Restriction map for
pFN21AE6426
(Circular) (Six-base) MAPSORT of: pFN21AE6426.seq Check: 7247 from: 1 to: 6277
pFN21AE6426 6277 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4087 278
Size: 53 83 186 936 2551 2468
Fragments arranged by size:
2551 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2611 3321 3620 1937
Size: 674 710 299 4594
Fragments arranged by size:
4594 710 674 299
Acc65I G'GTAC_C
Cuts at: 3598 3598
Size: 6277
AclI AA'CG_TT
Cuts at: 1563 4404 4777 1563
Size: 2841 373 3063
Fragments arranged by size:
3063 2841 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2408 2504 2611 3110 4340 5724 5992 1632
Size: 776 96 107 499 1230 1384 268 1917
Fragments arranged by size:
1917 1384 1230 776 499 268 107 96
AfeI AGC'GCT
Cuts at: 3062 3062
Size: 6277
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6080
AflIII A'CryG_T
Cuts at: 1203 2574 5176 6153 1203
Size: 1371 2602 977 1327
Fragments arranged by size:
2602 1371 1327 977
AgeI A'CCGG_T
Cuts at: 2041 5108 2041
Size: 3067 3210
AhdI GACnn_n'nnGTC
Cuts at: 5006 5006
Size: 6277
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6277
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6277
AlwNI CAG_nnn'CTG
Cuts at: 2545 2781 2810 2969 3405 4020 5592 2545
Size: 236 29 159 436 615 1572 3230
Fragments arranged by size:
3230 1572 615 436 236 159 29
ApaI G_GGCC'C
Cuts at: 3399 3399
Size: 6277
ApaLI G'TGCA_C
Cuts at: 4334 5490 4334
Size: 1156 5121
ApoI r'AATT_y
Cuts at: 1493 3586 3812 3964 6073 1493
Size: 2093 226 152 2109 1697
Fragments arranged by size:
2109 2093 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4831 160
Size: 872 3799 1606
Fragments arranged by size:
3799 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6277
AvaI C'yCGr_G
Cuts at: 1870 1943 3602 1870
Size: 73 1659 4545
Fragments arranged by size:
4545 1659 73
BamHI G'GATC_C
Cuts at: 3310 3607 3974 3310
Size: 297 367 5613
Fragments arranged by size:
5613 367 297
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3042 3598 5053 618
Size: 325 217 627 1255 556 1455 1842
Fragments arranged by size:
1842 1455 1255 627 556 325 217
BanII G_rGCy'C
Cuts at: 1391 2087 2688 3399 3498 3558 3596 1391
Size: 696 601 711 99 60 38 4072
Fragments arranged by size:
4072 711 696 601 99 60 38
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5306
BbvCI CC'TCA_GC
Cuts at: 2344 2344
Size: 6277
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4468 4502 1903
Size: 34 2531 34 3678
Fragments arranged by size:
3678 2531 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2403 4169 5379 2403
Size: 1766 1210 3301
Fragments arranged by size:
3301 1766 1210
BclI T'GATC_A
Cuts at: 1548 2202 3516 1548
Size: 654 1314 4309
Fragments arranged by size:
4309 1314 654
BfrBI ATG'CAT
Cuts at: 2622 2622
Size: 6277
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4888 136
Size: 107 122 71 631 605 3216 1525
Fragments arranged by size:
3216 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6272 6272
Size: 6277
BlpI GC'TnA_GC
Cuts at: 2015 2915 3694 2015
Size: 900 779 4598
Fragments arranged by size:
4598 900 779
Bme1580I G_kGCm'C
Cuts at: 1790 2695 3399 4338 5494 1790
Size: 905 704 939 1156 2573
Fragments arranged by size:
2573 1156 939 905 704
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6277
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2652 4008 4966
Size: 416 53 83 399 24 1235 1356 958
Cuts at: 4966 442
Size: 1753
Fragments arranged by size:
1753 1356 1235 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6277
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3384 3384
Size: 6277
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2132 2708 4937 1239
Size: 893 576 2229 2579
Fragments arranged by size:
2579 2229 893 576
Bpu10I CC'TnA_GC
Cuts at: 2344 2344
Size: 6277
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4402 5267 5565 4402
Size: 865 298 5114
Fragments arranged by size:
5114 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4940 882
Size: 617 3441 2219
Fragments arranged by size:
3441 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4068 493
Size: 711 2864 2702
Fragments arranged by size:
2864 2702 711
BsaBI GATnn'nnATC
Cuts at: 6271 6271
Size: 6277
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2459 2827 4084 4466
Size: 53 83 186 936 926 368 1257 382
Cuts at: 4466 5101 275
Size: 635 1451
Fragments arranged by size:
1451 1257 936 926 635 382 368 186
83 53
BsaWI w'CCGG_w
Cuts at: 2041 2325 3471 4710 5108 5382 5529 5940 2041
Size: 284 1146 1239 398 274 147 411 2378
Fragments arranged by size:
2378 1239 1146 411 398 284 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6052
Fragments arranged by size:
6052 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2231 1186
Size: 1045 5232
BseYI C'CCAG_C
Cuts at: 2029 2278 2398 3132 3184 5480 5976 2029
Size: 249 120 734 52 2296 496 2330
Fragments arranged by size:
2330 2296 734 496 249 120 52
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3193 3449 1163
Size: 573 1457 256 3991
Fragments arranged by size:
3991 1457 573 256
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3727 4488 5516 1540
Size: 275 186 1726 761 1028 2301
Fragments arranged by size:
2301 1726 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2586 2688 4338 4423 5494 2586
Size: 102 1650 85 1071 3369
Fragments arranged by size:
3369 1650 1071 102 85
BsmI GAATG_Cn'
Cuts at: 3788 3881 3788
Size: 93 6184
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2087 2586 2688 2695 3399 3498 3558
Size: 399 297 499 102 7 704 99 60
Cuts at: 3558 3596 4338 4423 5494 1391
Size: 38 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 704 499 399 297 102
99 85 60 38 7
BspEI T'CCGG_A
Cuts at: 5940 5940
Size: 6277
BspHI T'CATG_A
Cuts at: 2595 4166 5896 2595
Size: 1571 1730 2976
Fragments arranged by size:
2976 1730 1571
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3269 3565 3632 844
Size: 2425 296 67 3489
Fragments arranged by size:
3489 2425 296 67
BsrBI CCG'CTC
Cuts at: 1394 1823 2824 4164 1394
Size: 429 1001 1340 3507
Fragments arranged by size:
3507 1340 1001 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2055 2352 4772 4946 65
Size: 1183 807 297 2420 174 1396
Fragments arranged by size:
2420 1396 1183 807 297 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2041 2142 4921 5108 1700
Size: 110 231 101 2779 187 2869
Fragments arranged by size:
2869 2779 231 187 110 101
BsrGI T'GTAC_A
Cuts at: 96 3027 96
Size: 2931 3346
BssHII G'CGCG_C
Cuts at: 1925 3523 1925
Size: 1598 4679
BssSI C'ACGA_G
Cuts at: 4337 5349 4337
Size: 1012 5265
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3261 794
Size: 2467 3810
BstBI TT'CG_AA
Cuts at: 5081 5081
Size: 6277
BstEII G'GTnAC_C
Cuts at: 1184 2189 3363 1184
Size: 1005 1174 4098
Fragments arranged by size:
4098 1174 1005
BstXI CCAn_nnnn'nTGG
Cuts at: 2680 2890 3303 2680
Size: 210 413 5654
Fragments arranged by size:
5654 413 210
BstYI r'GATC_y
Cuts at: 1972 3310 3607 3974 4359 4376 5828 5914 5926
Size: 1338 297 367 385 17 1452 86 12
Cuts at: 5926 6272 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1338 385 367 346 297 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3170 3315 3170
Size: 145 6132
BtgI C'CryG_G
Cuts at: 513 1065 2057 3000 3426 513
Size: 552 992 943 426 3364
Fragments arranged by size:
3364 992 943 552 426
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5538
Fragments arranged by size:
5538 683 56
BtsI GCAGTG_nn'
Cuts at: 3793 4587 4607 3793
Size: 794 20 5463
Fragments arranged by size:
5463 794 20
ClaI AT'CG_AT
Cuts at: 1554 3270 1554
Size: 1716 4561
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6242
DraI TTT'AAA
Cuts at: 3581 3581
Size: 6277
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2242 5284 809
Size: 507 926 3042 1802
Fragments arranged by size:
3042 1802 926 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2247 3003 3235
Size: 54 1425 50 275 18 417 756 232
Cuts at: 3235 3305 3724 4613 6242 8
Size: 70 419 889 1629 43
Fragments arranged by size:
1629 1425 889 756 419 417 275 232
70 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3724 1537
Size: 275 1912 4090
Fragments arranged by size:
4090 1912 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2378 4207 1353
Size: 1025 1829 3423
Fragments arranged by size:
3423 1829 1025
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3446 4864 5238 5384 387
Size: 1064 138 1857 1418 374 146 1280
Fragments arranged by size:
1857 1418 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2686 2686
Size: 6277
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2132 2408 2504 2611 2708 3110 4340
Size: 393 500 276 96 107 97 402 1230
Cuts at: 4340 4937 5724 5992 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1230 787 597 500 402 393 276
268 107 97 96
EcoO109I rG'GnC_Cy
Cuts at: 2282 2778 3022 3395 2282
Size: 496 244 373 5164
Fragments arranged by size:
5164 496 373 244
EcoRI G'AATT_C
Cuts at: 3586 3964 3586
Size: 378 5899
FspI TGC'GCA
Cuts at: 4783 4783
Size: 6277
HaeII r_GCGC'y
Cuts at: 1678 2770 3064 5424 1678
Size: 1092 294 2360 2531
Fragments arranged by size:
2531 2360 1092 294
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3163 3195 4918 4950 4992
Size: 32 135 32 1591 32 1723 32 42
Cuts at: 4992 5024 1373
Size: 32 2626
Fragments arranged by size:
2626 1723 1591 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3621 3867 669
Size: 988 281 1683 246 3079
Fragments arranged by size:
3079 1683 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3568 748
Size: 2820 3457
HpaI GTT'AAC
Cuts at: 3867 3867
Size: 6277
KpnI G_GTAC'C
Cuts at: 3602 3602
Size: 6277
MfeI C'AATT_G
Cuts at: 3876 3876
Size: 6277
MluI A'CGCG_T
Cuts at: 6153 6153
Size: 6277
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2429 5391 5575 1305
Size: 1124 2962 184 2007
Fragments arranged by size:
2962 2007 1124 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4455
Fragments arranged by size:
4455 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2903 2978 3155 4235 4594 4753 518
Size: 648 1737 75 177 1080 359 159 2042
Fragments arranged by size:
2042 1737 1080 648 359 177 159 75
MspA1I CmG'CkG
Cuts at: 1606 2348 3136 3693 4370 5518 5763 1606
Size: 742 788 557 677 1148 245 2120
Fragments arranged by size:
2120 1148 788 742 677 557 245
NaeI GCC'GGC
Cuts at: 2144 2144
Size: 6277
NcoI C'CATG_G
Cuts at: 513 1065 2057 3426 513
Size: 552 992 1369 3364
Fragments arranged by size:
3364 1369 992 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6277
NgoMIV G'CCGG_C
Cuts at: 2142 2142
Size: 6277
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6277
NotI GC'GGCC_GC
Cuts at: 3724 3724
Size: 6277
NsiI A_TGCA'T
Cuts at: 2624 2624
Size: 6277
NspI r_CATG'y
Cuts at: 1132 1229 2578 3635 5180 1132
Size: 97 1349 1057 1545 2229
Fragments arranged by size:
2229 1545 1349 1057 97
PasI CC'CwG_GG
Cuts at: 1348 2260 1348
Size: 912 5365
PciI A'CATG_T
Cuts at: 2574 5176 2574
Size: 2602 3675
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2731 1108
Size: 1623 4654
PfoI T'CCnGG_A
Cuts at: 2817 2817
Size: 6277
PmeI GTTT'AAAC
Cuts at: 3581 3581
Size: 6277
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4346 4378 5859 5891 4346
Size: 32 1481 32 4732
Fragments arranged by size:
4732 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2282 3022 2282
Size: 740 5537
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6277
PsiI TTA'TAA
Cuts at: 3847 3847
Size: 6277
PspOMI G'GGCC_C
Cuts at: 3395 3395
Size: 6277
PstI C_TGCA'G
Cuts at: 830 3629 830
Size: 2799 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6277
PvuII CAG'CTG
Cuts at: 2348 3136 2348
Size: 788 5489
SacI G_AGCT'C
Cuts at: 2688 2688
Size: 6277
SalI G'TCGA_C
Cuts at: 1936 3619 1936
Size: 1683 4594
SanDI GG'GwC_CC
Cuts at: 2282 2282
Size: 6277
SbfI CC_TGCA'GG
Cuts at: 3629 3629
Size: 6277
ScaI AGT'ACT
Cuts at: 4525 6237 4525
Size: 1712 4565
SfcI C'TryA_G
Cuts at: 826 1045 2211 2612 3625 4760 5441 5632 826
Size: 219 1166 401 1013 1135 681 191 1471
Fragments arranged by size:
1471 1166 1135 1013 681 401 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6277
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6277
SmaI CCC'GGG
Cuts at: 1872 3604 1872
Size: 1732 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4381 5282 5544 820
Size: 197 926 2438 901 262 1553
Fragments arranged by size:
2438 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4068 493
Size: 3575 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6277
SphI G_CATG'C
Cuts at: 1132 3635 1132
Size: 2503 3774
SspI AAT'ATT
Cuts at: 5 52 4201 5
Size: 47 4149 2081
Fragments arranged by size:
4149 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6277
StyI C'CwwG_G
Cuts at: 513 1065 2057 2361 2601 3426 3716 513
Size: 552 992 304 240 825 290 3074
Fragments arranged by size:
3074 992 825 552 304 290 240
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2374 3060 3227 4304 4321 4474 4659
Size: 1255 794 686 167 1077 17 153 185
Cuts at: 4659 5104 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1077 794 686 445 185 167
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3027 4523 6235
Size: 274 80 33 51 1443 1050 1496 1712
Cuts at: 6235 96
Size: 138
Fragments arranged by size:
1712 1496 1443 1050 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6237 6269 6237
Size: 32 6245
Tth111I GACn'n_nGTC
Cuts at: 1316 2810 1316
Size: 1494 4783
XbaI T'CTAG_A
Cuts at: 3613 3613
Size: 6277
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2175 2175
Size: 6277
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6277
XmaI C'CCGG_G
Cuts at: 1870 3602 1870
Size: 1732 4545
XmnI GAAnn'nnTTC
Cuts at: 4406 4406
Size: 6277
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4085 276
Size: 53 83 186 936 2551 2468
Fragments arranged by size:
2551 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BsiWI BsmBI
BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI
NarI NruI PacI PmlI PspXI PsrI RsrII SacII
SapI SexAI SfiI SfoI SrfI SwaI