Restriction map for
pFN21AE6424
(Circular) (Six-base) MAPSORT of: pFN21AE6424.seq Check: 672 from: 1 to: 6364
pFN21AE6424 6364 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3398 3398
Size: 6364
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4174 278
Size: 53 83 186 936 2638 2468
Fragments arranged by size:
2638 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3414 3707 1937
Size: 1477 293 4594
Fragments arranged by size:
4594 1477 293
Acc65I G'GTAC_C
Cuts at: 3685 3685
Size: 6364
AclI AA'CG_TT
Cuts at: 1563 4491 4864 1563
Size: 2928 373 3063
Fragments arranged by size:
3063 2928 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2087 2180 2784 3115 3662 4427 5811 6079
Size: 455 93 604 331 547 765 1384 268
Cuts at: 6079 1632
Size: 1917
Fragments arranged by size:
1917 1384 765 604 547 455 331 268
93
AfeI AGC'GCT
Cuts at: 2596 3164 2596
Size: 568 5796
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6167
AflIII A'CryG_T
Cuts at: 1203 2118 3483 5263 6240 1203
Size: 915 1365 1780 977 1327
Fragments arranged by size:
1780 1365 1327 977 915
AgeI A'CCGG_T
Cuts at: 5195 5195
Size: 6364
AhdI GACnn_n'nnGTC
Cuts at: 5093 5093
Size: 6364
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6364
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2114 2306 2486 1184
Size: 930 192 180 5062
Fragments arranged by size:
5062 930 192 180
AlwNI CAG_nnn'CTG
Cuts at: 2110 2479 3524 3554 4107 5679 2110
Size: 369 1045 30 553 1572 2795
Fragments arranged by size:
2795 1572 1045 553 369 30
ApaI G_GGCC'C
Cuts at: 2673 2768 2778 2673
Size: 95 10 6259
Fragments arranged by size:
6259 95 10
ApaLI G'TGCA_C
Cuts at: 3383 4421 5577 3383
Size: 1038 1156 4170
Fragments arranged by size:
4170 1156 1038
ApoI r'AATT_y
Cuts at: 1493 3673 3899 4051 6160 1493
Size: 2180 226 152 2109 1697
Fragments arranged by size:
2180 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4918 160
Size: 872 3886 1606
Fragments arranged by size:
3886 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6364
AvaI C'yCGr_G
Cuts at: 1870 1943 2308 2740 3284 3689 1870
Size: 73 365 432 544 405 4545
Fragments arranged by size:
4545 544 432 405 365 73
BamHI G'GATC_C
Cuts at: 2872 2908 3694 4061 2872
Size: 36 786 367 5175
Fragments arranged by size:
5175 786 367 36
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2064 2239 2710 3628 3685
Size: 325 217 627 277 175 471 918 57
Cuts at: 3685 5140 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 918 627 471 325 277 217
175 57
BanII G_rGCy'C
Cuts at: 1391 2564 2625 2673 2768 2778 2955 3585 3683
Size: 1173 61 48 95 10 177 630 98
Cuts at: 3683 1391
Size: 4072
Fragments arranged by size:
4072 1173 630 177 98 95 61 48
10
BbeI G_GCGC'C
Cuts at: 2068 2068
Size: 6364
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3070 928
Size: 971 1171 4222
Fragments arranged by size:
4222 1171 971
BbvCI CC'TCA_GC
Cuts at: 3555 3555
Size: 6364
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2077 2111 4555 4589 1903
Size: 34 140 34 2444 34 3678
Fragments arranged by size:
3678 2444 140 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4256 5466 4256
Size: 1210 5154
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6364
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2782 2835
Size: 107 122 71 631 605 337 773 53
Cuts at: 2835 3637 4975 136
Size: 802 1338 1525
Fragments arranged by size:
1525 1338 802 773 631 605 337 122
107 71 53
BglII A'GATC_T
Cuts at: 6359 6359
Size: 6364
BlpI GC'TnA_GC
Cuts at: 2624 3781 2624
Size: 1157 5207
Bme1580I G_kGCm'C
Cuts at: 1790 2244 2465 2673 2713 2768 2778 3387 3633
Size: 454 221 208 40 55 10 609 246
Cuts at: 3633 4425 5581 1790
Size: 792 1156 2573
Fragments arranged by size:
2573 1156 792 609 454 246 221 208
55 40 10
BmgBI CAC'GTC
Cuts at: 1318 3058 3466 1318
Size: 1740 408 4216
Fragments arranged by size:
4216 1740 408
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2405 4095 5053
Size: 416 53 83 399 24 988 1690 958
Cuts at: 5053 442
Size: 1753
Fragments arranged by size:
1753 1690 988 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6364
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2132 2177 2252 2809 2853 3011 3179 3440
Size: 893 45 75 557 44 158 168 261
Cuts at: 3440 3455 5024 1239
Size: 15 1569 2579
Fragments arranged by size:
2579 1569 893 557 261 168 158 75
45 44 15
Bpu10I CC'TnA_GC
Cuts at: 3555 3579 3555
Size: 24 6340
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2659 3065 4489 5354 5652 2659
Size: 406 1424 865 298 3371
Fragments arranged by size:
3371 1424 865 406 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5027 882
Size: 617 3528 2219
Fragments arranged by size:
3528 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2121 4155 493
Size: 711 917 2034 2702
Fragments arranged by size:
2702 2034 917 711
BsaBI GATnn'nnATC
Cuts at: 6358 6358
Size: 6364
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2065 2084 4171 4553
Size: 53 83 186 936 532 19 2087 382
Cuts at: 4553 5188 275
Size: 635 1451
Fragments arranged by size:
2087 1451 936 635 532 382 186 83
53 19
BsaWI w'CCGG_w
Cuts at: 2647 4797 5195 5469 5616 6027 2647
Size: 2150 398 274 147 411 2984
Fragments arranged by size:
2984 2150 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2105 2135 1178
Size: 30 165 30 702 30 5407
Fragments arranged by size:
5407 702 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2185 3008 3280 3413 3572 3628 1186
Size: 999 823 272 133 159 56 3922
Fragments arranged by size:
3922 999 823 272 159 133 56
BseYI C'CCAG_C
Cuts at: 2479 2877 2913 3075 3230 3600 5567 6063 2479
Size: 398 36 162 155 370 1967 496 2780
Fragments arranged by size:
2780 1967 496 398 370 162 155 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3516 1163
Size: 573 1780 4011
Fragments arranged by size:
4011 1780 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2062 3202 3375 3462 3814 4575
Size: 275 186 61 1140 173 87 352 761
Cuts at: 4575 5603 1540
Size: 1028 2301
Fragments arranged by size:
2301 1140 1028 761 352 275 186 173
87 61
BsiHKAI G_wGCw'C
Cuts at: 2093 2181 2625 2685 2705 3387 4425 4510 5581
Size: 88 444 60 20 682 1038 85 1071
Cuts at: 5581 2093
Size: 2876
Fragments arranged by size:
2876 1071 1038 682 444 88 85 60
20
BsmI GAATG_Cn'
Cuts at: 3875 3968 3875
Size: 93 6271
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2093 2181 2244 2465 2564 2625 2673
Size: 399 303 88 63 221 99 61 48
Cuts at: 2673 2685 2705 2713 2768 2778 2955 3387 3585
Size: 12 20 8 55 10 177 432 198
Cuts at: 3585 3633 3683 4425 4510 5581 1391
Size: 48 50 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 432 399 303 221 198
177 99 88 85 63 61 55 50
48 48 20 12 10 8
BspEI T'CCGG_A
Cuts at: 2647 6027 2647
Size: 3380 2984
BspHI T'CATG_A
Cuts at: 2517 4253 5983 2517
Size: 1736 1730 2898
Fragments arranged by size:
2898 1736 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2220 3398 3719 844
Size: 1376 1178 321 3489
Fragments arranged by size:
3489 1376 1178 321
BsrBI CCG'CTC
Cuts at: 1394 1823 2899 2905 2929 2935 2998 3091 3377
Size: 429 1076 6 24 6 63 93 286
Cuts at: 3377 4251 1394
Size: 874 3507
Fragments arranged by size:
3507 1076 874 429 286 93 63 24
6 6
BsrDI GCAATG_nn'
Cuts at: 65 1248 3512 4859 5033 65
Size: 1183 2264 1347 174 1396
Fragments arranged by size:
2264 1396 1347 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2061 2124 2968 3403 3649 5008 5195
Size: 110 251 63 844 435 246 1359 187
Cuts at: 5195 1700
Size: 2869
Fragments arranged by size:
2869 1359 844 435 251 246 187 110
63
BsrGI T'GTAC_A
Cuts at: 96 2421 96
Size: 2325 4039
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6364
BssSI C'ACGA_G
Cuts at: 2425 2779 4424 5436 2425
Size: 354 1645 1012 3353
Fragments arranged by size:
3353 1645 1012 354
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6364
BstBI TT'CG_AA
Cuts at: 5168 5168
Size: 6364
BstEII G'GTnAC_C
Cuts at: 1184 3118 1184
Size: 1934 4430
BstYI r'GATC_y
Cuts at: 1972 2872 2908 3694 4061 4446 4463 5915 6001
Size: 900 36 786 367 385 17 1452 86
Cuts at: 6001 6013 6359 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 900 786 385 367 346 86
36 17 12
Bsu36I CC'TnA_GG
Cuts at: 2665 2665
Size: 6364
BtgI C'CryG_G
Cuts at: 513 1065 2004 2034 2535 2629 2940 2955 513
Size: 552 939 30 501 94 311 15 3922
Fragments arranged by size:
3922 939 552 501 311 94 30 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2269 487
Size: 683 56 1043 4582
Fragments arranged by size:
4582 1043 683 56
BtsI GCAGTG_nn'
Cuts at: 3880 4674 4694 3880
Size: 794 20 5550
Fragments arranged by size:
5550 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6364
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2589 2624 529
Size: 35 2025 35 4269
Fragments arranged by size:
4269 2025 35 35
DraI TTT'AAA
Cuts at: 3668 3668
Size: 6364
DraIII CAC_nnn'GTG
Cuts at: 2074 2074
Size: 6364
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2473 3206 5371 809
Size: 507 1157 733 2165 1802
Fragments arranged by size:
2165 1802 1157 733 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2059 2578 3011
Size: 54 1425 50 275 18 229 519 433
Cuts at: 3011 3372 3459 3474 3651 3811 4700 6329 8
Size: 361 87 15 177 160 889 1629 43
Fragments arranged by size:
1629 1425 889 519 433 361 275 229
177 160 87 54 50 43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 2059 3372 3459 3811 1537
Size: 275 247 1313 87 352 4090
Fragments arranged by size:
4090 1313 352 275 247 87
EarI CTCTTCn'nnn_
Cuts at: 1353 3371 3605 4294 1353
Size: 2018 234 689 3423
Fragments arranged by size:
3423 2018 689 234
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2040 3215 4951 5325 5471 387
Size: 1064 138 451 1175 1736 374 146 1280
Fragments arranged by size:
1736 1280 1175 1064 451 374 146 138
EcoICRI GAG'CTC
Cuts at: 2623 2623
Size: 6364
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2087 2132 2177 2180 2252 2784 2809
Size: 393 455 45 45 3 72 532 25
Cuts at: 2809 2853 3011 3115 3179 3440 3455 3662 4427
Size: 44 158 104 64 261 15 207 765
Cuts at: 4427 5024 5811 6079 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 765 597 532 455 393 268
261 207 158 104 72 64 45 45
44 25 15 3
EcoNI CCTnn'n_nnAGG
Cuts at: 3353 3623 3353
Size: 270 6094
EcoO109I rG'GnC_Cy
Cuts at: 2264 2669 2670 2764 2765 2837 3187 3501 2264
Size: 405 1 94 1 72 350 314 5127
Fragments arranged by size:
5127 405 350 314 94 72 1 1
EcoRI G'AATT_C
Cuts at: 3673 4051 3673
Size: 378 5986
FspI TGC'GCA
Cuts at: 4870 4870
Size: 6364
HaeII r_GCGC'y
Cuts at: 1678 2068 2502 2598 3100 3166 3569 5511 1678
Size: 390 434 96 502 66 403 1942 2531
Fragments arranged by size:
2531 1942 502 434 403 390 96 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2291 2323 5005 5037 5079
Size: 32 135 32 719 32 2682 32 42
Cuts at: 5079 5111 1373
Size: 32 2626
Fragments arranged by size:
2682 2626 719 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3708 3954 669
Size: 988 281 1770 246 3079
Fragments arranged by size:
3079 1770 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6364
HpaI GTT'AAC
Cuts at: 3954 3954
Size: 6364
KasI G'GCGC_C
Cuts at: 2064 2064
Size: 6364
KpnI G_GTAC'C
Cuts at: 3689 3689
Size: 6364
MfeI C'AATT_G
Cuts at: 3963 3963
Size: 6364
MluI A'CGCG_T
Cuts at: 6240 6240
Size: 6364
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5478 5662 1305
Size: 4173 184 2007
Fragments arranged by size:
4173 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2580 3013 3476 10
Size: 54 1425 343 748 433 463 2898
Fragments arranged by size:
2898 1425 748 463 433 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4322 4681 4840 518
Size: 648 3156 359 159 2042
Fragments arranged by size:
3156 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2036 2296 2631 2720 3017 3551 3780 4457
Size: 430 260 335 89 297 534 229 677
Cuts at: 4457 5605 5850 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 534 430 335 297 260
245 229 89
NaeI GCC'GGC
Cuts at: 2063 2126 3405 3651 2063
Size: 63 1279 246 4776
Fragments arranged by size:
4776 1279 246 63
NarI GG'CG_CC
Cuts at: 2065 2065
Size: 6364
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4873
Fragments arranged by size:
4873 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6364
NgoMIV G'CCGG_C
Cuts at: 2061 2124 3403 3649 2061
Size: 63 1279 246 4776
Fragments arranged by size:
4776 1279 246 63
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6364
NotI GC'GGCC_GC
Cuts at: 3811 3811
Size: 6364
NspI r_CATG'y
Cuts at: 1132 1229 3443 3487 3722 5267 1132
Size: 97 2214 44 235 1545 2229
Fragments arranged by size:
2229 2214 1545 235 97 44
PasI CC'CwG_GG
Cuts at: 1348 3590 1348
Size: 2242 4122
PciI A'CATG_T
Cuts at: 3483 5263 3483
Size: 1780 4584
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6364
PfoI T'CCnGG_A
Cuts at: 2249 3327 2249
Size: 1078 5286
PmeI GTTT'AAAC
Cuts at: 3668 3668
Size: 6364
PmlI CAC'GTG
Cuts at: 2121 2121
Size: 6364
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4433 4465 5946 5978 4433
Size: 32 1481 32 4819
Fragments arranged by size:
4819 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3187 3501 3187
Size: 314 6050
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6364
PsiI TTA'TAA
Cuts at: 3934 3934
Size: 6364
PspOMI G'GGCC_C
Cuts at: 2669 2764 2774 2669
Size: 95 10 6259
Fragments arranged by size:
6259 95 10
PspXI vC'TCGA_Gb
Cuts at: 2308 2308
Size: 6364
PstI C_TGCA'G
Cuts at: 830 2391 2475 3006 3716 830
Size: 1561 84 531 710 3478
Fragments arranged by size:
3478 1561 710 531 84
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6364
PvuII CAG'CTG
Cuts at: 2296 2720 3551 2296
Size: 424 831 5109
Fragments arranged by size:
5109 831 424
SacI G_AGCT'C
Cuts at: 2625 2625
Size: 6364
SacII CC_GC'GG
Cuts at: 2037 2632 2037
Size: 595 5769
SalI G'TCGA_C
Cuts at: 1936 3706 1936
Size: 1770 4594
SanDI GG'GwC_CC
Cuts at: 3501 3501
Size: 6364
SbfI CC_TGCA'GG
Cuts at: 3716 3716
Size: 6364
ScaI AGT'ACT
Cuts at: 4612 6324 4612
Size: 1712 4652
SfcI C'TryA_G
Cuts at: 826 1045 2387 2471 3002 3212 3712 4847 5528
Size: 219 1342 84 531 210 500 1135 681
Cuts at: 5528 5719 826
Size: 191 1471
Fragments arranged by size:
1471 1342 1135 681 531 500 219 210
191 84
SfiI GGCCn_nnn'nGGCC
Cuts at: 2782 2782
Size: 6364
SfoI GGC'GCC
Cuts at: 2066 2066
Size: 6364
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6364
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6364
SmaI CCC'GGG
Cuts at: 1872 3691 1872
Size: 1819 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2308 2638 3080 4468 5369 5631
Size: 197 926 365 330 442 1388 901 262
Cuts at: 5631 820
Size: 1553
Fragments arranged by size:
1553 1388 926 901 442 365 330 262
197
SnaBI TAC'GTA
Cuts at: 493 4155 493
Size: 3662 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6364
SphI G_CATG'C
Cuts at: 1132 3443 3722 1132
Size: 2311 279 3774
Fragments arranged by size:
3774 2311 279
SspI AAT'ATT
Cuts at: 5 52 4288 5
Size: 47 4236 2081
Fragments arranged by size:
4236 2081 47
StuI AGG'CCT
Cuts at: 1517 2785 3008 3128 1517
Size: 1268 223 120 4753
Fragments arranged by size:
4753 1268 223 120
StyI C'CwwG_G
Cuts at: 513 1065 2004 2607 3114 3123 3803 513
Size: 552 939 603 507 9 680 3074
Fragments arranged by size:
3074 939 680 603 552 507 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4391 4408 4561 4746 5191 325
Size: 1255 2811 17 153 185 445 1498
Fragments arranged by size:
2811 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2421 4610 6322
Size: 274 80 33 51 1443 444 2189 1712
Cuts at: 6322 96
Size: 138
Fragments arranged by size:
2189 1712 1443 444 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2105 2137 6324 6356 2105
Size: 32 4187 32 2113
Fragments arranged by size:
4187 2113 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6364
XbaI T'CTAG_A
Cuts at: 3700 3700
Size: 6364
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2233 3238 2233
Size: 1005 5359
XhoI C'TCGA_G
Cuts at: 1943 2308 1943
Size: 365 5999
XmaI C'CCGG_G
Cuts at: 1870 3689 1870
Size: 1819 4545
XmnI GAAnn'nnTTC
Cuts at: 3365 4493 3365
Size: 1128 5236
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4172 276
Size: 53 83 186 936 2638 2468
Fragments arranged by size:
2638 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SacII SalI SanDI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstXI BstZ17I EcoRV FalI FseI FspAI NruI NsiI
PacI PsrI RsrII SapI SexAI SrfI SwaI