Restriction map for
pFN21AE6449
(Circular) (Six-base) MAPSORT of: pFN21AE6449.seq Check: 9875 from: 1 to: 6307
pFN21AE6449 6307 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2433 3435 2433
Size: 1002 5305
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4117 278
Size: 53 83 186 936 2581 2468
Fragments arranged by size:
2581 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3650 1937
Size: 1713 4594
Acc65I G'GTAC_C
Cuts at: 3456 3628 3456
Size: 172 6135
AclI AA'CG_TT
Cuts at: 1563 4434 4807 1563
Size: 2871 373 3063
Fragments arranged by size:
3063 2871 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3288 4370 5754 6022 1632
Size: 1656 1082 1384 268 1917
Fragments arranged by size:
1917 1656 1384 1082 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6110
AflIII A'CryG_T
Cuts at: 1203 5206 6183 1203
Size: 4003 977 1327
Fragments arranged by size:
4003 1327 977
AgeI A'CCGG_T
Cuts at: 5138 5138
Size: 6307
AhdI GACnn_n'nnGTC
Cuts at: 5036 5036
Size: 6307
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6307
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2445 1184
Size: 1261 5046
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2007 2039 2007
Size: 32 6275
AlwNI CAG_nnn'CTG
Cuts at: 2124 4050 5622 2124
Size: 1926 1572 2809
Fragments arranged by size:
2809 1926 1572
ApaLI G'TGCA_C
Cuts at: 4364 5520 4364
Size: 1156 5151
ApoI r'AATT_y
Cuts at: 1493 2267 2330 2873 2946 3616 3842 3994 6103
Size: 774 63 543 73 670 226 152 2109
Cuts at: 6103 1493
Size: 1697
Fragments arranged by size:
2109 1697 774 670 543 226 152 73
63
AscI GG'CGCG_CC
Cuts at: 3417 3417
Size: 6307
AseI AT'TA_AT
Cuts at: 160 1032 2889 4861 160
Size: 872 1857 1972 1606
Fragments arranged by size:
1972 1857 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6307
AvaI C'yCGr_G
Cuts at: 1870 1943 3632 1870
Size: 73 1689 4545
Fragments arranged by size:
4545 1689 73
BamHI G'GATC_C
Cuts at: 3637 4004 3637
Size: 367 5940
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2104 2421 3456 3628 5083
Size: 325 217 627 317 317 1035 172 1455
Cuts at: 5083 618
Size: 1842
Fragments arranged by size:
1842 1455 1035 627 325 317 317 217
172
BanII G_rGCy'C
Cuts at: 1391 3626 1391
Size: 2235 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2818 928
Size: 971 919 4417
Fragments arranged by size:
4417 971 919
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4498 4532 1903
Size: 34 2561 34 3678
Fragments arranged by size:
3678 2561 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4199 5409 4199
Size: 1210 5097
BclI T'GATC_A
Cuts at: 1548 2370 2415 3193 1548
Size: 822 45 778 4662
Fragments arranged by size:
4662 822 778 45
BfrBI ATG'CAT
Cuts at: 2759 2759
Size: 6307
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4918 136
Size: 107 122 71 631 605 3246 1525
Fragments arranged by size:
3246 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6302 6302
Size: 6307
BlpI GC'TnA_GC
Cuts at: 2129 3724 2129
Size: 1595 4712
Bme1580I G_kGCm'C
Cuts at: 1790 2109 2558 4368 5524 1790
Size: 319 449 1810 1156 2573
Fragments arranged by size:
2573 1810 1156 449 319
BmgBI CAC'GTC
Cuts at: 1318 3478 1318
Size: 2160 4147
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4038 4996 442
Size: 416 53 83 399 24 2621 958 1753
Fragments arranged by size:
2621 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6307
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2003 2213 2919 3255 3322 4967 1239
Size: 764 210 706 336 67 1645 2579
Fragments arranged by size:
2579 1645 764 706 336 210 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2542 4432 5297 5595 2542
Size: 1890 865 298 3254
Fragments arranged by size:
3254 1890 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2944 4970 882
Size: 617 1445 2026 2219
Fragments arranged by size:
2219 2026 1445 617
BsaAI yAC'GTr
Cuts at: 493 1204 4098 493
Size: 711 2894 2702
Fragments arranged by size:
2894 2702 711
BsaBI GATnn'nnATC
Cuts at: 6301 6301
Size: 6307
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4114 4496 5131 275
Size: 53 83 186 936 2581 382 635 1451
Fragments arranged by size:
2581 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3549 4740 5138 5412 5559 5970 3549
Size: 1191 398 274 147 411 3886
Fragments arranged by size:
3886 1191 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2561 2591 3222 3252 1178
Size: 30 165 30 1158 30 631 30 4233
Fragments arranged by size:
4233 1158 631 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2051 1186
Size: 865 5442
BseYI C'CCAG_C
Cuts at: 2284 5510 6006 2284
Size: 3226 496 2585
Fragments arranged by size:
3226 2585 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5734
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3757 4518 5546 1540
Size: 275 186 1756 761 1028 2301
Fragments arranged by size:
2301 1756 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4368 4453 5524 4368
Size: 85 1071 5151
Fragments arranged by size:
5151 1071 85
BsmI GAATG_Cn'
Cuts at: 3818 3911 3818
Size: 93 6214
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2109 2558 3626 4368 4453 5524 1391
Size: 399 319 449 1068 742 85 1071 2174
Fragments arranged by size:
2174 1071 1068 742 449 399 319 85
BspEI T'CCGG_A
Cuts at: 5970 5970
Size: 6307
BspHI T'CATG_A
Cuts at: 4196 5926 4196
Size: 1730 4577
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2433 3435 3662 844
Size: 1589 1002 227 3489
Fragments arranged by size:
3489 1589 1002 227
BsrBI CCG'CTC
Cuts at: 1394 1823 4194 1394
Size: 429 2371 3507
Fragments arranged by size:
3507 2371 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3163 4802 4976 65
Size: 1183 1915 1639 174 1396
Fragments arranged by size:
1915 1639 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4951 5138 1700
Size: 110 3141 187 2869
Fragments arranged by size:
3141 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2205 96
Size: 2109 4198
BssHII G'CGCG_C
Cuts at: 1925 3417 1925
Size: 1492 4815
BssSI C'ACGA_G
Cuts at: 4367 5379 4367
Size: 1012 5295
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2705 794
Size: 1911 4396
BstBI TT'CG_AA
Cuts at: 5111 5111
Size: 6307
BstEII G'GTnAC_C
Cuts at: 1184 3092 1184
Size: 1908 4399
BstYI r'GATC_y
Cuts at: 1972 2743 3637 4004 4389 4406 5858 5944 5956
Size: 771 894 367 385 17 1452 86 12
Cuts at: 5956 6302 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 894 771 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3133 3168 513
Size: 552 939 1129 35 3652
Fragments arranged by size:
3652 1129 939 552 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5568
Fragments arranged by size:
5568 683 56
BtsI GCAGTG_nn'
Cuts at: 3823 4617 4637 3823
Size: 794 20 5493
Fragments arranged by size:
5493 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6307
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6272
DraI TTT'AAA
Cuts at: 3529 3611 3529
Size: 82 6225
DraIII CAC_nnn'GTG
Cuts at: 2176 2562 2176
Size: 386 5921
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5314 809
Size: 507 3998 1802
Fragments arranged by size:
3998 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2040 3378
Size: 54 1425 50 275 18 177 33 1338
Cuts at: 3378 3495 3754 4643 6272 8
Size: 117 259 889 1629 43
Fragments arranged by size:
1629 1425 1338 889 275 259 177 117
54 50 43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 3754 1537
Size: 275 1942 4090
Fragments arranged by size:
4090 1942 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2211 4237 1353
Size: 858 2026 3423
Fragments arranged by size:
3423 2026 858
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4894 5268 5414 387
Size: 1064 138 3305 374 146 1280
Fragments arranged by size:
3305 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2003 2213 2919 3255 3288 3322 4370
Size: 393 371 210 706 336 33 34 1048
Cuts at: 4370 4967 5754 6022 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1048 787 706 597 393 371 336
268 210 34 33
EcoNI CCTnn'n_nnAGG
Cuts at: 3347 3347
Size: 6307
EcoO109I rG'GnC_Cy
Cuts at: 2152 2410 2152
Size: 258 6049
EcoRI G'AATT_C
Cuts at: 3616 3994 3616
Size: 378 5929
FspI TGC'GCA
Cuts at: 4813 4813
Size: 6307
HaeII r_GCGC'y
Cuts at: 1678 5454 1678
Size: 3776 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2922 2954 4948 4980 5022
Size: 32 135 32 1350 32 1994 32 42
Cuts at: 5022 5054 1373
Size: 32 2626
Fragments arranged by size:
2626 1994 1350 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3651 3897 669
Size: 988 281 1713 246 3079
Fragments arranged by size:
3079 1713 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6307
HpaI GTT'AAC
Cuts at: 3897 3897
Size: 6307
KpnI G_GTAC'C
Cuts at: 3460 3632 3460
Size: 172 6135
MfeI C'AATT_G
Cuts at: 2970 3906 2970
Size: 936 5371
MluI A'CGCG_T
Cuts at: 6183 6183
Size: 6307
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5421 5605 1305
Size: 4116 184 2007
Fragments arranged by size:
4116 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2042 10
Size: 54 1425 343 177 33 4275
Fragments arranged by size:
4275 1425 343 177 54 33
MslI CAynn'nnrTG
Cuts at: 518 1166 2427 2764 3173 4265 4624 4783 518
Size: 648 1261 337 409 1092 359 159 2042
Fragments arranged by size:
2042 1261 1092 648 409 359 337 159
MspA1I CmG'CkG
Cuts at: 1606 2038 3370 3723 4400 5548 5793 1606
Size: 432 1332 353 677 1148 245 2120
Fragments arranged by size:
2120 1332 1148 677 432 353 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3133 3168 513
Size: 552 939 1129 35 3652
Fragments arranged by size:
3652 1129 939 552 35
NdeI CA'TA_TG
Cuts at: 387 3152 387
Size: 2765 3542
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6307
NotI GC'GGCC_GC
Cuts at: 3754 3754
Size: 6307
NsiI A_TGCA'T
Cuts at: 2761 2761
Size: 6307
NspI r_CATG'y
Cuts at: 1132 1229 2137 2432 2647 3665 5210 1132
Size: 97 908 295 215 1018 1545 2229
Fragments arranged by size:
2229 1545 1018 908 295 215 97
PasI CC'CwG_GG
Cuts at: 1348 2076 1348
Size: 728 5579
PciI A'CATG_T
Cuts at: 5206 5206
Size: 6307
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2176 2179 2253 3472 1108
Size: 1068 3 74 1219 3943
Fragments arranged by size:
3943 1219 1068 74 3
PmeI GTTT'AAAC
Cuts at: 3611 3611
Size: 6307
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4376 4408 5889 5921 4376
Size: 32 1481 32 4762
Fragments arranged by size:
4762 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2152 2152
Size: 6307
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6307
PsiI TTA'TAA
Cuts at: 3877 3877
Size: 6307
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2633 2665 2633
Size: 32 6275
PstI C_TGCA'G
Cuts at: 830 3659 830
Size: 2829 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6307
PvuII CAG'CTG
Cuts at: 2038 2038
Size: 6307
SalI G'TCGA_C
Cuts at: 1936 3649 1936
Size: 1713 4594
SbfI CC_TGCA'GG
Cuts at: 3659 3659
Size: 6307
ScaI AGT'ACT
Cuts at: 4555 6267 4555
Size: 1712 4595
SexAI A'CCwGG_T
Cuts at: 2172 3452 2172
Size: 1280 5027
SfcI C'TryA_G
Cuts at: 826 1045 3655 4790 5471 5662 826
Size: 219 2610 1135 681 191 1471
Fragments arranged by size:
2610 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6307
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6307
SmaI CCC'GGG
Cuts at: 1872 3634 1872
Size: 1762 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2521 4411 5312 5574 820
Size: 197 926 578 1890 901 262 1553
Fragments arranged by size:
1890 1553 926 901 578 262 197
SnaBI TAC'GTA
Cuts at: 493 4098 493
Size: 3605 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6307
SphI G_CATG'C
Cuts at: 1132 2137 2432 3665 1132
Size: 1005 295 1233 3774
Fragments arranged by size:
3774 1233 1005 295
SspI AAT'ATT
Cuts at: 5 52 4231 5
Size: 47 4179 2081
Fragments arranged by size:
4179 2081 47
StuI AGG'CCT
Cuts at: 1517 2144 1517
Size: 627 5680
StyI C'CwwG_G
Cuts at: 513 1065 2004 3133 3168 3471 3746 513
Size: 552 939 1129 35 303 275 3074
Fragments arranged by size:
3074 1129 939 552 303 275 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3134 3379 4334 4351 4504 4689 5134
Size: 1255 1554 245 955 17 153 185 445
Cuts at: 5134 325
Size: 1498
Fragments arranged by size:
1554 1498 1255 955 445 245 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2205 4553 6265
Size: 274 80 33 51 1443 228 2348 1712
Cuts at: 6265 96
Size: 138
Fragments arranged by size:
2348 1712 1443 274 228 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2548 2580 3222 3254 6267 6299 2548
Size: 32 642 32 3013 32 2556
Fragments arranged by size:
3013 2556 642 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6307
XbaI T'CTAG_A
Cuts at: 2336 3643 2336
Size: 1307 5000
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6307
XmaI C'CCGG_G
Cuts at: 1870 3632 1870
Size: 1762 4545
XmnI GAAnn'nnTTC
Cuts at: 2225 2319 4436 2225
Size: 94 2117 4096
Fragments arranged by size:
4096 2117 94
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4115 276
Size: 53 83 186 936 2581 2468
Fragments arranged by size:
2581 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AvrII BaeI BbeI BbvCI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PspOMI PspXI RsrII SacI SacII SanDI
SapI SfiI SfoI SrfI SwaI XcmI