Restriction map for
pFN21AE6442
(Circular) (Six-base) MAPSORT of: pFN21AE6442.seq Check: 163 from: 1 to: 6070
pFN21AE6442 6070 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3880 278
Size: 53 83 186 936 2344 2468
Fragments arranged by size:
2468 2344 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2838 3413 1937
Size: 901 575 4594
Fragments arranged by size:
4594 901 575
Acc65I G'GTAC_C
Cuts at: 3391 3391
Size: 6070
AclI AA'CG_TT
Cuts at: 1563 4197 4570 1563
Size: 2634 373 3063
Fragments arranged by size:
3063 2634 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3237 4133 5517 5785 1632
Size: 1605 896 1384 268 1917
Fragments arranged by size:
1917 1605 1384 896 268
AfeI AGC'GCT
Cuts at: 2903 2903
Size: 6070
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5873
AflIII A'CryG_T
Cuts at: 1203 4969 5946 1203
Size: 3766 977 1327
Fragments arranged by size:
3766 1327 977
AgeI A'CCGG_T
Cuts at: 4901 4901
Size: 6070
AhdI GACnn_n'nnGTC
Cuts at: 4799 4799
Size: 6070
AleI CACnn'nnGTG
Cuts at: 1166 2166 1166
Size: 1000 5070
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6070
AlwNI CAG_nnn'CTG
Cuts at: 2308 3337 3813 5385 2308
Size: 1029 476 1572 2993
Fragments arranged by size:
2993 1572 1029 476
ApaI G_GGCC'C
Cuts at: 2579 3031 3240 3277 2579
Size: 452 209 37 5372
Fragments arranged by size:
5372 452 209 37
ApaLI G'TGCA_C
Cuts at: 4127 5283 4127
Size: 1156 4914
ApoI r'AATT_y
Cuts at: 1493 3379 3605 3757 5866 1493
Size: 1886 226 152 2109 1697
Fragments arranged by size:
2109 1886 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4624 160
Size: 872 3592 1606
Fragments arranged by size:
3592 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6070
AvaI C'yCGr_G
Cuts at: 1870 1943 2144 2863 3395 1870
Size: 73 201 719 532 4545
Fragments arranged by size:
4545 719 532 201 73
AvrII C'CTAG_G
Cuts at: 3211 3211
Size: 6070
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2242 2275 2242
Size: 33 6037
BamHI G'GATC_C
Cuts at: 2991 3400 3767 2991
Size: 409 367 5294
Fragments arranged by size:
5294 409 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2352 2611 2698 2975 3043
Size: 325 217 627 565 259 87 277 68
Cuts at: 3043 3133 3391 4846 618
Size: 90 258 1455 1842
Fragments arranged by size:
1842 1455 627 565 325 277 259 258
217 90 87 68
BanII G_rGCy'C
Cuts at: 1391 2510 2579 3031 3240 3277 3306 3389 1391
Size: 1119 69 452 209 37 29 83 4072
Fragments arranged by size:
4072 1119 452 209 83 69 37 29
BbeI G_GCGC'C
Cuts at: 2356 2702 2356
Size: 346 5724
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5099
BbvCI CC'TCA_GC
Cuts at: 3366 3366
Size: 6070
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4261 4295 1903
Size: 34 2324 34 3678
Fragments arranged by size:
3678 2324 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3962 5172 3962
Size: 1210 4860
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6070
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2435 2829 4681
Size: 107 122 71 631 605 763 394 1852
Cuts at: 4681 136
Size: 1525
Fragments arranged by size:
1852 1525 763 631 605 394 122 107
71
BglII A'GATC_T
Cuts at: 6065 6065
Size: 6070
BlpI GC'TnA_GC
Cuts at: 2741 2810 2882 3487 2741
Size: 69 72 605 5324
Fragments arranged by size:
5324 605 72 69
Bme1580I G_kGCm'C
Cuts at: 1790 2173 2579 3031 3048 3138 3188 3240 3277
Size: 383 406 452 17 90 50 52 37
Cuts at: 3277 4131 5287 1790
Size: 854 1156 2573
Fragments arranged by size:
2573 1156 854 452 406 383 90 52
50 37 17
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6070
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2334 2570 3801
Size: 416 53 83 399 24 917 236 1231
Cuts at: 3801 4759 442
Size: 958 1753
Fragments arranged by size:
1753 1231 958 917 416 399 236 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6070
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2825 2825
Size: 6070
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2425 2708 2820 2891 4730 1239
Size: 1186 283 112 71 1839 2579
Fragments arranged by size:
2579 1839 1186 283 112 71
Bpu10I CC'TnA_GC
Cuts at: 3366 3366
Size: 6070
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3344 4195 5060 5358 3344
Size: 851 865 298 4056
Fragments arranged by size:
4056 865 851 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2279 4733 882
Size: 617 780 2454 2219
Fragments arranged by size:
2454 2219 780 617
BsaAI yAC'GTr
Cuts at: 493 1204 3294 3861 493
Size: 711 2090 567 2702
Fragments arranged by size:
2702 2090 711 567
BsaBI GATnn'nnATC
Cuts at: 6064 6064
Size: 6070
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2353 2699 3877 4259
Size: 53 83 186 936 820 346 1178 382
Cuts at: 4259 4894 275
Size: 635 1451
Fragments arranged by size:
1451 1178 936 820 635 382 346 186
83 53
BsaWI w'CCGG_w
Cuts at: 4503 4901 5175 5322 5733 4503
Size: 398 274 147 411 4840
Fragments arranged by size:
4840 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2234 2264 1178
Size: 30 165 30 831 30 4984
Fragments arranged by size:
4984 831 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3047 1186
Size: 1861 4209
BseYI C'CCAG_C
Cuts at: 3066 3088 5273 5769 3066
Size: 22 2185 496 3367
Fragments arranged by size:
3367 2185 496 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2014 1163
Size: 573 278 5219
Fragments arranged by size:
5219 573 278
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2167 3520 4281 5309 1540
Size: 275 186 166 1353 761 1028 2301
Fragments arranged by size:
2301 1353 1028 761 275 186 166
BsiHKAI G_wGCw'C
Cuts at: 3220 4131 4216 5287 3220
Size: 911 85 1071 4003
Fragments arranged by size:
4003 1071 911 85
BsmI GAATG_Cn'
Cuts at: 2288 3581 3674 2288
Size: 1293 93 4684
Fragments arranged by size:
4684 1293 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2669 2669
Size: 6070
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2173 2510 2579 3031 3048 3138 3188
Size: 399 383 337 69 452 17 90 50
Cuts at: 3188 3220 3240 3277 3306 3389 4131 4216 5287
Size: 32 20 37 29 83 742 85 1071
Cuts at: 5287 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 452 399 383 337 90
85 83 69 50 37 32 29 20
17
BspEI T'CCGG_A
Cuts at: 5733 5733
Size: 6070
BspHI T'CATG_A
Cuts at: 3959 5689 3959
Size: 1730 4340
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2655 3425 844
Size: 1811 770 3489
Fragments arranged by size:
3489 1811 770
BsrBI CCG'CTC
Cuts at: 1394 1823 3957 1394
Size: 429 2134 3507
Fragments arranged by size:
3507 2134 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4565 4739 65
Size: 1183 3317 174 1396
Fragments arranged by size:
3317 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4714 4901 1700
Size: 110 2904 187 2869
Fragments arranged by size:
2904 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6070
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6070
BssSI C'ACGA_G
Cuts at: 2778 4130 5142 2778
Size: 1352 1012 3706
Fragments arranged by size:
3706 1352 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6070
BstBI TT'CG_AA
Cuts at: 4874 4874
Size: 6070
BstEII G'GTnAC_C
Cuts at: 1184 2077 1184
Size: 893 5177
BstYI r'GATC_y
Cuts at: 1972 2991 3400 3767 4152 4169 5621 5707 5719
Size: 1019 409 367 385 17 1452 86 12
Cuts at: 5719 6065 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1019 409 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2161 2484 2958 3150 3231 513
Size: 552 939 157 323 474 192 81 3352
Fragments arranged by size:
3352 939 552 474 323 192 157 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5331
Fragments arranged by size:
5331 683 56
BtsI GCAGTG_nn'
Cuts at: 3200 3586 4380 4400 3200
Size: 386 794 20 4870
Fragments arranged by size:
4870 794 386 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6070
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6035
DraI TTT'AAA
Cuts at: 3374 3374
Size: 6070
DraIII CAC_nnn'GTG
Cuts at: 3294 3294
Size: 6070
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5077 809
Size: 507 3761 1802
Fragments arranged by size:
3761 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2164 2385 2487
Size: 54 1425 50 275 18 334 221 102
Cuts at: 2487 2782 2961 3222 3246 3267 3517 4406 6035
Size: 295 179 261 24 21 250 889 1629
Cuts at: 6035 8
Size: 43
Fragments arranged by size:
1629 1425 889 334 295 275 261 250
221 179 102 54 50 43 24 21
18
EagI C'GGCC_G
Cuts at: 1537 1812 2164 3517 1537
Size: 275 352 1353 4090
Fragments arranged by size:
4090 1353 352 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2240 2636 2858 4000 1353
Size: 887 396 222 1142 3423
Fragments arranged by size:
3423 1142 887 396 222
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3004 4657 5031 5177 387
Size: 1064 138 1415 1653 374 146 1280
Fragments arranged by size:
1653 1415 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2425 2708 2820 2891 3237 4133 4730
Size: 393 793 283 112 71 346 896 597
Cuts at: 4730 5517 5785 1239
Size: 787 268 1524
Fragments arranged by size:
1524 896 793 787 597 393 346 283
268 112 71
EcoNI CCTnn'n_nnAGG
Cuts at: 2924 2924
Size: 6070
EcoO109I rG'GnC_Cy
Cuts at: 2576 2788 3028 3236 3237 3273 2576
Size: 212 240 208 1 36 5373
Fragments arranged by size:
5373 240 212 208 36 1
EcoRI G'AATT_C
Cuts at: 3379 3757 3379
Size: 378 5692
FspI TGC'GCA
Cuts at: 3206 4576 3206
Size: 1370 4700
HaeII r_GCGC'y
Cuts at: 1678 2071 2356 2702 2905 5217 1678
Size: 393 285 346 203 2312 2531
Fragments arranged by size:
2531 2312 393 346 285 203
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2492 2524 2780 2812 4711
Size: 32 135 32 920 32 256 32 1899
Cuts at: 4711 4743 4785 4817 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1899 920 256 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3414 3660 669
Size: 988 281 1476 246 3079
Fragments arranged by size:
3079 1476 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6070
HpaI GTT'AAC
Cuts at: 3660 3660
Size: 6070
KasI G'GCGC_C
Cuts at: 2352 2698 2352
Size: 346 5724
KpnI G_GTAC'C
Cuts at: 3395 3395
Size: 6070
MfeI C'AATT_G
Cuts at: 3669 3669
Size: 6070
MluI A'CGCG_T
Cuts at: 5946 5946
Size: 6070
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2133 2667 5184 5368 1305
Size: 828 534 2517 184 2007
Fragments arranged by size:
2517 2007 828 534 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2387 2784 2963 3224 10
Size: 54 1425 343 555 397 179 261 2856
Fragments arranged by size:
2856 1425 555 397 343 261 179 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2166 2231 2546 3191 4028 4387 4546
Size: 648 1000 65 315 645 837 359 159
Cuts at: 4546 518
Size: 2042
Fragments arranged by size:
2042 1000 837 648 645 359 315 159
65
MspA1I CmG'CkG
Cuts at: 1606 3233 3486 4163 5311 5556 1606
Size: 1627 253 677 1148 245 2120
Fragments arranged by size:
2120 1627 1148 677 253 245
NarI GG'CG_CC
Cuts at: 2353 2699 2353
Size: 346 5724
NcoI C'CATG_G
Cuts at: 513 1065 2004 2958 513
Size: 552 939 954 3625
Fragments arranged by size:
3625 954 939 552
NdeI CA'TA_TG
Cuts at: 387 2395 387
Size: 2008 4062
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6070
NotI GC'GGCC_GC
Cuts at: 3517 3517
Size: 6070
NspI r_CATG'y
Cuts at: 1132 1229 3428 4973 1132
Size: 97 2199 1545 2229
Fragments arranged by size:
2229 2199 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2572 2713 1348
Size: 1224 141 4705
Fragments arranged by size:
4705 1224 141
PciI A'CATG_T
Cuts at: 4969 4969
Size: 6070
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2324 2798 1108
Size: 902 314 474 4380
Fragments arranged by size:
4380 902 474 314
PmeI GTTT'AAAC
Cuts at: 3374 3374
Size: 6070
PmlI CAC'GTG
Cuts at: 3294 3294
Size: 6070
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4139 4171 5652 5684 4139
Size: 32 1481 32 4525
Fragments arranged by size:
4525 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2788 2788
Size: 6070
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6070
PsiI TTA'TAA
Cuts at: 3640 3640
Size: 6070
PspOMI G'GGCC_C
Cuts at: 2575 3027 3236 3273 2575
Size: 452 209 37 5372
Fragments arranged by size:
5372 452 209 37
PstI C_TGCA'G
Cuts at: 830 2304 3333 3422 830
Size: 1474 1029 89 3478
Fragments arranged by size:
3478 1474 1029 89
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6070
SacII CC_GC'GG
Cuts at: 3234 3234
Size: 6070
SalI G'TCGA_C
Cuts at: 1936 3412 1936
Size: 1476 4594
SapI GCTCTTCn'nnn_
Cuts at: 2636 2636
Size: 6070
SbfI CC_TGCA'GG
Cuts at: 3422 3422
Size: 6070
ScaI AGT'ACT
Cuts at: 4318 6030 4318
Size: 1712 4358
SfcI C'TryA_G
Cuts at: 826 1045 2300 2921 3329 3418 4553 5234 5425
Size: 219 1255 621 408 89 1135 681 191
Cuts at: 5425 826
Size: 1471
Fragments arranged by size:
1471 1255 1135 681 621 408 219 191
89
SfiI GGCCn_nnn'nGGCC
Cuts at: 2435 2435
Size: 6070
SfoI GGC'GCC
Cuts at: 2354 2700 2354
Size: 346 5724
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6070
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6070
SmaI CCC'GGG
Cuts at: 1872 2146 3397 1872
Size: 274 1251 4545
Fragments arranged by size:
4545 1251 274
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3359 4174 5075 5337 820
Size: 197 926 1416 815 901 262 1553
Fragments arranged by size:
1553 1416 926 901 815 262 197
SnaBI TAC'GTA
Cuts at: 493 3861 493
Size: 3368 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6070
SphI G_CATG'C
Cuts at: 1132 3428 1132
Size: 2296 3774
SrfI GCCC'GGGC
Cuts at: 2146 2146
Size: 6070
SspI AAT'ATT
Cuts at: 5 52 3994 5
Size: 47 3942 2081
Fragments arranged by size:
3942 2081 47
StuI AGG'CCT
Cuts at: 1517 3281 1517
Size: 1764 4306
StyI C'CwwG_G
Cuts at: 513 1065 2004 2958 3211 3509 513
Size: 552 939 954 253 298 3074
Fragments arranged by size:
3074 954 939 552 298 253
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2059 2369 4097 4114 4267 4452 4897
Size: 1255 479 310 1728 17 153 185 445
Cuts at: 4897 325
Size: 1498
Fragments arranged by size:
1728 1498 1255 479 445 310 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2874 3258 4316
Size: 274 80 33 51 1443 897 384 1058
Cuts at: 4316 6028 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1058 897 384 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6030 6062 6030
Size: 32 6038
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6070
XbaI T'CTAG_A
Cuts at: 3406 3406
Size: 6070
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6070
XmaI C'CCGG_G
Cuts at: 1870 2144 3395 1870
Size: 274 1251 4545
Fragments arranged by size:
4545 1251 274
XmnI GAAnn'nnTTC
Cuts at: 4199 4199
Size: 6070
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3878 276
Size: 53 83 186 936 2344 2468
Fragments arranged by size:
2468 2344 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BfrBI BsiWI BstXI BstZ17I Bsu36I
EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PfoI PspXI PsrI PvuII RsrII SacI
SanDI SexAI SwaI XcmI