fragments arranged by size: 1842 1455 1235 627 441 325 230 217 109 BanII G_rGCy'C Cuts at: 1391 3800 1391 Size: 2409 4072 BbeI G_GCGC'C Cuts at: 2232 2232 Size: 6481 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3562 928 Size: 971 1663 3847 Fragments arranged by size: 3847 1663 971 BbvCI CC'TCA_GC Cuts at: 3461 3461 Size: 6481 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2764 2798 4672 4706 1903 Size: 34 827 34 1874 34 3678 Fragments arranged by size: 3678 1874 827 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4373 5583 4373 Size: 1210 5271 BclI T'GATC_A Cuts at: 1548 1548 Size: 6481 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5092 136 Size: 107 122 71 631 605 3420 1525 Fragments arranged by size: 3420 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2745 6476 2745 Size: 3731 2750 BlpI GC'TnA_GC Cuts at: 3898 3898 Size: 6481 Bme1580I G_kGCm'C Cuts at: 1790 3116 4542 5698 1790 Size: 1326 1426 1156 2573 Fragments arranged by size: 2573 1426 1326 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6481 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4212 5170 442 Size: 416 53 83 399 24 2795 958 1753 Fragments arranged by size: 2795 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6481 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3319 3319 Size: 6481 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2254 2468 3023 3262 5141 1239 Size: 1015 214 555 239 1879 2579 Fragments arranged by size: 2579 1879 1015 555 239 214 Bpu10I CC'TnA_GC Cuts at: 3461 3578 3461 Size: 117 6364 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3737 4606 5471 5769 3737 Size: 869 865 298 4449 Fragments arranged by size: 4449 869 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2290 5144 882 Size: 617 791 2854 2219 Fragments arranged by size: 2854 2219 791 617 BsaAI yAC'GTr Cuts at: 493 1204 3451 4272 493 Size: 711 2247 821 2702 Fragments arranged by size: 2702 2247 821 711 BsaBI GATnn'nnATC Cuts at: 6475 6475 Size: 6481 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2229 2429 2603 3624 Size: 53 83 186 936 696 200 174 1021 Cuts at: 3624 4288 4670 5305 275 Size: 664 382 635 1451 Fragments arranged by size: 1451 1021 936 696 664 635 382 200 186 174 83 53 BsaWI w'CCGG_w Cuts at: 4914 5312 5586 5733 6144 4914 Size: 398 274 147 411 5251 Fragments arranged by size: 5251 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6256 Fragments arranged by size: 6256 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2429 2516 3478 3688 1186 Size: 1243 87 962 210 3979 Fragments arranged by size: 3979 1243 962 210 87 BseYI C'CCAG_C Cuts at: 3168 3208 3226 3359 5684 6180 3168 Size: 40 18 133 2325 496 3469 Fragments arranged by size: 3469 2325 496 133 40 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2522 1163 Size: 573 786 5122 Fragments arranged by size: 5122 786 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2021 3105 3499 3931 4692 5720 Size: 275 186 20 1084 394 432 761 1028 Cuts at: 5720 1540 Size: 2301 Fragments arranged by size: 2301 1084 1028 761 432 394 275 186 20 BsiHKAI G_wGCw'C Cuts at: 2625 3186 4542 4627 5698 2625 Size: 561 1356 85 1071 3408 Fragments arranged by size: 3408 1356 1071 561 85 BsiWI C'GTAC_G Cuts at: 2064 2064 Size: 6481 BsmI GAATG_Cn' Cuts at: 2175 3125 3992 4085 2175 Size: 950 867 93 4571 Fragments arranged by size: 4571 950 867 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3425 3756 3425 Size: 331 6150 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2625 3116 3186 3800 4542 4627 5698 Size: 399 835 491 70 614 742 85 1071 Cuts at: 5698 1391 Size: 2174 Fragments arranged by size: 2174 1071 835 742 614 491 399 85 70 BspEI T'CCGG_A Cuts at: 6144 6144 Size: 6481 BspHI T'CATG_A Cuts at: 4370 6100 4370 Size: 1730 4751 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2998 3294 3836 844 Size: 2154 296 542 3489 Fragments arranged by size: 3489 2154 542 296 BsrBI CCG'CTC Cuts at: 1394 1823 2176 4368 1394 Size: 429 353 2192 3507 Fragments arranged by size: 3507 2192 429 353 BsrDI GCAATG_nn' Cuts at: 65 1248 4976 5150 65 Size: 1183 3728 174 1396 Fragments arranged by size: 3728 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2512 3494 5125 5312 1700 Size: 110 702 982 1631 187 2869 Fragments arranged by size: 2869 1631 982 702 187 110 BsrGI T'GTAC_A Cuts at: 96 2679 96 Size: 2583 3898 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6481 BssSI C'ACGA_G Cuts at: 3641 4541 5553 3641 Size: 900 1012 4569 Fragments arranged by size: 4569 1012 900 BstAPI GCAn_nnn'nTGC Cuts at: 794 2209 794 Size: 1415 5066 BstBI TT'CG_AA Cuts at: 5285 5285 Size: 6481 BstEII G'GTnAC_C Cuts at: 1184 2393 1184 Size: 1209 5272 BstXI CCAn_nnnn'nTGG Cuts at: 2281 3001 3116 3340 2281 Size: 720 115 224 5422 Fragments arranged by size: 5422 720 224 115 BstYI r'GATC_y Cuts at: 1972 2359 2745 3811 4178 4563 4580 6032 6118 Size: 387 386 1066 367 385 17 1452 86 Cuts at: 6118 6130 6476 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1066 387 386 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2070 2369 2656 3774 513 Size: 552 939 66 299 287 1118 3220 Fragments arranged by size: 3220 1118 939 552 299 287 66 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5742 Fragments arranged by size: 5742 683 56 BtsI GCAGTG_nn' Cuts at: 2510 2606 3545 3997 4791 4811 2510 Size: 96 939 452 794 20 4180 Fragments arranged by size: 4180 939 794 452 96 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6481 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6446 DraI TTT'AAA Cuts at: 3785 3785 Size: 6481 DraIII CAC_nnn'GTG Cuts at: 3708 3708 Size: 6481 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3104 5488 809 Size: 507 1788 2384 1802 Fragments arranged by size: 2384 1802 1788 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2018 2709 3102 Size: 54 1425 50 275 18 188 691 393 Cuts at: 3102 3240 3396 3496 3777 3928 4817 6446 8 Size: 138 156 100 281 151 889 1629 43 Fragments arranged by size: 1629 1425 889 691 393 281 275 188 156 151 138 100 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2018 3102 3496 3928 1537 Size: 275 206 1084 394 432 4090 Fragments arranged by size: 4090 1084 432 394 275 206 EarI CTCTTCn'nnn_ Cuts at: 1353 2348 4411 1353 Size: 995 2063 3423 Fragments arranged by size: 3423 2063 995 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2136 5068 5442 5588 387 Size: 1064 138 547 2932 374 146 1280 Fragments arranged by size: 2932 1280 1064 547 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2254 2468 3023 3262 3626 4544 5141 Size: 393 622 214 555 239 364 918 597 Cuts at: 5141 5928 6196 1239 Size: 787 268 1524 Fragments arranged by size: 1524 918 787 622 597 555 393 364 268 239 214 EcoNI CCTnn'n_nnAGG Cuts at: 3005 3290 3005 Size: 285 6196 EcoO109I rG'GnC_Cy Cuts at: 2644 3600 2644 Size: 956 5525 EcoRI G'AATT_C Cuts at: 3790 4168 3790 Size: 378 6103 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2929 2929 Size: 6481 FspI TGC'GCA Cuts at: 4987 4987 Size: 6481 HaeII r_GCGC'y Cuts at: 1678 2232 2519 5628 1678 Size: 554 287 3109 2531 Fragments arranged by size: 3109 2531 554 287 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2414 2446 5122 5154 5196 Size: 32 135 32 842 32 2676 32 42 Cuts at: 5196 5228 1373 Size: 32 2626 Fragments arranged by size: 2676 2626 842 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3325 3825 4071 669 Size: 988 281 1387 500 246 3079 Fragments arranged by size: 3079 1387 988 500 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6481 HpaI GTT'AAC Cuts at: 4071 4071 Size: 6481 KasI G'GCGC_C Cuts at: 2228 2228 Size: 6481 KpnI G_GTAC'C Cuts at: 2341 3806 2341 Size: 1465 5016 MfeI C'AATT_G Cuts at: 4080 4080 Size: 6481 MluI A'CGCG_T Cuts at: 6357 6357 Size: 6481 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2835 5595 5779 1305 Size: 1530 2760 184 2007 Fragments arranged by size: 2760 2007 1530 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2711 10 Size: 54 1425 343 879 3780 Fragments arranged by size: 3780 1425 879 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2150 2681 2837 3161 3338 4439 4798 Size: 648 984 531 156 324 177 1101 359 Cuts at: 4798 4957 518 Size: 159 2042 Fragments arranged by size: 2042 1101 984 648 531 359 324 177 159 156 MspA1I CmG'CkG Cuts at: 1606 2023 2707 3172 3239 3897 4574 5722 5967 Size: 417 684 465 67 658 677 1148 245 Cuts at: 5967 1606 Size: 2120 Fragments arranged by size: 2120 1148 684 677 658 465 417 245 67 NaeI GCC'GGC Cuts at: 2514 2514 Size: 6481 NarI GG'CG_CC Cuts at: 2229 2229 Size: 6481 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4990 Fragments arranged by size: 4990 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6481 NgoMIV G'CCGG_C Cuts at: 2512 2512 Size: 6481 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6481 NotI GC'GGCC_GC Cuts at: 2018 3928 2018 Size: 1910 4571 NspI r_CATG'y Cuts at: 1132 1229 2212 2686 3226 3839 5384 1132 Size: 97 983 474 540 613 1545 2229 Fragments arranged by size: 2229 1545 983 613 540 474 97 PasI CC'CwG_GG Cuts at: 1348 3631 3727 1348 Size: 2283 96 4102 Fragments arranged by size: 4102 2283 96 PciI A'CATG_T Cuts at: 2682 5380 2682 Size: 2698 3783 PflMI CCAn_nnn'nTGG Cuts at: 1108 2390 2724 1108 Size: 1282 334 4865 Fragments arranged by size: 4865 1282 334 PfoI T'CCnGG_A Cuts at: 2355 2484 2355 Size: 129 6352 PmeI GTTT'AAAC Cuts at: 3785 3785 Size: 6481 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4550 4582 6063 6095 4550 Size: 32 1481 32 4936 Fragments arranged by size: 4936 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3600 3600 Size: 6481 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6481 PsiI TTA'TAA Cuts at: 4051 4051 Size: 6481 PstI C_TGCA'G Cuts at: 830 3080 3833 830 Size: 2250 753 3478 Fragments arranged by size: 3478 2250 753 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6481 PvuII CAG'CTG Cuts at: 2707 3172 2707 Size: 465 6016 SalI G'TCGA_C Cuts at: 1936 3823 1936 Size: 1887 4594 SanDI GG'GwC_CC Cuts at: 3600 3600 Size: 6481 SbfI CC_TGCA'GG Cuts at: 3833 3833 Size: 6481 ScaI AGT'ACT Cuts at: 2444 4729 6441 2444 Size: 2285 1712 2484 Fragments arranged by size: 2484 2285 1712 SfcI C'TryA_G Cuts at: 826 1045 2039 3076 3829 4964 5645 5836 826 Size: 219 994 1037 753 1135 681 191 1471 Fragments arranged by size: 1471 1135 1037 994 753 681 219 191 SfoI GGC'GCC Cuts at: 2230 2230 Size: 6481 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6481 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6481 SmaI CCC'GGG Cuts at: 1872 3808 1872 Size: 1936 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3482 3752 4585 5486 5748 820 Size: 197 926 1539 270 833 901 262 1553 Fragments arranged by size: 1553 1539 926 901 833 270 262 197 SnaBI TAC'GTA Cuts at: 493 4272 493 Size: 3779 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6481 SphI G_CATG'C Cuts at: 1132 2212 3226 3839 1132 Size: 1080 1014 613 3774 Fragments arranged by size: 3774 1080 1014 613 SspI AAT'ATT Cuts at: 5 52 4405 5 Size: 47 4353 2081 Fragments arranged by size: 4353 2081 47 StuI AGG'CCT Cuts at: 1517 2723 1517 Size: 1206 5275 StyI C'CwwG_G Cuts at: 513 1065 2004 2718 3291 3920 513 Size: 552 939 714 573 629 3074 Fragments arranged by size: 3074 939 714 629 573 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2756 4508 4525 4678 4863 5308 325 Size: 1255 1176 1752 17 153 185 445 1498 Fragments arranged by size: 1752 1498 1255 1176 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2304 2442 2679 Size: 274 80 33 51 1443 327 138 237 Cuts at: 2679 4727 6439 96 Size: 2048 1712 138 Fragments arranged by size: 2048 1712 1443 327 274 237 138 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6441 6473 6441 Size: 32 6449 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6481 XbaI T'CTAG_A Cuts at: 3817 3817 Size: 6481 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2584 3154 3307 2584 Size: 570 153 5758 Fragments arranged by size: 5758 570 153 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6481 XmaI C'CCGG_G Cuts at: 1870 3806 1870 Size: 1936 4545 XmnI GAAnn'nnTTC Cuts at: 4610 4610 Size: 6481 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4289 276 Size: 53 83 186 936 2755 2468 Fragments arranged by size: 2755 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SrfI SwaI