Restriction map for
pFN21AE6465
(Circular) (Six-base) MAPSORT of: pFN21AE6465.seq Check: 6621 from: 1 to: 6322
pFN21AE6465 6322 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2177 4132 278
Size: 53 83 186 936 641 1955 2468
Fragments arranged by size:
2468 1955 936 641 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3665 1937
Size: 1728 4594
Acc65I G'GTAC_C
Cuts at: 3643 3643
Size: 6322
AclI AA'CG_TT
Cuts at: 1563 4449 4822 1563
Size: 2886 373 3063
Fragments arranged by size:
3063 2886 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4385 5769 6037 1632
Size: 2753 1384 268 1917
Fragments arranged by size:
2753 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6125
AflIII A'CryG_T
Cuts at: 1203 2517 2790 5221 6198 1203
Size: 1314 273 2431 977 1327
Fragments arranged by size:
2431 1327 1314 977 273
AgeI A'CCGG_T
Cuts at: 5153 5153
Size: 6322
AhdI GACnn_n'nnGTC
Cuts at: 5051 5051
Size: 6322
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6322
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6322
AlwNI CAG_nnn'CTG
Cuts at: 4065 5637 4065
Size: 1572 4750
ApaLI G'TGCA_C
Cuts at: 4379 5535 4379
Size: 1156 5166
ApoI r'AATT_y
Cuts at: 1493 2040 2094 2352 2436 2571 2997 3631 3857
Size: 547 54 258 84 135 426 634 226
Cuts at: 3857 4009 6118 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 634 547 426 258 226 152
135 84 54
AseI AT'TA_AT
Cuts at: 160 1032 4876 160
Size: 872 3844 1606
Fragments arranged by size:
3844 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6322
AvaI C'yCGr_G
Cuts at: 1870 1943 3647 1870
Size: 73 1704 4545
Fragments arranged by size:
4545 1704 73
BamHI G'GATC_C
Cuts at: 2277 2377 3652 4019 2277
Size: 100 1275 367 4580
Fragments arranged by size:
4580 1275 367 100
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2635 3643 5098 618
Size: 325 217 627 848 1008 1455 1842
Fragments arranged by size:
1842 1455 1008 848 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3641 1391
Size: 2250 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2861 928
Size: 971 962 4389
Fragments arranged by size:
4389 971 962
BbvCI CC'TCA_GC
Cuts at: 3441 3441
Size: 6322
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4513 4547 1903
Size: 34 50 34 2492 34 3678
Fragments arranged by size:
3678 2492 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4214 5424 4214
Size: 1210 5112
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6322
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4933 136
Size: 107 122 71 631 605 3261 1525
Fragments arranged by size:
3261 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6317 6317
Size: 6322
BlpI GC'TnA_GC
Cuts at: 3739 3739
Size: 6322
Bme1580I G_kGCm'C
Cuts at: 1790 2638 2822 4383 5539 1790
Size: 848 184 1561 1156 2573
Fragments arranged by size:
2573 1561 1156 848 184
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6322
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2383 4053 5011
Size: 416 53 83 399 24 966 1670 958
Cuts at: 5011 442
Size: 1753
Fragments arranged by size:
1753 1670 966 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6322
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3415 3415
Size: 6322
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4982 1239
Size: 3743 2579
Bpu10I CC'TnA_GC
Cuts at: 3441 3441
Size: 6322
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4447 5312 5610 4447
Size: 865 298 5159
Fragments arranged by size:
5159 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2918 4985 882
Size: 617 1419 2067 2219
Fragments arranged by size:
2219 2067 1419 617
BsaAI yAC'GTr
Cuts at: 493 1204 4113 493
Size: 711 2909 2702
Fragments arranged by size:
2909 2702 711
BsaBI GATnn'nnATC
Cuts at: 6316 6316
Size: 6322
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2174 4129 4511 5146
Size: 53 83 186 936 641 1955 382 635
Cuts at: 5146 275
Size: 1451
Fragments arranged by size:
1955 1451 936 641 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2280 2347 4755 5153 5427 5574 5985 2280
Size: 67 2408 398 274 147 411 2617
Fragments arranged by size:
2617 2408 411 398 274 147 67
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6097
Fragments arranged by size:
6097 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2864 3419 3458 3567 1186
Size: 1678 555 39 109 3941
Fragments arranged by size:
3941 1678 555 109 39
BseYI C'CCAG_C
Cuts at: 2254 2605 2717 2821 5525 6021 2254
Size: 351 112 104 2704 496 2555
Fragments arranged by size:
2704 2555 496 351 112 104
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2189 2750 1163
Size: 573 453 561 4735
Fragments arranged by size:
4735 573 561 453
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3772 4533 5561 1540
Size: 275 186 1771 761 1028 2301
Fragments arranged by size:
2301 1771 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4383 4468 5539 4383
Size: 85 1071 5166
Fragments arranged by size:
5166 1071 85
BsmI GAATG_Cn'
Cuts at: 3195 3833 3926 3195
Size: 638 93 5591
Fragments arranged by size:
5591 638 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2638 2822 3641 4383 4468 5539 1391
Size: 399 848 184 819 742 85 1071 2174
Fragments arranged by size:
2174 1071 848 819 742 399 184 85
BspEI T'CCGG_A
Cuts at: 5985 5985
Size: 6322
BspHI T'CATG_A
Cuts at: 2244 3027 4211 5941 2244
Size: 783 1184 1730 2625
Fragments arranged by size:
2625 1730 1184 783
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3677 844
Size: 2833 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4209 1394
Size: 429 2386 3507
Fragments arranged by size:
3507 2386 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2235 3174 3270 4817 4991 65
Size: 1183 987 939 96 1547 174 1396
Fragments arranged by size:
1547 1396 1183 987 939 174 96
BsrFI r'CCGG_y
Cuts at: 1700 1810 2164 4966 5153 1700
Size: 110 354 2802 187 2869
Fragments arranged by size:
2869 2802 354 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6322
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6322
BssSI C'ACGA_G
Cuts at: 4382 5394 4382
Size: 1012 5310
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2702 794
Size: 1908 4414
BstBI TT'CG_AA
Cuts at: 5126 5126
Size: 6322
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6322
BstXI CCAn_nnnn'nTGG
Cuts at: 2713 3097 2713
Size: 384 5938
BstYI r'GATC_y
Cuts at: 1972 2277 2377 2405 3207 3652 4019 4404 4421
Size: 305 100 28 802 445 367 385 17
Cuts at: 4421 5873 5959 5971 6317 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 802 445 385 367 346 305
100 86 28 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2076 2401 2614 513
Size: 552 1011 325 213 4221
Fragments arranged by size:
4221 1011 552 325 213
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5583
Fragments arranged by size:
5583 683 56
BtsI GCAGTG_nn'
Cuts at: 2206 2703 3838 4632 4652 2206
Size: 497 1135 794 20 3876
Fragments arranged by size:
3876 1135 794 497 20
ClaI AT'CG_AT
Cuts at: 1554 2112 1554
Size: 558 5764
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3201 3236 529
Size: 35 2637 35 3615
Fragments arranged by size:
3615 2637 35 35
DraI TTT'AAA
Cuts at: 2014 3626 2014
Size: 1612 4710
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5329 809
Size: 507 4013 1802
Fragments arranged by size:
4013 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2661 3769 4658
Size: 54 1425 50 275 18 831 1108 889
Cuts at: 4658 6287 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1108 889 831 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3769 1537
Size: 275 1957 4090
Fragments arranged by size:
4090 1957 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3567 3591 4252 1353
Size: 2214 24 661 3423
Fragments arranged by size:
3423 2214 661 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4909 5283 5429 387
Size: 1064 138 3320 374 146 1280
Fragments arranged by size:
3320 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4385 4982 5769 6037 1239
Size: 393 2753 597 787 268 1524
Fragments arranged by size:
2753 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2961 3566 2961
Size: 605 5717
EcoRI G'AATT_C
Cuts at: 2571 3631 4009 2571
Size: 1060 378 4884
Fragments arranged by size:
4884 1060 378
FspI TGC'GCA
Cuts at: 4828 4828
Size: 6322
HaeII r_GCGC'y
Cuts at: 1678 5469 1678
Size: 3791 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2478 2510 3566 3598 4963
Size: 32 135 32 906 32 1056 32 1365
Cuts at: 4963 4995 5037 5069 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1365 1056 906 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2317 3163 3666 3912 669
Size: 988 281 379 846 503 246 3079
Fragments arranged by size:
3079 988 846 503 379 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6322
HpaI GTT'AAC
Cuts at: 3912 3912
Size: 6322
KpnI G_GTAC'C
Cuts at: 3647 3647
Size: 6322
MfeI C'AATT_G
Cuts at: 2969 3921 2969
Size: 952 5370
MluI A'CGCG_T
Cuts at: 6198 6198
Size: 6322
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2858 5436 5620 1305
Size: 1553 2578 184 2007
Fragments arranged by size:
2578 2007 1553 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2663 10
Size: 54 1425 343 831 3669
Fragments arranged by size:
3669 1425 831 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2939 3200 3330 4280 4639 4798 518
Size: 648 1773 261 130 950 359 159 2042
Fragments arranged by size:
2042 1773 950 648 359 261 159 130
MspA1I CmG'CkG
Cuts at: 1606 3738 4415 5563 5808 1606
Size: 2132 677 1148 245 2120
Fragments arranged by size:
2132 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2401 2614 513
Size: 552 1336 213 4221
Fragments arranged by size:
4221 1336 552 213
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6322
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6322
NotI GC'GGCC_GC
Cuts at: 3769 3769
Size: 6322
NspI r_CATG'y
Cuts at: 1132 1229 2521 2773 2794 3680 5225 1132
Size: 97 1292 252 21 886 1545 2229
Fragments arranged by size:
2229 1545 1292 886 252 97 21
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6322
PciI A'CATG_T
Cuts at: 2517 2790 5221 2517
Size: 273 2431 3618
Fragments arranged by size:
3618 2431 273
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6322
PmeI GTTT'AAAC
Cuts at: 3626 3626
Size: 6322
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4391 4423 5904 5936 4391
Size: 32 1481 32 4777
Fragments arranged by size:
4777 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6322
PsiI TTA'TAA
Cuts at: 3892 3892
Size: 6322
PstI C_TGCA'G
Cuts at: 830 2536 3674 830
Size: 1706 1138 3478
Fragments arranged by size:
3478 1706 1138
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6322
SalI G'TCGA_C
Cuts at: 1936 3664 1936
Size: 1728 4594
SapI GCTCTTCn'nnn_
Cuts at: 3567 3567
Size: 6322
SbfI CC_TGCA'GG
Cuts at: 3674 3674
Size: 6322
ScaI AGT'ACT
Cuts at: 4570 6282 4570
Size: 1712 4610
SfcI C'TryA_G
Cuts at: 826 1045 2532 3123 3670 4805 5486 5677 826
Size: 219 1487 591 547 1135 681 191 1471
Fragments arranged by size:
1487 1471 1135 681 591 547 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6322
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6322
SmaI CCC'GGG
Cuts at: 1872 3649 1872
Size: 1777 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4426 5327 5589 820
Size: 197 926 2483 901 262 1553
Fragments arranged by size:
2483 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4113 493
Size: 3620 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6322
SphI G_CATG'C
Cuts at: 1132 3680 1132
Size: 2548 3774
SspI AAT'ATT
Cuts at: 5 52 4246 5
Size: 47 4194 2081
Fragments arranged by size:
4194 2081 47
StuI AGG'CCT
Cuts at: 1517 2810 1517
Size: 1293 5029
StyI C'CwwG_G
Cuts at: 513 1065 2401 2614 3761 513
Size: 552 1336 213 1147 3074
Fragments arranged by size:
3074 1336 1147 552 213
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4349 4366 4519 4704 5149 325
Size: 1255 2769 17 153 185 445 1498
Fragments arranged by size:
2769 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2733 4568 6280
Size: 274 80 33 51 1443 756 1835 1712
Cuts at: 6280 96
Size: 138
Fragments arranged by size:
1835 1712 1443 756 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2151 2183 6282 6314 2151
Size: 32 4099 32 2159
Fragments arranged by size:
4099 2159 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6322
XbaI T'CTAG_A
Cuts at: 3658 3658
Size: 6322
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2308 3199 2308
Size: 891 5431
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6322
XmaI C'CCGG_G
Cuts at: 1870 3647 1870
Size: 1777 4545
XmnI GAAnn'nnTTC
Cuts at: 2040 3302 4451 2040
Size: 1262 1149 3911
Fragments arranged by size:
3911 1262 1149
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2175 4130 276
Size: 53 83 186 936 641 1955 2468
Fragments arranged by size:
2468 1955 936 641 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI