Restriction map for
pFN21AE6452
(Circular) (Six-base) MAPSORT of: pFN21AE6452.seq Check: 7738 from: 1 to: 6163
pFN21AE6452 6163 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3973 278
Size: 53 83 186 936 2437 2468
Fragments arranged by size:
2468 2437 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2030 3156 3506 1937
Size: 93 1126 350 4594
Fragments arranged by size:
4594 1126 350 93
Acc65I G'GTAC_C
Cuts at: 3484 3484
Size: 6163
AclI AA'CG_TT
Cuts at: 1563 4290 4663 1563
Size: 2727 373 3063
Fragments arranged by size:
3063 2727 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2348 2401 2969 4226 5610 5878 1632
Size: 716 53 568 1257 1384 268 1917
Fragments arranged by size:
1917 1384 1257 716 568 268 53
AfeI AGC'GCT
Cuts at: 3144 3144
Size: 6163
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5966
AflIII A'CryG_T
Cuts at: 1203 5062 6039 1203
Size: 3859 977 1327
Fragments arranged by size:
3859 1327 977
AgeI A'CCGG_T
Cuts at: 4994 4994
Size: 6163
AhdI GACnn_n'nnGTC
Cuts at: 4892 4892
Size: 6163
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6163
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2725 3351 1184
Size: 1541 626 3996
Fragments arranged by size:
3996 1541 626
AlwNI CAG_nnn'CTG
Cuts at: 3446 3906 5478 3446
Size: 460 1572 4131
Fragments arranged by size:
4131 1572 460
ApaLI G'TGCA_C
Cuts at: 4220 5376 4220
Size: 1156 5007
ApoI r'AATT_y
Cuts at: 1493 3472 3698 3850 5959 1493
Size: 1979 226 152 2109 1697
Fragments arranged by size:
2109 1979 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4717 160
Size: 872 3685 1606
Fragments arranged by size:
3685 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6163
AvaI C'yCGr_G
Cuts at: 1870 1943 3488 1870
Size: 73 1545 4545
Fragments arranged by size:
4545 1545 73
BamHI G'GATC_C
Cuts at: 2181 3493 3860 2181
Size: 1312 367 4484
Fragments arranged by size:
4484 1312 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2471 2576 2827 3077 3484
Size: 325 217 627 684 105 251 250 407
Cuts at: 3484 4939 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 684 627 407 325 251 250
217 105
BanII G_rGCy'C
Cuts at: 1391 2026 2312 3000 3191 3482 1391
Size: 635 286 688 191 291 4072
Fragments arranged by size:
4072 688 635 291 286 191
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2661 928
Size: 971 762 4430
Fragments arranged by size:
4430 971 762
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4354 4388 1903
Size: 34 2417 34 3678
Fragments arranged by size:
3678 2417 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4055 5265 4055
Size: 1210 4953
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6163
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4774 136
Size: 107 122 71 631 605 3102 1525
Fragments arranged by size:
3102 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6158 6158
Size: 6163
BlpI GC'TnA_GC
Cuts at: 3580 3580
Size: 6163
Bme1580I G_kGCm'C
Cuts at: 1790 2704 4224 5380 1790
Size: 914 1520 1156 2573
Fragments arranged by size:
2573 1520 1156 914
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6163
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3092 3316 3894
Size: 416 53 83 399 24 1675 224 578
Cuts at: 3894 4852 442
Size: 958 1753
Fragments arranged by size:
1753 1675 958 578 416 399 224 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6163
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2552 2552
Size: 6163
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2025 2373 2604 4823 1239
Size: 786 348 231 2219 2579
Fragments arranged by size:
2579 2219 786 348 231
Bpu10I CC'TnA_GC
Cuts at: 2306 3354 2306
Size: 1048 5115
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4288 5153 5451 4288
Size: 865 298 5000
Fragments arranged by size:
5000 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4826 882
Size: 617 3327 2219
Fragments arranged by size:
3327 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3954 493
Size: 711 2750 2702
Fragments arranged by size:
2750 2702 711
BsaBI GATnn'nnATC
Cuts at: 6157 6157
Size: 6163
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3970 4352 4987 275
Size: 53 83 186 936 2437 382 635 1451
Fragments arranged by size:
2437 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4596 4994 5268 5415 5826 4596
Size: 398 274 147 411 4933
Fragments arranged by size:
4933 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2023 2053 1178
Size: 30 165 30 620 30 5288
Fragments arranged by size:
5288 620 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3236 1186
Size: 2050 4113
BseYI C'CCAG_C
Cuts at: 2052 2272 5366 5862 2052
Size: 220 3094 496 2353
Fragments arranged by size:
3094 2353 496 220
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2080 1163
Size: 573 344 5246
Fragments arranged by size:
5246 573 344
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3613 4374 5402 1540
Size: 275 186 1612 761 1028 2301
Fragments arranged by size:
2301 1612 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2026 2221 2312 4224 4309 5380 2026
Size: 195 91 1912 85 1071 2809
Fragments arranged by size:
2809 1912 1071 195 91 85
BsmI GAATG_Cn'
Cuts at: 3239 3674 3767 3239
Size: 435 93 5635
Fragments arranged by size:
5635 435 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2782 2782
Size: 6163
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2026 2221 2312 2704 3000 3191 3482
Size: 399 236 195 91 392 296 191 291
Cuts at: 3482 4224 4309 5380 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 392 296 291 236
195 191 91 85
BspEI T'CCGG_A
Cuts at: 5826 5826
Size: 6163
BspHI T'CATG_A
Cuts at: 4052 5782 4052
Size: 1730 4433
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3518 844
Size: 2674 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4050 1394
Size: 429 2227 3507
Fragments arranged by size:
3507 2227 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3333 4658 4832 65
Size: 1183 2085 1325 174 1396
Fragments arranged by size:
2085 1396 1325 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3221 4807 4994 1700
Size: 110 1411 1586 187 2869
Fragments arranged by size:
2869 1586 1411 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6163
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6163
BssSI C'ACGA_G
Cuts at: 2320 3041 4223 5235 2320
Size: 721 1182 1012 3248
Fragments arranged by size:
3248 1182 1012 721
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6163
BstBI TT'CG_AA
Cuts at: 4967 4967
Size: 6163
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6163
BstYI r'GATC_y
Cuts at: 1972 2181 3493 3860 4245 4262 5714 5800 5812
Size: 209 1312 367 385 17 1452 86 12
Cuts at: 5812 6158 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1312 385 367 346 209 86
17 12
BstZ17I GTA'TAC
Cuts at: 3157 3157
Size: 6163
BtgI C'CryG_G
Cuts at: 513 1065 2004 2400 2982 513
Size: 552 939 396 582 3694
Fragments arranged by size:
3694 939 582 552 396
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3181 487
Size: 683 56 1955 3469
Fragments arranged by size:
3469 1955 683 56
BtsI GCAGTG_nn'
Cuts at: 3177 3231 3447 3679 4473 4493 3177
Size: 54 216 232 794 20 4847
Fragments arranged by size:
4847 794 232 216 54 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6163
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6128
DraI TTT'AAA
Cuts at: 3467 3467
Size: 6163
DraIII CAC_nnn'GTG
Cuts at: 3172 3172
Size: 6163
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5170 809
Size: 507 3854 1802
Fragments arranged by size:
3854 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3610 4499 6128
Size: 54 1425 50 275 18 1780 889 1629
Cuts at: 6128 8
Size: 43
Fragments arranged by size:
1780 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3610 1537
Size: 275 1798 4090
Fragments arranged by size:
4090 1798 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4093 1353
Size: 2740 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4750 5124 5270 387
Size: 1064 138 3161 374 146 1280
Fragments arranged by size:
3161 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2024 2310 2024
Size: 286 5877
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2025 2348 2373 2401 2604 2969 4226
Size: 393 393 323 25 28 203 365 1257
Cuts at: 4226 4823 5610 5878 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1257 787 597 393 393 365 323
268 203 28 25
EcoNI CCTnn'n_nnAGG
Cuts at: 2584 2584
Size: 6163
EcoRI G'AATT_C
Cuts at: 3472 3850 3472
Size: 378 5785
FspI TGC'GCA
Cuts at: 4669 4669
Size: 6163
HaeII r_GCGC'y
Cuts at: 1678 3146 5310 1678
Size: 1468 2164 2531
Fragments arranged by size:
2531 2164 1468
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4804 4836 4878 4910 1373
Size: 32 135 32 3232 32 42 32 2626
Fragments arranged by size:
3232 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3507 3753 669
Size: 988 281 1569 246 3079
Fragments arranged by size:
3079 1569 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6163
HpaI GTT'AAC
Cuts at: 3753 3753
Size: 6163
KpnI G_GTAC'C
Cuts at: 3488 3488
Size: 6163
MfeI C'AATT_G
Cuts at: 3762 3762
Size: 6163
MluI A'CGCG_T
Cuts at: 6039 6039
Size: 6163
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2827 5277 5461 1305
Size: 1522 2450 184 2007
Fragments arranged by size:
2450 2007 1522 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4341
Fragments arranged by size:
4341 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2923 4121 4480 4639 518
Size: 648 1757 1198 359 159 2042
Fragments arranged by size:
2042 1757 1198 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3579 4256 5404 5649 1606
Size: 1973 677 1148 245 2120
Fragments arranged by size:
2120 1973 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2400 513
Size: 552 939 396 4276
Fragments arranged by size:
4276 939 552 396
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6163
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6163
NotI GC'GGCC_GC
Cuts at: 3610 3610
Size: 6163
NspI r_CATG'y
Cuts at: 1132 1229 3521 5066 1132
Size: 97 2292 1545 2229
Fragments arranged by size:
2292 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6163
PciI A'CATG_T
Cuts at: 5062 5062
Size: 6163
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2697 1108
Size: 1589 4574
PmeI GTTT'AAAC
Cuts at: 3467 3467
Size: 6163
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4232 4264 5745 5777 4232
Size: 32 1481 32 4618
Fragments arranged by size:
4618 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6163
PsiI TTA'TAA
Cuts at: 3302 3733 3302
Size: 431 5732
PstI C_TGCA'G
Cuts at: 830 2807 3238 3363 3515 830
Size: 1977 431 125 152 3478
Fragments arranged by size:
3478 1977 431 152 125
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6163
SacI G_AGCT'C
Cuts at: 2026 2312 2026
Size: 286 5877
SalI G'TCGA_C
Cuts at: 1936 3505 1936
Size: 1569 4594
SbfI CC_TGCA'GG
Cuts at: 3515 3515
Size: 6163
ScaI AGT'ACT
Cuts at: 2414 4411 6123 2414
Size: 1997 1712 2454
Fragments arranged by size:
2454 1997 1712
SfcI C'TryA_G
Cuts at: 826 1045 2803 3234 3359 3435 3451 3511 4646
Size: 219 1758 431 125 76 16 60 1135
Cuts at: 4646 5327 5518 826
Size: 681 191 1471
Fragments arranged by size:
1758 1471 1135 681 431 219 191 125
76 60 16
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6163
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6163
SmaI CCC'GGG
Cuts at: 1872 3490 1872
Size: 1618 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4267 5168 5430 820
Size: 197 926 2324 901 262 1553
Fragments arranged by size:
2324 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3954 493
Size: 3461 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6163
SphI G_CATG'C
Cuts at: 1132 3521 1132
Size: 2389 3774
SspI AAT'ATT
Cuts at: 5 52 4087 5
Size: 47 4035 2081
Fragments arranged by size:
4035 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6163
StyI C'CwwG_G
Cuts at: 513 1065 2004 2400 3602 513
Size: 552 939 396 1202 3074
Fragments arranged by size:
3074 1202 939 552 396
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2016 2264 2948 3208 4190 4207 4360
Size: 1255 436 248 684 260 982 17 153
Cuts at: 4360 4545 4990 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 982 684 445 436 260 248
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2412 2736 2820
Size: 274 80 33 51 1443 435 324 84
Cuts at: 2820 4409 6121 96
Size: 1589 1712 138
Fragments arranged by size:
1712 1589 1443 435 324 274 138 84
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6123 6155 6123
Size: 32 6131
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6163
XbaI T'CTAG_A
Cuts at: 3499 3499
Size: 6163
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2049 3115 3379 2049
Size: 1066 264 4833
Fragments arranged by size:
4833 1066 264
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6163
XmaI C'CCGG_G
Cuts at: 1870 3488 1870
Size: 1618 4545
XmnI GAAnn'nnTTC
Cuts at: 4292 4292
Size: 6163
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3971 276
Size: 53 83 186 936 2437 2468
Fragments arranged by size:
2468 2437 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BstXI Bsu36I EcoO109I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI