Restriction map for
pFN21AE6409
(Circular) (Six-base) MAPSORT of: pFN21AE6409.seq Check: 2416 from: 1 to: 6118
pFN21AE6409 6118 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3928 278
Size: 53 83 186 936 2392 2468
Fragments arranged by size:
2468 2392 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2878 3461 1937
Size: 941 583 4594
Fragments arranged by size:
4594 941 583
Acc65I G'GTAC_C
Cuts at: 3439 3439
Size: 6118
AclI AA'CG_TT
Cuts at: 1563 4245 4618 1563
Size: 2682 373 3063
Fragments arranged by size:
3063 2682 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2078 2127 2250 2495 2700 4181 5565 5833
Size: 446 49 123 245 205 1481 1384 268
Cuts at: 5833 1632
Size: 1917
Fragments arranged by size:
1917 1481 1384 446 268 245 205 123
49
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5921
AflIII A'CryG_T
Cuts at: 1203 5017 5994 1203
Size: 3814 977 1327
Fragments arranged by size:
3814 1327 977
AgeI A'CCGG_T
Cuts at: 4949 4949
Size: 6118
AhdI GACnn_n'nnGTC
Cuts at: 4847 4847
Size: 6118
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6118
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2759 1184
Size: 1575 4543
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2690 2722 2690
Size: 32 6086
AlwNI CAG_nnn'CTG
Cuts at: 3861 5433 3861
Size: 1572 4546
ApaLI G'TGCA_C
Cuts at: 4175 5331 4175
Size: 1156 4962
ApoI r'AATT_y
Cuts at: 1493 2294 3427 3653 3805 5914 1493
Size: 801 1133 226 152 2109 1697
Fragments arranged by size:
2109 1697 1133 801 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4672 160
Size: 872 3640 1606
Fragments arranged by size:
3640 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6118
AvaI C'yCGr_G
Cuts at: 1870 1943 3443 1870
Size: 73 1500 4545
Fragments arranged by size:
4545 1500 73
AvrII C'CTAG_G
Cuts at: 3127 3127
Size: 6118
BamHI G'GATC_C
Cuts at: 3448 3815 3448
Size: 367 5751
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2654 3439 4894 618
Size: 325 217 627 867 785 1455 1842
Fragments arranged by size:
1842 1455 867 785 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2088 2922 3321 3437 1391
Size: 697 834 399 116 4072
Fragments arranged by size:
4072 834 697 399 116
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5147
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4309 4343 1903
Size: 34 2372 34 3678
Fragments arranged by size:
3678 2372 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4010 5220 4010
Size: 1210 4908
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6118
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4729 136
Size: 107 122 71 631 605 3057 1525
Fragments arranged by size:
3057 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6113 6113
Size: 6118
BlpI GC'TnA_GC
Cuts at: 3535 3535
Size: 6118
Bme1580I G_kGCm'C
Cuts at: 1790 4179 5335 1790
Size: 2389 1156 2573
Fragments arranged by size:
2573 2389 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6118
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3849 4807 442
Size: 416 53 83 399 24 2432 958 1753
Fragments arranged by size:
2432 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6118
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4778 1239
Size: 3539 2579
Bpu10I CC'TnA_GC
Cuts at: 2628 2628
Size: 6118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2102 2837 4243 5108 5406 2102
Size: 735 1406 865 298 2814
Fragments arranged by size:
2814 1406 865 735 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3058 3119 4781 882
Size: 617 1559 61 1662 2219
Fragments arranged by size:
2219 1662 1559 617 61
BsaAI yAC'GTr
Cuts at: 493 1204 3909 493
Size: 711 2705 2702
Fragments arranged by size:
2705 2702 711
BsaBI GATnn'nnATC
Cuts at: 2688 6112 2688
Size: 3424 2694
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3925 4307 4942 275
Size: 53 83 186 936 2392 382 635 1451
Fragments arranged by size:
2392 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4551 4949 5223 5370 5781 4551
Size: 398 274 147 411 4888
Fragments arranged by size:
4888 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2022 2052 1178
Size: 30 165 30 619 30 5244
Fragments arranged by size:
5244 619 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2133 2975 1186
Size: 947 842 4329
Fragments arranged by size:
4329 947 842
BseYI C'CCAG_C
Cuts at: 2033 5321 5817 2033
Size: 3288 496 2334
Fragments arranged by size:
3288 2334 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5545
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3568 4329 5357 1540
Size: 275 186 1567 761 1028 2301
Fragments arranged by size:
2301 1567 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2922 3321 4179 4264 5335 2922
Size: 399 858 85 1071 3705
Fragments arranged by size:
3705 1071 858 399 85
BsmI GAATG_Cn'
Cuts at: 2626 3629 3722 2626
Size: 1003 93 5022
Fragments arranged by size:
5022 1003 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2088 2922 3321 3437 4179 4264 5335
Size: 399 298 834 399 116 742 85 1071
Cuts at: 5335 1391
Size: 2174
Fragments arranged by size:
2174 1071 834 742 399 399 298 116
85
BspEI T'CCGG_A
Cuts at: 5781 5781
Size: 6118
BspHI T'CATG_A
Cuts at: 2664 4007 5737 2664
Size: 1343 1730 3045
Fragments arranged by size:
3045 1730 1343
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3473 844
Size: 2629 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3080 4005 1394
Size: 429 1257 925 3507
Fragments arranged by size:
3507 1257 925 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2743 4613 4787 65
Size: 1183 1495 1870 174 1396
Fragments arranged by size:
1870 1495 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4762 4949 1700
Size: 110 2952 187 2869
Fragments arranged by size:
2952 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6118
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6118
BssSI C'ACGA_G
Cuts at: 4178 5190 4178
Size: 1012 5106
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6118
BstBI TT'CG_AA
Cuts at: 4922 4922
Size: 6118
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6118
BstYI r'GATC_y
Cuts at: 1972 3370 3448 3815 4200 4217 5669 5755 5767
Size: 1398 78 367 385 17 1452 86 12
Cuts at: 5767 6113 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1398 385 367 346 86 78
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4627
Fragments arranged by size:
4627 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5379
Fragments arranged by size:
5379 683 56
BtsI GCAGTG_nn'
Cuts at: 2449 3634 4428 4448 2449
Size: 1185 794 20 4119
Fragments arranged by size:
4119 1185 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6118
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2077 2112 529
Size: 35 1513 35 4535
Fragments arranged by size:
4535 1513 35 35
DraI TTT'AAA
Cuts at: 3422 3422
Size: 6118
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5125 809
Size: 507 3809 1802
Fragments arranged by size:
3809 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2152 2371 2446
Size: 54 1425 50 275 18 322 219 75
Cuts at: 2446 3565 4454 6083 8
Size: 1119 889 1629 43
Fragments arranged by size:
1629 1425 1119 889 322 275 219 75
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3565 1537
Size: 275 1753 4090
Fragments arranged by size:
4090 1753 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4048 1353
Size: 2695 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4705 5079 5225 387
Size: 1064 138 3116 374 146 1280
Fragments arranged by size:
3116 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2920 3319 2920
Size: 399 5719
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2078 2127 2250 2495 2700 4181 4778
Size: 393 446 49 123 245 205 1481 597
Cuts at: 4778 5565 5833 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1481 787 597 446 393 268 245
205 123 49
EcoRI G'AATT_C
Cuts at: 3427 3805 3427
Size: 378 5740
FspI TGC'GCA
Cuts at: 4624 4624
Size: 6118
HaeII r_GCGC'y
Cuts at: 1678 5265 1678
Size: 3587 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2022 2054 2148 2180 2335
Size: 32 135 32 450 32 94 32 155
Cuts at: 2335 2348 2367 2380 3338 3370 4759 4791 4833
Size: 13 19 13 958 32 1389 32 42
Cuts at: 4833 4865 1373
Size: 32 2626
Fragments arranged by size:
2626 1389 958 450 155 135 94 42
32 32 32 32 32 32 32 19
13 13
HincII GTy'rAC
Cuts at: 669 1657 1938 3096 3304 3462 3708 669
Size: 988 281 1158 208 158 246 3079
Fragments arranged by size:
3079 1158 988 281 246 208 158
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6118
HpaI GTT'AAC
Cuts at: 3708 3708
Size: 6118
KpnI G_GTAC'C
Cuts at: 3443 3443
Size: 6118
MfeI C'AATT_G
Cuts at: 3717 3717
Size: 6118
MluI A'CGCG_T
Cuts at: 5994 5994
Size: 6118
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5232 5416 1305
Size: 3927 184 2007
Fragments arranged by size:
3927 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2373 2448 10
Size: 54 1425 343 541 75 3680
Fragments arranged by size:
3680 1425 541 343 75 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2541 2567 2897 4076 4435 4594 518
Size: 648 1375 26 330 1179 359 159 2042
Fragments arranged by size:
2042 1375 1179 648 359 330 159 26
MspA1I CmG'CkG
Cuts at: 1606 2756 3534 4211 5359 5604 1606
Size: 1150 778 677 1148 245 2120
Fragments arranged by size:
2120 1150 1148 778 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4627
Fragments arranged by size:
4627 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6118
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6118
NotI GC'GGCC_GC
Cuts at: 3565 3565
Size: 6118
NspI r_CATG'y
Cuts at: 1132 1229 3476 5021 1132
Size: 97 2247 1545 2229
Fragments arranged by size:
2247 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6118
PciI A'CATG_T
Cuts at: 5017 5017
Size: 6118
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6118
PmeI GTTT'AAAC
Cuts at: 3422 3422
Size: 6118
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4187 4219 5700 5732 4187
Size: 32 1481 32 4573
Fragments arranged by size:
4573 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2330 1613
Size: 717 5401
PsiI TTA'TAA
Cuts at: 3688 3688
Size: 6118
PstI C_TGCA'G
Cuts at: 830 3470 830
Size: 2640 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6118
PvuII CAG'CTG
Cuts at: 2756 2756
Size: 6118
SacI G_AGCT'C
Cuts at: 2922 3321 2922
Size: 399 5719
SalI G'TCGA_C
Cuts at: 1936 3460 1936
Size: 1524 4594
SbfI CC_TGCA'GG
Cuts at: 3470 3470
Size: 6118
ScaI AGT'ACT
Cuts at: 4366 6078 4366
Size: 1712 4406
SfcI C'TryA_G
Cuts at: 826 1045 2907 3466 4601 5282 5473 826
Size: 219 1862 559 1135 681 191 1471
Fragments arranged by size:
1862 1471 1135 681 559 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6118
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6118
SmaI CCC'GGG
Cuts at: 1872 3445 1872
Size: 1573 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2081 2816 4222 5123 5385 820
Size: 197 926 138 735 1406 901 262 1553
Fragments arranged by size:
1553 1406 926 901 735 262 197 138
SnaBI TAC'GTA
Cuts at: 493 3909 493
Size: 3416 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6118
SphI G_CATG'C
Cuts at: 1132 3476 1132
Size: 2344 3774
SspI AAT'ATT
Cuts at: 5 52 2273 2513 4042 5
Size: 47 2221 240 1529 2081
Fragments arranged by size:
2221 2081 1529 240 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6118
StyI C'CwwG_G
Cuts at: 513 1065 2004 2693 3127 3557 513
Size: 552 939 689 434 430 3074
Fragments arranged by size:
3074 939 689 552 434 430
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3157 4145 4162 4315 4500 4945 325
Size: 1255 1577 988 17 153 185 445 1498
Fragments arranged by size:
1577 1498 1255 988 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2740 2851 4364
Size: 274 80 33 51 1443 763 111 1513
Cuts at: 4364 6076 96
Size: 1712 138
Fragments arranged by size:
1712 1513 1443 763 274 138 111 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6078 6110 6078
Size: 32 6086
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6118
XbaI T'CTAG_A
Cuts at: 3454 3454
Size: 6118
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2807 2807
Size: 6118
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6118
XmaI C'CCGG_G
Cuts at: 1870 3443 1870
Size: 1573 4545
XmnI GAAnn'nnTTC
Cuts at: 2235 4247 2235
Size: 2012 4106
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3926 276
Size: 53 83 186 936 2392 2468
Fragments arranged by size:
2468 2392 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI