Restriction map for
pFN21AE6458
(Circular) (Six-base) MAPSORT of: pFN21AE6458.seq Check: 8288 from: 1 to: 6061
pFN21AE6458 6061 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3871 278
Size: 53 83 186 936 2335 2468
Fragments arranged by size:
2468 2335 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2843 3404 1937
Size: 906 561 4594
Fragments arranged by size:
4594 906 561
Acc65I G'GTAC_C
Cuts at: 3382 3382
Size: 6061
AclI AA'CG_TT
Cuts at: 1563 4188 4561 1563
Size: 2625 373 3063
Fragments arranged by size:
3063 2625 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2210 2406 2437 2462 3182 3215 3350 4124
Size: 578 196 31 25 720 33 135 774
Cuts at: 4124 5508 5776 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 774 720 578 268 196 135
33 31 25
AflII C'TTAA_G
Cuts at: 820 1017 2929 820
Size: 197 1912 3952
Fragments arranged by size:
3952 1912 197
AflIII A'CryG_T
Cuts at: 1203 2154 4960 5937 1203
Size: 951 2806 977 1327
Fragments arranged by size:
2806 1327 977 951
AgeI A'CCGG_T
Cuts at: 4892 4892
Size: 6061
AhdI GACnn_n'nnGTC
Cuts at: 4790 4790
Size: 6061
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6061
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6061
AlwNI CAG_nnn'CTG
Cuts at: 2084 3804 5376 2084
Size: 1720 1572 2769
Fragments arranged by size:
2769 1720 1572
ApaI G_GGCC'C
Cuts at: 2283 2283
Size: 6061
ApaLI G'TGCA_C
Cuts at: 4118 5274 4118
Size: 1156 4905
ApoI r'AATT_y
Cuts at: 1493 2468 2864 3182 3370 3596 3748 5857 1493
Size: 975 396 318 188 226 152 2109 1697
Fragments arranged by size:
2109 1697 975 396 318 226 188 152
AseI AT'TA_AT
Cuts at: 160 1032 4615 160
Size: 872 3583 1606
Fragments arranged by size:
3583 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
AvaI C'yCGr_G
Cuts at: 1870 1943 3386 1870
Size: 73 1443 4545
Fragments arranged by size:
4545 1443 73
BamHI G'GATC_C
Cuts at: 3391 3758 3391
Size: 367 5694
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2390 3382 4837 618
Size: 325 217 627 603 992 1455 1842
Fragments arranged by size:
1842 1455 992 627 603 325 217
BanII G_rGCy'C
Cuts at: 1391 2283 2739 3380 1391
Size: 892 456 641 4072
Fragments arranged by size:
4072 892 641 456
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2450 2679 928
Size: 971 551 229 4310
Fragments arranged by size:
4310 971 551 229
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4252 4286 1903
Size: 34 2315 34 3678
Fragments arranged by size:
3678 2315 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3953 5163 3953
Size: 1210 4851
BclI T'GATC_A
Cuts at: 1548 2778 1548
Size: 1230 4831
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4672 136
Size: 107 122 71 631 605 3000 1525
Fragments arranged by size:
3000 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6056 6056
Size: 6061
BlpI GC'TnA_GC
Cuts at: 3478 3478
Size: 6061
Bme1580I G_kGCm'C
Cuts at: 1790 2283 2363 4122 5278 1790
Size: 493 80 1759 1156 2573
Fragments arranged by size:
2573 1759 1156 493 80
BmgBI CAC'GTC
Cuts at: 1318 2841 1318
Size: 1523 4538
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2373 3792 4750
Size: 416 53 83 399 24 956 1419 958
Cuts at: 4750 442
Size: 1753
Fragments arranged by size:
1753 1419 958 956 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6061
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2306 3095 4721 1239
Size: 1067 789 1626 2579
Fragments arranged by size:
2579 1626 1067 789
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4186 5051 5349 4186
Size: 865 298 4898
Fragments arranged by size:
4898 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4724 882
Size: 617 3225 2219
Fragments arranged by size:
3225 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2905 3852 493
Size: 711 1701 947 2702
Fragments arranged by size:
2702 1701 947 711
BsaBI GATnn'nnATC
Cuts at: 6055 6055
Size: 6061
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3868 4250 4885 275
Size: 53 83 186 936 2335 382 635 1451
Fragments arranged by size:
2335 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4494 4892 5166 5313 5724 4494
Size: 398 274 147 411 4831
Fragments arranged by size:
4831 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5836
Fragments arranged by size:
5836 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2120 3044 1186
Size: 934 924 4203
Fragments arranged by size:
4203 934 924
BseYI C'CCAG_C
Cuts at: 2065 5264 5760 2065
Size: 3199 496 2366
Fragments arranged by size:
3199 2366 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5488
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3511 4272 5300 1540
Size: 275 186 1510 761 1028 2301
Fragments arranged by size:
2301 1510 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2634 4122 4207 5278 2634
Size: 1488 85 1071 3417
Fragments arranged by size:
3417 1488 1071 85
BsmI GAATG_Cn'
Cuts at: 2725 3572 3665 2725
Size: 847 93 5121
Fragments arranged by size:
5121 847 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2283 2363 2634 2739 3380 4122 4207
Size: 399 493 80 271 105 641 742 85
Cuts at: 4207 5278 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 641 493 399 271 105
85 80
BspEI T'CCGG_A
Cuts at: 5724 5724
Size: 6061
BspHI T'CATG_A
Cuts at: 2940 3039 3950 5680 2940
Size: 99 911 1730 3321
Fragments arranged by size:
3321 1730 911 99
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3416 844
Size: 2572 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3948 1394
Size: 429 2125 3507
Fragments arranged by size:
3507 2125 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4556 4730 65
Size: 1183 3308 174 1396
Fragments arranged by size:
3308 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4705 4892 1700
Size: 110 2895 187 2869
Fragments arranged by size:
2895 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2652 3210 96
Size: 2556 558 2947
Fragments arranged by size:
2947 2556 558
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6061
BssSI C'ACGA_G
Cuts at: 4121 5133 4121
Size: 1012 5049
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6061
BstBI TT'CG_AA
Cuts at: 4865 4865
Size: 6061
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6061
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2407 2011
Size: 396 5665
BstYI r'GATC_y
Cuts at: 1972 2610 3115 3391 3758 4143 4160 5612 5698
Size: 638 505 276 367 385 17 1452 86
Cuts at: 5698 5710 6056 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 638 505 385 367 346 276
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2979 2979
Size: 6061
BtgI C'CryG_G
Cuts at: 513 1065 2004 2036 2303 2355 3132 513
Size: 552 939 32 267 52 777 3442
Fragments arranged by size:
3442 939 777 552 267 52 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5322
Fragments arranged by size:
5322 683 56
BtsI GCAGTG_nn'
Cuts at: 2015 3577 4371 4391 2015
Size: 1562 794 20 3685
Fragments arranged by size:
3685 1562 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6061
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2867 2902 529
Size: 35 2303 35 3688
Fragments arranged by size:
3688 2303 35 35
DraI TTT'AAA
Cuts at: 3365 3365
Size: 6061
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5068 809
Size: 507 3752 1802
Fragments arranged by size:
3752 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3167 3508 4397
Size: 54 1425 50 275 18 1337 341 889
Cuts at: 4397 6026 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1337 889 341 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3508 1537
Size: 275 1696 4090
Fragments arranged by size:
4090 1696 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2670 3991 1353
Size: 1317 1321 3423
Fragments arranged by size:
3423 1321 1317
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4648 5022 5168 387
Size: 1064 138 3059 374 146 1280
Fragments arranged by size:
3059 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2210 2306 2406 2437 2462 3095 3182
Size: 393 578 96 100 31 25 633 87
Cuts at: 3182 3215 3350 4124 4721 5508 5776 1239
Size: 33 135 774 597 787 268 1524
Fragments arranged by size:
1524 787 774 633 597 578 393 268
135 100 96 87 33 31 25
EcoO109I rG'GnC_Cy
Cuts at: 2280 2404 2642 3205 2280
Size: 124 238 563 5136
Fragments arranged by size:
5136 563 238 124
EcoRI G'AATT_C
Cuts at: 2468 2864 3370 3748 2468
Size: 396 506 378 4781
Fragments arranged by size:
4781 506 396 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2467 2596 2467
Size: 129 5932
FspI TGC'GCA
Cuts at: 4567 4567
Size: 6061
HaeII r_GCGC'y
Cuts at: 1678 2026 2902 5208 1678
Size: 348 876 2306 2531
Fragments arranged by size:
2531 2306 876 348
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2553 2585 2972 3004 3209
Size: 32 135 32 981 32 387 32 205
Cuts at: 3209 3241 4702 4734 4776 4808 1373
Size: 32 1461 32 42 32 2626
Fragments arranged by size:
2626 1461 981 387 205 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3405 3651 669
Size: 988 281 1467 246 3079
Fragments arranged by size:
3079 1467 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6061
HpaI GTT'AAC
Cuts at: 3651 3651
Size: 6061
KpnI G_GTAC'C
Cuts at: 3386 3386
Size: 6061
MfeI C'AATT_G
Cuts at: 3660 3660
Size: 6061
MluI A'CGCG_T
Cuts at: 5937 5937
Size: 6061
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5175 5359 1305
Size: 3870 184 2007
Fragments arranged by size:
3870 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3169 10
Size: 54 1425 343 1337 2902
Fragments arranged by size:
2902 1425 1337 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4019 4378 4537 518
Size: 648 2853 359 159 2042
Fragments arranged by size:
2853 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2038 2059 2525 3112 3193 3477 4154 5302
Size: 432 21 466 587 81 284 677 1148
Cuts at: 5302 5547 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 587 466 432 284 245
81 21
NcoI C'CATG_G
Cuts at: 513 1065 2004 3132 513
Size: 552 939 1128 3442
Fragments arranged by size:
3442 1128 939 552
NdeI CA'TA_TG
Cuts at: 387 3297 387
Size: 2910 3151
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6061
NotI GC'GGCC_GC
Cuts at: 3508 3508
Size: 6061
NspI r_CATG'y
Cuts at: 1132 1229 2158 2662 3419 4964 1132
Size: 97 929 504 757 1545 2229
Fragments arranged by size:
2229 1545 929 757 504 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6061
PciI A'CATG_T
Cuts at: 2154 4960 2154
Size: 2806 3255
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6061
PfoI T'CCnGG_A
Cuts at: 2606 2606
Size: 6061
PmeI GTTT'AAAC
Cuts at: 3365 3365
Size: 6061
PmlI CAC'GTG
Cuts at: 2905 2905
Size: 6061
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4130 4162 5643 5675 4130
Size: 32 1481 32 4516
Fragments arranged by size:
4516 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2404 2642 2404
Size: 238 5823
PshAI GACnn'nnGTC
Cuts at: 1613 2679 1613
Size: 1066 4995
PsiI TTA'TAA
Cuts at: 2547 3631 2547
Size: 1084 4977
PspOMI G'GGCC_C
Cuts at: 2279 2279
Size: 6061
PstI C_TGCA'G
Cuts at: 830 3413 830
Size: 2583 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6061
PvuII CAG'CTG
Cuts at: 2525 3112 3193 2525
Size: 587 81 5393
Fragments arranged by size:
5393 587 81
RsrII CG'GwC_CG
Cuts at: 2137 2137
Size: 6061
SacII CC_GC'GG
Cuts at: 2039 2039
Size: 6061
SalI G'TCGA_C
Cuts at: 1936 3403 1936
Size: 1467 4594
SbfI CC_TGCA'GG
Cuts at: 3413 3413
Size: 6061
ScaI AGT'ACT
Cuts at: 2715 4309 6021 2715
Size: 1594 1712 2755
Fragments arranged by size:
2755 1712 1594
SfcI C'TryA_G
Cuts at: 826 1045 2740 3409 4544 5225 5416 826
Size: 219 1695 669 1135 681 191 1471
Fragments arranged by size:
1695 1471 1135 681 669 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6061
SmaI CCC'GGG
Cuts at: 1872 3388 1872
Size: 1516 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2929 4165 5066 5328 820
Size: 197 926 986 1236 901 262 1553
Fragments arranged by size:
1553 1236 986 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3852 493
Size: 3359 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6061
SphI G_CATG'C
Cuts at: 1132 3419 1132
Size: 2287 3774
SspI AAT'ATT
Cuts at: 5 52 3985 5
Size: 47 3933 2081
Fragments arranged by size:
3933 2081 47
StuI AGG'CCT
Cuts at: 1517 3146 1517
Size: 1629 4432
StyI C'CwwG_G
Cuts at: 513 1065 2004 2400 2595 3132 3313 3500 513
Size: 552 939 396 195 537 181 187 3074
Fragments arranged by size:
3074 939 552 537 396 195 187 181
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2588 3139 4088 4105 4258 4443 4888
Size: 1255 1008 551 949 17 153 185 445
Cuts at: 4888 325
Size: 1498
Fragments arranged by size:
1498 1255 1008 949 551 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2652 2713 3210
Size: 274 80 33 51 1443 675 61 497
Cuts at: 3210 4307 6019 96
Size: 1097 1712 138
Fragments arranged by size:
1712 1443 1097 675 497 274 138 80
61 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6021 6053 6021
Size: 32 6029
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6061
XbaI T'CTAG_A
Cuts at: 2536 2860 3397 2536
Size: 324 537 5200
Fragments arranged by size:
5200 537 324
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2845 2942 2845
Size: 97 5964
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6061
XmaI C'CCGG_G
Cuts at: 1870 3386 1870
Size: 1516 4545
XmnI GAAnn'nnTTC
Cuts at: 2447 4190 2447
Size: 1743 4318
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3869 276
Size: 53 83 186 936 2335 2468
Fragments arranged by size:
2468 2335 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII RsrII SacII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PspXI PsrI SacI SanDI SapI
SexAI SfiI SfoI SrfI SwaI