Restriction map for
pFN21AE6432
(Circular) (Six-base) MAPSORT of: pFN21AE6432.seq Check: 4177 from: 1 to: 6376
pFN21AE6432 6376 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3580 3580
Size: 6376
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4186 278
Size: 53 83 186 936 2650 2468
Fragments arranged by size:
2650 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3719 1937
Size: 1782 4594
Acc65I G'GTAC_C
Cuts at: 3697 3697
Size: 6376
AclI AA'CG_TT
Cuts at: 1563 4503 4876 1563
Size: 2940 373 3063
Fragments arranged by size:
3063 2940 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2411 2461 4439 5823 6091 1632
Size: 779 50 1978 1384 268 1917
Fragments arranged by size:
1978 1917 1384 779 268 50
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6179
AflIII A'CryG_T
Cuts at: 1203 2274 5275 6252 1203
Size: 1071 3001 977 1327
Fragments arranged by size:
3001 1327 1071 977
AgeI A'CCGG_T
Cuts at: 5207 5207
Size: 6376
AhdI GACnn_n'nnGTC
Cuts at: 5105 5105
Size: 6376
AleI CACnn'nnGTG
Cuts at: 1166 2186 2429 3236 1166
Size: 1020 243 807 4306
Fragments arranged by size:
4306 1020 807 243
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2204 1184
Size: 1020 5356
AlwNI CAG_nnn'CTG
Cuts at: 2980 3025 3642 4119 5691 2980
Size: 45 617 477 1572 3665
Fragments arranged by size:
3665 1572 617 477 45
ApaLI G'TGCA_C
Cuts at: 2338 2863 2879 4433 5589 2338
Size: 525 16 1554 1156 3125
Fragments arranged by size:
3125 1554 1156 525 16
ApoI r'AATT_y
Cuts at: 1493 3685 3911 4063 6172 1493
Size: 2192 226 152 2109 1697
Fragments arranged by size:
2192 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4930 160
Size: 872 3898 1606
Fragments arranged by size:
3898 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6376
AvaI C'yCGr_G
Cuts at: 1870 1943 2064 2220 2962 3480 3498 3701 1870
Size: 73 121 156 742 518 18 203 4545
Fragments arranged by size:
4545 742 518 203 156 121 73 18
BamHI G'GATC_C
Cuts at: 3706 4073 3706
Size: 367 6009
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2029 3487 3580 3697 5152
Size: 325 217 627 242 1458 93 117 1455
Cuts at: 5152 618
Size: 1842
Fragments arranged by size:
1842 1458 1455 627 325 242 217 117
93
BanII G_rGCy'C
Cuts at: 1391 2040 2840 3097 3634 3695 1391
Size: 649 800 257 537 61 4072
Fragments arranged by size:
4072 800 649 537 257 61
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2206 2408 928
Size: 971 307 202 4896
Fragments arranged by size:
4896 971 307 202
BbvCI CC'TCA_GC
Cuts at: 3576 3576
Size: 6376
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3352 3386 4567 4601 1903
Size: 34 1415 34 1181 34 3678
Fragments arranged by size:
3678 1415 1181 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4268 5478 4268
Size: 1210 5166
BclI T'GATC_A
Cuts at: 1548 2563 1548
Size: 1015 5361
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4987 136
Size: 107 122 71 631 605 3315 1525
Fragments arranged by size:
3315 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3171 6371 3171
Size: 3200 3176
BlpI GC'TnA_GC
Cuts at: 3264 3793 3264
Size: 529 5847
Bme1580I G_kGCm'C
Cuts at: 1790 2342 2867 2883 3522 4437 5593 1790
Size: 552 525 16 639 915 1156 2573
Fragments arranged by size:
2573 1156 915 639 552 525 16
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6376
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2854 4107 5065
Size: 416 53 83 399 24 1437 1253 958
Cuts at: 5065 442
Size: 1753
Fragments arranged by size:
1753 1437 1253 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6376
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2596 2683 5036 1239
Size: 1357 87 2353 2579
Fragments arranged by size:
2579 2353 1357 87
Bpu10I CC'TnA_GC
Cuts at: 2034 2839 3576 2034
Size: 805 737 4834
Fragments arranged by size:
4834 805 737
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2126 2255 2657 4501 5366 5664 2126
Size: 129 402 1844 865 298 2838
Fragments arranged by size:
2838 1844 865 402 298 129
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5039 882
Size: 617 3540 2219
Fragments arranged by size:
3540 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2275 4167 493
Size: 711 1071 1892 2702
Fragments arranged by size:
2702 1892 1071 711
BsaBI GATnn'nnATC
Cuts at: 6370 6370
Size: 6376
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4183 4565 5200 275
Size: 53 83 186 936 2650 382 635 1451
Fragments arranged by size:
2650 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4809 5207 5481 5628 6039 4809
Size: 398 274 147 411 5146
Fragments arranged by size:
5146 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2681 2711 2771 2801 1178
Size: 30 165 30 1278 30 60 30 4753
Fragments arranged by size:
4753 1278 165 60 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2264 2453 2980 1186
Size: 1078 189 527 4582
Fragments arranged by size:
4582 1078 527 189
BseYI C'CCAG_C
Cuts at: 2053 2609 2613 3076 3273 3662 5579 6075 2053
Size: 556 4 463 197 389 1917 496 2354
Fragments arranged by size:
2354 1917 556 496 463 389 197 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2285 2966 3051 3537 3628 1163
Size: 573 549 681 85 486 91 3911
Fragments arranged by size:
3911 681 573 549 486 91 85
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2430 3826 4587 5615 1540
Size: 275 186 429 1396 761 1028 2301
Fragments arranged by size:
2301 1396 1028 761 429 275 186
BsiHKAI G_wGCw'C
Cuts at: 2342 2840 2867 2883 3290 3327 4437 4522 5593
Size: 498 27 16 407 37 1110 85 1071
Cuts at: 5593 2342
Size: 3125
Fragments arranged by size:
3125 1110 1071 498 407 85 37 27
16
BsmI GAATG_Cn'
Cuts at: 2638 3887 3980 2638
Size: 1249 93 5034
Fragments arranged by size:
5034 1249 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2342 2840 2867 2883 3097 3290
Size: 399 250 302 498 27 16 214 193
Cuts at: 3290 3327 3522 3634 3695 4437 4522 5593 1391
Size: 37 195 112 61 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 498 399 302 250 214
195 193 112 85 61 37 27 16
BspEI T'CCGG_A
Cuts at: 6039 6039
Size: 6376
BspHI T'CATG_A
Cuts at: 4265 5995 4265
Size: 1730 4646
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3024 3580 3731 844
Size: 2180 556 151 3489
Fragments arranged by size:
3489 2180 556 151
BsrBI CCG'CTC
Cuts at: 1394 1823 4263 1394
Size: 429 2440 3507
Fragments arranged by size:
3507 2440 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3427 4871 5045 65
Size: 1183 2179 1444 174 1396
Fragments arranged by size:
2179 1444 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3268 3290 5020 5207 1700
Size: 110 1458 22 1730 187 2869
Fragments arranged by size:
2869 1730 1458 187 110 22
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6376
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6376
BssSI C'ACGA_G
Cuts at: 4436 5448 4436
Size: 1012 5364
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2263 2782 3025 794
Size: 1469 519 243 4145
Fragments arranged by size:
4145 1469 519 243
BstBI TT'CG_AA
Cuts at: 5180 5180
Size: 6376
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6376
BstXI CCAn_nnnn'nTGG
Cuts at: 2053 3238 2053
Size: 1185 5191
BstYI r'GATC_y
Cuts at: 1972 2503 3171 3213 3706 4073 4458 4475 5927
Size: 531 668 42 493 367 385 17 1452
Cuts at: 5927 6013 6025 6371 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 668 531 493 385 367 346
86 42 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2089 3584 513
Size: 552 1024 1495 3305
Fragments arranged by size:
3305 1495 1024 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3169 487
Size: 683 56 1943 3694
Fragments arranged by size:
3694 1943 683 56
BtsI GCAGTG_nn'
Cuts at: 2168 2792 2943 2983 3039 3363 3892 4686 4706
Size: 624 151 40 56 324 529 794 20
Cuts at: 4706 2168
Size: 3838
Fragments arranged by size:
3838 794 624 529 324 151 56 40
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6376
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6341
DraI TTT'AAA
Cuts at: 3680 3680
Size: 6376
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5383 809
Size: 507 4067 1802
Fragments arranged by size:
4067 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2433 2931 3292
Size: 54 1425 50 275 18 603 498 361
Cuts at: 3292 3823 4712 6341 8
Size: 531 889 1629 43
Fragments arranged by size:
1629 1425 889 603 531 498 361 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3823 1537
Size: 275 2011 4090
Fragments arranged by size:
4090 2011 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3162 4306 1353
Size: 1809 1144 3423
Fragments arranged by size:
3423 1809 1144
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2615 4963 5337 5483 387
Size: 1064 138 1026 2348 374 146 1280
Fragments arranged by size:
2348 1280 1064 1026 374 146 138
EcoICRI GAG'CTC
Cuts at: 2838 2838
Size: 6376
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2411 2461 2596 2683 4439 5036 5823
Size: 393 779 50 135 87 1756 597 787
Cuts at: 5823 6091 1239
Size: 268 1524
Fragments arranged by size:
1756 1524 787 779 597 393 268 135
87 50
EcoNI CCTnn'n_nnAGG
Cuts at: 3575 3575
Size: 6376
EcoO109I rG'GnC_Cy
Cuts at: 2058 2959 3022 3639 2058
Size: 901 63 617 4795
Fragments arranged by size:
4795 901 617 63
EcoRI G'AATT_C
Cuts at: 3685 4063 3685
Size: 378 5998
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2731 2731
Size: 6376
FspI TGC'GCA
Cuts at: 4882 4882
Size: 6376
HaeII r_GCGC'y
Cuts at: 1678 5523 1678
Size: 3845 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2429 2461 2681 2713 3492
Size: 32 135 32 857 32 220 32 779
Cuts at: 3492 3524 5017 5049 5091 5123 1373
Size: 32 1493 32 42 32 2626
Fragments arranged by size:
2626 1493 857 779 220 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3720 3966 669
Size: 988 281 1782 246 3079
Fragments arranged by size:
3079 1782 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6376
HpaI GTT'AAC
Cuts at: 3966 3966
Size: 6376
KpnI G_GTAC'C
Cuts at: 3701 3701
Size: 6376
MfeI C'AATT_G
Cuts at: 3975 3975
Size: 6376
MluI A'CGCG_T
Cuts at: 6252 6252
Size: 6376
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1989 5490 5674 1305
Size: 684 3501 184 2007
Fragments arranged by size:
3501 2007 684 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2933 10
Size: 54 1425 343 1101 3453
Fragments arranged by size:
3453 1425 1101 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2186 2429 3236 4334 4693 4852 518
Size: 648 1020 243 807 1098 359 159 2042
Fragments arranged by size:
2042 1098 1020 807 648 359 243 159
MspA1I CmG'CkG
Cuts at: 1606 2569 2613 2652 3277 3792 4469 5617 5862
Size: 963 44 39 625 515 677 1148 245
Cuts at: 5862 1606
Size: 2120
Fragments arranged by size:
2120 1148 963 677 625 515 245 44
39
NaeI GCC'GGC
Cuts at: 3270 3270
Size: 6376
NcoI C'CATG_G
Cuts at: 513 1065 2089 513
Size: 552 1024 4800
Fragments arranged by size:
4800 1024 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6376
NgoMIV G'CCGG_C
Cuts at: 3268 3268
Size: 6376
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6376
NotI GC'GGCC_GC
Cuts at: 3823 3823
Size: 6376
NspI r_CATG'y
Cuts at: 1132 1229 3442 3734 5279 1132
Size: 97 2213 292 1545 2229
Fragments arranged by size:
2229 2213 1545 292 97
PasI CC'CwG_GG
Cuts at: 1348 2771 3626 1348
Size: 1423 855 4098
Fragments arranged by size:
4098 1423 855
PciI A'CATG_T
Cuts at: 5275 5275
Size: 6376
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3083 1108
Size: 1975 4401
PfoI T'CCnGG_A
Cuts at: 2097 2742 2889 2097
Size: 645 147 5584
Fragments arranged by size:
5584 645 147
PmeI GTTT'AAAC
Cuts at: 3680 3680
Size: 6376
PmlI CAC'GTG
Cuts at: 2275 2275
Size: 6376
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4445 4477 5958 5990 4445
Size: 32 1481 32 4831
Fragments arranged by size:
4831 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2959 2959
Size: 6376
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6376
PsiI TTA'TAA
Cuts at: 3946 3946
Size: 6376
PstI C_TGCA'G
Cuts at: 830 3021 3496 3728 830
Size: 2191 475 232 3478
Fragments arranged by size:
3478 2191 475 232
PvuI CG_AT'CG
Cuts at: 2001 2430 2001
Size: 429 5947
PvuII CAG'CTG
Cuts at: 2569 2613 2652 2569
Size: 44 39 6293
Fragments arranged by size:
6293 44 39
SacI G_AGCT'C
Cuts at: 2840 2840
Size: 6376
SalI G'TCGA_C
Cuts at: 1936 3718 1936
Size: 1782 4594
SanDI GG'GwC_CC
Cuts at: 2959 2959
Size: 6376
SbfI CC_TGCA'GG
Cuts at: 3021 3728 3021
Size: 707 5669
ScaI AGT'ACT
Cuts at: 3308 3602 4624 6336 3308
Size: 294 1022 1712 3348
Fragments arranged by size:
3348 1712 1022 294
SfcI C'TryA_G
Cuts at: 826 1045 3017 3492 3724 4859 5540 5731 826
Size: 219 1972 475 232 1135 681 191 1471
Fragments arranged by size:
1972 1471 1135 681 475 232 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6376
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6376
SmaI CCC'GGG
Cuts at: 1872 2222 3703 1872
Size: 350 1481 4545
Fragments arranged by size:
4545 1481 350
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2141 2234 2672 4480 5381 5643
Size: 197 926 198 93 438 1808 901 262
Cuts at: 5643 820
Size: 1553
Fragments arranged by size:
1808 1553 926 901 438 262 198 197
93
SnaBI TAC'GTA
Cuts at: 493 4167 493
Size: 3674 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6376
SphI G_CATG'C
Cuts at: 1132 3734 1132
Size: 2602 3774
SspI AAT'ATT
Cuts at: 5 52 4300 5
Size: 47 4248 2081
Fragments arranged by size:
4248 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6376
StyI C'CwwG_G
Cuts at: 513 1065 2089 2343 2367 3815 513
Size: 552 1024 254 24 1448 3074
Fragments arranged by size:
3074 1448 1024 552 254 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2017 2155 2894 4403 4420 4573 4758
Size: 1255 437 138 739 1509 17 153 185
Cuts at: 4758 5203 325
Size: 445 1498
Fragments arranged by size:
1509 1498 1255 739 445 437 185 153
138 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3306 3600 4622
Size: 274 80 33 51 1443 1329 294 1022
Cuts at: 4622 6334 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1329 1022 294 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2771 2803 6336 6368 2771
Size: 32 3533 32 2779
Fragments arranged by size:
3533 2779 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3506 1316
Size: 2190 4186
XbaI T'CTAG_A
Cuts at: 3712 3712
Size: 6376
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2428 3568 2428
Size: 1140 5236
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6376
XmaI C'CCGG_G
Cuts at: 1870 2220 3701 1870
Size: 350 1481 4545
Fragments arranged by size:
4545 1481 350
XmnI GAAnn'nnTTC
Cuts at: 4505 4505
Size: 6376
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4184 276
Size: 53 83 186 936 2650 2468
Fragments arranged by size:
2650 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PspOMI PspXI
PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI
SwaI