Restriction map for
pFN21AE6441
(Circular) (Six-base) MAPSORT of: pFN21AE6441.seq Check: 2055 from: 1 to: 6097
pFN21AE6441 6097 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3907 278
Size: 53 83 186 936 2371 2468
Fragments arranged by size:
2468 2371 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2646 3440 1937
Size: 709 794 4594
Fragments arranged by size:
4594 794 709
Acc65I G'GTAC_C
Cuts at: 3418 3418
Size: 6097
AclI AA'CG_TT
Cuts at: 1563 4224 4597 1563
Size: 2661 373 3063
Fragments arranged by size:
3063 2661 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2261 2315 4160 5544 5812 1632
Size: 629 54 1845 1384 268 1917
Fragments arranged by size:
1917 1845 1384 629 268 54
AfeI AGC'GCT
Cuts at: 2078 2078
Size: 6097
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5900
AflIII A'CryG_T
Cuts at: 1203 2816 3105 3147 4996 5973 1203
Size: 1613 289 42 1849 977 1327
Fragments arranged by size:
1849 1613 1327 977 289 42
AgeI A'CCGG_T
Cuts at: 4928 4928
Size: 6097
AhdI GACnn_n'nnGTC
Cuts at: 4826 4826
Size: 6097
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6097
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6097
AlwNI CAG_nnn'CTG
Cuts at: 2753 3840 5412 2753
Size: 1087 1572 3438
Fragments arranged by size:
3438 1572 1087
ApaI G_GGCC'C
Cuts at: 2298 2743 2298
Size: 445 5652
ApaLI G'TGCA_C
Cuts at: 4154 5310 4154
Size: 1156 4941
ApoI r'AATT_y
Cuts at: 1493 2250 3406 3632 3784 5893 1493
Size: 757 1156 226 152 2109 1697
Fragments arranged by size:
2109 1697 1156 757 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4651 160
Size: 872 3619 1606
Fragments arranged by size:
3619 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6097
AvaI C'yCGr_G
Cuts at: 1870 1943 2298 3422 1870
Size: 73 355 1124 4545
Fragments arranged by size:
4545 1124 355 73
BamHI G'GATC_C
Cuts at: 3427 3794 3427
Size: 367 5730
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2082 2375 2562 3418 4873
Size: 325 217 627 295 293 187 856 1455
Cuts at: 4873 618
Size: 1842
Fragments arranged by size:
1842 1455 856 627 325 295 293 217
187
BanII G_rGCy'C
Cuts at: 1391 2235 2298 2743 3416 1391
Size: 844 63 445 673 4072
Fragments arranged by size:
4072 844 673 445 63
BbeI G_GCGC'C
Cuts at: 2086 2566 2086
Size: 480 5617
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2613 2880 928
Size: 971 714 267 4145
Fragments arranged by size:
4145 971 714 267
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4288 4322 1903
Size: 34 2351 34 3678
Fragments arranged by size:
3678 2351 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3989 5199 3989
Size: 1210 4887
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6097
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4708 136
Size: 107 122 71 631 605 337 2699 1525
Fragments arranged by size:
2699 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6092 6092
Size: 6097
BlpI GC'TnA_GC
Cuts at: 3258 3514 3258
Size: 256 5841
Bme1580I G_kGCm'C
Cuts at: 1790 2298 2743 4158 5314 1790
Size: 508 445 1415 1156 2573
Fragments arranged by size:
2573 1415 1156 508 445
BmgBI CAC'GTC
Cuts at: 1318 2131 3108 1318
Size: 813 977 4307
Fragments arranged by size:
4307 977 813
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2756 3828 4786
Size: 416 53 83 399 24 1339 1072 958
Cuts at: 4786 442
Size: 1753
Fragments arranged by size:
1753 1339 1072 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6097
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2375 3068 3097 4757 1239
Size: 1136 693 29 1660 2579
Fragments arranged by size:
2579 1660 1136 693 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4222 5087 5385 4222
Size: 865 298 4934
Fragments arranged by size:
4934 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4760 882
Size: 617 3261 2219
Fragments arranged by size:
3261 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3888 493
Size: 711 2684 2702
Fragments arranged by size:
2702 2684 711
BsaBI GATnn'nnATC
Cuts at: 6091 6091
Size: 6097
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2083 2563 3904 4286
Size: 53 83 186 936 550 480 1341 382
Cuts at: 4286 4921 275
Size: 635 1451
Fragments arranged by size:
1451 1341 936 635 550 480 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3315 4530 4928 5202 5349 5760 3315
Size: 1215 398 274 147 411 3652
Fragments arranged by size:
3652 1215 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2307 2337 3095 3125 1178
Size: 30 165 30 904 30 758 30 4150
Fragments arranged by size:
4150 904 758 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6097
BseYI C'CCAG_C
Cuts at: 2062 2122 2713 2798 3360 5300 5796 2062
Size: 60 591 85 562 1940 496 2363
Fragments arranged by size:
2363 1940 591 562 496 85 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3019 3086 3386 1163
Size: 573 1283 67 300 3874
Fragments arranged by size:
3874 1283 573 300 67
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2777 3547 4308 5336 1540
Size: 275 186 776 770 761 1028 2301
Fragments arranged by size:
2301 1028 776 770 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2235 3378 4158 4243 5314 2235
Size: 1143 780 85 1071 3018
Fragments arranged by size:
3018 1143 1071 780 85
BsmI GAATG_Cn'
Cuts at: 3608 3701 3608
Size: 93 6004
BsmBI CGTCTCn'nnnn_
Cuts at: 2825 2825
Size: 6097
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2235 2298 2743 3378 3416 4158 4243
Size: 399 445 63 445 635 38 742 85
Cuts at: 4243 5314 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 635 445 445 399 85
63 38
BspEI T'CCGG_A
Cuts at: 3315 5760 3315
Size: 2445 3652
BspHI T'CATG_A
Cuts at: 2706 3986 5716 2706
Size: 1280 1730 3087
Fragments arranged by size:
3087 1730 1280
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2697 3135 3333 3452 844
Size: 1853 438 198 119 3489
Fragments arranged by size:
3489 1853 438 198 119
BsrBI CCG'CTC
Cuts at: 1394 1823 2745 2773 2791 2830 3984 1394
Size: 429 922 28 18 39 1154 3507
Fragments arranged by size:
3507 1154 922 429 39 28 18
BsrDI GCAATG_nn'
Cuts at: 65 1248 2415 4592 4766 65
Size: 1183 1167 2177 174 1396
Fragments arranged by size:
2177 1396 1183 1167 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2697 4741 4928 1700
Size: 110 887 2044 187 2869
Fragments arranged by size:
2869 2044 887 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6097
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6097
BssSI C'ACGA_G
Cuts at: 2854 4157 5169 2854
Size: 1303 1012 3782
Fragments arranged by size:
3782 1303 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3373 794
Size: 2579 3518
BstBI TT'CG_AA
Cuts at: 4901 4901
Size: 6097
BstEII G'GTnAC_C
Cuts at: 1184 2191 1184
Size: 1007 5090
BstXI CCAn_nnnn'nTGG
Cuts at: 2890 3121 3139 2890
Size: 231 18 5848
Fragments arranged by size:
5848 231 18
BstYI r'GATC_y
Cuts at: 1972 3427 3794 4179 4196 5648 5734 5746 6092
Size: 1455 367 385 17 1452 86 12 346
Cuts at: 6092 1972
Size: 1977
Fragments arranged by size:
1977 1455 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2754 2754
Size: 6097
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4606
Fragments arranged by size:
4606 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5358
Fragments arranged by size:
5358 683 56
BtsI GCAGTG_nn'
Cuts at: 3613 4407 4427 3613
Size: 794 20 5283
Fragments arranged by size:
5283 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6097
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6062
DraI TTT'AAA
Cuts at: 2671 3401 2671
Size: 730 5367
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3092 5104 809
Size: 507 1776 2012 1802
Fragments arranged by size:
2012 1802 1776 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2172 2535 2831
Size: 54 1425 50 275 18 342 363 296
Cuts at: 2831 3544 4433 6062 8
Size: 713 889 1629 43
Fragments arranged by size:
1629 1425 889 713 363 342 296 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3544 1537
Size: 275 1732 4090
Fragments arranged by size:
4090 1732 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2708 4027 1353
Size: 1355 1319 3423
Fragments arranged by size:
3423 1355 1319
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2630 3098 4684 5058 5204 387
Size: 1064 138 1041 468 1586 374 146 1280
Fragments arranged by size:
1586 1280 1064 1041 468 374 146 138
EcoICRI GAG'CTC
Cuts at: 2233 2233
Size: 6097
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2261 2315 2375 3068 3097 4160 4757
Size: 393 629 54 60 693 29 1063 597
Cuts at: 4757 5544 5812 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1063 787 693 629 597 393 268
60 54 29
EcoO109I rG'GnC_Cy
Cuts at: 2295 2739 2758 2972 2295
Size: 444 19 214 5420
Fragments arranged by size:
5420 444 214 19
EcoRI G'AATT_C
Cuts at: 2250 3406 3784 2250
Size: 1156 378 4563
Fragments arranged by size:
4563 1156 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2345 2345
Size: 6097
FspI TGC'GCA
Cuts at: 4603 4603
Size: 6097
HaeII r_GCGC'y
Cuts at: 1678 2036 2080 2086 2566 5244 1678
Size: 358 44 6 480 2678 2531
Fragments arranged by size:
2678 2531 480 358 44 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4738 4770 4812 4844 1373
Size: 32 135 32 3166 32 42 32 2626
Fragments arranged by size:
3166 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2268 3441 3687 669
Size: 988 281 330 1173 246 3079
Fragments arranged by size:
3079 1173 988 330 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6097
HpaI GTT'AAC
Cuts at: 3687 3687
Size: 6097
KasI G'GCGC_C
Cuts at: 2082 2562 2082
Size: 480 5617
KpnI G_GTAC'C
Cuts at: 3422 3422
Size: 6097
MfeI C'AATT_G
Cuts at: 3696 3696
Size: 6097
MluI A'CGCG_T
Cuts at: 2816 5973 2816
Size: 3157 2940
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5211 5395 1305
Size: 3906 184 2007
Fragments arranged by size:
3906 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2174 2537 10
Size: 54 1425 343 342 363 3570
Fragments arranged by size:
3570 1425 363 343 342 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2478 3137 4055 4414 4573 518
Size: 648 1312 659 918 359 159 2042
Fragments arranged by size:
2042 1312 918 659 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2053 3262 3513 4190 5338 5583 1606
Size: 447 1209 251 677 1148 245 2120
Fragments arranged by size:
2120 1209 1148 677 447 251 245
NarI GG'CG_CC
Cuts at: 2083 2563 2083
Size: 480 5617
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4606
Fragments arranged by size:
4606 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6097
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6097
NotI GC'GGCC_GC
Cuts at: 3544 3544
Size: 6097
NspI r_CATG'y
Cuts at: 1132 1229 2108 2725 3151 3455 5000 1132
Size: 97 879 617 426 304 1545 2229
Fragments arranged by size:
2229 1545 879 617 426 304 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6097
PciI A'CATG_T
Cuts at: 3147 4996 3147
Size: 1849 4248
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6097
PmeI GTTT'AAAC
Cuts at: 3401 3401
Size: 6097
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4166 4198 5679 5711 4166
Size: 32 1481 32 4552
Fragments arranged by size:
4552 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2758 2758
Size: 6097
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6097
PsiI TTA'TAA
Cuts at: 3667 3667
Size: 6097
PspOMI G'GGCC_C
Cuts at: 2294 2739 2294
Size: 445 5652
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2537 2569 2537
Size: 32 6065
PstI C_TGCA'G
Cuts at: 830 2694 3267 3449 830
Size: 1864 573 182 3478
Fragments arranged by size:
3478 1864 573 182
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6097
PvuII CAG'CTG
Cuts at: 3262 3262
Size: 6097
SacI G_AGCT'C
Cuts at: 2235 2235
Size: 6097
SalI G'TCGA_C
Cuts at: 1936 3439 1936
Size: 1503 4594
SapI GCTCTTCn'nnn_
Cuts at: 2708 2708
Size: 6097
SbfI CC_TGCA'GG
Cuts at: 3449 3449
Size: 6097
ScaI AGT'ACT
Cuts at: 4345 6057 4345
Size: 1712 4385
SfcI C'TryA_G
Cuts at: 826 1045 2690 3263 3445 4580 5261 5452 826
Size: 219 1645 573 182 1135 681 191 1471
Fragments arranged by size:
1645 1471 1135 681 573 219 191 182
SfoI GGC'GCC
Cuts at: 2084 2564 2084
Size: 480 5617
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6097
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6097
SmaI CCC'GGG
Cuts at: 1872 3424 1872
Size: 1552 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4201 5102 5364 820
Size: 197 926 2258 901 262 1553
Fragments arranged by size:
2258 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3888 493
Size: 3395 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6097
SphI G_CATG'C
Cuts at: 1132 2725 3455 1132
Size: 1593 730 3774
Fragments arranged by size:
3774 1593 730
SspI AAT'ATT
Cuts at: 5 52 4021 5
Size: 47 3969 2081
Fragments arranged by size:
3969 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6097
StyI C'CwwG_G
Cuts at: 513 1065 2004 3536 513
Size: 552 939 1532 3074
Fragments arranged by size:
3074 1532 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3080 3352 4124 4141 4294 4479 4924
Size: 1255 1500 272 772 17 153 185 445
Cuts at: 4924 325
Size: 1498
Fragments arranged by size:
1500 1498 1255 772 445 272 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2326 2340 4343
Size: 274 80 33 51 1443 349 14 2003
Cuts at: 4343 6055 96
Size: 1712 138
Fragments arranged by size:
2003 1712 1443 349 274 138 80 51
33 14
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3095 3127 6057 6089 3095
Size: 32 2930 32 3103
Fragments arranged by size:
3103 2930 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2762 1316
Size: 1446 4651
XbaI T'CTAG_A
Cuts at: 3433 3433
Size: 6097
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2497 3139 2497
Size: 642 5455
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6097
XmaI C'CCGG_G
Cuts at: 1870 3422 1870
Size: 1552 4545
XmnI GAAnn'nnTTC
Cuts at: 3014 4226 3014
Size: 1212 4885
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3905 276
Size: 53 83 186 936 2371 2468
Fragments arranged by size:
2468 2371 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstZ17I DraIII EcoNI EcoRV FseI FspAI
NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI
RsrII SacII SanDI SexAI SfiI SrfI SwaI