Restriction map for
pFN21AE6435
(Circular) (Six-base) MAPSORT of: pFN21AE6435.seq Check: 392 from: 1 to: 6124
pFN21AE6435 6124 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2136 2136
Size: 6124
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3934 278
Size: 53 83 186 936 2398 2468
Fragments arranged by size:
2468 2398 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3467 1937
Size: 1530 4594
Acc65I G'GTAC_C
Cuts at: 3445 3445
Size: 6124
AclI AA'CG_TT
Cuts at: 1563 4251 4624 1563
Size: 2688 373 3063
Fragments arranged by size:
3063 2688 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2068 2296 2511 2690 4187 5571 5839 1632
Size: 436 228 215 179 1497 1384 268 1917
Fragments arranged by size:
1917 1497 1384 436 268 228 215 179
AfeI AGC'GCT
Cuts at: 2144 2144
Size: 6124
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5927
AflIII A'CryG_T
Cuts at: 1203 2266 5023 6000 1203
Size: 1063 2757 977 1327
Fragments arranged by size:
2757 1327 1063 977
AgeI A'CCGG_T
Cuts at: 4955 4955
Size: 6124
AhdI GACnn_n'nnGTC
Cuts at: 4853 4853
Size: 6124
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6124
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6124
AlwNI CAG_nnn'CTG
Cuts at: 3238 3867 5439 3238
Size: 629 1572 3923
Fragments arranged by size:
3923 1572 629
ApaLI G'TGCA_C
Cuts at: 4181 5337 4181
Size: 1156 4968
ApoI r'AATT_y
Cuts at: 1493 2690 2700 2973 3020 3190 3245 3433 3659
Size: 1197 10 273 47 170 55 188 226
Cuts at: 3659 3811 5920 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1197 273 226 188 170 152
55 47 10
AseI AT'TA_AT
Cuts at: 160 1032 4678 160
Size: 872 3646 1606
Fragments arranged by size:
3646 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6124
AvaI C'yCGr_G
Cuts at: 1870 1943 3449 1870
Size: 73 1506 4545
Fragments arranged by size:
4545 1506 73
BamHI G'GATC_C
Cuts at: 2179 3454 3821 2179
Size: 1275 367 4482
Fragments arranged by size:
4482 1275 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3445 4900 618
Size: 325 217 627 1658 1455 1842
Fragments arranged by size:
1842 1658 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2106 3443 1391
Size: 715 1337 4072
Fragments arranged by size:
4072 1337 715
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2578 2661 2852 928
Size: 971 679 83 191 4200
Fragments arranged by size:
4200 971 679 191 83
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4315 4349 1903
Size: 34 2378 34 3678
Fragments arranged by size:
3678 2378 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2071 2396 4016 5226 2071
Size: 325 1620 1210 2969
Fragments arranged by size:
2969 1620 1210 325
BclI T'GATC_A
Cuts at: 1548 2229 1548
Size: 681 5443
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4735 136
Size: 107 122 71 631 605 3063 1525
Fragments arranged by size:
3063 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2118 6119 2118
Size: 4001 2123
BlpI GC'TnA_GC
Cuts at: 3541 3541
Size: 6124
Bme1580I G_kGCm'C
Cuts at: 1790 4185 5341 1790
Size: 2395 1156 2573
Fragments arranged by size:
2573 2395 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6124
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2167 3855 4813
Size: 416 53 83 399 24 750 1688 958
Cuts at: 4813 442
Size: 1753
Fragments arranged by size:
1753 1688 958 750 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3050 1056
Size: 1994 4130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4784 1239
Size: 3545 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2062 2365 2764 4249 5114 5412 2062
Size: 303 399 1485 865 298 2774
Fragments arranged by size:
2774 1485 865 399 303 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4787 882
Size: 617 3288 2219
Fragments arranged by size:
3288 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3915 493
Size: 711 2711 2702
Fragments arranged by size:
2711 2702 711
BsaBI GATnn'nnATC
Cuts at: 6118 6118
Size: 6124
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3931 4313 4948 275
Size: 53 83 186 936 2398 382 635 1451
Fragments arranged by size:
2398 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4557 4955 5229 5376 5787 4557
Size: 398 274 147 411 4894
Fragments arranged by size:
4894 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5899
Fragments arranged by size:
5899 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2060 1186
Size: 874 5250
BseYI C'CCAG_C
Cuts at: 5327 5823 5327
Size: 496 5628
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2613 1163
Size: 573 877 4674
Fragments arranged by size:
4674 877 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2037 2839 3574 4335 5363 1540
Size: 275 186 36 802 735 761 1028 2301
Fragments arranged by size:
2301 1028 802 761 735 275 186 36
BsiHKAI G_wGCw'C
Cuts at: 4185 4270 5341 4185
Size: 85 1071 4968
Fragments arranged by size:
4968 1071 85
BsmI GAATG_Cn'
Cuts at: 3635 3728 3635
Size: 93 6031
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2106 3443 4185 4270 5341 1391
Size: 399 316 1337 742 85 1071 2174
Fragments arranged by size:
2174 1337 1071 742 399 316 85
BspEI T'CCGG_A
Cuts at: 5787 5787
Size: 6124
BspHI T'CATG_A
Cuts at: 2848 4013 5743 2848
Size: 1165 1730 3229
Fragments arranged by size:
3229 1730 1165
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2136 2245 3479 844
Size: 1292 109 1234 3489
Fragments arranged by size:
3489 1292 1234 109
BsrBI CCG'CTC
Cuts at: 1394 1823 4011 1394
Size: 429 2188 3507
Fragments arranged by size:
3507 2188 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2604 4619 4793 65
Size: 1183 1356 2015 174 1396
Fragments arranged by size:
2015 1396 1356 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4768 4955 1700
Size: 110 2958 187 2869
Fragments arranged by size:
2958 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6124
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6124
BssSI C'ACGA_G
Cuts at: 4184 5196 4184
Size: 1012 5112
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6124
BstBI TT'CG_AA
Cuts at: 4928 4928
Size: 6124
BstEII G'GTnAC_C
Cuts at: 1184 2989 1184
Size: 1805 4319
BstYI r'GATC_y
Cuts at: 1972 2118 2179 2247 2468 3454 3821 4206 4223
Size: 146 61 68 221 986 367 385 17
Cuts at: 4223 5675 5761 5773 6119 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 986 385 367 346 221 146
86 68 61 17 12
Bsu36I CC'TnA_GG
Cuts at: 2133 2133
Size: 6124
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4633
Fragments arranged by size:
4633 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5385
Fragments arranged by size:
5385 683 56
BtsI GCAGTG_nn'
Cuts at: 3640 4434 4454 3640
Size: 794 20 5310
Fragments arranged by size:
5310 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6124
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6089
DraI TTT'AAA
Cuts at: 2737 3428 2737
Size: 691 5433
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5131 809
Size: 507 3815 1802
Fragments arranged by size:
3815 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 3389 3571
Size: 54 1425 50 275 18 204 1355 182
Cuts at: 3571 4460 6089 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1355 889 275 204 182 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2034 3571 1537
Size: 275 222 1537 4090
Fragments arranged by size:
4090 1537 275 222
EarI CTCTTCn'nnn_
Cuts at: 1353 2078 2315 4054 1353
Size: 725 237 1739 3423
Fragments arranged by size:
3423 1739 725 237
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2068 4711 5085 5231 387
Size: 1064 138 479 2643 374 146 1280
Fragments arranged by size:
2643 1280 1064 479 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2068 2296 2511 2690 4187 4784 5571
Size: 393 436 228 215 179 1497 597 787
Cuts at: 5571 5839 1239
Size: 268 1524
Fragments arranged by size:
1524 1497 787 597 436 393 268 228
215 179
EcoNI CCTnn'n_nnAGG
Cuts at: 2132 2673 2132
Size: 541 5583
EcoRI G'AATT_C
Cuts at: 2690 2973 3433 3811 2690
Size: 283 460 378 5003
Fragments arranged by size:
5003 460 378 283
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2678 2876 2678
Size: 198 5926
FspI TGC'GCA
Cuts at: 4630 4630
Size: 6124
HaeII r_GCGC'y
Cuts at: 1678 2146 5271 1678
Size: 468 3125 2531
Fragments arranged by size:
3125 2531 468
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4765 4797 4839 4871 1373
Size: 32 135 32 3193 32 42 32 2626
Fragments arranged by size:
3193 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2403 2619 3468 3714 669
Size: 988 281 465 216 849 246 3079
Fragments arranged by size:
3079 988 849 465 281 246 216
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6124
HpaI GTT'AAC
Cuts at: 2619 3714 2619
Size: 1095 5029
KpnI G_GTAC'C
Cuts at: 3449 3449
Size: 6124
MfeI C'AATT_G
Cuts at: 2220 3723 2220
Size: 1503 4621
MluI A'CGCG_T
Cuts at: 6000 6000
Size: 6124
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2450 3128 5238 5422 1305
Size: 1145 678 2110 184 2007
Fragments arranged by size:
2110 2007 1145 678 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3391 10
Size: 54 1425 343 1559 2743
Fragments arranged by size:
2743 1559 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2285 4082 4441 4600 518
Size: 648 1119 1797 359 159 2042
Fragments arranged by size:
2042 1797 1119 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2014 3540 4217 5365 5610 1606
Size: 408 1526 677 1148 245 2120
Fragments arranged by size:
2120 1526 1148 677 408 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4633
Fragments arranged by size:
4633 939 552
NdeI CA'TA_TG
Cuts at: 387 3206 387
Size: 2819 3305
NheI G'CTAG_C
Cuts at: 1052 3046 1052
Size: 1994 4130
NotI GC'GGCC_GC
Cuts at: 3571 3571
Size: 6124
NspI r_CATG'y
Cuts at: 1132 1229 2270 3482 5027 1132
Size: 97 1041 1212 1545 2229
Fragments arranged by size:
2229 1545 1212 1041 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6124
PciI A'CATG_T
Cuts at: 2266 5023 2266
Size: 2757 3367
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6124
PmeI GTTT'AAAC
Cuts at: 3428 3428
Size: 6124
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4193 4225 5706 5738 4193
Size: 32 1481 32 4579
Fragments arranged by size:
4579 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6124
PsiI TTA'TAA
Cuts at: 3694 3694
Size: 6124
PstI C_TGCA'G
Cuts at: 830 3476 830
Size: 2646 3478
PvuI CG_AT'CG
Cuts at: 2001 2839 2001
Size: 838 5286
PvuII CAG'CTG
Cuts at: 2014 2014
Size: 6124
SalI G'TCGA_C
Cuts at: 1936 3466 1936
Size: 1530 4594
SbfI CC_TGCA'GG
Cuts at: 3476 3476
Size: 6124
ScaI AGT'ACT
Cuts at: 4372 6084 4372
Size: 1712 4412
SexAI A'CCwGG_T
Cuts at: 2211 2211
Size: 6124
SfcI C'TryA_G
Cuts at: 826 1045 3472 4607 5288 5479 826
Size: 219 2427 1135 681 191 1471
Fragments arranged by size:
2427 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6124
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6124
SmaI CCC'GGG
Cuts at: 1872 3451 1872
Size: 1579 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2041 2380 2743 4228 5129 5391
Size: 197 926 98 339 363 1485 901 262
Cuts at: 5391 820
Size: 1553
Fragments arranged by size:
1553 1485 926 901 363 339 262 197
98
SnaBI TAC'GTA
Cuts at: 493 3915 493
Size: 3422 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6124
SphI G_CATG'C
Cuts at: 1132 3482 1132
Size: 2350 3774
SspI AAT'ATT
Cuts at: 5 52 3116 4048 5
Size: 47 3064 932 2081
Fragments arranged by size:
3064 2081 932 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6124
StyI C'CwwG_G
Cuts at: 513 1065 2004 2536 3563 513
Size: 552 939 532 1027 3074
Fragments arranged by size:
3074 1027 939 552 532
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4151 4168 4321 4506 4951 325
Size: 1255 2571 17 153 185 445 1498
Fragments arranged by size:
2571 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4370 6082 96
Size: 274 80 33 51 1443 2393 1712 138
Fragments arranged by size:
2393 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6084 6116 6084
Size: 32 6092
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6124
XbaI T'CTAG_A
Cuts at: 3460 3460
Size: 6124
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6124
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6124
XmaI C'CCGG_G
Cuts at: 1870 3449 1870
Size: 1579 4545
XmnI GAAnn'nnTTC
Cuts at: 4253 4253
Size: 6124
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3932 276
Size: 53 83 186 936 2398 2468
Fragments arranged by size:
2468 2398 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI