Restriction map for
pFN21AE6444
(Circular) (Six-base) MAPSORT of: pFN21AE6444.seq Check: 5135 from: 1 to: 6235
pFN21AE6444 6235 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3019 3019
Size: 6235
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4045 278
Size: 53 83 186 936 2509 2468
Fragments arranged by size:
2509 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3578 1937
Size: 1641 4594
Acc65I G'GTAC_C
Cuts at: 3556 3556
Size: 6235
AclI AA'CG_TT
Cuts at: 1563 4362 4735 1563
Size: 2799 373 3063
Fragments arranged by size:
3063 2799 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4298 5682 5950 1632
Size: 2666 1384 268 1917
Fragments arranged by size:
2666 1917 1384 268
AfeI AGC'GCT
Cuts at: 2244 2244
Size: 6235
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6038
AflIII A'CryG_T
Cuts at: 1203 2727 5134 6111 1203
Size: 1524 2407 977 1327
Fragments arranged by size:
2407 1524 1327 977
AgeI A'CCGG_T
Cuts at: 5066 5066
Size: 6235
AhdI GACnn_n'nnGTC
Cuts at: 3040 3530 4964 3040
Size: 490 1434 4311
Fragments arranged by size:
4311 1434 490
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6235
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2372 2530 1184
Size: 1188 158 4889
Fragments arranged by size:
4889 1188 158
AlwNI CAG_nnn'CTG
Cuts at: 2497 2665 3300 3398 3978 5550 2497
Size: 168 635 98 580 1572 3182
Fragments arranged by size:
3182 1572 635 580 168 98
ApaLI G'TGCA_C
Cuts at: 4292 5448 4292
Size: 1156 5079
ApoI r'AATT_y
Cuts at: 1493 3544 3770 3922 6031 1493
Size: 2051 226 152 2109 1697
Fragments arranged by size:
2109 2051 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4789 160
Size: 872 3757 1606
Fragments arranged by size:
3757 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6235
AvaI C'yCGr_G
Cuts at: 1870 1943 2208 3323 3560 1870
Size: 73 265 1115 237 4545
Fragments arranged by size:
4545 1115 265 237 73
BamHI G'GATC_C
Cuts at: 3565 3932 3565
Size: 367 5868
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2088 2228 2443 2635 3556
Size: 325 217 627 301 140 215 192 921
Cuts at: 3556 5011 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 921 627 325 301 217 215
192 140
BanII G_rGCy'C
Cuts at: 1391 2209 2279 3324 3554 1391
Size: 818 70 1045 230 4072
Fragments arranged by size:
4072 1045 818 230 70
BbeI G_GCGC'C
Cuts at: 2232 2232
Size: 6235
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2772 3021 928
Size: 971 873 249 4142
Fragments arranged by size:
4142 971 873 249
BbvCI CC'TCA_GC
Cuts at: 2523 2742 2523
Size: 219 6016
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4426 4460 1903
Size: 34 50 34 2405 34 3678
Fragments arranged by size:
3678 2405 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4127 5337 4127
Size: 1210 5025
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6235
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4846 136
Size: 107 122 71 631 605 3174 1525
Fragments arranged by size:
3174 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6230 6230
Size: 6235
BlpI GC'TnA_GC
Cuts at: 3652 3652
Size: 6235
Bme1580I G_kGCm'C
Cuts at: 1790 2093 4296 5452 1790
Size: 303 2203 1156 2573
Fragments arranged by size:
2573 2203 1156 303
BmgBI CAC'GTC
Cuts at: 1318 2119 1318
Size: 801 5434
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2868 3966 4924
Size: 416 53 83 399 24 1451 1098 958
Cuts at: 4924 442
Size: 1753
Fragments arranged by size:
1753 1451 1098 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6235
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2822 2986 2822
Size: 164 6071
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2126 2681 3375 4895 1239
Size: 887 555 694 1520 2579
Fragments arranged by size:
2579 1520 887 694 555
Bpu10I CC'TnA_GC
Cuts at: 2523 2742 2523
Size: 219 6016
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2792 2956 3269 4360 5225 5523 2792
Size: 164 313 1091 865 298 3504
Fragments arranged by size:
3504 1091 865 313 298 164
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2102 4898 882
Size: 617 603 2796 2219
Fragments arranged by size:
2796 2219 617 603
BsaAI yAC'GTr
Cuts at: 493 1204 2728 4026 493
Size: 711 1524 1298 2702
Fragments arranged by size:
2702 1524 1298 711
BsaBI GATnn'nnATC
Cuts at: 6229 6229
Size: 6235
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2229 4042 4424 5059
Size: 53 83 186 936 696 1813 382 635
Cuts at: 5059 275
Size: 1451
Fragments arranged by size:
1813 1451 936 696 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3198 4668 5066 5340 5487 5898 3198
Size: 1470 398 274 147 411 3535
Fragments arranged by size:
3535 1470 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6010
Fragments arranged by size:
6010 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2959 3179 3182 3257 3425 3428 1186
Size: 1773 220 3 75 168 3 3993
Fragments arranged by size:
3993 1773 220 168 75 3 3
BseYI C'CCAG_C
Cuts at: 2134 2533 2540 3141 5438 5934 2134
Size: 399 7 601 2297 496 2435
Fragments arranged by size:
2435 2297 601 496 399 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2234 2378 2500 1163
Size: 573 498 144 122 4898
Fragments arranged by size:
4898 573 498 144 122
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3685 4446 5474 1540
Size: 275 186 1684 761 1028 2301
Fragments arranged by size:
2301 1684 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2343 2931 3114 4296 4381 5452 2343
Size: 588 183 1182 85 1071 3126
Fragments arranged by size:
3126 1182 1071 588 183 85
BsmI GAATG_Cn'
Cuts at: 3208 3746 3839 3208
Size: 538 93 5604
Fragments arranged by size:
5604 538 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2093 2209 2279 2343 2931 3114 3324
Size: 399 303 116 70 64 588 183 210
Cuts at: 3324 3554 4296 4381 5452 1391
Size: 230 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 588 399 303 230 210
183 116 85 70 64
BspEI T'CCGG_A
Cuts at: 3198 5898 3198
Size: 2700 3535
BspHI T'CATG_A
Cuts at: 4124 5854 4124
Size: 1730 4505
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3019 3590 844
Size: 2175 571 3489
Fragments arranged by size:
3489 2175 571
BsrBI CCG'CTC
Cuts at: 1394 1823 2604 4122 1394
Size: 429 781 1518 3507
Fragments arranged by size:
3507 1518 781 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2965 3505 4730 4904 65
Size: 1183 1717 540 1225 174 1396
Fragments arranged by size:
1717 1396 1225 1183 540 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2085 2405 4879 5066 1700
Size: 110 275 320 2474 187 2869
Fragments arranged by size:
2869 2474 320 275 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6235
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6235
BssSI C'ACGA_G
Cuts at: 4295 5307 4295
Size: 1012 5223
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6235
BstBI TT'CG_AA
Cuts at: 5039 5039
Size: 6235
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6235
BstYI r'GATC_y
Cuts at: 1972 3565 3932 4317 4334 5786 5872 5884 6230
Size: 1593 367 385 17 1452 86 12 346
Cuts at: 6230 1972
Size: 1977
Fragments arranged by size:
1977 1593 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2040 2040
Size: 6235
BtgI C'CryG_G
Cuts at: 513 1065 2094 3351 3492 513
Size: 552 1029 1257 141 3256
Fragments arranged by size:
3256 1257 1029 552 141
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5496
Fragments arranged by size:
5496 683 56
BtsI GCAGTG_nn'
Cuts at: 2361 3751 4545 4565 2361
Size: 1390 794 20 4031
Fragments arranged by size:
4031 1390 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6235
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2759 2794 529
Size: 35 2195 35 3970
Fragments arranged by size:
3970 2195 35 35
DraI TTT'AAA
Cuts at: 3539 3539
Size: 6235
DraIII CAC_nnn'GTG
Cuts at: 2635 2635
Size: 6235
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2561 5242 809
Size: 507 1245 2681 1802
Fragments arranged by size:
2681 1802 1245 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3682 4571 6200
Size: 54 1425 50 275 18 1852 889 1629
Cuts at: 6200 8
Size: 43
Fragments arranged by size:
1852 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3682 1537
Size: 275 1870 4090
Fragments arranged by size:
4090 1870 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2478 3312 4165 1353
Size: 1125 834 853 3423
Fragments arranged by size:
3423 1125 853 834
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2378 4822 5196 5342 387
Size: 1064 138 789 2444 374 146 1280
Fragments arranged by size:
2444 1280 1064 789 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2126 2681 3375 4298 4895 5682 5950
Size: 393 494 555 694 923 597 787 268
Cuts at: 5950 1239
Size: 1524
Fragments arranged by size:
1524 923 787 694 597 555 494 393
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2696 2741 2752 2696
Size: 45 11 6179
Fragments arranged by size:
6179 45 11
EcoO109I rG'GnC_Cy
Cuts at: 2036 2494 2758 3474 2036
Size: 458 264 716 4797
Fragments arranged by size:
4797 716 458 264
EcoRI G'AATT_C
Cuts at: 3544 3922 3544
Size: 378 5857
FspI TGC'GCA
Cuts at: 2348 4741 2348
Size: 2393 3842
HaeII r_GCGC'y
Cuts at: 1678 2232 2246 5382 1678
Size: 554 14 3136 2531
Fragments arranged by size:
3136 2531 554 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3026 3058 3516 3548 4876
Size: 32 135 32 1454 32 458 32 1328
Cuts at: 4876 4908 4950 4982 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1454 1328 458 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3579 3825 669
Size: 988 281 1641 246 3079
Fragments arranged by size:
3079 1641 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6235
HpaI GTT'AAC
Cuts at: 3825 3825
Size: 6235
KasI G'GCGC_C
Cuts at: 2228 2228
Size: 6235
KpnI G_GTAC'C
Cuts at: 3560 3560
Size: 6235
MfeI C'AATT_G
Cuts at: 3834 3834
Size: 6235
MluI A'CGCG_T
Cuts at: 6111 6111
Size: 6235
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5349 5533 1305
Size: 4044 184 2007
Fragments arranged by size:
4044 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4413
Fragments arranged by size:
4413 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4193 4552 4711 518
Size: 648 3027 359 159 2042
Fragments arranged by size:
3027 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2096 2522 2934 3651 4328 5476 5721 1606
Size: 490 426 412 717 677 1148 245 2120
Fragments arranged by size:
2120 1148 717 677 490 426 412 245
NaeI GCC'GGC
Cuts at: 2407 2407
Size: 6235
NarI GG'CG_CC
Cuts at: 2229 2229
Size: 6235
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5683
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6235
NgoMIV G'CCGG_C
Cuts at: 2405 2405
Size: 6235
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6235
NotI GC'GGCC_GC
Cuts at: 3682 3682
Size: 6235
NspI r_CATG'y
Cuts at: 1132 1229 3593 5138 1132
Size: 97 2364 1545 2229
Fragments arranged by size:
2364 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2017 1348
Size: 669 5566
PciI A'CATG_T
Cuts at: 5134 5134
Size: 6235
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3398 1108
Size: 2290 3945
PfoI T'CCnGG_A
Cuts at: 2697 2697
Size: 6235
PmeI GTTT'AAAC
Cuts at: 3539 3539
Size: 6235
PmlI CAC'GTG
Cuts at: 2728 2728
Size: 6235
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4304 4336 5817 5849 4304
Size: 32 1481 32 4690
Fragments arranged by size:
4690 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3474 3474
Size: 6235
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6235
PsiI TTA'TAA
Cuts at: 3805 3805
Size: 6235
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2229 2261 2229
Size: 32 6203
PstI C_TGCA'G
Cuts at: 830 3587 830
Size: 2757 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6235
PvuII CAG'CTG
Cuts at: 2522 2522
Size: 6235
SacII CC_GC'GG
Cuts at: 2097 2097
Size: 6235
SalI G'TCGA_C
Cuts at: 1936 3577 1936
Size: 1641 4594
SanDI GG'GwC_CC
Cuts at: 3474 3474
Size: 6235
SapI GCTCTTCn'nnn_
Cuts at: 3312 3312
Size: 6235
SbfI CC_TGCA'GG
Cuts at: 3587 3587
Size: 6235
ScaI AGT'ACT
Cuts at: 4483 6195 4483
Size: 1712 4523
SexAI A'CCwGG_T
Cuts at: 3528 3528
Size: 6235
SfcI C'TryA_G
Cuts at: 826 1045 3583 4718 5399 5590 826
Size: 219 2538 1135 681 191 1471
Fragments arranged by size:
2538 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2230 2230
Size: 6235
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6235
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6235
SmaI CCC'GGG
Cuts at: 1872 3562 1872
Size: 1690 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2807 2971 3284 4339 5240 5502
Size: 197 926 864 164 313 1055 901 262
Cuts at: 5502 820
Size: 1553
Fragments arranged by size:
1553 1055 926 901 864 313 262 197
164
SnaBI TAC'GTA
Cuts at: 493 4026 493
Size: 3533 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6235
SphI G_CATG'C
Cuts at: 1132 3593 1132
Size: 2461 3774
SspI AAT'ATT
Cuts at: 5 52 4159 5
Size: 47 4107 2081
Fragments arranged by size:
4107 2081 47
StuI AGG'CCT
Cuts at: 1517 2045 2747 1517
Size: 528 702 5005
Fragments arranged by size:
5005 702 528
StyI C'CwwG_G
Cuts at: 513 1065 3674 513
Size: 552 2609 3074
Fragments arranged by size:
3074 2609 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2729 3053 4262 4279 4432 4617 5062
Size: 1255 1149 324 1209 17 153 185 445
Cuts at: 5062 325
Size: 1498
Fragments arranged by size:
1498 1255 1209 1149 445 324 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4481 6193 96
Size: 274 80 33 51 1443 2504 1712 138
Fragments arranged by size:
2504 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6195 6227 6195
Size: 32 6203
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6235
XbaI T'CTAG_A
Cuts at: 3571 3571
Size: 6235
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3017 3017
Size: 6235
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6235
XmaI C'CCGG_G
Cuts at: 1870 3560 1870
Size: 1690 4545
XmnI GAAnn'nnTTC
Cuts at: 4364 4364
Size: 6235
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4043 276
Size: 53 83 186 936 2509 2468
Fragments arranged by size:
2509 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI PvuII SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI NruI
NsiI PacI PspOMI PspXI RsrII SacI SfiI SrfI
SwaI