Restriction map for
pFN21AE6447
(Circular) (Six-base) MAPSORT of: pFN21AE6447.seq Check: 3001 from: 1 to: 6223
pFN21AE6447 6223 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4033 278
Size: 53 83 186 936 2497 2468
Fragments arranged by size:
2497 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3566 1937
Size: 1629 4594
Acc65I G'GTAC_C
Cuts at: 2189 3544 2189
Size: 1355 4868
AclI AA'CG_TT
Cuts at: 1563 2452 4350 4723 1563
Size: 889 1898 373 3063
Fragments arranged by size:
3063 1898 889 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2324 2378 4286 5670 5938 1632
Size: 692 54 1908 1384 268 1917
Fragments arranged by size:
1917 1908 1384 692 268 54
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6026
AflIII A'CryG_T
Cuts at: 1203 5122 6099 1203
Size: 3919 977 1327
Fragments arranged by size:
3919 1327 977
AgeI A'CCGG_T
Cuts at: 5054 5054
Size: 6223
AhdI GACnn_n'nnGTC
Cuts at: 4952 4952
Size: 6223
AleI CACnn'nnGTG
Cuts at: 1166 3369 1166
Size: 2203 4020
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6223
AlwNI CAG_nnn'CTG
Cuts at: 2062 2893 3966 5538 2062
Size: 831 1073 1572 2747
Fragments arranged by size:
2747 1572 1073 831
ApaLI G'TGCA_C
Cuts at: 2083 4280 5436 2083
Size: 2197 1156 2870
Fragments arranged by size:
2870 2197 1156
ApoI r'AATT_y
Cuts at: 1493 2164 2232 3484 3498 3532 3758 3910 6019
Size: 671 68 1252 14 34 226 152 2109
Cuts at: 6019 1493
Size: 1697
Fragments arranged by size:
2109 1697 1252 671 226 152 68 34
14
AseI AT'TA_AT
Cuts at: 160 1032 4777 160
Size: 872 3745 1606
Fragments arranged by size:
3745 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6223
AvaI C'yCGr_G
Cuts at: 1870 1943 2982 3548 1870
Size: 73 1039 566 4545
Fragments arranged by size:
4545 1039 566 73
BamHI G'GATC_C
Cuts at: 2863 3553 3920 2863
Size: 690 367 5166
Fragments arranged by size:
5166 690 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2189 3136 3544 4999 618
Size: 325 217 627 402 947 408 1455 1842
Fragments arranged by size:
1842 1455 947 627 408 402 325 217
BanII G_rGCy'C
Cuts at: 1391 2346 2489 3060 3542 1391
Size: 955 143 571 482 4072
Fragments arranged by size:
4072 955 571 482 143
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3206 928
Size: 971 1307 3945
Fragments arranged by size:
3945 1307 971
BbvCI CC'TCA_GC
Cuts at: 2208 2972 2208
Size: 764 5459
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2055 2089 4414 4448 1903
Size: 34 50 34 34 34 2325 34 3678
Fragments arranged by size:
3678 2325 50 34 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2848 4115 5325 2848
Size: 1267 1210 3746
Fragments arranged by size:
3746 1267 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6223
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4834 136
Size: 107 122 71 631 605 3162 1525
Fragments arranged by size:
3162 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2958 6218 2958
Size: 3260 2963
BlpI GC'TnA_GC
Cuts at: 2051 3640 2051
Size: 1589 4634
Bme1580I G_kGCm'C
Cuts at: 1790 2087 4284 5440 1790
Size: 297 2197 1156 2573
Fragments arranged by size:
2573 2197 1156 297
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6223
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3954 4912 442
Size: 416 53 83 399 24 2537 958 1753
Fragments arranged by size:
2537 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6223
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2645 2645
Size: 6223
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2915 4883 1239
Size: 1676 1968 2579
Fragments arranged by size:
2579 1968 1676
Bpu10I CC'TnA_GC
Cuts at: 2208 2972 2208
Size: 764 5459
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3219 4348 5213 5511 3219
Size: 1129 865 298 3931
Fragments arranged by size:
3931 1129 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2901 4886 882
Size: 617 1402 1985 2219
Fragments arranged by size:
2219 1985 1402 617
BsaAI yAC'GTr
Cuts at: 493 1204 4014 493
Size: 711 2810 2702
Fragments arranged by size:
2810 2702 711
BsaBI GATnn'nnATC
Cuts at: 6217 6217
Size: 6223
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4030 4412 5047 275
Size: 53 83 186 936 2497 382 635 1451
Fragments arranged by size:
2497 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4656 5054 5328 5475 5886 4656
Size: 398 274 147 411 4993
Fragments arranged by size:
4993 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5998
Fragments arranged by size:
5998 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2704 3068 3476 1186
Size: 1518 364 408 3933
Fragments arranged by size:
3933 1518 408 364
BseYI C'CCAG_C
Cuts at: 2290 3047 3357 5426 5922 2290
Size: 757 310 2069 496 2591
Fragments arranged by size:
2591 2069 757 496 310
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3347 1163
Size: 573 1611 4039
Fragments arranged by size:
4039 1611 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2069 3673 4434 5462 1540
Size: 275 186 68 1604 761 1028 2301
Fragments arranged by size:
2301 1604 1028 761 275 186 68
BsiHKAI G_wGCw'C
Cuts at: 2087 3380 4284 4369 5440 2087
Size: 1293 904 85 1071 2870
Fragments arranged by size:
2870 1293 1071 904 85
BsmI GAATG_Cn'
Cuts at: 3384 3734 3827 3384
Size: 350 93 5780
Fragments arranged by size:
5780 350 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2087 2346 2489 3060 3380 3542 4284
Size: 399 297 259 143 571 320 162 742
Cuts at: 4284 4369 5440 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 571 399 320 297 259
162 143 85
BspEI T'CCGG_A
Cuts at: 5886 5886
Size: 6223
BspHI T'CATG_A
Cuts at: 4112 5842 4112
Size: 1730 4493
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3578 844
Size: 2734 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4110 1394
Size: 429 2287 3507
Fragments arranged by size:
3507 2287 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2538 4718 4892 65
Size: 1183 1290 2180 174 1396
Fragments arranged by size:
2180 1396 1290 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2646 4867 5054 1700
Size: 110 836 2221 187 2869
Fragments arranged by size:
2869 2221 836 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6223
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6223
BssSI C'ACGA_G
Cuts at: 4283 5295 4283
Size: 1012 5211
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6223
BstBI TT'CG_AA
Cuts at: 5027 5027
Size: 6223
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6223
BstXI CCAn_nnnn'nTGG
Cuts at: 2828 2828
Size: 6223
BstYI r'GATC_y
Cuts at: 1972 2197 2863 2958 3438 3553 3920 4305 4322
Size: 225 666 95 480 115 367 385 17
Cuts at: 4322 5774 5860 5872 6218 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 666 480 385 367 346 225
115 95 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2790 2790
Size: 6223
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5671
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5484
Fragments arranged by size:
5484 683 56
BtsI GCAGTG_nn'
Cuts at: 2130 3027 3079 3739 4533 4553 2130
Size: 897 52 660 794 20 3800
Fragments arranged by size:
3800 897 794 660 52 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6223
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6188
DraI TTT'AAA
Cuts at: 2260 3527 2260
Size: 1267 4956
DraIII CAC_nnn'GTG
Cuts at: 3136 3136
Size: 6223
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5230 809
Size: 507 3914 1802
Fragments arranged by size:
3914 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2424 3246 3450
Size: 54 1425 50 275 18 594 822 204
Cuts at: 3450 3468 3670 4559 6188 8
Size: 18 202 889 1629 43
Fragments arranged by size:
1629 1425 889 822 594 275 204 202
54 50 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 3670 1537
Size: 275 1858 4090
Fragments arranged by size:
4090 1858 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4153 1353
Size: 2800 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2665 4810 5184 5330 387
Size: 1064 138 1076 2145 374 146 1280
Fragments arranged by size:
2145 1280 1076 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2324 2378 2915 4286 4883 5670 5938
Size: 393 692 54 537 1371 597 787 268
Cuts at: 5938 1239
Size: 1524
Fragments arranged by size:
1524 1371 787 692 597 537 393 268
54
EcoO109I rG'GnC_Cy
Cuts at: 2034 2890 2899 2986 2034
Size: 856 9 87 5271
Fragments arranged by size:
5271 856 87 9
EcoRI G'AATT_C
Cuts at: 3532 3910 3532
Size: 378 5845
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2980 2980
Size: 6223
FspI TGC'GCA
Cuts at: 4729 4729
Size: 6223
HaeII r_GCGC'y
Cuts at: 1678 3094 5370 1678
Size: 1416 2276 2531
Fragments arranged by size:
2531 2276 1416
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2366 2398 2945 2977 3025
Size: 32 135 32 794 32 547 32 48
Cuts at: 3025 3057 4864 4896 4938 4970 1373
Size: 32 1807 32 42 32 2626
Fragments arranged by size:
2626 1807 794 547 135 48 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2130 3567 3813 669
Size: 988 281 192 1437 246 3079
Fragments arranged by size:
3079 1437 988 281 246 192
HindIII A'AGCT_T
Cuts at: 748 3284 748
Size: 2536 3687
HpaI GTT'AAC
Cuts at: 2130 3813 2130
Size: 1683 4540
KpnI G_GTAC'C
Cuts at: 2193 3548 2193
Size: 1355 4868
MfeI C'AATT_G
Cuts at: 3822 3822
Size: 6223
MluI A'CGCG_T
Cuts at: 6099 6099
Size: 6223
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2443 5337 5521 1305
Size: 1138 2894 184 2007
Fragments arranged by size:
2894 2007 1138 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2426 3248 3452 3470 10
Size: 54 1425 343 594 822 204 18 2763
Fragments arranged by size:
2763 1425 822 594 343 204 54 18
MslI CAynn'nnrTG
Cuts at: 518 1166 3369 4181 4540 4699 518
Size: 648 2203 812 359 159 2042
Fragments arranged by size:
2203 2042 812 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2253 3361 3639 4316 5464 5709 1606
Size: 647 1108 278 677 1148 245 2120
Fragments arranged by size:
2120 1148 1108 677 647 278 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5671
NdeI CA'TA_TG
Cuts at: 387 2214 387
Size: 1827 4396
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6223
NotI GC'GGCC_GC
Cuts at: 3670 3670
Size: 6223
NspI r_CATG'y
Cuts at: 1132 1229 3115 3581 5126 1132
Size: 97 1886 466 1545 2229
Fragments arranged by size:
2229 1886 1545 466 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6223
PciI A'CATG_T
Cuts at: 5122 5122
Size: 6223
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6223
PmeI GTTT'AAAC
Cuts at: 3527 3527
Size: 6223
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4292 4324 5805 5837 4292
Size: 32 1481 32 4678
Fragments arranged by size:
4678 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6223
PsiI TTA'TAA
Cuts at: 3793 3793
Size: 6223
PstI C_TGCA'G
Cuts at: 830 3033 3575 830
Size: 2203 542 3478
Fragments arranged by size:
3478 2203 542
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6223
PvuII CAG'CTG
Cuts at: 2253 3361 2253
Size: 1108 5115
RsrII CG'GwC_CG
Cuts at: 2095 2095
Size: 6223
SalI G'TCGA_C
Cuts at: 1936 3565 1936
Size: 1629 4594
SbfI CC_TGCA'GG
Cuts at: 3575 3575
Size: 6223
ScaI AGT'ACT
Cuts at: 4471 6183 4471
Size: 1712 4511
SfcI C'TryA_G
Cuts at: 826 1045 3029 3225 3571 4706 5387 5578 826
Size: 219 1984 196 346 1135 681 191 1471
Fragments arranged by size:
1984 1471 1135 681 346 219 196 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6223
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6223
SmaI CCC'GGG
Cuts at: 1872 3550 1872
Size: 1678 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3234 4327 5228 5490 820
Size: 197 926 1291 1093 901 262 1553
Fragments arranged by size:
1553 1291 1093 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4014 493
Size: 3521 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6223
SphI G_CATG'C
Cuts at: 1132 3581 1132
Size: 2449 3774
SspI AAT'ATT
Cuts at: 5 52 4147 5
Size: 47 4095 2081
Fragments arranged by size:
4095 2081 47
StuI AGG'CCT
Cuts at: 1517 2781 1517
Size: 1264 4959
StyI C'CwwG_G
Cuts at: 513 1065 2500 3123 3662 513
Size: 552 1435 623 539 3074
Fragments arranged by size:
3074 1435 623 552 539
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4250 4267 4420 4605 5050 325
Size: 1255 2670 17 153 185 445 1498
Fragments arranged by size:
2670 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4469 6181 96
Size: 274 80 33 51 1443 2492 1712 138
Fragments arranged by size:
2492 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3370 3402 6183 6215 3370
Size: 32 2781 32 3378
Fragments arranged by size:
3378 2781 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2816 1316
Size: 1500 4723
XbaI T'CTAG_A
Cuts at: 3559 3559
Size: 6223
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2828 2828
Size: 6223
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6223
XmaI C'CCGG_G
Cuts at: 1870 3548 1870
Size: 1678 4545
XmnI GAAnn'nnTTC
Cuts at: 4352 4352
Size: 6223
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4031 276
Size: 53 83 186 936 2497 2468
Fragments arranged by size:
2497 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII RsrII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI