Restriction map for
pFN21AE6443
(Circular) (Six-base) MAPSORT of: pFN21AE6443.seq Check: 1189 from: 1 to: 6052
pFN21AE6443 6052 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2963 2963
Size: 6052
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3862 278
Size: 53 83 186 936 2326 2468
Fragments arranged by size:
2468 2326 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3395 1937
Size: 1458 4594
Acc65I G'GTAC_C
Cuts at: 3373 3373
Size: 6052
AclI AA'CG_TT
Cuts at: 1563 4179 4552 1563
Size: 2616 373 3063
Fragments arranged by size:
3063 2616 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2919 3164 4115 5499 5767 1632
Size: 1287 245 951 1384 268 1917
Fragments arranged by size:
1917 1384 1287 951 268 245
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5855
AflIII A'CryG_T
Cuts at: 1203 4951 5928 1203
Size: 3748 977 1327
Fragments arranged by size:
3748 1327 977
AgeI A'CCGG_T
Cuts at: 4883 4883
Size: 6052
AhdI GACnn_n'nnGTC
Cuts at: 3078 4781 3078
Size: 1703 4349
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6052
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2971 1184
Size: 1787 4265
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2745 2777 2745
Size: 32 6020
AlwNI CAG_nnn'CTG
Cuts at: 2942 3795 5367 2942
Size: 853 1572 3627
Fragments arranged by size:
3627 1572 853
ApaI G_GGCC'C
Cuts at: 2040 2856 2040
Size: 816 5236
ApaLI G'TGCA_C
Cuts at: 4109 5265 4109
Size: 1156 4896
ApoI r'AATT_y
Cuts at: 1493 3361 3587 3739 5848 1493
Size: 1868 226 152 2109 1697
Fragments arranged by size:
2109 1868 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4606 160
Size: 872 3574 1606
Fragments arranged by size:
3574 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6052
AvaI C'yCGr_G
Cuts at: 1870 1943 2213 2458 2531 2548 2725 3377 1870
Size: 73 270 245 73 17 177 652 4545
Fragments arranged by size:
4545 652 270 245 177 73 73 17
BamHI G'GATC_C
Cuts at: 3382 3749 3382
Size: 367 5685
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2981 3134 3373 4828 618
Size: 325 217 627 1194 153 239 1455 1842
Fragments arranged by size:
1842 1455 1194 627 325 239 217 153
BanII G_rGCy'C
Cuts at: 1391 2040 2732 2856 3371 1391
Size: 649 692 124 515 4072
Fragments arranged by size:
4072 692 649 515 124
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2239 2287 928
Size: 971 340 48 4693
Fragments arranged by size:
4693 971 340 48
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4243 4277 1903
Size: 34 50 34 2222 34 3678
Fragments arranged by size:
3678 2222 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3944 5154 3944
Size: 1210 4842
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6052
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2527 2979 4663
Size: 107 122 71 631 605 855 452 1684
Cuts at: 4663 136
Size: 1525
Fragments arranged by size:
1684 1525 855 631 605 452 122 107
71
BglII A'GATC_T
Cuts at: 2776 6047 2776
Size: 3271 2781
BlpI GC'TnA_GC
Cuts at: 3469 3469
Size: 6052
Bme1580I G_kGCm'C
Cuts at: 1790 2040 2414 2465 2834 2856 3173 3317 4113
Size: 250 374 51 369 22 317 144 796
Cuts at: 4113 5269 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 796 374 369 317 250 144
51 22
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6052
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3783 4741 442
Size: 416 53 83 399 24 2366 958 1753
Fragments arranged by size:
2366 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6052
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2276 2309 2513 2645 3123 3228 4712 1239
Size: 1037 33 204 132 478 105 1484 2579
Fragments arranged by size:
2579 1484 1037 478 204 132 105 33
Bpu10I CC'TnA_GC
Cuts at: 3299 3299
Size: 6052
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4177 5042 5340 4177
Size: 865 298 4889
Fragments arranged by size:
4889 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4715 882
Size: 617 3216 2219
Fragments arranged by size:
3216 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2146 3154 3843 493
Size: 711 942 1008 689 2702
Fragments arranged by size:
2702 1008 942 711 689
BsaBI GATnn'nnATC
Cuts at: 6046 6046
Size: 6052
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3859 4241 4876 275
Size: 53 83 186 936 2326 382 635 1451
Fragments arranged by size:
2326 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2911 4485 4883 5157 5304 5715 2911
Size: 1574 398 274 147 411 3248
Fragments arranged by size:
3248 1574 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2109 2139 1178
Size: 30 165 30 706 30 5091
Fragments arranged by size:
5091 706 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 2728 2747 1186
Size: 847 695 19 4491
Fragments arranged by size:
4491 847 695 19
BseYI C'CCAG_C
Cuts at: 2300 2698 5255 5751 2300
Size: 398 2557 496 2601
Fragments arranged by size:
2601 2557 496 398
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2246 1163
Size: 573 510 4969
Fragments arranged by size:
4969 573 510
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3502 4263 5291 1540
Size: 275 186 1501 761 1028 2301
Fragments arranged by size:
2301 1501 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2472 4113 4198 5269 2472
Size: 1641 85 1071 3255
Fragments arranged by size:
3255 1641 1071 85
BsmI GAATG_Cn'
Cuts at: 3563 3656 3563
Size: 93 5959
BsmBI CGTCTCn'nnnn_
Cuts at: 2776 2776
Size: 6052
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2414 2465 2472 2732 2834 2856
Size: 399 250 374 51 7 260 102 22
Cuts at: 2856 3173 3317 3371 4113 4198 5269 1391
Size: 317 144 54 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 374 317 260 250
144 102 85 54 51 22 7
BspEI T'CCGG_A
Cuts at: 2911 5715 2911
Size: 2804 3248
BspHI T'CATG_A
Cuts at: 3941 5671 3941
Size: 1730 4322
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2963 3123 3407 844
Size: 2119 160 284 3489
Fragments arranged by size:
3489 2119 284 160
BsrBI CCG'CTC
Cuts at: 1394 1823 3939 1394
Size: 429 2116 3507
Fragments arranged by size:
3507 2116 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4547 4721 65
Size: 1183 3299 174 1396
Fragments arranged by size:
3299 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3267 4696 4883 1700
Size: 110 1457 1429 187 2869
Fragments arranged by size:
2869 1457 1429 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6052
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6052
BssSI C'ACGA_G
Cuts at: 4112 5124 4112
Size: 1012 5040
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3168 794
Size: 2374 3678
BstBI TT'CG_AA
Cuts at: 4856 4856
Size: 6052
BstEII G'GTnAC_C
Cuts at: 1184 2383 1184
Size: 1199 4853
BstYI r'GATC_y
Cuts at: 1972 2776 3285 3382 3749 4134 4151 5603 5689
Size: 804 509 97 367 385 17 1452 86
Cuts at: 5689 5701 6047 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 804 509 385 367 346 97
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2390 2390
Size: 6052
BtgI C'CryG_G
Cuts at: 513 1065 2825 3039 513
Size: 552 1760 214 3526
Fragments arranged by size:
3526 1760 552 214
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2560 2581 487
Size: 683 56 1334 21 3958
Fragments arranged by size:
3958 1334 683 56 21
BtsI GCAGTG_nn'
Cuts at: 2046 2832 2972 3173 3568 4362 4382 2046
Size: 786 140 201 395 794 20 3716
Fragments arranged by size:
3716 794 786 395 201 140 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6052
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6017
DraI TTT'AAA
Cuts at: 3356 3356
Size: 6052
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3034 5059 809
Size: 507 1718 2025 1802
Fragments arranged by size:
2025 1802 1718 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2317 3499 4388
Size: 54 1425 50 275 18 487 1182 889
Cuts at: 4388 6017 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1182 889 487 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3499 1537
Size: 275 1687 4090
Fragments arranged by size:
4090 1687 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3213 3982 1353
Size: 1860 769 3423
Fragments arranged by size:
3423 1860 769
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4639 5013 5159 387
Size: 1064 138 3050 374 146 1280
Fragments arranged by size:
3050 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2276 2309 2513 2645 2919 3123 3164
Size: 393 644 33 204 132 274 204 41
Cuts at: 3164 3228 4115 4712 5499 5767 1239
Size: 64 887 597 787 268 1524
Fragments arranged by size:
1524 887 787 644 597 393 274 268
204 204 132 64 41 33
EcoO109I rG'GnC_Cy
Cuts at: 2036 2037 2343 2852 3061 3230 2036
Size: 1 306 509 209 169 4858
Fragments arranged by size:
4858 509 306 209 169 1
EcoRI G'AATT_C
Cuts at: 3361 3739 3361
Size: 378 5674
FspI TGC'GCA
Cuts at: 4558 4558
Size: 6052
HaeII r_GCGC'y
Cuts at: 1678 5199 1678
Size: 3521 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2786 2818 3064 3096 3219
Size: 32 135 32 1214 32 246 32 123
Cuts at: 3219 3251 4693 4725 4767 4799 1373
Size: 32 1442 32 42 32 2626
Fragments arranged by size:
2626 1442 1214 246 135 123 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3396 3642 669
Size: 988 281 1458 246 3079
Fragments arranged by size:
3079 1458 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6052
HpaI GTT'AAC
Cuts at: 3642 3642
Size: 6052
KpnI G_GTAC'C
Cuts at: 3377 3377
Size: 6052
MfeI C'AATT_G
Cuts at: 3651 3651
Size: 6052
MluI A'CGCG_T
Cuts at: 5928 5928
Size: 6052
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5166 5350 1305
Size: 3861 184 2007
Fragments arranged by size:
3861 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4230
Fragments arranged by size:
4230 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4010 4369 4528 518
Size: 648 2844 359 159 2042
Fragments arranged by size:
2844 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2323 2445 2559 2792 2827 3468 4145 5293
Size: 717 122 114 233 35 641 677 1148
Cuts at: 5293 5538 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 717 677 641 245 233 122
114 35
NcoI C'CATG_G
Cuts at: 513 1065 3039 513
Size: 552 1974 3526
Fragments arranged by size:
3526 1974 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6052
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6052
NotI GC'GGCC_GC
Cuts at: 3499 3499
Size: 6052
NspI r_CATG'y
Cuts at: 1132 1229 3410 4955 1132
Size: 97 2181 1545 2229
Fragments arranged by size:
2229 2181 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2074 2503 1348
Size: 726 429 4897
Fragments arranged by size:
4897 726 429
PciI A'CATG_T
Cuts at: 4951 4951
Size: 6052
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3131 1108
Size: 2023 4029
PfoI T'CCnGG_A
Cuts at: 2690 2690
Size: 6052
PmeI GTTT'AAAC
Cuts at: 3356 3356
Size: 6052
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4121 4153 5634 5666 4121
Size: 32 1481 32 4507
Fragments arranged by size:
4507 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2343 2343
Size: 6052
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6052
PsiI TTA'TAA
Cuts at: 3096 3622 3096
Size: 526 5526
PspOMI G'GGCC_C
Cuts at: 2036 2852 2036
Size: 816 5236
PstI C_TGCA'G
Cuts at: 830 2017 3404 830
Size: 1187 1387 3478
Fragments arranged by size:
3478 1387 1187
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6052
PvuII CAG'CTG
Cuts at: 2559 2559
Size: 6052
SacII CC_GC'GG
Cuts at: 2828 2828
Size: 6052
SalI G'TCGA_C
Cuts at: 1936 3394 1936
Size: 1458 4594
SbfI CC_TGCA'GG
Cuts at: 3404 3404
Size: 6052
ScaI AGT'ACT
Cuts at: 4300 6012 4300
Size: 1712 4340
SexAI A'CCwGG_T
Cuts at: 2379 2589 2379
Size: 210 5842
SfcI C'TryA_G
Cuts at: 826 1045 2013 3400 4535 5216 5407 826
Size: 219 968 1387 1135 681 191 1471
Fragments arranged by size:
1471 1387 1135 968 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6052
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6052
SmaI CCC'GGG
Cuts at: 1872 2550 2727 3379 1872
Size: 678 177 652 4545
Fragments arranged by size:
4545 678 652 177
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4156 5057 5319 820
Size: 197 926 2213 901 262 1553
Fragments arranged by size:
2213 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3843 493
Size: 3350 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6052
SphI G_CATG'C
Cuts at: 1132 3410 1132
Size: 2278 3774
SspI AAT'ATT
Cuts at: 5 52 3976 5
Size: 47 3924 2081
Fragments arranged by size:
3924 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6052
StyI C'CwwG_G
Cuts at: 513 1065 3039 3491 513
Size: 552 1974 452 3074
Fragments arranged by size:
3074 1974 552 452
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2644 2965 3142 4079 4096 4249 4434
Size: 1255 1064 321 177 937 17 153 185
Cuts at: 4434 4879 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1064 937 445 321 185 177
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4298 6010 96
Size: 274 80 33 51 1443 2321 1712 138
Fragments arranged by size:
2321 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2109 2141 6012 6044 2109
Size: 32 3871 32 2117
Fragments arranged by size:
3871 2117 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2227 2591 2654 2720 1316
Size: 911 364 63 66 4648
Fragments arranged by size:
4648 911 364 66 63
XbaI T'CTAG_A
Cuts at: 3388 3388
Size: 6052
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3013 3013
Size: 6052
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6052
XmaI C'CCGG_G
Cuts at: 1870 2548 2725 3377 1870
Size: 678 177 652 4545
Fragments arranged by size:
4545 678 652 177
XmnI GAAnn'nnTTC
Cuts at: 4181 4181
Size: 6052
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3860 276
Size: 53 83 186 936 2326 2468
Fragments arranged by size:
2468 2326 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI RsrII SacI SanDI SapI
SfiI SfoI SrfI SwaI