Restriction map for
pFN21AE6430
(Circular) (Six-base) MAPSORT of: pFN21AE6430.seq Check: 6345 from: 1 to: 6547
pFN21AE6430 6547 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4357 278
Size: 53 83 186 936 2821 2468
Fragments arranged by size:
2821 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3890 1937
Size: 1953 4594
Acc65I G'GTAC_C
Cuts at: 3868 3868
Size: 6547
AclI AA'CG_TT
Cuts at: 1563 3846 4674 5047 1563
Size: 2283 828 373 3063
Fragments arranged by size:
3063 2283 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3101 4610 5994 6262 1632
Size: 1469 1509 1384 268 1917
Fragments arranged by size:
1917 1509 1469 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6350
AflIII A'CryG_T
Cuts at: 1203 3050 5446 6423 1203
Size: 1847 2396 977 1327
Fragments arranged by size:
2396 1847 1327 977
AgeI A'CCGG_T
Cuts at: 5378 5378
Size: 6547
AhdI GACnn_n'nnGTC
Cuts at: 5276 5276
Size: 6547
AleI CACnn'nnGTG
Cuts at: 1166 3635 1166
Size: 2469 4078
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6547
AlwNI CAG_nnn'CTG
Cuts at: 3124 4290 5862 3124
Size: 1166 1572 3809
Fragments arranged by size:
3809 1572 1166
ApaLI G'TGCA_C
Cuts at: 4604 5760 4604
Size: 1156 5391
ApoI r'AATT_y
Cuts at: 1493 2999 3856 4082 4234 6343 1493
Size: 1506 857 226 152 2109 1697
Fragments arranged by size:
2109 1697 1506 857 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5101 160
Size: 872 4069 1606
Fragments arranged by size:
4069 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6547
AvaI C'yCGr_G
Cuts at: 1870 1943 3401 3759 3872 1870
Size: 73 1458 358 113 4545
Fragments arranged by size:
4545 1458 358 113 73
BamHI G'GATC_C
Cuts at: 3418 3877 4244 3418
Size: 459 367 5721
Fragments arranged by size:
5721 459 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3515 3868 5323 618
Size: 325 217 627 1728 353 1455 1842
Fragments arranged by size:
1842 1728 1455 627 353 325 217
BanII G_rGCy'C
Cuts at: 1391 3183 3866 1391
Size: 1792 683 4072
Fragments arranged by size:
4072 1792 683
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5576
BbvCI CC'TCA_GC
Cuts at: 2316 2721 2316
Size: 405 6142
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3260 3294 4738 4772 1903
Size: 34 1323 34 1444 34 3678
Fragments arranged by size:
3678 1444 1323 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4439 5649 4439
Size: 1210 5337
BclI T'GATC_A
Cuts at: 1548 2877 1548
Size: 1329 5218
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5158 136
Size: 107 122 71 631 605 3486 1525
Fragments arranged by size:
3486 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6542 6542
Size: 6547
BlpI GC'TnA_GC
Cuts at: 2325 3964 2325
Size: 1639 4908
Bme1580I G_kGCm'C
Cuts at: 1790 4608 5764 1790
Size: 2818 1156 2573
Fragments arranged by size:
2818 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2713 1318
Size: 1395 5152
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4278 5236 442
Size: 416 53 83 399 24 2861 958 1753
Fragments arranged by size:
2861 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6547
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2210 2750 3146 3197 3857 5207 1239
Size: 971 540 396 51 660 1350 2579
Fragments arranged by size:
2579 1350 971 660 540 396 51
Bpu10I CC'TnA_GC
Cuts at: 2316 2568 2721 2316
Size: 252 153 6142
Fragments arranged by size:
6142 252 153
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3631 4672 5537 5835 3631
Size: 1041 865 298 4343
Fragments arranged by size:
4343 1041 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2156 3105 5210 882
Size: 617 657 949 2105 2219
Fragments arranged by size:
2219 2105 949 657 617
BsaAI yAC'GTr
Cuts at: 493 1204 4338 493
Size: 711 3134 2702
Fragments arranged by size:
3134 2702 711
BsaBI GATnn'nnATC
Cuts at: 6541 6541
Size: 6547
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4354 4736 5371 275
Size: 53 83 186 936 2821 382 635 1451
Fragments arranged by size:
2821 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4980 5378 5652 5799 6210 4980
Size: 398 274 147 411 5317
Fragments arranged by size:
5317 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2189 2219 2816 2846 1178
Size: 30 165 30 786 30 597 30 4879
Fragments arranged by size:
4879 786 597 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2207 1186
Size: 1021 5526
BseYI C'CCAG_C
Cuts at: 2510 2793 5750 6246 2510
Size: 283 2957 496 2811
Fragments arranged by size:
2957 2811 496 283
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2962 1163
Size: 573 1226 4748
Fragments arranged by size:
4748 1226 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3997 4758 5786 1540
Size: 275 186 1996 761 1028 2301
Fragments arranged by size:
2301 1996 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3183 3402 4608 4693 5764 3183
Size: 219 1206 85 1071 3966
Fragments arranged by size:
3966 1206 1071 219 85
BsmI GAATG_Cn'
Cuts at: 2401 3211 3224 3558 4058 4151 2401
Size: 810 13 334 500 93 4797
Fragments arranged by size:
4797 810 500 334 93 13
BsmBI CGTCTCn'nnnn_
Cuts at: 2472 2914 2985 3456 2472
Size: 442 71 471 5563
Fragments arranged by size:
5563 471 442 71
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3183 3402 3866 4608 4693 5764 1391
Size: 399 1393 219 464 742 85 1071 2174
Fragments arranged by size:
2174 1393 1071 742 464 399 219 85
BspEI T'CCGG_A
Cuts at: 6210 6210
Size: 6547
BspHI T'CATG_A
Cuts at: 4436 6166 4436
Size: 1730 4817
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3486 3902 844
Size: 2642 416 3489
Fragments arranged by size:
3489 2642 416
BsrBI CCG'CTC
Cuts at: 1394 1823 4434 1394
Size: 429 2611 3507
Fragments arranged by size:
3507 2611 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5042 5216 65
Size: 1183 3794 174 1396
Fragments arranged by size:
3794 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5191 5378 1700
Size: 110 3381 187 2869
Fragments arranged by size:
3381 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3053 96
Size: 2957 3590
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6547
BssSI C'ACGA_G
Cuts at: 4607 5619 4607
Size: 1012 5535
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6547
BstBI TT'CG_AA
Cuts at: 5351 5351
Size: 6547
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6547
BstYI r'GATC_y
Cuts at: 1972 2761 3418 3577 3877 4244 4629 4646 6098
Size: 789 657 159 300 367 385 17 1452
Cuts at: 6098 6184 6196 6542 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 789 657 385 367 346 300
159 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3687 513
Size: 552 2622 3373
Fragments arranged by size:
3373 2622 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5808
Fragments arranged by size:
5808 683 56
BtsI GCAGTG_nn'
Cuts at: 3490 4063 4857 4877 3490
Size: 573 794 20 5160
Fragments arranged by size:
5160 794 573 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6547
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6512
DraI TTT'AAA
Cuts at: 3851 3851
Size: 6547
DraIII CAC_nnn'GTG
Cuts at: 2077 2966 2077
Size: 889 5658
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5554 809
Size: 507 4238 1802
Fragments arranged by size:
4238 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3253 3994 4883
Size: 54 1425 50 275 18 1423 741 889
Cuts at: 4883 6512 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1423 889 741 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3994 1537
Size: 275 2182 4090
Fragments arranged by size:
4090 2182 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4477 1353
Size: 3124 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2294 3391 5134 5508 5654 387
Size: 1064 138 705 1097 1743 374 146 1280
Fragments arranged by size:
1743 1280 1097 1064 705 374 146 138
EcoICRI GAG'CTC
Cuts at: 3181 3181
Size: 6547
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2210 2750 3101 3146 3197 3857 4610
Size: 393 578 540 351 45 51 660 753
Cuts at: 4610 5207 5994 6262 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 753 660 597 578 540 393
351 268 51 45
EcoO109I rG'GnC_Cy
Cuts at: 3412 3498 3541 3811 3831 3412
Size: 86 43 270 20 6128
Fragments arranged by size:
6128 270 86 43 20
EcoRI G'AATT_C
Cuts at: 2999 3856 4234 2999
Size: 857 378 5312
Fragments arranged by size:
5312 857 378
EcoRV GAT'ATC
Cuts at: 2674 2674
Size: 6547
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2851 2851
Size: 6547
FspI TGC'GCA
Cuts at: 3236 5053 3236
Size: 1817 4730
HaeII r_GCGC'y
Cuts at: 1678 5694 1678
Size: 4016 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3410 3442 3716 3748 5188
Size: 32 135 32 1838 32 274 32 1440
Cuts at: 5188 5220 5262 5294 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1838 1440 274 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3429 3891 4137 669
Size: 988 281 1491 462 246 3079
Fragments arranged by size:
3079 1491 988 462 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6547
HpaI GTT'AAC
Cuts at: 4137 4137
Size: 6547
KpnI G_GTAC'C
Cuts at: 3872 3872
Size: 6547
MfeI C'AATT_G
Cuts at: 4146 4146
Size: 6547
MluI A'CGCG_T
Cuts at: 6423 6423
Size: 6547
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2086 5661 5845 1305
Size: 781 3575 184 2007
Fragments arranged by size:
3575 2007 781 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3255 10
Size: 54 1425 343 1423 3302
Fragments arranged by size:
3302 1425 1423 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3635 4505 4864 5023 518
Size: 648 2469 870 359 159 2042
Fragments arranged by size:
2469 2042 870 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3963 4640 5788 6033 1606
Size: 2357 677 1148 245 2120
Fragments arranged by size:
2357 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3687 513
Size: 552 2622 3373
Fragments arranged by size:
3373 2622 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6547
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6547
NotI GC'GGCC_GC
Cuts at: 3994 3994
Size: 6547
NspI r_CATG'y
Cuts at: 1132 1229 3054 3905 5450 1132
Size: 97 1825 851 1545 2229
Fragments arranged by size:
2229 1825 1545 851 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6547
PciI A'CATG_T
Cuts at: 3050 5446 3050
Size: 2396 4151
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2623 3804 1108
Size: 1515 1181 3851
Fragments arranged by size:
3851 1515 1181
PfoI T'CCnGG_A
Cuts at: 3414 3500 3414
Size: 86 6461
PmeI GTTT'AAAC
Cuts at: 3851 3851
Size: 6547
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4616 4648 6129 6161 4616
Size: 32 1481 32 5002
Fragments arranged by size:
5002 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3412 3498 3541 3811 3831 3412
Size: 86 43 270 20 6128
Fragments arranged by size:
6128 270 86 43 20
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6547
PsiI TTA'TAA
Cuts at: 4117 4117
Size: 6547
PspXI vC'TCGA_Gb
Cuts at: 3401 3401
Size: 6547
PstI C_TGCA'G
Cuts at: 830 2917 3497 3550 3899 830
Size: 2087 580 53 349 3478
Fragments arranged by size:
3478 2087 580 349 53
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6547
SacI G_AGCT'C
Cuts at: 3183 3183
Size: 6547
SalI G'TCGA_C
Cuts at: 1936 3889 1936
Size: 1953 4594
SanDI GG'GwC_CC
Cuts at: 3541 3541
Size: 6547
SbfI CC_TGCA'GG
Cuts at: 3550 3899 3550
Size: 349 6198
ScaI AGT'ACT
Cuts at: 2834 4795 6507 2834
Size: 1961 1712 2874
Fragments arranged by size:
2874 1961 1712
SexAI A'CCwGG_T
Cuts at: 2616 2616
Size: 6547
SfcI C'TryA_G
Cuts at: 826 1045 2913 3493 3546 3895 5030 5711 5902
Size: 219 1868 580 53 349 1135 681 191
Cuts at: 5902 826
Size: 1471
Fragments arranged by size:
1868 1471 1135 681 580 349 219 191
53
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6547
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6547
SmaI CCC'GGG
Cuts at: 1872 3761 3874 1872
Size: 1889 113 4545
Fragments arranged by size:
4545 1889 113
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3401 3610 4651 5552 5814 820
Size: 197 926 1458 209 1041 901 262 1553
Fragments arranged by size:
1553 1458 1041 926 901 262 209 197
SnaBI TAC'GTA
Cuts at: 493 4338 493
Size: 3845 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6547
SphI G_CATG'C
Cuts at: 1132 3905 1132
Size: 2773 3774
SspI AAT'ATT
Cuts at: 5 52 2442 2464 4471 5
Size: 47 2390 22 2007 2081
Fragments arranged by size:
2390 2081 2007 47 22
StuI AGG'CCT
Cuts at: 1517 2490 1517
Size: 973 5574
StyI C'CwwG_G
Cuts at: 513 1065 3348 3687 3986 513
Size: 552 2283 339 299 3074
Fragments arranged by size:
3074 2283 552 339 299
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4574 4591 4744 4929 5374 325
Size: 1255 2994 17 153 185 445 1498
Fragments arranged by size:
2994 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2832 2899 3053
Size: 274 80 33 51 1443 855 67 154
Cuts at: 3053 4793 6505 96
Size: 1740 1712 138
Fragments arranged by size:
1740 1712 1443 855 274 154 138 80
67 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2121 2153 2753 2785 3482 3514 6507 6539 2121
Size: 32 600 32 697 32 2993 32 2129
Fragments arranged by size:
2993 2129 697 600 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2243 2609 2711 1316
Size: 927 366 102 5152
Fragments arranged by size:
5152 927 366 102
XbaI T'CTAG_A
Cuts at: 3367 3883 3367
Size: 516 6031
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3685 3685
Size: 6547
XhoI C'TCGA_G
Cuts at: 1943 3401 1943
Size: 1458 5089
XmaI C'CCGG_G
Cuts at: 1870 3759 3872 1870
Size: 1889 113 4545
Fragments arranged by size:
4545 1889 113
XmnI GAAnn'nnTTC
Cuts at: 3645 4676 3645
Size: 1031 5516
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4355 276
Size: 53 83 186 936 2821 2468
Fragments arranged by size:
2821 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspXI PstI PvuI SacI SalI
SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BstXI BstZ17I Bsu36I EcoNI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PsrI PvuII RsrII SacII SapI SfiI
SfoI SrfI SwaI