Restriction map for
pFN21AE6467
(Circular) (Six-base) MAPSORT of: pFN21AE6467.seq Check: 9090 from: 1 to: 6250
pFN21AE6467 6250 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4060 278
Size: 53 83 186 936 2524 2468
Fragments arranged by size:
2524 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2839 3593 1937
Size: 902 754 4594
Fragments arranged by size:
4594 902 754
Acc65I G'GTAC_C
Cuts at: 3571 3571
Size: 6250
AclI AA'CG_TT
Cuts at: 1563 4377 4750 1563
Size: 2814 373 3063
Fragments arranged by size:
3063 2814 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2286 4313 5697 5965 1632
Size: 654 2027 1384 268 1917
Fragments arranged by size:
2027 1917 1384 654 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6053
AflIII A'CryG_T
Cuts at: 1203 2437 5149 6126 1203
Size: 1234 2712 977 1327
Fragments arranged by size:
2712 1327 1234 977
AgeI A'CCGG_T
Cuts at: 5081 5081
Size: 6250
AhdI GACnn_n'nnGTC
Cuts at: 4979 4979
Size: 6250
AleI CACnn'nnGTG
Cuts at: 1166 3047 1166
Size: 1881 4369
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6250
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2898 2930 2898
Size: 32 6218
AlwNI CAG_nnn'CTG
Cuts at: 2603 3993 5565 2603
Size: 1390 1572 3288
Fragments arranged by size:
3288 1572 1390
ApaLI G'TGCA_C
Cuts at: 4307 5463 4307
Size: 1156 5094
ApoI r'AATT_y
Cuts at: 1493 2284 2334 2627 3240 3559 3785 3937 6046
Size: 791 50 293 613 319 226 152 2109
Cuts at: 6046 1493
Size: 1697
Fragments arranged by size:
2109 1697 791 613 319 293 226 152
50
AseI AT'TA_AT
Cuts at: 160 1032 2756 4804 160
Size: 872 1724 2048 1606
Fragments arranged by size:
2048 1724 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6250
AvaI C'yCGr_G
Cuts at: 1870 1943 3575 1870
Size: 73 1632 4545
Fragments arranged by size:
4545 1632 73
BamHI G'GATC_C
Cuts at: 3580 3947 3580
Size: 367 5883
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3571 5026 618
Size: 325 217 627 1784 1455 1842
Fragments arranged by size:
1842 1784 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3460 3569 1391
Size: 2069 109 4072
Fragments arranged by size:
4072 2069 109
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2454 2940 928
Size: 971 555 486 4238
Fragments arranged by size:
4238 971 555 486
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4441 4475 1903
Size: 34 50 34 2420 34 3678
Fragments arranged by size:
3678 2420 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4142 5352 4142
Size: 1210 5040
BclI T'GATC_A
Cuts at: 1548 3438 1548
Size: 1890 4360
BfrBI ATG'CAT
Cuts at: 2008 2008
Size: 6250
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4861 136
Size: 107 122 71 631 605 3189 1525
Fragments arranged by size:
3189 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6245 6245
Size: 6250
BlpI GC'TnA_GC
Cuts at: 3667 3667
Size: 6250
Bme1580I G_kGCm'C
Cuts at: 1790 4311 5467 1790
Size: 2521 1156 2573
Fragments arranged by size:
2573 2521 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6250
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3981 4939 442
Size: 416 53 83 399 24 2564 958 1753
Fragments arranged by size:
2564 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2769 1056
Size: 1713 4537
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3051 4910 1239
Size: 1812 1859 2579
Fragments arranged by size:
2579 1859 1812
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2833 4375 5240 5538 2833
Size: 1542 865 298 3545
Fragments arranged by size:
3545 1542 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4913 882
Size: 617 3414 2219
Fragments arranged by size:
3414 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4041 493
Size: 711 2837 2702
Fragments arranged by size:
2837 2702 711
BsaBI GATnn'nnATC
Cuts at: 6244 6244
Size: 6250
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4057 4439 5074 275
Size: 53 83 186 936 2524 382 635 1451
Fragments arranged by size:
2524 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4683 5081 5355 5502 5913 4683
Size: 398 274 147 411 5020
Fragments arranged by size:
5020 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3150 3180 1178
Size: 30 165 30 1747 30 4248
Fragments arranged by size:
4248 1747 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2058 2202 1186
Size: 872 144 5234
Fragments arranged by size:
5234 872 144
BseYI C'CCAG_C
Cuts at: 2507 5453 5949 2507
Size: 2946 496 2808
Fragments arranged by size:
2946 2808 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2082 2616 1163
Size: 573 346 534 4797
Fragments arranged by size:
4797 573 534 346
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3700 4461 5489 1540
Size: 275 186 1699 761 1028 2301
Fragments arranged by size:
2301 1699 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3460 4311 4396 5467 3460
Size: 851 85 1071 4243
Fragments arranged by size:
4243 1071 851 85
BsmI GAATG_Cn'
Cuts at: 2742 3471 3761 3854 2742
Size: 729 290 93 5138
Fragments arranged by size:
5138 729 290 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3460 3569 4311 4396 5467 1391
Size: 399 1670 109 742 85 1071 2174
Fragments arranged by size:
2174 1670 1071 742 399 109 85
BspEI T'CCGG_A
Cuts at: 5913 5913
Size: 6250
BspHI T'CATG_A
Cuts at: 4139 5869 4139
Size: 1730 4520
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3605 844
Size: 2761 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4137 1394
Size: 429 2314 3507
Fragments arranged by size:
3507 2314 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2478 2645 3060 4745 4919 65
Size: 1183 1230 167 415 1685 174 1396
Fragments arranged by size:
1685 1396 1230 1183 415 174 167
BsrFI r'CCGG_y
Cuts at: 1700 1810 4894 5081 1700
Size: 110 3084 187 2869
Fragments arranged by size:
3084 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6250
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6250
BssSI C'ACGA_G
Cuts at: 4310 5322 4310
Size: 1012 5238
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6250
BstBI TT'CG_AA
Cuts at: 3238 5054 3238
Size: 1816 4434
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6250
BstXI CCAn_nnnn'nTGG
Cuts at: 3014 3014
Size: 6250
BstYI r'GATC_y
Cuts at: 1972 3026 3580 3947 4332 4349 5801 5887 5899
Size: 1054 554 367 385 17 1452 86 12
Cuts at: 5899 6245 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1054 554 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2840 2840
Size: 6250
Bsu36I CC'TnA_GG
Cuts at: 2830 2830
Size: 6250
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5698
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3162 487
Size: 683 56 1936 3575
Fragments arranged by size:
3575 1936 683 56
BtsI GCAGTG_nn'
Cuts at: 3766 4560 4580 3766
Size: 794 20 5436
Fragments arranged by size:
5436 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6250
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2514 2549 529
Size: 35 1950 35 4230
Fragments arranged by size:
4230 1950 35 35
DraI TTT'AAA
Cuts at: 3554 3554
Size: 6250
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3076 5257 809
Size: 507 1760 2181 1802
Fragments arranged by size:
2181 1802 1760 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3697 4586 6215
Size: 54 1425 50 275 18 1867 889 1629
Cuts at: 6215 8
Size: 43
Fragments arranged by size:
1867 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3697 1537
Size: 275 1885 4090
Fragments arranged by size:
4090 1885 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2385 2951 4180 1353
Size: 1032 566 1229 3423
Fragments arranged by size:
3423 1229 1032 566
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4837 5211 5357 387
Size: 1064 138 3248 374 146 1280
Fragments arranged by size:
3248 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3458 3458
Size: 6250
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2286 3051 4313 4910 5697 5965 1239
Size: 393 654 765 1262 597 787 268 1524
Fragments arranged by size:
1524 1262 787 765 654 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2184 2585 2863 2184
Size: 401 278 5571
Fragments arranged by size:
5571 401 278
EcoO109I rG'GnC_Cy
Cuts at: 3347 3479 3347
Size: 132 6118
EcoRI G'AATT_C
Cuts at: 3559 3937 3559
Size: 378 5872
FspI TGC'GCA
Cuts at: 4756 4756
Size: 6250
HaeII r_GCGC'y
Cuts at: 1678 5397 1678
Size: 3719 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3430 3462 4891 4923 4965
Size: 32 135 32 1858 32 1429 32 42
Cuts at: 4965 4997 1373
Size: 32 2626
Fragments arranged by size:
2626 1858 1429 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3534 3594 3840 669
Size: 988 281 1596 60 246 3079
Fragments arranged by size:
3079 1596 988 281 246 60
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6250
HpaI GTT'AAC
Cuts at: 3840 3840
Size: 6250
KpnI G_GTAC'C
Cuts at: 3575 3575
Size: 6250
MfeI C'AATT_G
Cuts at: 3849 3849
Size: 6250
MluI A'CGCG_T
Cuts at: 6126 6126
Size: 6250
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2771 3136 5364 5548 1305
Size: 1466 365 2228 184 2007
Fragments arranged by size:
2228 2007 1466 365 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4428
Fragments arranged by size:
4428 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2442 2705 3047 4208 4567 4726 518
Size: 648 1276 263 342 1161 359 159 2042
Fragments arranged by size:
2042 1276 1161 648 359 342 263 159
MspA1I CmG'CkG
Cuts at: 1606 2600 3666 4343 5491 5736 1606
Size: 994 1066 677 1148 245 2120
Fragments arranged by size:
2120 1148 1066 994 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5698
NdeI CA'TA_TG
Cuts at: 387 3397 387
Size: 3010 3240
NheI G'CTAG_C
Cuts at: 1052 2765 1052
Size: 1713 4537
NotI GC'GGCC_GC
Cuts at: 3697 3697
Size: 6250
NsiI A_TGCA'T
Cuts at: 2010 2010
Size: 6250
NspI r_CATG'y
Cuts at: 1132 1229 2441 2811 3608 5153 1132
Size: 97 1212 370 797 1545 2229
Fragments arranged by size:
2229 1545 1212 797 370 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6250
PciI A'CATG_T
Cuts at: 2437 5149 2437
Size: 2712 3538
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6250
PmeI GTTT'AAAC
Cuts at: 3554 3554
Size: 6250
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4319 4351 5832 5864 4319
Size: 32 1481 32 4705
Fragments arranged by size:
4705 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3347 3347
Size: 6250
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6250
PsiI TTA'TAA
Cuts at: 3820 3820
Size: 6250
PstI C_TGCA'G
Cuts at: 830 3528 3602 830
Size: 2698 74 3478
Fragments arranged by size:
3478 2698 74
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6250
PvuII CAG'CTG
Cuts at: 2600 2600
Size: 6250
SacI G_AGCT'C
Cuts at: 3460 3460
Size: 6250
SalI G'TCGA_C
Cuts at: 1936 3592 1936
Size: 1656 4594
SbfI CC_TGCA'GG
Cuts at: 3602 3602
Size: 6250
ScaI AGT'ACT
Cuts at: 4498 6210 4498
Size: 1712 4538
SexAI A'CCwGG_T
Cuts at: 2246 2246
Size: 6250
SfcI C'TryA_G
Cuts at: 826 1045 2568 3327 3524 3598 4733 5414 5605
Size: 219 1523 759 197 74 1135 681 191
Cuts at: 5605 826
Size: 1471
Fragments arranged by size:
1523 1471 1135 759 681 219 197 191
74
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6250
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6250
SmaI CCC'GGG
Cuts at: 1872 3577 1872
Size: 1705 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2812 4354 5255 5517 820
Size: 197 926 869 1542 901 262 1553
Fragments arranged by size:
1553 1542 926 901 869 262 197
SnaBI TAC'GTA
Cuts at: 493 4041 493
Size: 3548 2702
SpeI A'CTAG_T
Cuts at: 152 3046 152
Size: 2894 3356
SphI G_CATG'C
Cuts at: 1132 3608 1132
Size: 2476 3774
SspI AAT'ATT
Cuts at: 5 52 3193 3297 4174 5
Size: 47 3141 104 877 2081
Fragments arranged by size:
3141 2081 877 104 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6250
StyI C'CwwG_G
Cuts at: 513 1065 3689 513
Size: 552 2624 3074
Fragments arranged by size:
3074 2624 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4277 4294 4447 4632 5077 325
Size: 1255 2697 17 153 185 445 1498
Fragments arranged by size:
2697 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3331 4496 6208
Size: 274 80 33 51 1443 1354 1165 1712
Cuts at: 6208 96
Size: 138
Fragments arranged by size:
1712 1443 1354 1165 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6210 6242 6210
Size: 32 6218
Tth111I GACn'n_nGTC
Cuts at: 1316 2248 1316
Size: 932 5318
XbaI T'CTAG_A
Cuts at: 3358 3586 3358
Size: 228 6022
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3220 3220
Size: 6250
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6250
XmaI C'CCGG_G
Cuts at: 1870 3575 1870
Size: 1705 4545
XmnI GAAnn'nnTTC
Cuts at: 2742 4379 2742
Size: 1637 4613
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4058 276
Size: 53 83 186 936 2524 2468
Fragments arranged by size:
2524 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI DraIII EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI
SfiI SfoI SrfI SwaI