Restriction map for
pFN21AE6410
(Circular) (Six-base) MAPSORT of: pFN21AE6410.seq Check: 5073 from: 1 to: 6109
pFN21AE6410 6109 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2070 2350 2070
Size: 280 5829
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3919 278
Size: 53 83 186 936 2383 2468
Fragments arranged by size:
2468 2383 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3452 1937
Size: 1515 4594
Acc65I G'GTAC_C
Cuts at: 3430 3430
Size: 6109
AclI AA'CG_TT
Cuts at: 1563 4236 4609 1563
Size: 2673 373 3063
Fragments arranged by size:
3063 2673 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2987 4172 5556 5824 1632
Size: 1355 1185 1384 268 1917
Fragments arranged by size:
1917 1384 1355 1185 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5912
AflIII A'CryG_T
Cuts at: 1203 5008 5985 1203
Size: 3805 977 1327
Fragments arranged by size:
3805 1327 977
AgeI A'CCGG_T
Cuts at: 4940 4940
Size: 6109
AhdI GACnn_n'nnGTC
Cuts at: 3137 4838 3137
Size: 1701 4408
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6109
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6109
AlwNI CAG_nnn'CTG
Cuts at: 3852 5424 3852
Size: 1572 4537
ApaLI G'TGCA_C
Cuts at: 4166 5322 4166
Size: 1156 4953
ApoI r'AATT_y
Cuts at: 1493 2867 3418 3644 3796 5905 1493
Size: 1374 551 226 152 2109 1697
Fragments arranged by size:
2109 1697 1374 551 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4663 160
Size: 872 3631 1606
Fragments arranged by size:
3631 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6109
AvaI C'yCGr_G
Cuts at: 1870 1943 3081 3434 1870
Size: 73 1138 353 4545
Fragments arranged by size:
4545 1138 353 73
AvrII C'CTAG_G
Cuts at: 2581 2581
Size: 6109
BamHI G'GATC_C
Cuts at: 3439 3806 3439
Size: 367 5742
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2090 2309 2936 3430 4885
Size: 325 217 627 303 219 627 494 1455
Cuts at: 4885 618
Size: 1842
Fragments arranged by size:
1842 1455 627 627 494 325 303 219
217
BanII G_rGCy'C
Cuts at: 1391 3428 1391
Size: 2037 4072
BbeI G_GCGC'C
Cuts at: 2094 2094
Size: 6109
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5138
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4300 4334 1903
Size: 34 2363 34 3678
Fragments arranged by size:
3678 2363 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4001 5211 4001
Size: 1210 4899
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6109
BfrBI ATG'CAT
Cuts at: 2452 2452
Size: 6109
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2142 4720
Size: 107 122 71 631 605 337 133 2578
Cuts at: 4720 136
Size: 1525
Fragments arranged by size:
2578 1525 631 605 337 133 122 107
71
BglII A'GATC_T
Cuts at: 6104 6104
Size: 6109
BlpI GC'TnA_GC
Cuts at: 3526 3526
Size: 6109
Bme1580I G_kGCm'C
Cuts at: 1790 4170 5326 1790
Size: 2380 1156 2573
Fragments arranged by size:
2573 2380 1156
BmgBI CAC'GTC
Cuts at: 1318 2116 2823 3342 1318
Size: 798 707 519 4085
Fragments arranged by size:
4085 798 707 519
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3840 4798 442
Size: 416 53 83 399 24 2423 958 1753
Fragments arranged by size:
2423 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6109
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2252 2542 3148 4769 1239
Size: 1013 290 606 1621 2579
Fragments arranged by size:
2579 1621 1013 606 290
Bpu10I CC'TnA_GC
Cuts at: 3335 3335
Size: 6109
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2821 4234 5099 5397 2821
Size: 1413 865 298 3533
Fragments arranged by size:
3533 1413 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2986 4772 882
Size: 617 1487 1786 2219
Fragments arranged by size:
2219 1786 1487 617
BsaAI yAC'GTr
Cuts at: 493 1204 3900 493
Size: 711 2696 2702
Fragments arranged by size:
2702 2696 711
BsaBI GATnn'nnATC
Cuts at: 6103 6103
Size: 6109
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2091 2370 3916 4298
Size: 53 83 186 936 558 279 1546 382
Cuts at: 4298 4933 275
Size: 635 1451
Fragments arranged by size:
1546 1451 936 635 558 382 279 186
83 53
BsaWI w'CCGG_w
Cuts at: 2639 4542 4940 5214 5361 5772 2639
Size: 1903 398 274 147 411 2976
Fragments arranged by size:
2976 1903 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2124 2154 2225 2255 2465
Size: 30 165 30 721 30 71 30 210
Cuts at: 2465 2495 1178
Size: 30 4792
Fragments arranged by size:
4792 721 210 165 71 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6109
BseYI C'CCAG_C
Cuts at: 5312 5808 5312
Size: 496 5613
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2064 2643 1163
Size: 573 328 579 4629
Fragments arranged by size:
4629 579 573 328
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3559 4320 5348 1540
Size: 275 186 1558 761 1028 2301
Fragments arranged by size:
2301 1558 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4170 4255 5326 4170
Size: 85 1071 4953
Fragments arranged by size:
4953 1071 85
BsmI GAATG_Cn'
Cuts at: 2361 3620 3713 2361
Size: 1259 93 4757
Fragments arranged by size:
4757 1259 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3428 4170 4255 5326 1391
Size: 399 1638 742 85 1071 2174
Fragments arranged by size:
2174 1638 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 2639 5772 2639
Size: 3133 2976
BspHI T'CATG_A
Cuts at: 2185 3998 5728 2185
Size: 1813 1730 2566
Fragments arranged by size:
2566 1813 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2070 2204 2350 3464 844
Size: 1226 134 146 1114 3489
Fragments arranged by size:
3489 1226 1114 146 134
BsrBI CCG'CTC
Cuts at: 1394 1823 2788 3996 1394
Size: 429 965 1208 3507
Fragments arranged by size:
3507 1208 965 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4604 4778 65
Size: 1183 3356 174 1396
Fragments arranged by size:
3356 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4753 4940 1700
Size: 110 2943 187 2869
Fragments arranged by size:
2943 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6109
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6109
BssSI C'ACGA_G
Cuts at: 4169 5181 4169
Size: 1012 5097
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6109
BstBI TT'CG_AA
Cuts at: 4913 4913
Size: 6109
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6109
BstYI r'GATC_y
Cuts at: 1972 3439 3806 4191 4208 5660 5746 5758 6104
Size: 1467 367 385 17 1452 86 12 346
Cuts at: 6104 1972
Size: 1977
Fragments arranged by size:
1977 1467 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2151 513
Size: 552 939 147 4471
Fragments arranged by size:
4471 939 552 147
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5370
Fragments arranged by size:
5370 683 56
BtsI GCAGTG_nn'
Cuts at: 3264 3372 3625 4419 4439 3264
Size: 108 253 794 20 4934
Fragments arranged by size:
4934 794 253 108 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6109
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6074
DraI TTT'AAA
Cuts at: 3413 3413
Size: 6109
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5116 809
Size: 507 3800 1802
Fragments arranged by size:
3800 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2550 3556 4445
Size: 54 1425 50 275 18 720 1006 889
Cuts at: 4445 6074 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1006 889 720 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3556 1537
Size: 275 1744 4090
Fragments arranged by size:
4090 1744 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3276 4039 1353
Size: 1923 763 3423
Fragments arranged by size:
3423 1923 763
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4696 5070 5216 387
Size: 1064 138 3107 374 146 1280
Fragments arranged by size:
3107 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2252 2542 2987 3148 4172 4769 5556
Size: 393 620 290 445 161 1024 597 787
Cuts at: 5556 5824 1239
Size: 268 1524
Fragments arranged by size:
1524 1024 787 620 597 445 393 290
268 161
EcoNI CCTnn'n_nnAGG
Cuts at: 2077 2077
Size: 6109
EcoO109I rG'GnC_Cy
Cuts at: 2383 2383
Size: 6109
EcoRI G'AATT_C
Cuts at: 3418 3796 3418
Size: 378 5731
EcoRV GAT'ATC
Cuts at: 3160 3160
Size: 6109
FspI TGC'GCA
Cuts at: 4615 4615
Size: 6109
HaeII r_GCGC'y
Cuts at: 1678 2094 2148 3092 5256 1678
Size: 416 54 944 2164 2531
Fragments arranged by size:
2531 2164 944 416 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2225 2257 2360 2392 2465
Size: 32 135 32 653 32 103 32 73
Cuts at: 2465 2497 3123 3155 4750 4782 4824 4856 1373
Size: 32 626 32 1595 32 42 32 2626
Fragments arranged by size:
2626 1595 653 626 135 103 73 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2884 3142 3453 3699 669
Size: 988 281 946 258 311 246 3079
Fragments arranged by size:
3079 988 946 311 281 258 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6109
HpaI GTT'AAC
Cuts at: 2884 3699 2884
Size: 815 5294
KasI G'GCGC_C
Cuts at: 2090 2090
Size: 6109
KpnI G_GTAC'C
Cuts at: 3434 3434
Size: 6109
MfeI C'AATT_G
Cuts at: 3708 3708
Size: 6109
MluI A'CGCG_T
Cuts at: 5985 5985
Size: 6109
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2451 2638 5223 5407 1305
Size: 1146 187 2585 184 2007
Fragments arranged by size:
2585 2007 1146 187 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4287
Fragments arranged by size:
4287 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2184 2832 4067 4426 4585 518
Size: 648 1018 648 1235 359 159 2042
Fragments arranged by size:
2042 1235 1018 648 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2058 2065 2933 2995 3525 4202 5350 5595
Size: 452 7 868 62 530 677 1148 245
Cuts at: 5595 1606
Size: 2120
Fragments arranged by size:
2120 1148 868 677 530 452 245 62
7
NarI GG'CG_CC
Cuts at: 2091 2091
Size: 6109
NcoI C'CATG_G
Cuts at: 513 1065 2004 2151 513
Size: 552 939 147 4471
Fragments arranged by size:
4471 939 552 147
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6109
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6109
NotI GC'GGCC_GC
Cuts at: 3556 3556
Size: 6109
NsiI A_TGCA'T
Cuts at: 2454 2454
Size: 6109
NspI r_CATG'y
Cuts at: 1132 1229 2456 2837 3062 3467 5012 1132
Size: 97 1227 381 225 405 1545 2229
Fragments arranged by size:
2229 1545 1227 405 381 225 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6109
PciI A'CATG_T
Cuts at: 5008 5008
Size: 6109
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2565 1108
Size: 1457 4652
PmeI GTTT'AAAC
Cuts at: 3413 3413
Size: 6109
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2481 2513 4178 4210 5691 5723 2481
Size: 32 1665 32 1481 32 2867
Fragments arranged by size:
2867 1665 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6109
PsiI TTA'TAA
Cuts at: 3679 3679
Size: 6109
PstI C_TGCA'G
Cuts at: 830 2949 3461 830
Size: 2119 512 3478
Fragments arranged by size:
3478 2119 512
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6109
PvuII CAG'CTG
Cuts at: 2933 2995 2933
Size: 62 6047
SalI G'TCGA_C
Cuts at: 1936 3451 1936
Size: 1515 4594
SapI GCTCTTCn'nnn_
Cuts at: 3276 3276
Size: 6109
SbfI CC_TGCA'GG
Cuts at: 3461 3461
Size: 6109
ScaI AGT'ACT
Cuts at: 2229 4357 6069 2229
Size: 2128 1712 2269
Fragments arranged by size:
2269 2128 1712
SfcI C'TryA_G
Cuts at: 826 1045 2503 2945 3282 3457 4592 5273 5464
Size: 219 1458 442 337 175 1135 681 191
Cuts at: 5464 826
Size: 1471
Fragments arranged by size:
1471 1458 1135 681 442 337 219 191
175
SfoI GGC'GCC
Cuts at: 2092 2092
Size: 6109
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6109
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6109
SmaI CCC'GGG
Cuts at: 1872 3436 1872
Size: 1564 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2800 4213 5114 5376 820
Size: 197 926 857 1413 901 262 1553
Fragments arranged by size:
1553 1413 926 901 857 262 197
SnaBI TAC'GTA
Cuts at: 493 3900 493
Size: 3407 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6109
SphI G_CATG'C
Cuts at: 1132 2456 3467 1132
Size: 1324 1011 3774
Fragments arranged by size:
3774 1324 1011
SspI AAT'ATT
Cuts at: 5 52 4033 5
Size: 47 3981 2081
Fragments arranged by size:
3981 2081 47
StuI AGG'CCT
Cuts at: 1517 2136 3079 1517
Size: 619 943 4547
Fragments arranged by size:
4547 943 619
StyI C'CwwG_G
Cuts at: 513 1065 2004 2151 2163 2564 2581 2808 3548
Size: 552 939 147 12 401 17 227 740
Cuts at: 3548 513
Size: 3074
Fragments arranged by size:
3074 939 740 552 401 227 147 17
12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2502 4136 4153 4306 4491 4936 325
Size: 1255 922 1634 17 153 185 445 1498
Fragments arranged by size:
1634 1498 1255 922 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2227 4355 6067
Size: 274 80 33 51 1443 250 2128 1712
Cuts at: 6067 96
Size: 138
Fragments arranged by size:
2128 1712 1443 274 250 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2124 2156 6069 6101 2124
Size: 32 3913 32 2132
Fragments arranged by size:
3913 2132 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3255 1316
Size: 1939 4170
XbaI T'CTAG_A
Cuts at: 3445 3445
Size: 6109
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6109
XmaI C'CCGG_G
Cuts at: 1870 3434 1870
Size: 1564 4545
XmnI GAAnn'nnTTC
Cuts at: 4238 4238
Size: 6109
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3917 276
Size: 53 83 186 936 2383 2468
Fragments arranged by size:
2468 2383 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbvCI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI FalI FseI
FspAI NaeI NgoMIV NruI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI
SfiI SrfI SwaI XcmI