Restriction map for
pFN21AE6415
(Circular) (Six-base) MAPSORT of: pFN21AE6415.seq Check: 3214 from: 1 to: 6145
pFN21AE6415 6145 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2317 3955 278
Size: 53 83 186 936 781 1638 2468
Fragments arranged by size:
2468 1638 936 781 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3488 1937
Size: 1551 4594
Acc65I G'GTAC_C
Cuts at: 3466 3466
Size: 6145
AclI AA'CG_TT
Cuts at: 1563 4272 4645 1563
Size: 2709 373 3063
Fragments arranged by size:
3063 2709 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2286 4208 5592 5860 1632
Size: 654 1922 1384 268 1917
Fragments arranged by size:
1922 1917 1384 654 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5948
AflIII A'CryG_T
Cuts at: 1203 5044 6021 1203
Size: 3841 977 1327
Fragments arranged by size:
3841 1327 977
AgeI A'CCGG_T
Cuts at: 4976 4976
Size: 6145
AhdI GACnn_n'nnGTC
Cuts at: 4874 4874
Size: 6145
AleI CACnn'nnGTG
Cuts at: 1166 2820 1166
Size: 1654 4491
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6145
AlwNI CAG_nnn'CTG
Cuts at: 3888 5460 3888
Size: 1572 4573
ApaLI G'TGCA_C
Cuts at: 2658 4202 5358 2658
Size: 1544 1156 3445
Fragments arranged by size:
3445 1544 1156
ApoI r'AATT_y
Cuts at: 1493 2472 3162 3380 3454 3680 3832 5941 1493
Size: 979 690 218 74 226 152 2109 1697
Fragments arranged by size:
2109 1697 979 690 226 218 152 74
AseI AT'TA_AT
Cuts at: 160 1032 4699 160
Size: 872 3667 1606
Fragments arranged by size:
3667 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6145
AvaI C'yCGr_G
Cuts at: 1870 1943 3470 1870
Size: 73 1527 4545
Fragments arranged by size:
4545 1527 73
BamHI G'GATC_C
Cuts at: 3475 3842 3475
Size: 367 5778
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3035 3466 4921 618
Size: 325 217 627 1248 431 1455 1842
Fragments arranged by size:
1842 1455 1248 627 431 325 217
BanII G_rGCy'C
Cuts at: 1391 2677 3093 3335 3464 1391
Size: 1286 416 242 129 4072
Fragments arranged by size:
4072 1286 416 242 129
BbeI G_GCGC'C
Cuts at: 3039 3039
Size: 6145
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3264 928
Size: 971 1365 3809
Fragments arranged by size:
3809 1365 971
BbvCI CC'TCA_GC
Cuts at: 3030 3030
Size: 6145
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4336 4370 1903
Size: 34 2399 34 3678
Fragments arranged by size:
3678 2399 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4037 5247 4037
Size: 1210 4935
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6145
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2063 2261 4756
Size: 107 122 71 631 605 391 198 2495
Cuts at: 4756 136
Size: 1525
Fragments arranged by size:
2495 1525 631 605 391 198 122 107
71
BglII A'GATC_T
Cuts at: 2121 3000 3141 6140 2121
Size: 879 141 2999 2126
Fragments arranged by size:
2999 2126 879 141
BlpI GC'TnA_GC
Cuts at: 3562 3562
Size: 6145
Bme1580I G_kGCm'C
Cuts at: 1790 2662 4206 5362 1790
Size: 872 1544 1156 2573
Fragments arranged by size:
2573 1544 1156 872
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6145
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3074 3876 4834
Size: 416 53 83 399 24 1657 802 958
Cuts at: 4834 442
Size: 1753
Fragments arranged by size:
1753 1657 958 802 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6145
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2757 2757
Size: 6145
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2583 4805 1239
Size: 1344 2222 2579
Fragments arranged by size:
2579 2222 1344
Bpu10I CC'TnA_GC
Cuts at: 3030 3030
Size: 6145
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4270 5135 5433 4270
Size: 865 298 4982
Fragments arranged by size:
4982 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4808 882
Size: 617 3309 2219
Fragments arranged by size:
3309 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3936 493
Size: 711 2732 2702
Fragments arranged by size:
2732 2702 711
BsaBI GATnn'nnATC
Cuts at: 6139 6139
Size: 6145
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2066 2314 2430 3036
Size: 53 83 186 936 533 248 116 606
Cuts at: 3036 3952 4334 4969 275
Size: 916 382 635 1451
Fragments arranged by size:
1451 936 916 635 606 533 382 248
186 116 83 53
BsaWI w'CCGG_w
Cuts at: 4578 4976 5250 5397 5808 4578
Size: 398 274 147 411 4915
Fragments arranged by size:
4915 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2184 2214 1178
Size: 30 165 30 781 30 5109
Fragments arranged by size:
5109 781 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2891 2906 3152 1186
Size: 1705 15 246 4179
Fragments arranged by size:
4179 1705 246 15
BseYI C'CCAG_C
Cuts at: 2460 3287 5348 5844 2460
Size: 827 2061 496 2761
Fragments arranged by size:
2761 2061 827 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2057 2267 2833 1163
Size: 573 321 210 566 4475
Fragments arranged by size:
4475 573 566 321 210
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3595 4356 5384 1540
Size: 275 186 1594 761 1028 2301
Fragments arranged by size:
2301 1594 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2662 4206 4291 5362 2662
Size: 1544 85 1071 3445
Fragments arranged by size:
3445 1544 1071 85
BsmI GAATG_Cn'
Cuts at: 3656 3749 3656
Size: 93 6052
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2662 2677 3093 3335 3464 4206 4291
Size: 399 872 15 416 242 129 742 85
Cuts at: 4291 5362 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 872 742 416 399 242 129
85 15
BspEI T'CCGG_A
Cuts at: 5808 5808
Size: 6145
BspHI T'CATG_A
Cuts at: 4034 5764 4034
Size: 1730 4415
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3500 844
Size: 2656 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2917 4032 1394
Size: 429 1094 1115 3507
Fragments arranged by size:
3507 1115 1094 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3285 4640 4814 65
Size: 1183 2037 1355 174 1396
Fragments arranged by size:
2037 1396 1355 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4789 4976 1700
Size: 110 2979 187 2869
Fragments arranged by size:
2979 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6145
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6145
BssSI C'ACGA_G
Cuts at: 4205 5217 4205
Size: 1012 5133
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2080 2363 794
Size: 1286 283 4576
Fragments arranged by size:
4576 1286 283
BstBI TT'CG_AA
Cuts at: 4949 4949
Size: 6145
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6145
BstYI r'GATC_y
Cuts at: 1972 2121 3000 3141 3426 3475 3842 4227 4244
Size: 149 879 141 285 49 367 385 17
Cuts at: 4244 5696 5782 5794 6140 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 879 385 367 346 285 149
141 86 49 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2370 2558 513
Size: 552 1305 188 4100
Fragments arranged by size:
4100 1305 552 188
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5406
Fragments arranged by size:
5406 683 56
BtsI GCAGTG_nn'
Cuts at: 2250 2850 3661 4455 4475 2250
Size: 600 811 794 20 3920
Fragments arranged by size:
3920 811 794 600 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6145
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6110
DraI TTT'AAA
Cuts at: 3449 3449
Size: 6145
DraIII CAC_nnn'GTG
Cuts at: 2657 2657
Size: 6145
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5152 809
Size: 507 3836 1802
Fragments arranged by size:
3836 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3592 4481 6110
Size: 54 1425 50 275 18 1762 889 1629
Cuts at: 6110 8
Size: 43
Fragments arranged by size:
1762 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3592 1537
Size: 275 1780 4090
Fragments arranged by size:
4090 1780 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2726 4075 1353
Size: 1373 1349 3423
Fragments arranged by size:
3423 1373 1349
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4732 5106 5252 387
Size: 1064 138 3143 374 146 1280
Fragments arranged by size:
3143 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2286 2583 4208 4805 5592 5860 1239
Size: 393 654 297 1625 597 787 268 1524
Fragments arranged by size:
1625 1524 787 654 597 393 297 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2681 2681
Size: 6145
EcoO109I rG'GnC_Cy
Cuts at: 2969 3259 2969
Size: 290 5855
EcoRI G'AATT_C
Cuts at: 3454 3832 3454
Size: 378 5767
FspI TGC'GCA
Cuts at: 4651 4651
Size: 6145
HaeII r_GCGC'y
Cuts at: 1678 3039 5292 1678
Size: 1361 2253 2531
Fragments arranged by size:
2531 2253 1361
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2538 2570 2664 2696 4786
Size: 32 135 32 966 32 94 32 2090
Cuts at: 4786 4818 4860 4892 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2090 966 135 94 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3489 3735 669
Size: 988 281 1551 246 3079
Fragments arranged by size:
3079 1551 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2327 2406 2696 748
Size: 1579 79 290 4197
Fragments arranged by size:
4197 1579 290 79
HpaI GTT'AAC
Cuts at: 3735 3735
Size: 6145
KasI G'GCGC_C
Cuts at: 3035 3035
Size: 6145
KpnI G_GTAC'C
Cuts at: 3470 3470
Size: 6145
MfeI C'AATT_G
Cuts at: 3304 3744 3304
Size: 440 5705
MluI A'CGCG_T
Cuts at: 6021 6021
Size: 6145
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2301 2671 3129 5259 5443 1305
Size: 996 370 458 2130 184 2007
Fragments arranged by size:
2130 2007 996 458 370 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4323
Fragments arranged by size:
4323 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2537 2820 2870 4103 4462 4621 518
Size: 648 1371 283 50 1233 359 159 2042
Fragments arranged by size:
2042 1371 1233 648 359 283 159 50
MspA1I CmG'CkG
Cuts at: 1606 2714 3233 3561 4238 5386 5631 1606
Size: 1108 519 328 677 1148 245 2120
Fragments arranged by size:
2120 1148 1108 677 519 328 245
NarI GG'CG_CC
Cuts at: 3036 3036
Size: 6145
NcoI C'CATG_G
Cuts at: 513 1065 2370 2558 513
Size: 552 1305 188 4100
Fragments arranged by size:
4100 1305 552 188
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6145
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6145
NotI GC'GGCC_GC
Cuts at: 3592 3592
Size: 6145
NspI r_CATG'y
Cuts at: 1132 1229 3503 5048 1132
Size: 97 2274 1545 2229
Fragments arranged by size:
2274 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6145
PciI A'CATG_T
Cuts at: 5044 5044
Size: 6145
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6145
PmeI GTTT'AAAC
Cuts at: 3449 3449
Size: 6145
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4214 4246 5727 5759 4214
Size: 32 1481 32 4600
Fragments arranged by size:
4600 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2969 2969
Size: 6145
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6145
PsiI TTA'TAA
Cuts at: 2129 3715 2129
Size: 1586 4559
PstI C_TGCA'G
Cuts at: 830 2857 3497 830
Size: 2027 640 3478
Fragments arranged by size:
3478 2027 640
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6145
PvuII CAG'CTG
Cuts at: 2714 3233 2714
Size: 519 5626
SalI G'TCGA_C
Cuts at: 1936 3487 1936
Size: 1551 4594
SanDI GG'GwC_CC
Cuts at: 2969 2969
Size: 6145
SbfI CC_TGCA'GG
Cuts at: 3497 3497
Size: 6145
ScaI AGT'ACT
Cuts at: 3398 4393 6105 3398
Size: 995 1712 3438
Fragments arranged by size:
3438 1712 995
SfcI C'TryA_G
Cuts at: 826 1045 2493 2853 3493 4628 5309 5500 826
Size: 219 1448 360 640 1135 681 191 1471
Fragments arranged by size:
1471 1448 1135 681 640 360 219 191
SfoI GGC'GCC
Cuts at: 3037 3037
Size: 6145
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6145
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6145
SmaI CCC'GGG
Cuts at: 1872 3472 1872
Size: 1600 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4249 5150 5412 820
Size: 197 926 2306 901 262 1553
Fragments arranged by size:
2306 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3936 493
Size: 3443 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6145
SphI G_CATG'C
Cuts at: 1132 3503 1132
Size: 2371 3774
SspI AAT'ATT
Cuts at: 5 52 2770 4069 5
Size: 47 2718 1299 2081
Fragments arranged by size:
2718 2081 1299 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6145
StyI C'CwwG_G
Cuts at: 513 1065 2370 2558 3132 3584 513
Size: 552 1305 188 574 452 3074
Fragments arranged by size:
3074 1305 574 552 452 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4172 4189 4342 4527 4972 325
Size: 1255 2592 17 153 185 445 1498
Fragments arranged by size:
2592 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2112 2991 3396
Size: 274 80 33 51 1443 135 879 405
Cuts at: 3396 4391 6103 96
Size: 995 1712 138
Fragments arranged by size:
1712 1443 995 879 405 274 138 135
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2184 2216 6105 6137 2184
Size: 32 3889 32 2192
Fragments arranged by size:
3889 2192 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6145
XbaI T'CTAG_A
Cuts at: 3481 3481
Size: 6145
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2711 3335 2711
Size: 624 5521
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6145
XmaI C'CCGG_G
Cuts at: 1870 3470 1870
Size: 1600 4545
XmnI GAAnn'nnTTC
Cuts at: 4274 4274
Size: 6145
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2315 3953 276
Size: 53 83 186 936 781 1638 2468
Fragments arranged by size:
2468 1638 936 781 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI
SexAI SfiI SrfI SwaI