Restriction map for
pFN21AE6425
(Circular) (Six-base) MAPSORT of: pFN21AE6425.seq Check: 9783 from: 1 to: 6034
pFN21AE6425 6034 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2926 2926
Size: 6034
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3844 278
Size: 53 83 186 936 2308 2468
Fragments arranged by size:
2468 2308 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3377 1937
Size: 1440 4594
Acc65I G'GTAC_C
Cuts at: 3355 3355
Size: 6034
AclI AA'CG_TT
Cuts at: 1563 4161 4534 1563
Size: 2598 373 3063
Fragments arranged by size:
3063 2598 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2643 4097 5481 5749 1632
Size: 1011 1454 1384 268 1917
Fragments arranged by size:
1917 1454 1384 1011 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5837
AflIII A'CryG_T
Cuts at: 1203 4933 5910 1203
Size: 3730 977 1327
Fragments arranged by size:
3730 1327 977
AgeI A'CCGG_T
Cuts at: 4865 4865
Size: 6034
AhdI GACnn_n'nnGTC
Cuts at: 4763 4763
Size: 6034
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6034
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2751 1184
Size: 1567 4467
AlwNI CAG_nnn'CTG
Cuts at: 2691 3777 5349 2691
Size: 1086 1572 3376
Fragments arranged by size:
3376 1572 1086
ApaLI G'TGCA_C
Cuts at: 3155 4091 5247 3155
Size: 936 1156 3942
Fragments arranged by size:
3942 1156 936
ApoI r'AATT_y
Cuts at: 1493 2934 3010 3343 3569 3721 5830 1493
Size: 1441 76 333 226 152 2109 1697
Fragments arranged by size:
2109 1697 1441 333 226 152 76
AseI AT'TA_AT
Cuts at: 160 1032 4588 160
Size: 872 3556 1606
Fragments arranged by size:
3556 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6034
AvaI C'yCGr_G
Cuts at: 1870 1943 3072 3359 1870
Size: 73 1129 287 4545
Fragments arranged by size:
4545 1129 287 73
BamHI G'GATC_C
Cuts at: 3364 3731 3364
Size: 367 5667
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3355 4810 618
Size: 325 217 627 1568 1455 1842
Fragments arranged by size:
1842 1568 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2721 3353 1391
Size: 1330 632 4072
Fragments arranged by size:
4072 1330 632
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2369 3292 928
Size: 971 470 923 3670
Fragments arranged by size:
3670 971 923 470
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3249 3283 4225 4259 1903
Size: 34 1312 34 942 34 3678
Fragments arranged by size:
3678 1312 942 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3926 5136 3926
Size: 1210 4824
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6034
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4645 136
Size: 107 122 71 631 605 2973 1525
Fragments arranged by size:
2973 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6029 6029
Size: 6034
BlpI GC'TnA_GC
Cuts at: 3451 3451
Size: 6034
Bme1580I G_kGCm'C
Cuts at: 1790 2765 3159 4095 5251 1790
Size: 975 394 936 1156 2573
Fragments arranged by size:
2573 1156 975 936 394
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6034
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3272 3765 4723
Size: 416 53 83 399 24 1855 493 958
Cuts at: 4723 442
Size: 1753
Fragments arranged by size:
1855 1753 958 493 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6034
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3068 3068
Size: 6034
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2735 2816 2949 4694 1239
Size: 1496 81 133 1745 2579
Fragments arranged by size:
2579 1745 1496 133 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4159 5024 5322 4159
Size: 865 298 4871
Fragments arranged by size:
4871 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4697 882
Size: 617 3198 2219
Fragments arranged by size:
3198 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3825 493
Size: 711 2621 2702
Fragments arranged by size:
2702 2621 711
BsaBI GATnn'nnATC
Cuts at: 6028 6028
Size: 6034
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3841 4223 4858 275
Size: 53 83 186 936 2308 382 635 1451
Fragments arranged by size:
2308 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2910 4467 4865 5139 5286 5697 2910
Size: 1557 398 274 147 411 3247
Fragments arranged by size:
3247 1557 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2117 2147 1178
Size: 30 165 30 714 30 5065
Fragments arranged by size:
5065 714 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2802 1186
Size: 1616 4418
BseYI C'CCAG_C
Cuts at: 2392 5237 5733 2392
Size: 2845 496 2693
Fragments arranged by size:
2845 2693 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5461
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3484 4245 5273 1540
Size: 275 186 1483 761 1028 2301
Fragments arranged by size:
2301 1483 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3159 4095 4180 5251 3159
Size: 936 85 1071 3942
Fragments arranged by size:
3942 1071 936 85
BsmI GAATG_Cn'
Cuts at: 3545 3638 3545
Size: 93 5941
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2721 2765 3159 3353 4095 4180 5251
Size: 399 931 44 394 194 742 85 1071
Cuts at: 5251 1391
Size: 2174
Fragments arranged by size:
2174 1071 931 742 399 394 194 85
44
BspEI T'CCGG_A
Cuts at: 5697 5697
Size: 6034
BspHI T'CATG_A
Cuts at: 3923 5653 3923
Size: 1730 4304
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2926 3389 844
Size: 2082 463 3489
Fragments arranged by size:
3489 2082 463
BsrBI CCG'CTC
Cuts at: 1394 1823 3921 1394
Size: 429 2098 3507
Fragments arranged by size:
3507 2098 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2733 2928 4529 4703 65
Size: 1183 1485 195 1601 174 1396
Fragments arranged by size:
1601 1485 1396 1183 195 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4678 4865 1700
Size: 110 2868 187 2869
Fragments arranged by size:
2869 2868 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6034
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6034
BssSI C'ACGA_G
Cuts at: 4094 5106 4094
Size: 1012 5022
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6034
BstBI TT'CG_AA
Cuts at: 3196 4838 3196
Size: 1642 4392
BstEII G'GTnAC_C
Cuts at: 1184 2913 1184
Size: 1729 4305
BstXI CCAn_nnnn'nTGG
Cuts at: 2755 2755
Size: 6034
BstYI r'GATC_y
Cuts at: 1972 2055 2894 3030 3364 3731 4116 4133 5585
Size: 83 839 136 334 367 385 17 1452
Cuts at: 5585 5671 5683 6029 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 839 385 367 346 334 136
86 83 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5482
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5295
Fragments arranged by size:
5295 683 56
BtsI GCAGTG_nn'
Cuts at: 3550 4344 4364 3550
Size: 794 20 5220
Fragments arranged by size:
5220 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6034
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5999
DraI TTT'AAA
Cuts at: 2298 2851 3338 2298
Size: 553 487 4994
Fragments arranged by size:
4994 553 487
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5041 809
Size: 507 3725 1802
Fragments arranged by size:
3725 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3481 4370 5999
Size: 54 1425 50 275 18 1651 889 1629
Cuts at: 5999 8
Size: 43
Fragments arranged by size:
1651 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3481 1537
Size: 275 1669 4090
Fragments arranged by size:
4090 1669 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2998 3964 1353
Size: 1645 966 3423
Fragments arranged by size:
3423 1645 966
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4621 4995 5141 387
Size: 1064 138 3032 374 146 1280
Fragments arranged by size:
3032 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2643 2735 2816 2949 4097 4694 5481
Size: 393 1011 92 81 133 1148 597 787
Cuts at: 5481 5749 1239
Size: 268 1524
Fragments arranged by size:
1524 1148 1011 787 597 393 268 133
92 81
EcoO109I rG'GnC_Cy
Cuts at: 2040 2743 2040
Size: 703 5331
EcoRI G'AATT_C
Cuts at: 3343 3721 3343
Size: 378 5656
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2844 2844
Size: 6034
FspI TGC'GCA
Cuts at: 4540 4540
Size: 6034
HaeII r_GCGC'y
Cuts at: 1678 5181 1678
Size: 3503 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2117 2149 4675 4707 4749
Size: 32 135 32 545 32 2526 32 42
Cuts at: 4749 4781 1373
Size: 32 2626
Fragments arranged by size:
2626 2526 545 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3378 3624 669
Size: 988 281 1440 246 3079
Fragments arranged by size:
3079 1440 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2329 748
Size: 1581 4453
HpaI GTT'AAC
Cuts at: 3624 3624
Size: 6034
KpnI G_GTAC'C
Cuts at: 3359 3359
Size: 6034
MfeI C'AATT_G
Cuts at: 3633 3633
Size: 6034
MluI A'CGCG_T
Cuts at: 5910 5910
Size: 6034
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3291 5148 5332 1305
Size: 1986 1857 184 2007
Fragments arranged by size:
2007 1986 1857 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4212
Fragments arranged by size:
4212 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2094 3280 3992 4351 4510 518
Size: 648 928 1186 712 359 159 2042
Fragments arranged by size:
2042 1186 928 712 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2106 3450 4127 5275 5520 1606
Size: 500 1344 677 1148 245 2120
Fragments arranged by size:
2120 1344 1148 677 500 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5482
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6034
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6034
NotI GC'GGCC_GC
Cuts at: 3481 3481
Size: 6034
NruI TCG'CGA
Cuts at: 3237 3237
Size: 6034
NspI r_CATG'y
Cuts at: 1132 1229 2732 3392 4937 1132
Size: 97 1503 660 1545 2229
Fragments arranged by size:
2229 1545 1503 660 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6034
PciI A'CATG_T
Cuts at: 4933 4933
Size: 6034
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6034
PfoI T'CCnGG_A
Cuts at: 2679 2679
Size: 6034
PmeI GTTT'AAAC
Cuts at: 3338 3338
Size: 6034
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4103 4135 5616 5648 4103
Size: 32 1481 32 4489
Fragments arranged by size:
4489 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2040 2743 2040
Size: 703 5331
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6034
PsiI TTA'TAA
Cuts at: 3059 3604 3059
Size: 545 5489
PstI C_TGCA'G
Cuts at: 830 3386 830
Size: 2556 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6034
PvuII CAG'CTG
Cuts at: 2106 2106
Size: 6034
SalI G'TCGA_C
Cuts at: 1936 3376 1936
Size: 1440 4594
SbfI CC_TGCA'GG
Cuts at: 3386 3386
Size: 6034
ScaI AGT'ACT
Cuts at: 4282 5994 4282
Size: 1712 4322
SfcI C'TryA_G
Cuts at: 826 1045 3382 4517 5198 5389 826
Size: 219 2337 1135 681 191 1471
Fragments arranged by size:
2337 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6034
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6034
SmaI CCC'GGG
Cuts at: 1872 3361 1872
Size: 1489 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3072 4138 5039 5301 820
Size: 197 926 1129 1066 901 262 1553
Fragments arranged by size:
1553 1129 1066 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3825 493
Size: 3332 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6034
SphI G_CATG'C
Cuts at: 1132 2732 3392 1132
Size: 1600 660 3774
Fragments arranged by size:
3774 1600 660
SspI AAT'ATT
Cuts at: 5 52 3232 3958 5
Size: 47 3180 726 2081
Fragments arranged by size:
3180 2081 726 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6034
StyI C'CwwG_G
Cuts at: 513 1065 2739 3473 513
Size: 552 1674 734 3074
Fragments arranged by size:
3074 1674 734 552
SwaI ATTT'AAAT
Cuts at: 2851 2851
Size: 6034
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4061 4078 4231 4416 4861 325
Size: 1255 2481 17 153 185 445 1498
Fragments arranged by size:
2481 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4280 5992 96
Size: 274 80 33 51 1443 2303 1712 138
Fragments arranged by size:
2303 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2142 2174 2298 2330 5994 6026 2142
Size: 32 124 32 3664 32 2150
Fragments arranged by size:
3664 2150 124 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2464 1316
Size: 1148 4886
XbaI T'CTAG_A
Cuts at: 3370 3370
Size: 6034
XhoI C'TCGA_G
Cuts at: 1943 3072 1943
Size: 1129 4905
XmaI C'CCGG_G
Cuts at: 1870 3359 1870
Size: 1489 4545
XmnI GAAnn'nnTTC
Cuts at: 2577 3109 4163 2577
Size: 532 1054 4448
Fragments arranged by size:
4448 1054 532
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3842 276
Size: 53 83 186 936 2308 2468
Fragments arranged by size:
2468 2308 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NruI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI XcmI