Restriction map for
pFN21AE6428
(Circular) (Six-base) MAPSORT of: pFN21AE6428.seq Check: 7839 from: 1 to: 6085
pFN21AE6428 6085 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3895 278
Size: 53 83 186 936 2359 2468
Fragments arranged by size:
2468 2359 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2580 3428 1937
Size: 643 848 4594
Fragments arranged by size:
4594 848 643
Acc65I G'GTAC_C
Cuts at: 3406 3406
Size: 6085
AclI AA'CG_TT
Cuts at: 1563 4212 4585 1563
Size: 2649 373 3063
Fragments arranged by size:
3063 2649 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2074 2256 2981 4148 5532 5800 1632
Size: 442 182 725 1167 1384 268 1917
Fragments arranged by size:
1917 1384 1167 725 442 268 182
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5888
AflIII A'CryG_T
Cuts at: 1203 4984 5961 1203
Size: 3781 977 1327
Fragments arranged by size:
3781 1327 977
AgeI A'CCGG_T
Cuts at: 4916 4916
Size: 6085
AhdI GACnn_n'nnGTC
Cuts at: 2673 4814 2673
Size: 2141 3944
AleI CACnn'nnGTG
Cuts at: 1166 2340 1166
Size: 1174 4911
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3145 1184
Size: 1961 4124
AlwNI CAG_nnn'CTG
Cuts at: 2606 3828 5400 2606
Size: 1222 1572 3291
Fragments arranged by size:
3291 1572 1222
ApaI G_GGCC'C
Cuts at: 2889 2889
Size: 6085
ApaLI G'TGCA_C
Cuts at: 4142 5298 4142
Size: 1156 4929
ApoI r'AATT_y
Cuts at: 1493 2291 3394 3620 3772 5881 1493
Size: 798 1103 226 152 2109 1697
Fragments arranged by size:
2109 1697 1103 798 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2421 4639 160
Size: 872 1389 2218 1606
Fragments arranged by size:
2218 1606 1389 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6085
AvaI C'yCGr_G
Cuts at: 1870 1943 2011 3410 1870
Size: 73 68 1399 4545
Fragments arranged by size:
4545 1399 73 68
BamHI G'GATC_C
Cuts at: 3415 3782 3415
Size: 367 5718
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3406 4861 618
Size: 325 217 627 1619 1455 1842
Fragments arranged by size:
1842 1619 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2034 2889 3404 1391
Size: 643 855 515 4072
Fragments arranged by size:
4072 855 643 515
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5114
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3154 3188 4276 4310 1903
Size: 34 1217 34 1088 34 3678
Fragments arranged by size:
3678 1217 1088 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3977 5187 3977
Size: 1210 4875
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6085
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4696 136
Size: 107 122 71 631 605 3024 1525
Fragments arranged by size:
3024 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2241 6080 2241
Size: 3839 2246
BlpI GC'TnA_GC
Cuts at: 2105 3502 2105
Size: 1397 4688
Bme1580I G_kGCm'C
Cuts at: 1790 2889 4146 5302 1790
Size: 1099 1257 1156 2573
Fragments arranged by size:
2573 1257 1156 1099
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6085
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3816 4774 442
Size: 416 53 83 399 24 2399 958 1753
Fragments arranged by size:
2399 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2087 3146 1056
Size: 1031 1059 3995
Fragments arranged by size:
3995 1059 1031
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2132 2180 3222 4745 1239
Size: 893 48 1042 1523 2579
Fragments arranged by size:
2579 1523 1042 893 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2703 4210 5075 5373 2703
Size: 1507 865 298 3415
Fragments arranged by size:
3415 1507 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4748 882
Size: 617 3249 2219
Fragments arranged by size:
3249 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3876 493
Size: 711 2672 2702
Fragments arranged by size:
2702 2672 711
BsaBI GATnn'nnATC
Cuts at: 6079 6079
Size: 6085
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2190 3892 4274 4909
Size: 53 83 186 936 657 1702 382 635
Cuts at: 4909 275
Size: 1451
Fragments arranged by size:
1702 1451 936 657 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4518 4916 5190 5337 5748 4518
Size: 398 274 147 411 4855
Fragments arranged by size:
4855 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3045 3075 1178
Size: 30 165 30 1642 30 4188
Fragments arranged by size:
4188 1642 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2076 2426 3047 1186
Size: 890 350 621 4224
Fragments arranged by size:
4224 890 621 350
BseYI C'CCAG_C
Cuts at: 5288 5784 5288
Size: 496 5589
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2195 2965 1163
Size: 573 459 770 4283
Fragments arranged by size:
4283 770 573 459
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2049 3535 4296 5324 1540
Size: 275 186 48 1486 761 1028 2301
Fragments arranged by size:
2301 1486 1028 761 275 186 48
BsiHKAI G_wGCw'C
Cuts at: 3089 4146 4231 5302 3089
Size: 1057 85 1071 3872
Fragments arranged by size:
3872 1071 1057 85
BsmI GAATG_Cn'
Cuts at: 2652 3319 3596 3689 2652
Size: 667 277 93 5048
Fragments arranged by size:
5048 667 277 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2889 3089 3404 4146 4231 5302
Size: 399 244 855 200 315 742 85 1071
Cuts at: 5302 1391
Size: 2174
Fragments arranged by size:
2174 1071 855 742 399 315 244 200
85
BspEI T'CCGG_A
Cuts at: 5748 5748
Size: 6085
BspHI T'CATG_A
Cuts at: 3303 3974 5704 3303
Size: 671 1730 3684
Fragments arranged by size:
3684 1730 671
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2758 3440 844
Size: 1914 682 3489
Fragments arranged by size:
3489 1914 682
BsrBI CCG'CTC
Cuts at: 1394 1823 3972 1394
Size: 429 2149 3507
Fragments arranged by size:
3507 2149 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4580 4754 65
Size: 1183 3332 174 1396
Fragments arranged by size:
3332 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2051 4729 4916 1700
Size: 110 241 2678 187 2869
Fragments arranged by size:
2869 2678 241 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6085
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6085
BssSI C'ACGA_G
Cuts at: 3044 4145 5157 3044
Size: 1101 1012 3972
Fragments arranged by size:
3972 1101 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2750 2783 794
Size: 1956 33 4096
Fragments arranged by size:
4096 1956 33
BstBI TT'CG_AA
Cuts at: 4889 4889
Size: 6085
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6085
BstYI r'GATC_y
Cuts at: 1972 2241 3415 3782 4167 4184 5636 5722 5734
Size: 269 1174 367 385 17 1452 86 12
Cuts at: 5734 6080 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1174 385 367 346 269 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4594
Fragments arranged by size:
4594 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2949 487
Size: 683 56 1723 3623
Fragments arranged by size:
3623 1723 683 56
BtsI GCAGTG_nn'
Cuts at: 3601 4395 4415 3601
Size: 794 20 5271
Fragments arranged by size:
5271 794 20
ClaI AT'CG_AT
Cuts at: 1554 2955 1554
Size: 1401 4684
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6050
DraI TTT'AAA
Cuts at: 3389 3389
Size: 6085
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5092 809
Size: 507 3776 1802
Fragments arranged by size:
3776 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2046 2203
Size: 54 1425 50 275 18 195 21 157
Cuts at: 2203 3532 4421 6050 8
Size: 1329 889 1629 43
Fragments arranged by size:
1629 1425 1329 889 275 195 157 54
50 43 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 2046 3532 1537
Size: 275 234 1486 4090
Fragments arranged by size:
4090 1486 275 234
EarI CTCTTCn'nnn_
Cuts at: 1353 2232 4015 1353
Size: 879 1783 3423
Fragments arranged by size:
3423 1783 879
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2084 4672 5046 5192 387
Size: 1064 138 495 2588 374 146 1280
Fragments arranged by size:
2588 1280 1064 495 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2074 2132 2180 2256 2981 3222 4148
Size: 393 442 58 48 76 725 241 926
Cuts at: 4148 4745 5532 5800 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 926 787 725 597 442 393 268
241 76 58 48
EcoNI CCTnn'n_nnAGG
Cuts at: 3029 3029
Size: 6085
EcoO109I rG'GnC_Cy
Cuts at: 2885 2885
Size: 6085
EcoRI G'AATT_C
Cuts at: 3394 3772 3394
Size: 378 5707
FspI TGC'GCA
Cuts at: 4591 4591
Size: 6085
HaeII r_GCGC'y
Cuts at: 1678 5232 1678
Size: 3554 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2407 2437 2439 2469 2659
Size: 32 135 32 835 30 2 30 190
Cuts at: 2659 2691 3073 3105 4726 4758 4800 4832 1373
Size: 32 382 32 1621 32 42 32 2626
Fragments arranged by size:
2626 1621 835 382 190 135 42 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2581 3327 3429 3675 669
Size: 988 281 643 746 102 246 3079
Fragments arranged by size:
3079 988 746 643 281 246 102
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6085
HpaI GTT'AAC
Cuts at: 3327 3675 3327
Size: 348 5737
KpnI G_GTAC'C
Cuts at: 3410 3410
Size: 6085
MfeI C'AATT_G
Cuts at: 2324 3684 2324
Size: 1360 4725
MluI A'CGCG_T
Cuts at: 5961 5961
Size: 6085
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2566 5199 5383 1305
Size: 1261 2633 184 2007
Fragments arranged by size:
2633 2007 1261 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2205 10
Size: 54 1425 343 373 3890
Fragments arranged by size:
3890 1425 373 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2340 3158 4043 4402 4561 518
Size: 648 1174 818 885 359 159 2042
Fragments arranged by size:
2042 1174 885 818 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2209 3501 4178 5326 5571 1606
Size: 603 1292 677 1148 245 2120
Fragments arranged by size:
2120 1292 1148 677 603 245
NaeI GCC'GGC
Cuts at: 2053 2053
Size: 6085
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4594
Fragments arranged by size:
4594 939 552
NdeI CA'TA_TG
Cuts at: 387 2671 387
Size: 2284 3801
NgoMIV G'CCGG_C
Cuts at: 2051 2051
Size: 6085
NheI G'CTAG_C
Cuts at: 1052 2083 3142 1052
Size: 1031 1059 3995
Fragments arranged by size:
3995 1059 1031
NotI GC'GGCC_GC
Cuts at: 2046 3532 2046
Size: 1486 4599
NspI r_CATG'y
Cuts at: 1132 1229 2772 3126 3211 3443 4988 1132
Size: 97 1543 354 85 232 1545 2229
Fragments arranged by size:
2229 1545 1543 354 232 97 85
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6085
PciI A'CATG_T
Cuts at: 4984 4984
Size: 6085
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6085
PfoI T'CCnGG_A
Cuts at: 2193 2193
Size: 6085
PmeI GTTT'AAAC
Cuts at: 3389 3389
Size: 6085
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4154 4186 5667 5699 4154
Size: 32 1481 32 4540
Fragments arranged by size:
4540 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6085
PsiI TTA'TAA
Cuts at: 3655 3655
Size: 6085
PspOMI G'GGCC_C
Cuts at: 2885 2885
Size: 6085
PstI C_TGCA'G
Cuts at: 830 3437 830
Size: 2607 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6085
PvuII CAG'CTG
Cuts at: 2209 2209
Size: 6085
SalI G'TCGA_C
Cuts at: 1936 2579 3427 1936
Size: 643 848 4594
Fragments arranged by size:
4594 848 643
SbfI CC_TGCA'GG
Cuts at: 3437 3437
Size: 6085
ScaI AGT'ACT
Cuts at: 3010 4333 6045 3010
Size: 1323 1712 3050
Fragments arranged by size:
3050 1712 1323
SfcI C'TryA_G
Cuts at: 826 1045 2840 3433 4568 5249 5440 826
Size: 219 1795 593 1135 681 191 1471
Fragments arranged by size:
1795 1471 1135 681 593 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6085
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6085
SmaI CCC'GGG
Cuts at: 1872 2013 3412 1872
Size: 141 1399 4545
Fragments arranged by size:
4545 1399 141
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2718 4189 5090 5352 820
Size: 197 926 775 1471 901 262 1553
Fragments arranged by size:
1553 1471 926 901 775 262 197
SnaBI TAC'GTA
Cuts at: 493 3876 493
Size: 3383 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6085
SphI G_CATG'C
Cuts at: 1132 3443 1132
Size: 2311 3774
SspI AAT'ATT
Cuts at: 5 52 3184 4009 5
Size: 47 3132 825 2081
Fragments arranged by size:
3132 2081 825 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6085
StyI C'CwwG_G
Cuts at: 513 1065 2004 3524 513
Size: 552 939 1520 3074
Fragments arranged by size:
3074 1520 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2352 4112 4129 4282 4467 4912 325
Size: 1255 772 1760 17 153 185 445 1498
Fragments arranged by size:
1760 1498 1255 772 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3008 4331 6043
Size: 274 80 33 51 1443 1031 1323 1712
Cuts at: 6043 96
Size: 138
Fragments arranged by size:
1712 1443 1323 1031 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3045 3077 6045 6077 3045
Size: 32 2968 32 3053
Fragments arranged by size:
3053 2968 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2373 1316
Size: 1057 5028
XbaI T'CTAG_A
Cuts at: 3421 3421
Size: 6085
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6085
XmaI C'CCGG_G
Cuts at: 1870 2011 3410 1870
Size: 141 1399 4545
Fragments arranged by size:
4545 1399 141
XmnI GAAnn'nnTTC
Cuts at: 4214 4214
Size: 6085
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3893 276
Size: 53 83 186 936 2359 2468
Fragments arranged by size:
2468 2359 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI