Restriction map for
pFN21AE6427
(Circular) (Six-base) MAPSORT of: pFN21AE6427.seq Check: 9777 from: 1 to: 6568
pFN21AE6427 6568 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4378 278
Size: 53 83 186 936 2842 2468
Fragments arranged by size:
2842 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3911 1937
Size: 1974 4594
Acc65I G'GTAC_C
Cuts at: 3889 3889
Size: 6568
AclI AA'CG_TT
Cuts at: 1563 4695 5068 1563
Size: 3132 373 3063
Fragments arranged by size:
3132 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2262 3614 3730 4631 6015 6283 1632
Size: 630 1352 116 901 1384 268 1917
Fragments arranged by size:
1917 1384 1352 901 630 268 116
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6371
AflIII A'CryG_T
Cuts at: 1203 5467 6444 1203
Size: 4264 977 1327
Fragments arranged by size:
4264 1327 977
AgeI A'CCGG_T
Cuts at: 5399 5399
Size: 6568
AhdI GACnn_n'nnGTC
Cuts at: 5297 5297
Size: 6568
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6568
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2554 1184
Size: 1370 5198
AlwNI CAG_nnn'CTG
Cuts at: 4311 5883 4311
Size: 1572 4996
ApaLI G'TGCA_C
Cuts at: 3473 4625 5781 3473
Size: 1152 1156 4260
Fragments arranged by size:
4260 1156 1152
ApoI r'AATT_y
Cuts at: 1493 2231 3017 3038 3877 4103 4255 6364 1493
Size: 738 786 21 839 226 152 2109 1697
Fragments arranged by size:
2109 1697 839 786 738 226 152 21
AseI AT'TA_AT
Cuts at: 160 1032 5122 160
Size: 872 4090 1606
Fragments arranged by size:
4090 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6568
AvaI C'yCGr_G
Cuts at: 1870 1943 3206 3893 1870
Size: 73 1263 687 4545
Fragments arranged by size:
4545 1263 687 73
AvrII C'CTAG_G
Cuts at: 2137 2314 2137
Size: 177 6391
BamHI G'GATC_C
Cuts at: 3357 3898 4265 3357
Size: 541 367 5660
Fragments arranged by size:
5660 541 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3889 5344 618
Size: 325 217 627 2102 1455 1842
Fragments arranged by size:
2102 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2022 2807 3678 3887 1391
Size: 631 785 871 209 4072
Fragments arranged by size:
4072 871 785 631 209
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2610 928
Size: 971 711 4886
Fragments arranged by size:
4886 971 711
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4759 4793 1903
Size: 34 2822 34 3678
Fragments arranged by size:
3678 2822 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3391 4460 5670 3391
Size: 1069 1210 4289
Fragments arranged by size:
4289 1210 1069
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6568
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5179 136
Size: 107 122 71 631 605 3507 1525
Fragments arranged by size:
3507 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6563 6563
Size: 6568
BlpI GC'TnA_GC
Cuts at: 3985 3985
Size: 6568
Bme1580I G_kGCm'C
Cuts at: 1790 3477 3847 4629 5785 1790
Size: 1687 370 782 1156 2573
Fragments arranged by size:
2573 1687 1156 782 370
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6568
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4299 5257 442
Size: 416 53 83 399 24 2882 958 1753
Fragments arranged by size:
2882 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6568
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2269 2269
Size: 6568
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3759 5228 1239
Size: 2520 1469 2579
Fragments arranged by size:
2579 2520 1469
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2438 2933 3842 4693 5558 5856 2438
Size: 495 909 851 865 298 3150
Fragments arranged by size:
3150 909 865 851 495 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2074 5231 882
Size: 617 575 3157 2219
Fragments arranged by size:
3157 2219 617 575
BsaAI yAC'GTr
Cuts at: 493 1204 4359 493
Size: 711 3155 2702
Fragments arranged by size:
3155 2702 711
BsaBI GATnn'nnATC
Cuts at: 3308 6562 3308
Size: 3254 3314
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2513 4375 4757 5392
Size: 53 83 186 936 980 1862 382 635
Cuts at: 5392 275
Size: 1451
Fragments arranged by size:
1862 1451 980 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2580 3300 5001 5399 5673 5820 6231 2580
Size: 720 1701 398 274 147 411 2917
Fragments arranged by size:
2917 1701 720 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3537 3567 1178
Size: 30 165 30 2134 30 4179
Fragments arranged by size:
4179 2134 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2495 3260 3524 3593 1186
Size: 1309 765 264 69 4161
Fragments arranged by size:
4161 1309 765 264 69
BseYI C'CCAG_C
Cuts at: 2939 3810 5771 6267 2939
Size: 871 1961 496 3240
Fragments arranged by size:
3240 1961 871 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2968 1163
Size: 573 1232 4763
Fragments arranged by size:
4763 1232 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2914 3340 3532 3639 4018 4779
Size: 275 186 913 426 192 107 379 761
Cuts at: 4779 5807 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 913 761 426 379 275 192
186 107
BsiHKAI G_wGCw'C
Cuts at: 2526 3389 3477 4629 4714 5785 2526
Size: 863 88 1152 85 1071 3309
Fragments arranged by size:
3309 1152 1071 863 88 85
BsiWI C'GTAC_G
Cuts at: 3237 3237
Size: 6568
BsmI GAATG_Cn'
Cuts at: 2735 2796 4079 4172 2735
Size: 61 1283 93 5131
Fragments arranged by size:
5131 1283 93 61
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2526 2807 3389 3477 3678 3847
Size: 399 232 504 281 582 88 201 169
Cuts at: 3847 3887 4629 4714 5785 1391
Size: 40 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 582 504 399 281 232
201 169 88 85 40
BspEI T'CCGG_A
Cuts at: 2580 3300 6231 2580
Size: 720 2931 2917
Fragments arranged by size:
2931 2917 720
BspHI T'CATG_A
Cuts at: 4457 6187 4457
Size: 1730 4838
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3923 844
Size: 3079 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2010 3660 4455 1394
Size: 429 187 1650 795 3507
Fragments arranged by size:
3507 1650 795 429 187
BsrDI GCAATG_nn'
Cuts at: 65 1248 2526 5063 5237 65
Size: 1183 1278 2537 174 1396
Fragments arranged by size:
2537 1396 1278 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2082 2913 3335 5212 5399 1700
Size: 110 272 831 422 1877 187 2869
Fragments arranged by size:
2869 1877 831 422 272 187 110
BsrGI T'GTAC_A
Cuts at: 96 3306 96
Size: 3210 3358
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6568
BssSI C'ACGA_G
Cuts at: 2046 3692 4628 5640 2046
Size: 1646 936 1012 2974
Fragments arranged by size:
2974 1646 1012 936
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6568
BstBI TT'CG_AA
Cuts at: 5372 5372
Size: 6568
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6568
BstXI CCAn_nnnn'nTGG
Cuts at: 2447 2447
Size: 6568
BstYI r'GATC_y
Cuts at: 1972 3201 3357 3816 3898 4265 4650 4667 6119
Size: 1229 156 459 82 367 385 17 1452
Cuts at: 6119 6205 6217 6563 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1229 459 385 367 346 156
86 82 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2034 2223 3144 3463 3537 513
Size: 552 969 189 921 319 74 3544
Fragments arranged by size:
3544 969 921 552 319 189 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2580 3295 487
Size: 683 56 1354 715 3760
Fragments arranged by size:
3760 1354 715 683 56
BtsI GCAGTG_nn'
Cuts at: 3185 3545 4084 4878 4898 3185
Size: 360 539 794 20 4855
Fragments arranged by size:
4855 794 539 360 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6568
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6533
DraI TTT'AAA
Cuts at: 3872 3872
Size: 6568
DraIII CAC_nnn'GTG
Cuts at: 2600 2600
Size: 6568
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5575 809
Size: 507 4259 1802
Fragments arranged by size:
4259 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2661 2889 2911
Size: 54 1425 50 275 18 831 228 22
Cuts at: 2911 3337 3406 3529 3603 3636 3705 4015 4904
Size: 426 69 123 74 33 69 310 889
Cuts at: 4904 6533 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 831 426 310 275 228
123 74 69 69 54 50 43 33
22 18
EagI C'GGCC_G
Cuts at: 1537 1812 2911 3337 3529 3636 4015 1537
Size: 275 1099 426 192 107 379 4090
Fragments arranged by size:
4090 1099 426 379 275 192 107
EarI CTCTTCn'nnn_
Cuts at: 1353 2027 2238 2303 3749 4498 1353
Size: 674 211 65 1446 749 3423
Fragments arranged by size:
3423 1446 749 674 211 65
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5155 5529 5675 387
Size: 1064 138 3566 374 146 1280
Fragments arranged by size:
3566 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2262 3614 3730 3759 4631 5228 6015
Size: 393 630 1352 116 29 872 597 787
Cuts at: 6015 6283 1239
Size: 268 1524
Fragments arranged by size:
1524 1352 872 787 630 597 393 268
116 29
EcoNI CCTnn'n_nnAGG
Cuts at: 2864 2986 2864
Size: 122 6446
EcoO109I rG'GnC_Cy
Cuts at: 2219 2219
Size: 6568
EcoRI G'AATT_C
Cuts at: 3877 4255 3877
Size: 378 6190
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2210 2210
Size: 6568
FspI TGC'GCA
Cuts at: 2030 2096 5074 2030
Size: 66 2978 3524
Fragments arranged by size:
3524 2978 66
HaeII r_GCGC'y
Cuts at: 1678 3197 5715 1678
Size: 1519 2518 2531
Fragments arranged by size:
2531 2518 1519
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2558 2590 3822 3854 5209
Size: 32 135 32 986 32 1232 32 1355
Cuts at: 5209 5241 5283 5315 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1355 1232 986 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3912 4158 669
Size: 988 281 1974 246 3079
Fragments arranged by size:
3079 1974 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3726 748
Size: 2978 3590
HpaI GTT'AAC
Cuts at: 4158 4158
Size: 6568
KpnI G_GTAC'C
Cuts at: 3893 3893
Size: 6568
MfeI C'AATT_G
Cuts at: 4167 4167
Size: 6568
MluI A'CGCG_T
Cuts at: 6444 6444
Size: 6568
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2391 2832 2851 5682 5866 1305
Size: 1086 441 19 2831 184 2007
Fragments arranged by size:
2831 2007 1086 441 184 19
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2663 2891 3605 3707 10
Size: 54 1425 343 831 228 714 102 2871
Fragments arranged by size:
2871 1425 831 714 343 228 102 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2402 2420 3392 4526 4885 5044 518
Size: 648 1236 18 972 1134 359 159 2042
Fragments arranged by size:
2042 1236 1134 972 648 359 159 18
MspA1I CmG'CkG
Cuts at: 1606 2910 3586 3760 3984 4661 5809 6054 1606
Size: 1304 676 174 224 677 1148 245 2120
Fragments arranged by size:
2120 1304 1148 677 676 245 224 174
NaeI GCC'GGC
Cuts at: 3337 3337
Size: 6568
NcoI C'CATG_G
Cuts at: 513 1065 3144 3463 513
Size: 552 2079 319 3618
Fragments arranged by size:
3618 2079 552 319
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6568
NgoMIV G'CCGG_C
Cuts at: 3335 3335
Size: 6568
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6568
NotI GC'GGCC_GC
Cuts at: 4015 4015
Size: 6568
NspI r_CATG'y
Cuts at: 1132 1229 3335 3867 3926 5471 1132
Size: 97 2106 532 59 1545 2229
Fragments arranged by size:
2229 2106 1545 532 97 59
PasI CC'CwG_GG
Cuts at: 1348 3427 1348
Size: 2079 4489
PciI A'CATG_T
Cuts at: 5467 5467
Size: 6568
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2558 2640 3853 1108
Size: 1450 82 1213 3823
Fragments arranged by size:
3823 1450 1213 82
PmeI GTTT'AAAC
Cuts at: 3872 3872
Size: 6568
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4637 4669 6150 6182 4637
Size: 32 1481 32 5023
Fragments arranged by size:
5023 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2219 2219
Size: 6568
PshAI GACnn'nnGTC
Cuts at: 1613 2693 1613
Size: 1080 5488
PsiI TTA'TAA
Cuts at: 4138 4138
Size: 6568
PstI C_TGCA'G
Cuts at: 830 3201 3330 3920 830
Size: 2371 129 590 3478
Fragments arranged by size:
3478 2371 590 129
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6568
PvuII CAG'CTG
Cuts at: 3586 3760 3586
Size: 174 6394
RsrII CG'GwC_CG
Cuts at: 3534 3534
Size: 6568
SalI G'TCGA_C
Cuts at: 1936 3910 1936
Size: 1974 4594
SanDI GG'GwC_CC
Cuts at: 2219 2219
Size: 6568
SapI GCTCTTCn'nnn_
Cuts at: 2027 2303 2027
Size: 276 6292
SbfI CC_TGCA'GG
Cuts at: 3920 3920
Size: 6568
ScaI AGT'ACT
Cuts at: 4816 6528 4816
Size: 1712 4856
SfcI C'TryA_G
Cuts at: 826 1045 3197 3326 3916 5051 5732 5923 826
Size: 219 2152 129 590 1135 681 191 1471
Fragments arranged by size:
2152 1471 1135 681 590 219 191 129
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6568
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6568
SmaI CCC'GGG
Cuts at: 1872 3895 1872
Size: 2023 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2453 2948 3821 4672 5573 5835
Size: 197 926 510 495 873 851 901 262
Cuts at: 5835 820
Size: 1553
Fragments arranged by size:
1553 926 901 873 851 510 495 262
197
SnaBI TAC'GTA
Cuts at: 493 4359 493
Size: 3866 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6568
SphI G_CATG'C
Cuts at: 1132 3335 3926 1132
Size: 2203 591 3774
Fragments arranged by size:
3774 2203 591
SspI AAT'ATT
Cuts at: 5 52 2668 4492 5
Size: 47 2616 1824 2081
Fragments arranged by size:
2616 2081 1824 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6568
StyI C'CwwG_G
Cuts at: 513 1065 2137 2314 2634 2860 3144 3463 4007
Size: 552 1072 177 320 226 284 319 544
Cuts at: 4007 513
Size: 3074
Fragments arranged by size:
3074 1072 552 544 320 319 284 226
177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4595 4612 4765 4950 5395 325
Size: 1255 3015 17 153 185 445 1498
Fragments arranged by size:
3015 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2376 3306 4814
Size: 274 80 33 51 1443 399 930 1508
Cuts at: 4814 6526 96
Size: 1712 138
Fragments arranged by size:
1712 1508 1443 930 399 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3537 3569 6528 6560 3537
Size: 32 2959 32 3545
Fragments arranged by size:
3545 2959 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6568
XbaI T'CTAG_A
Cuts at: 3904 3904
Size: 6568
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2845 2845
Size: 6568
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6568
XmaI C'CCGG_G
Cuts at: 1870 3893 1870
Size: 2023 4545
XmnI GAAnn'nnTTC
Cuts at: 3155 3743 4697 3155
Size: 588 954 5026
Fragments arranged by size:
5026 954 588
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4376 276
Size: 53 83 186 936 2842 2468
Fragments arranged by size:
2842 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII
SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI Bpu10I BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI SacI SacII SexAI SfiI SfoI
SrfI SwaI