Restriction map for
pFN21AE6434
(Circular) (Six-base) MAPSORT of: pFN21AE6434.seq Check: 2542 from: 1 to: 6349
pFN21AE6434 6349 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2020 2020
Size: 6349
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4159 278
Size: 53 83 186 936 2623 2468
Fragments arranged by size:
2623 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3183 3692 1937
Size: 1246 509 4594
Fragments arranged by size:
4594 1246 509
Acc65I G'GTAC_C
Cuts at: 2757 3670 2757
Size: 913 5436
AclI AA'CG_TT
Cuts at: 1563 3648 4476 4849 1563
Size: 2085 828 373 3063
Fragments arranged by size:
3063 2085 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2161 2929 3348 3351 3596 4412 5796 6064
Size: 529 768 419 3 245 816 1384 268
Cuts at: 6064 1632
Size: 1917
Fragments arranged by size:
1917 1384 816 768 529 419 268 245
3
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6152
AflIII A'CryG_T
Cuts at: 1203 3126 5248 6225 1203
Size: 1923 2122 977 1327
Fragments arranged by size:
2122 1923 1327 977
AgeI A'CCGG_T
Cuts at: 5180 5180
Size: 6349
AhdI GACnn_n'nnGTC
Cuts at: 5078 5078
Size: 6349
AleI CACnn'nnGTG
Cuts at: 1166 2148 1166
Size: 982 5367
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3281 1184
Size: 2097 4252
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2973 3005 2973
Size: 32 6317
AlwNI CAG_nnn'CTG
Cuts at: 2241 2829 4092 5664 2241
Size: 588 1263 1572 2926
Fragments arranged by size:
2926 1572 1263 588
ApaI G_GGCC'C
Cuts at: 2049 2049
Size: 6349
ApaLI G'TGCA_C
Cuts at: 4406 5562 4406
Size: 1156 5193
ApoI r'AATT_y
Cuts at: 1493 2372 3658 3884 4036 6145 1493
Size: 879 1286 226 152 2109 1697
Fragments arranged by size:
2109 1697 1286 879 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4903 160
Size: 872 3871 1606
Fragments arranged by size:
3871 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6349
AvaI C'yCGr_G
Cuts at: 1870 1943 2085 2214 3674 1870
Size: 73 142 129 1460 4545
Fragments arranged by size:
4545 1460 142 129 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2530 2563 2530
Size: 33 6316
BamHI G'GATC_C
Cuts at: 3679 4046 3679
Size: 367 5982
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2162 2757 2836 3524 3670
Size: 325 217 627 375 595 79 688 146
Cuts at: 3670 5125 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 688 627 595 375 325 217
146 79
BanII G_rGCy'C
Cuts at: 1391 2049 3437 3668 1391
Size: 658 1388 231 4072
Fragments arranged by size:
4072 1388 658 231
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3001 3602 928
Size: 971 1102 601 3675
Fragments arranged by size:
3675 1102 971 601
BbvCI CC'TCA_GC
Cuts at: 2822 2822
Size: 6349
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4540 4574 1903
Size: 34 2603 34 3678
Fragments arranged by size:
3678 2603 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4241 5451 4241
Size: 1210 5139
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6349
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3449 3515 4960
Size: 107 122 71 631 605 1777 66 1445
Cuts at: 4960 136
Size: 1525
Fragments arranged by size:
1777 1525 1445 631 605 122 107 71
66
BglII A'GATC_T
Cuts at: 6344 6344
Size: 6349
BlpI GC'TnA_GC
Cuts at: 2234 2623 3766 2234
Size: 389 1143 4817
Fragments arranged by size:
4817 1143 389
Bme1580I G_kGCm'C
Cuts at: 1790 2049 2155 2167 2340 2812 3242 3601 4410
Size: 259 106 12 173 472 430 359 809
Cuts at: 4410 5566 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 809 472 430 359 259 173
106 12
BmgBI CAC'GTC
Cuts at: 1318 3583 1318
Size: 2265 4084
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2395 4080 5038
Size: 416 53 83 399 24 978 1685 958
Cuts at: 5038 442
Size: 1753
Fragments arranged by size:
1753 1685 978 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2792 1056
Size: 1736 4613
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2045 2219 2336 2681 2687 2721 3313 5009
Size: 806 174 117 345 6 34 592 1696
Cuts at: 5009 1239
Size: 2579
Fragments arranged by size:
2579 1696 806 592 345 174 117 34
6
Bpu10I CC'TnA_GC
Cuts at: 2708 2822 2708
Size: 114 6235
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4474 5339 5637 4474
Size: 865 298 5186
Fragments arranged by size:
5186 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3563 5012 882
Size: 617 2064 1449 2219
Fragments arranged by size:
2219 2064 1449 617
BsaAI yAC'GTr
Cuts at: 493 1204 2542 4140 493
Size: 711 1338 1598 2702
Fragments arranged by size:
2702 1598 1338 711
BsaBI GATnn'nnATC
Cuts at: 6343 6343
Size: 6349
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2521 3208 4156 4538
Size: 53 83 186 936 988 687 948 382
Cuts at: 4538 5173 275
Size: 635 1451
Fragments arranged by size:
1451 988 948 936 687 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4782 5180 5454 5601 6012 4782
Size: 398 274 147 411 5119
Fragments arranged by size:
5119 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2654 2684 3015 3045 1178
Size: 30 165 30 1251 30 331 30 4482
Fragments arranged by size:
4482 1251 331 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2030 2368 1186
Size: 844 338 5167
Fragments arranged by size:
5167 844 338
BseYI C'CCAG_C
Cuts at: 2352 2557 3115 3235 3427 5552 6048 2352
Size: 205 558 120 192 2125 496 2653
Fragments arranged by size:
2653 2125 558 496 205 192 120
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2087 2832 3396 1163
Size: 573 351 745 564 4116
Fragments arranged by size:
4116 745 573 564 351
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3799 4560 5588 1540
Size: 275 186 1798 761 1028 2301
Fragments arranged by size:
2301 1798 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2688 3299 3387 4410 4495 5566 2688
Size: 611 88 1023 85 1071 3471
Fragments arranged by size:
3471 1071 1023 611 88 85
BsmI GAATG_Cn'
Cuts at: 3860 3953 3860
Size: 93 6256
BsmBI CGTCTCn'nnnn_
Cuts at: 3539 3539
Size: 6349
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2155 2167 2340 2688 2812 3242
Size: 399 259 106 12 173 348 124 430
Cuts at: 3242 3299 3387 3437 3601 3668 4410 4495 5566
Size: 57 88 50 164 67 742 85 1071
Cuts at: 5566 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 430 399 348 259 173
164 124 106 88 85 67 57 50
12
BspEI T'CCGG_A
Cuts at: 6012 6012
Size: 6349
BspHI T'CATG_A
Cuts at: 4238 5968 4238
Size: 1730 4619
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2020 2335 3628 3704 844
Size: 1176 315 1293 76 3489
Fragments arranged by size:
3489 1293 1176 315 76
BsrBI CCG'CTC
Cuts at: 1394 1823 4236 1394
Size: 429 2413 3507
Fragments arranged by size:
3507 2413 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2385 4844 5018 65
Size: 1183 1137 2459 174 1396
Fragments arranged by size:
2459 1396 1183 1137 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2462 3423 3521 4993 5180 1700
Size: 110 652 961 98 1472 187 2869
Fragments arranged by size:
2869 1472 961 652 187 110 98
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6349
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6349
BssSI C'ACGA_G
Cuts at: 4409 5421 4409
Size: 1012 5337
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6349
BstBI TT'CG_AA
Cuts at: 5153 5153
Size: 6349
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6349
BstXI CCAn_nnnn'nTGG
Cuts at: 2125 3178 2125
Size: 1053 5296
BstYI r'GATC_y
Cuts at: 1972 2407 3679 4046 4431 4448 5900 5986 5998
Size: 435 1272 367 385 17 1452 86 12
Cuts at: 5998 6344 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1272 435 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2305 2694 3026 513
Size: 552 939 301 389 332 3836
Fragments arranged by size:
3836 939 552 389 332 301
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2791 487
Size: 683 56 1565 4045
Fragments arranged by size:
4045 1565 683 56
BtsI GCAGTG_nn'
Cuts at: 2069 2099 3230 3315 3345 3479 3865 4659 4679
Size: 30 1131 85 30 134 386 794 20
Cuts at: 4679 2069
Size: 3739
Fragments arranged by size:
3739 1131 794 386 134 85 30 30
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6349
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6314
DraI TTT'AAA
Cuts at: 3653 3653
Size: 6349
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5356 809
Size: 507 4040 1802
Fragments arranged by size:
4040 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2924 2970 3796
Size: 54 1425 50 275 18 1094 46 826
Cuts at: 3796 4685 6314 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1094 889 826 275 54 50
46 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3796 1537
Size: 275 1984 4090
Fragments arranged by size:
4090 1984 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3615 4279 1353
Size: 2262 664 3423
Fragments arranged by size:
3423 2262 664
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4936 5310 5456 387
Size: 1064 138 3347 374 146 1280
Fragments arranged by size:
3347 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2045 2161 2219 2336 2681 2687 2721
Size: 393 413 116 58 117 345 6 34
Cuts at: 2721 2929 3313 3348 3351 3596 4412 5009 5796
Size: 208 384 35 3 245 816 597 787
Cuts at: 5796 6064 1239
Size: 268 1524
Fragments arranged by size:
1524 816 787 597 413 393 384 345
268 245 208 117 116 58 35 34
6 3
EcoO109I rG'GnC_Cy
Cuts at: 2046 2046
Size: 6349
EcoRI G'AATT_C
Cuts at: 2372 3658 4036 2372
Size: 1286 378 4685
Fragments arranged by size:
4685 1286 378
EcoRV GAT'ATC
Cuts at: 2495 2495
Size: 6349
FspI TGC'GCA
Cuts at: 4855 4855
Size: 6349
HaeII r_GCGC'y
Cuts at: 1678 5496 1678
Size: 3818 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3198 3230 3272 3304 4990
Size: 32 135 32 1626 32 42 32 1686
Cuts at: 4990 5022 5064 5096 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1686 1626 135 42 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3693 3939 669
Size: 988 281 1755 246 3079
Fragments arranged by size:
3079 1755 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3321 3488 748
Size: 2573 167 3609
Fragments arranged by size:
3609 2573 167
HpaI GTT'AAC
Cuts at: 3939 3939
Size: 6349
KpnI G_GTAC'C
Cuts at: 2761 3674 2761
Size: 913 5436
MfeI C'AATT_G
Cuts at: 3948 3948
Size: 6349
MluI A'CGCG_T
Cuts at: 6225 6225
Size: 6349
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2077 2526 3058 5463 5647 1305
Size: 772 449 532 2405 184 2007
Fragments arranged by size:
2405 2007 772 532 449 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4527
Fragments arranged by size:
4527 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2148 2187 2648 2651 4307 4666 4825
Size: 648 982 39 461 3 1656 359 159
Cuts at: 4825 518
Size: 2042
Fragments arranged by size:
2042 1656 982 648 461 359 159 39
3
MspA1I CmG'CkG
Cuts at: 1606 3142 3562 3765 4442 5590 5835 1606
Size: 1536 420 203 677 1148 245 2120
Fragments arranged by size:
2120 1536 1148 677 420 245 203
NaeI GCC'GGC
Cuts at: 3425 3523 3425
Size: 98 6251
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4858
Fragments arranged by size:
4858 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6349
NgoMIV G'CCGG_C
Cuts at: 3423 3521 3423
Size: 98 6251
NheI G'CTAG_C
Cuts at: 1052 2788 1052
Size: 1736 4613
NotI GC'GGCC_GC
Cuts at: 3796 3796
Size: 6349
NspI r_CATG'y
Cuts at: 1132 1229 3707 5252 1132
Size: 97 2478 1545 2229
Fragments arranged by size:
2478 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6349
PciI A'CATG_T
Cuts at: 5248 5248
Size: 6349
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3583 1108
Size: 2475 3874
PfoI T'CCnGG_A
Cuts at: 3290 3576 3290
Size: 286 6063
PmeI GTTT'AAAC
Cuts at: 3653 3653
Size: 6349
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4418 4450 5931 5963 4418
Size: 32 1481 32 4804
Fragments arranged by size:
4804 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6349
PsiI TTA'TAA
Cuts at: 3919 3919
Size: 6349
PspOMI G'GGCC_C
Cuts at: 2045 2045
Size: 6349
PstI C_TGCA'G
Cuts at: 830 2575 3701 830
Size: 1745 1126 3478
Fragments arranged by size:
3478 1745 1126
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6349
PvuII CAG'CTG
Cuts at: 3142 3562 3142
Size: 420 5929
SalI G'TCGA_C
Cuts at: 1936 3691 1936
Size: 1755 4594
SbfI CC_TGCA'GG
Cuts at: 3701 3701
Size: 6349
ScaI AGT'ACT
Cuts at: 4597 6309 4597
Size: 1712 4637
SfcI C'TryA_G
Cuts at: 826 1045 2571 3697 4832 5513 5704 826
Size: 219 1526 1126 1135 681 191 1471
Fragments arranged by size:
1526 1471 1135 1126 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6349
SgrAI Cr'CCGG_yG
Cuts at: 1700 2462 1700
Size: 762 5587
SmaI CCC'GGG
Cuts at: 1872 2216 3676 1872
Size: 344 1460 4545
Fragments arranged by size:
4545 1460 344
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4453 5354 5616 820
Size: 197 926 2510 901 262 1553
Fragments arranged by size:
2510 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4140 493
Size: 3647 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6349
SphI G_CATG'C
Cuts at: 1132 3707 1132
Size: 2575 3774
SspI AAT'ATT
Cuts at: 5 52 4273 5
Size: 47 4221 2081
Fragments arranged by size:
4221 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6349
StyI C'CwwG_G
Cuts at: 513 1065 2004 2866 3788 513
Size: 552 939 862 922 3074
Fragments arranged by size:
3074 939 922 862 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2824 4376 4393 4546 4731 5176 325
Size: 1255 1244 1552 17 153 185 445 1498
Fragments arranged by size:
1552 1498 1255 1244 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4595 6307 96
Size: 274 80 33 51 1443 2618 1712 138
Fragments arranged by size:
2618 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2091 2123 2399 2431 2654 2686 3015 3047 3455
Size: 32 276 32 223 32 329 32 408
Cuts at: 3455 3487 6309 6341 2091
Size: 32 2822 32 2099
Fragments arranged by size:
2822 2099 408 329 276 223 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6349
XbaI T'CTAG_A
Cuts at: 3685 3685
Size: 6349
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2281 3580 2281
Size: 1299 5050
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6349
XmaI C'CCGG_G
Cuts at: 1870 2214 3674 1870
Size: 344 1460 4545
Fragments arranged by size:
4545 1460 344
XmnI GAAnn'nnTTC
Cuts at: 2998 4478 2998
Size: 1480 4869
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4157 276
Size: 53 83 186 936 2623 2468
Fragments arranged by size:
2623 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BbeI BfrBI BplI BsiWI BstZ17I
Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI
NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI