Restriction map for
pFN21AE6438
(Circular) (Six-base) MAPSORT of: pFN21AE6438.seq Check: 3938 from: 1 to: 6103
pFN21AE6438 6103 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3913 278
Size: 53 83 186 936 2377 2468
Fragments arranged by size:
2468 2377 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3446 1937
Size: 1509 4594
Acc65I G'GTAC_C
Cuts at: 3424 3424
Size: 6103
AclI AA'CG_TT
Cuts at: 1563 4230 4603 1563
Size: 2667 373 3063
Fragments arranged by size:
3063 2667 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2126 2255 2593 2846 3015 4166 5550 5818
Size: 494 129 338 253 169 1151 1384 268
Cuts at: 5818 1632
Size: 1917
Fragments arranged by size:
1917 1384 1151 494 338 268 253 169
129
AflII C'TTAA_G
Cuts at: 820 1017 3274 820
Size: 197 2257 3649
Fragments arranged by size:
3649 2257 197
AflIII A'CryG_T
Cuts at: 1203 5002 5979 1203
Size: 3799 977 1327
Fragments arranged by size:
3799 1327 977
AgeI A'CCGG_T
Cuts at: 4934 4934
Size: 6103
AhdI GACnn_n'nnGTC
Cuts at: 4832 4832
Size: 6103
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6103
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6103
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2537 2569 2537
Size: 32 6071
AlwNI CAG_nnn'CTG
Cuts at: 2061 3846 5418 2061
Size: 1785 1572 2746
Fragments arranged by size:
2746 1785 1572
ApaLI G'TGCA_C
Cuts at: 4160 5316 4160
Size: 1156 4947
ApoI r'AATT_y
Cuts at: 1493 2382 2578 2935 2954 3412 3638 3790 5899
Size: 889 196 357 19 458 226 152 2109
Cuts at: 5899 1493
Size: 1697
Fragments arranged by size:
2109 1697 889 458 357 226 196 152
19
AseI AT'TA_AT
Cuts at: 160 1032 4657 160
Size: 872 3625 1606
Fragments arranged by size:
3625 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6103
AvaI C'yCGr_G
Cuts at: 1870 1943 3428 1870
Size: 73 1485 4545
Fragments arranged by size:
4545 1485 73
BamHI G'GATC_C
Cuts at: 3433 3800 3433
Size: 367 5736
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3424 4879 618
Size: 325 217 627 1637 1455 1842
Fragments arranged by size:
1842 1637 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3422 1391
Size: 2031 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2599 3003 928
Size: 971 700 404 4028
Fragments arranged by size:
4028 971 700 404
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4294 4328 1903
Size: 34 2357 34 3678
Fragments arranged by size:
3678 2357 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3070 3995 5205 3070
Size: 925 1210 3968
Fragments arranged by size:
3968 1210 925
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6103
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4714 136
Size: 107 122 71 631 605 3042 1525
Fragments arranged by size:
3042 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2491 2725 6098 2491
Size: 234 3373 2496
Fragments arranged by size:
3373 2496 234
BlpI GC'TnA_GC
Cuts at: 3520 3520
Size: 6103
Bme1580I G_kGCm'C
Cuts at: 1790 4164 5320 1790
Size: 2374 1156 2573
Fragments arranged by size:
2573 2374 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6103
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3834 4792 442
Size: 416 53 83 399 24 2417 958 1753
Fragments arranged by size:
2417 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6103
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2318 3071 4763 1239
Size: 1079 753 1692 2579
Fragments arranged by size:
2579 1692 1079 753
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2750 3290 4228 5093 5391 2750
Size: 540 938 865 298 3462
Fragments arranged by size:
3462 938 865 540 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4766 882
Size: 617 3267 2219
Fragments arranged by size:
3267 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3355 3894 493
Size: 711 2151 539 2702
Fragments arranged by size:
2702 2151 711 539
BsaBI GATnn'nnATC
Cuts at: 6097 6097
Size: 6103
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3910 4292 4927 275
Size: 53 83 186 936 2377 382 635 1451
Fragments arranged by size:
2377 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4536 4934 5208 5355 5766 4536
Size: 398 274 147 411 4873
Fragments arranged by size:
4873 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2537 2567 1178
Size: 30 165 30 1134 30 4714
Fragments arranged by size:
4714 1134 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6103
BseYI C'CCAG_C
Cuts at: 3153 5306 5802 3153
Size: 2153 496 3454
Fragments arranged by size:
3454 2153 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3005 1163
Size: 573 1269 4261
Fragments arranged by size:
4261 1269 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3553 4314 5342 1540
Size: 275 186 1552 761 1028 2301
Fragments arranged by size:
2301 1552 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2231 4164 4249 5320 2231
Size: 1933 85 1071 3014
Fragments arranged by size:
3014 1933 1071 85
BsmI GAATG_Cn'
Cuts at: 2354 3279 3614 3707 2354
Size: 925 335 93 4750
Fragments arranged by size:
4750 925 335 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2231 3422 4164 4249 5320 1391
Size: 399 441 1191 742 85 1071 2174
Fragments arranged by size:
2174 1191 1071 742 441 399 85
BspEI T'CCGG_A
Cuts at: 5766 5766
Size: 6103
BspHI T'CATG_A
Cuts at: 2187 2959 3229 3992 5722 2187
Size: 772 270 763 1730 2568
Fragments arranged by size:
2568 1730 772 763 270
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2343 2402 3022 3458 844
Size: 1499 59 620 436 3489
Fragments arranged by size:
3489 1499 620 436 59
BsrBI CCG'CTC
Cuts at: 1394 1823 3990 1394
Size: 429 2167 3507
Fragments arranged by size:
3507 2167 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2741 4598 4772 65
Size: 1183 1493 1857 174 1396
Fragments arranged by size:
1857 1493 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4747 4934 1700
Size: 110 2937 187 2869
Fragments arranged by size:
2937 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6103
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6103
BssSI C'ACGA_G
Cuts at: 4163 5175 4163
Size: 1012 5091
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2358 794
Size: 1564 4539
BstBI TT'CG_AA
Cuts at: 4907 4907
Size: 6103
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6103
BstYI r'GATC_y
Cuts at: 1972 2491 2725 3309 3433 3800 4185 4202 5654
Size: 519 234 584 124 367 385 17 1452
Cuts at: 5654 5740 5752 6098 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 584 519 385 367 346 234
124 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2869 513
Size: 552 1804 3747
Fragments arranged by size:
3747 1804 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5364
Fragments arranged by size:
5364 683 56
BtsI GCAGTG_nn'
Cuts at: 2059 3619 4413 4433 2059
Size: 1560 794 20 3729
Fragments arranged by size:
3729 1560 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6103
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6068
DraI TTT'AAA
Cuts at: 3394 3407 3394
Size: 13 6090
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5110 809
Size: 507 3794 1802
Fragments arranged by size:
3794 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3076 3550 4439
Size: 54 1425 50 275 18 1246 474 889
Cuts at: 4439 6068 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1246 889 474 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3550 1537
Size: 275 1738 4090
Fragments arranged by size:
4090 1738 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2102 2139 4033 1353
Size: 749 37 1894 3423
Fragments arranged by size:
3423 1894 749 37
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4690 5064 5210 387
Size: 1064 138 3101 374 146 1280
Fragments arranged by size:
3101 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2126 2255 2318 2593 2846 3015 3071
Size: 393 494 129 63 275 253 169 56
Cuts at: 3071 4166 4763 5550 5818 1239
Size: 1095 597 787 268 1524
Fragments arranged by size:
1524 1095 787 597 494 393 275 268
253 169 129 63 56
EcoO109I rG'GnC_Cy
Cuts at: 2263 3178 2263
Size: 915 5188
EcoRI G'AATT_C
Cuts at: 2578 3412 3790 2578
Size: 834 378 4891
Fragments arranged by size:
4891 834 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2744 2744
Size: 6103
FspI TGC'GCA
Cuts at: 2065 4609 2065
Size: 2544 3559
HaeII r_GCGC'y
Cuts at: 1678 5250 1678
Size: 3572 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4744 4776 4818 4850 1373
Size: 32 135 32 3172 32 42 32 2626
Fragments arranged by size:
3172 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2565 3447 3693 669
Size: 988 281 627 882 246 3079
Fragments arranged by size:
3079 988 882 627 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6103
HpaI GTT'AAC
Cuts at: 3693 3693
Size: 6103
KpnI G_GTAC'C
Cuts at: 3428 3428
Size: 6103
MfeI C'AATT_G
Cuts at: 2531 3702 2531
Size: 1171 4932
MluI A'CGCG_T
Cuts at: 5979 5979
Size: 6103
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5217 5401 1305
Size: 3912 184 2007
Fragments arranged by size:
3912 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3078 10
Size: 54 1425 343 1246 3035
Fragments arranged by size:
3035 1425 1246 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4061 4420 4579 518
Size: 648 2895 359 159 2042
Fragments arranged by size:
2895 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2764 3519 4196 5344 5589 1606
Size: 1158 755 677 1148 245 2120
Fragments arranged by size:
2120 1158 1148 755 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5551
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6103
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6103
NotI GC'GGCC_GC
Cuts at: 3550 3550
Size: 6103
NspI r_CATG'y
Cuts at: 1132 1229 3461 5006 1132
Size: 97 2232 1545 2229
Fragments arranged by size:
2232 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6103
PciI A'CATG_T
Cuts at: 5002 5002
Size: 6103
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6103
PmeI GTTT'AAAC
Cuts at: 3407 3407
Size: 6103
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2537 2569 4172 4204 5685 5717 2537
Size: 32 1603 32 1481 32 2923
Fragments arranged by size:
2923 1603 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6103
PsiI TTA'TAA
Cuts at: 3673 3673
Size: 6103
PstI C_TGCA'G
Cuts at: 830 3455 830
Size: 2625 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6103
PvuII CAG'CTG
Cuts at: 2764 2764
Size: 6103
SalI G'TCGA_C
Cuts at: 1936 3445 1936
Size: 1509 4594
SbfI CC_TGCA'GG
Cuts at: 3455 3455
Size: 6103
ScaI AGT'ACT
Cuts at: 4351 6063 4351
Size: 1712 4391
SfcI C'TryA_G
Cuts at: 826 1045 3371 3451 4586 5267 5458 826
Size: 219 2326 80 1135 681 191 1471
Fragments arranged by size:
2326 1471 1135 681 219 191 80
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6103
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6103
SmaI CCC'GGG
Cuts at: 1872 3430 1872
Size: 1558 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2729 3274 3305 4207 5108 5370
Size: 197 926 786 545 31 902 901 262
Cuts at: 5370 820
Size: 1553
Fragments arranged by size:
1553 926 902 901 786 545 262 197
31
SnaBI TAC'GTA
Cuts at: 493 3355 3894 493
Size: 2862 539 2702
Fragments arranged by size:
2862 2702 539
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6103
SphI G_CATG'C
Cuts at: 1132 3461 1132
Size: 2329 3774
SspI AAT'ATT
Cuts at: 5 52 3241 4027 5
Size: 47 3189 786 2081
Fragments arranged by size:
3189 2081 786 47
StuI AGG'CCT
Cuts at: 1517 2877 1517
Size: 1360 4743
StyI C'CwwG_G
Cuts at: 513 1065 3221 3542 513
Size: 552 2156 321 3074
Fragments arranged by size:
3074 2156 552 321
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2083 3145 4130 4147 4300 4485 4930
Size: 1255 503 1062 985 17 153 185 445
Cuts at: 4930 325
Size: 1498
Fragments arranged by size:
1498 1255 1062 985 503 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4349 6061 96
Size: 274 80 33 51 1443 2372 1712 138
Fragments arranged by size:
2372 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3294 3326 6063 6095 3294
Size: 32 2737 32 3302
Fragments arranged by size:
3302 2737 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6103
XbaI T'CTAG_A
Cuts at: 3439 3439
Size: 6103
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6103
XmaI C'CCGG_G
Cuts at: 1870 3428 1870
Size: 1558 4545
XmnI GAAnn'nnTTC
Cuts at: 3241 4232 3241
Size: 991 5112
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3911 276
Size: 53 83 186 936 2377 2468
Fragments arranged by size:
2468 2377 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI