Restriction map for
pFN21AE6408
(Circular) (Six-base) MAPSORT of: pFN21AE6408.seq Check: 4723 from: 1 to: 6304
pFN21AE6408 6304 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2566 2566
Size: 6304
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4114 278
Size: 53 83 186 936 2578 2468
Fragments arranged by size:
2578 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2917 3647 1937
Size: 980 730 4594
Fragments arranged by size:
4594 980 730
Acc65I G'GTAC_C
Cuts at: 2940 3625 2940
Size: 685 5619
AclI AA'CG_TT
Cuts at: 1563 2786 4431 4804 1563
Size: 1223 1645 373 3063
Fragments arranged by size:
3063 1645 1223 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2405 4367 5751 6019 1632
Size: 773 1962 1384 268 1917
Fragments arranged by size:
1962 1917 1384 773 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6107
AflIII A'CryG_T
Cuts at: 1203 5203 6180 1203
Size: 4000 977 1327
Fragments arranged by size:
4000 1327 977
AgeI A'CCGG_T
Cuts at: 5135 5135
Size: 6304
AhdI GACnn_n'nnGTC
Cuts at: 5033 5033
Size: 6304
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6304
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2610 1184
Size: 1426 4878
AlwNI CAG_nnn'CTG
Cuts at: 2134 2225 2632 2807 4047 5619 2134
Size: 91 407 175 1240 1572 2819
Fragments arranged by size:
2819 1572 1240 407 175 91
ApaI G_GGCC'C
Cuts at: 2962 2962
Size: 6304
ApaLI G'TGCA_C
Cuts at: 4361 5517 4361
Size: 1156 5148
ApoI r'AATT_y
Cuts at: 1493 2069 3613 3839 3991 6100 1493
Size: 576 1544 226 152 2109 1697
Fragments arranged by size:
2109 1697 1544 576 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4858 160
Size: 872 3826 1606
Fragments arranged by size:
3826 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6304
AvaI C'yCGr_G
Cuts at: 1870 1943 3192 3629 1870
Size: 73 1249 437 4545
Fragments arranged by size:
4545 1249 437 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3432 3465 3432
Size: 33 6271
BamHI G'GATC_C
Cuts at: 3634 4001 3634
Size: 367 5937
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2940 3392 3625 5080 618
Size: 325 217 627 1153 452 233 1455 1842
Fragments arranged by size:
1842 1455 1153 627 452 325 233 217
BanII G_rGCy'C
Cuts at: 1391 2691 2962 3623 1391
Size: 1300 271 661 4072
Fragments arranged by size:
4072 1300 661 271
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2647 3394 928
Size: 971 748 747 3838
Fragments arranged by size:
3838 971 748 747
BbvCI CC'TCA_GC
Cuts at: 2232 2642 2232
Size: 410 5894
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4495 4529 1903
Size: 34 2558 34 3678
Fragments arranged by size:
3678 2558 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4196 5406 4196
Size: 1210 5094
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6304
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4915 136
Size: 107 122 71 631 605 3243 1525
Fragments arranged by size:
3243 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6299 6299
Size: 6304
BlpI GC'TnA_GC
Cuts at: 2808 3721 2808
Size: 913 5391
Bme1580I G_kGCm'C
Cuts at: 1790 2091 2683 2962 4365 5521 1790
Size: 301 592 279 1403 1156 2573
Fragments arranged by size:
2573 1403 1156 592 301 279
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6304
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4035 4993 442
Size: 416 53 83 399 24 2618 958 1753
Fragments arranged by size:
2618 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2816 1056
Size: 1760 4544
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2324 2705 2754 4964 1239
Size: 1085 381 49 2210 2579
Fragments arranged by size:
2579 2210 1085 381 49
Bpu10I CC'TnA_GC
Cuts at: 2051 2232 2642 2051
Size: 181 410 5713
Fragments arranged by size:
5713 410 181
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3595 4429 5294 5592 3595
Size: 834 865 298 4307
Fragments arranged by size:
4307 865 834 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3541 3571 4967 882
Size: 617 2042 30 1396 2219
Fragments arranged by size:
2219 2042 1396 617 30
BsaAI yAC'GTr
Cuts at: 493 1204 4095 493
Size: 711 2891 2702
Fragments arranged by size:
2891 2702 711
BsaBI GATnn'nnATC
Cuts at: 6298 6298
Size: 6304
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4111 4493 5128 275
Size: 53 83 186 936 2578 382 635 1451
Fragments arranged by size:
2578 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4737 5135 5409 5556 5967 4737
Size: 398 274 147 411 5074
Fragments arranged by size:
5074 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2984 3014 1178
Size: 30 165 30 1581 30 4468
Fragments arranged by size:
4468 1581 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2618 3005 1186
Size: 1432 387 4485
Fragments arranged by size:
4485 1432 387
BseYI C'CCAG_C
Cuts at: 2184 5507 6003 2184
Size: 3323 496 2485
Fragments arranged by size:
3323 2485 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2081 2147 2378 2657 1163
Size: 573 345 66 231 279 4810
Fragments arranged by size:
4810 573 345 279 231 66
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3754 4515 5543 1540
Size: 275 186 1753 761 1028 2301
Fragments arranged by size:
2301 1753 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2691 4365 4450 5521 2691
Size: 1674 85 1071 3474
Fragments arranged by size:
3474 1674 1071 85
BsmI GAATG_Cn'
Cuts at: 2263 3815 3908 2263
Size: 1552 93 4659
Fragments arranged by size:
4659 1552 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2091 2683 2691 2962 3623 4365 4450
Size: 399 301 592 8 271 661 742 85
Cuts at: 4450 5521 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 661 592 399 301 271
85 8
BspEI T'CCGG_A
Cuts at: 5967 5967
Size: 6304
BspHI T'CATG_A
Cuts at: 4193 5923 4193
Size: 1730 4574
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2324 2566 2862 3659 844
Size: 1480 242 296 797 3489
Fragments arranged by size:
3489 1480 797 296 242
BsrBI CCG'CTC
Cuts at: 1394 1823 2526 3474 4191 1394
Size: 429 703 948 717 3507
Fragments arranged by size:
3507 948 717 703 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2024 2496 2973 4799 4973 65
Size: 1183 776 472 477 1826 174 1396
Fragments arranged by size:
1826 1396 1183 776 477 472 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4948 5135 1700
Size: 110 3138 187 2869
Fragments arranged by size:
3138 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6304
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6304
BssSI C'ACGA_G
Cuts at: 3090 4364 5376 3090
Size: 1274 1012 4018
Fragments arranged by size:
4018 1274 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6304
BstBI TT'CG_AA
Cuts at: 5108 5108
Size: 6304
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6304
BstXI CCAn_nnnn'nTGG
Cuts at: 2197 2197
Size: 6304
BstYI r'GATC_y
Cuts at: 1972 3634 4001 4386 4403 5855 5941 5953 6299
Size: 1662 367 385 17 1452 86 12 346
Cuts at: 6299 1972
Size: 1977
Fragments arranged by size:
1977 1662 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2947 513
Size: 552 1882 3870
Fragments arranged by size:
3870 1882 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5565
Fragments arranged by size:
5565 683 56
BtsI GCAGTG_nn'
Cuts at: 3820 4614 4634 3820
Size: 794 20 5490
Fragments arranged by size:
5490 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6304
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6269
DraI TTT'AAA
Cuts at: 3608 3608
Size: 6304
DraIII CAC_nnn'GTG
Cuts at: 2254 2254
Size: 6304
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2215 5311 809
Size: 507 899 3096 1802
Fragments arranged by size:
3096 1802 899 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3751 4640 6269
Size: 54 1425 50 275 18 1921 889 1629
Cuts at: 6269 8
Size: 43
Fragments arranged by size:
1921 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3751 1537
Size: 275 1939 4090
Fragments arranged by size:
4090 1939 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2766 2918 4234 1353
Size: 1413 152 1316 3423
Fragments arranged by size:
3423 1413 1316 152
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4891 5265 5411 387
Size: 1064 138 3302 374 146 1280
Fragments arranged by size:
3302 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2689 2689
Size: 6304
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2324 2405 2705 2754 4367 4964 5751
Size: 393 692 81 300 49 1613 597 787
Cuts at: 5751 6019 1239
Size: 268 1524
Fragments arranged by size:
1613 1524 787 692 597 393 300 268
81 49
EcoNI CCTnn'n_nnAGG
Cuts at: 2153 2153
Size: 6304
EcoO109I rG'GnC_Cy
Cuts at: 2308 2958 2959 2308
Size: 650 1 5653
Fragments arranged by size:
5653 650 1
EcoRI G'AATT_C
Cuts at: 2069 3613 3991 2069
Size: 1544 378 4382
Fragments arranged by size:
4382 1544 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2183 2183
Size: 6304
FspI TGC'GCA
Cuts at: 4810 4810
Size: 6304
HaeII r_GCGC'y
Cuts at: 1678 5451 1678
Size: 3773 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2869 2901 3023 3055 4945
Size: 32 135 32 1297 32 122 32 1890
Cuts at: 4945 4977 5019 5051 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1890 1297 135 122 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2790 3228 3601 3648 3894 669
Size: 988 281 852 438 373 47 246 3079
Fragments arranged by size:
3079 988 852 438 373 281 246 47
HindIII A'AGCT_T
Cuts at: 748 2165 748
Size: 1417 4887
HpaI GTT'AAC
Cuts at: 2790 3228 3894 2790
Size: 438 666 5200
Fragments arranged by size:
5200 666 438
KpnI G_GTAC'C
Cuts at: 2944 3629 2944
Size: 685 5619
MfeI C'AATT_G
Cuts at: 3903 3903
Size: 6304
MluI A'CGCG_T
Cuts at: 6180 6180
Size: 6304
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3185 5418 5602 1305
Size: 1880 2233 184 2007
Fragments arranged by size:
2233 2007 1880 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4482
Fragments arranged by size:
4482 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2150 4262 4621 4780 518
Size: 648 984 2112 359 159 2042
Fragments arranged by size:
2112 2042 984 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2222 2330 2362 2968 3324 3446 3720 4397
Size: 616 108 32 606 356 122 274 677
Cuts at: 4397 5545 5790 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 616 606 356 274 245
122 108 32
NcoI C'CATG_G
Cuts at: 513 1065 2947 513
Size: 552 1882 3870
Fragments arranged by size:
3870 1882 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6304
NheI G'CTAG_C
Cuts at: 1052 2812 1052
Size: 1760 4544
NotI GC'GGCC_GC
Cuts at: 3751 3751
Size: 6304
NspI r_CATG'y
Cuts at: 1132 1229 3503 3662 5207 1132
Size: 97 2274 159 1545 2229
Fragments arranged by size:
2274 2229 1545 159 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6304
PciI A'CATG_T
Cuts at: 5203 5203
Size: 6304
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3546 1108
Size: 2438 3866
PfoI T'CCnGG_A
Cuts at: 2470 2470
Size: 6304
PmeI GTTT'AAAC
Cuts at: 3608 3608
Size: 6304
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3084 3116 4373 4405 5886 5918 3084
Size: 32 1257 32 1481 32 3470
Fragments arranged by size:
3470 1481 1257 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2308 2308
Size: 6304
PshAI GACnn'nnGTC
Cuts at: 1613 3119 1613
Size: 1506 4798
PsiI TTA'TAA
Cuts at: 3874 3874
Size: 6304
PspOMI G'GGCC_C
Cuts at: 2958 2958
Size: 6304
PstI C_TGCA'G
Cuts at: 830 2322 2335 3244 3656 830
Size: 1492 13 909 412 3478
Fragments arranged by size:
3478 1492 909 412 13
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6304
PvuII CAG'CTG
Cuts at: 2222 2330 2968 3446 2222
Size: 108 638 478 5080
Fragments arranged by size:
5080 638 478 108
SacI G_AGCT'C
Cuts at: 2691 2691
Size: 6304
SalI G'TCGA_C
Cuts at: 1936 3646 1936
Size: 1710 4594
SbfI CC_TGCA'GG
Cuts at: 2322 3656 2322
Size: 1334 4970
ScaI AGT'ACT
Cuts at: 4552 6264 4552
Size: 1712 4592
SfcI C'TryA_G
Cuts at: 826 1045 2318 2331 2935 3240 3652 4787 5468
Size: 219 1273 13 604 305 412 1135 681
Cuts at: 5468 5659 826
Size: 191 1471
Fragments arranged by size:
1471 1273 1135 681 604 412 305 219
191 13
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6304
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6304
SmaI CCC'GGG
Cuts at: 1872 3631 1872
Size: 1759 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3574 4408 5309 5571 820
Size: 197 926 1631 834 901 262 1553
Fragments arranged by size:
1631 1553 926 901 834 262 197
SnaBI TAC'GTA
Cuts at: 493 4095 493
Size: 3602 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6304
SphI G_CATG'C
Cuts at: 1132 3662 1132
Size: 2530 3774
SspI AAT'ATT
Cuts at: 5 52 4228 5
Size: 47 4176 2081
Fragments arranged by size:
4176 2081 47
StuI AGG'CCT
Cuts at: 1517 2842 1517
Size: 1325 4979
StyI C'CwwG_G
Cuts at: 513 1065 2947 3743 513
Size: 552 1882 796 3074
Fragments arranged by size:
3074 1882 796 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2005 2654 4331 4348 4501 4686 5131
Size: 1255 425 649 1677 17 153 185 445
Cuts at: 5131 325
Size: 1498
Fragments arranged by size:
1677 1498 1255 649 445 425 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2142 4550 6262
Size: 274 80 33 51 1443 165 2408 1712
Cuts at: 6262 96
Size: 138
Fragments arranged by size:
2408 1712 1443 274 165 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2984 3016 6264 6296 2984
Size: 32 3248 32 2992
Fragments arranged by size:
3248 2992 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2312 1316
Size: 996 5308
XbaI T'CTAG_A
Cuts at: 3640 3640
Size: 6304
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2729 2729
Size: 6304
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6304
XmaI C'CCGG_G
Cuts at: 1870 3629 1870
Size: 1759 4545
XmnI GAAnn'nnTTC
Cuts at: 4433 4433
Size: 6304
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4112 276
Size: 53 83 186 936 2578 2468
Fragments arranged by size:
2578 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI