Restriction map for
pFN21AE6407
(Circular) (Six-base) MAPSORT of: pFN21AE6407.seq Check: 5296 from: 1 to: 6193
pFN21AE6407 6193 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4003 278
Size: 53 83 186 936 2467 2468
Fragments arranged by size:
2468 2467 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3536 1937
Size: 1599 4594
Acc65I G'GTAC_C
Cuts at: 3514 3514
Size: 6193
AclI AA'CG_TT
Cuts at: 1563 4320 4693 1563
Size: 2757 373 3063
Fragments arranged by size:
3063 2757 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2709 4256 5640 5908 1632
Size: 1077 1547 1384 268 1917
Fragments arranged by size:
1917 1547 1384 1077 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5996
AflIII A'CryG_T
Cuts at: 1203 2502 5092 6069 1203
Size: 1299 2590 977 1327
Fragments arranged by size:
2590 1327 1299 977
AgeI A'CCGG_T
Cuts at: 5024 5024
Size: 6193
AhdI GACnn_n'nnGTC
Cuts at: 4922 4922
Size: 6193
AleI CACnn'nnGTG
Cuts at: 1166 2670 1166
Size: 1504 4689
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6193
AlwNI CAG_nnn'CTG
Cuts at: 2037 3936 5508 2037
Size: 1899 1572 2722
Fragments arranged by size:
2722 1899 1572
ApaLI G'TGCA_C
Cuts at: 4250 5406 4250
Size: 1156 5037
ApoI r'AATT_y
Cuts at: 1493 3357 3502 3728 3880 5989 1493
Size: 1864 145 226 152 2109 1697
Fragments arranged by size:
2109 1864 1697 226 152 145
AseI AT'TA_AT
Cuts at: 160 1032 4747 160
Size: 872 3715 1606
Fragments arranged by size:
3715 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6193
AvaI C'yCGr_G
Cuts at: 1870 1943 2402 3518 1870
Size: 73 459 1116 4545
Fragments arranged by size:
4545 1116 459 73
BamHI G'GATC_C
Cuts at: 3523 3890 3523
Size: 367 5826
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2923 3514 4969 618
Size: 325 217 627 1136 591 1455 1842
Fragments arranged by size:
1842 1455 1136 627 591 325 217
BanII G_rGCy'C
Cuts at: 1391 3297 3422 3512 1391
Size: 1906 125 90 4072
Fragments arranged by size:
4072 1906 125 90
BbeI G_GCGC'C
Cuts at: 2927 2927
Size: 6193
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2321 3049 928
Size: 971 422 728 4072
Fragments arranged by size:
4072 971 728 422
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4384 4418 1903
Size: 34 2447 34 3678
Fragments arranged by size:
3678 2447 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2500 4085 5295 2500
Size: 1585 1210 3398
Fragments arranged by size:
3398 1585 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6193
BfrBI ATG'CAT
Cuts at: 3094 3094
Size: 6193
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2366 4804 136
Size: 107 122 71 631 605 694 2438 1525
Fragments arranged by size:
2438 1525 694 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2166 6188 2166
Size: 4022 2171
BlpI GC'TnA_GC
Cuts at: 3610 3610
Size: 6193
Bme1580I G_kGCm'C
Cuts at: 1790 4254 5410 1790
Size: 2464 1156 2573
Fragments arranged by size:
2573 2464 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6193
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3034 3924 4882
Size: 416 53 83 399 24 1617 890 958
Cuts at: 4882 442
Size: 1753
Fragments arranged by size:
1753 1617 958 890 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6193
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2839 3441 2839
Size: 602 5591
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4853 1239
Size: 3614 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4318 5183 5481 4318
Size: 865 298 5030
Fragments arranged by size:
5030 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2083 2853 4856 882
Size: 617 584 770 2003 2219
Fragments arranged by size:
2219 2003 770 617 584
BsaAI yAC'GTr
Cuts at: 493 1204 3984 493
Size: 711 2780 2702
Fragments arranged by size:
2780 2702 711
BsaBI GATnn'nnATC
Cuts at: 6187 6187
Size: 6193
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2924 4000 4382 5017
Size: 53 83 186 936 1391 1076 382 635
Cuts at: 5017 275
Size: 1451
Fragments arranged by size:
1451 1391 1076 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4626 5024 5298 5445 5856 4626
Size: 398 274 147 411 4963
Fragments arranged by size:
4963 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2973 3003 1178
Size: 30 165 30 1570 30 4368
Fragments arranged by size:
4368 1570 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2815 1186
Size: 1629 4564
BseYI C'CCAG_C
Cuts at: 2612 5396 5892 2612
Size: 2784 496 2913
Fragments arranged by size:
2913 2784 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2169 2813 1163
Size: 573 433 644 4543
Fragments arranged by size:
4543 644 573 433
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3643 4404 5432 1540
Size: 275 186 1642 761 1028 2301
Fragments arranged by size:
2301 1642 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2409 4254 4339 5410 2409
Size: 1845 85 1071 3192
Fragments arranged by size:
3192 1845 1071 85
BsmI GAATG_Cn'
Cuts at: 3312 3704 3797 3312
Size: 392 93 5708
Fragments arranged by size:
5708 392 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2409 3297 3422 3512 4254 4339 5410
Size: 399 619 888 125 90 742 85 1071
Cuts at: 5410 1391
Size: 2174
Fragments arranged by size:
2174 1071 888 742 619 399 125 90
85
BspEI T'CCGG_A
Cuts at: 5856 5856
Size: 6193
BspHI T'CATG_A
Cuts at: 3264 4082 5812 3264
Size: 818 1730 3645
Fragments arranged by size:
3645 1730 818
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2314 2864 3548 844
Size: 1470 550 684 3489
Fragments arranged by size:
3489 1470 684 550
BsrBI CCG'CTC
Cuts at: 1394 1823 2424 4080 1394
Size: 429 601 1656 3507
Fragments arranged by size:
3507 1656 601 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2776 4688 4862 65
Size: 1183 1528 1912 174 1396
Fragments arranged by size:
1912 1528 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3074 4837 5024 1700
Size: 110 1264 1763 187 2869
Fragments arranged by size:
2869 1763 1264 187 110
BsrGI T'GTAC_A
Cuts at: 96 3412 96
Size: 3316 2877
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6193
BssSI C'ACGA_G
Cuts at: 4253 5265 4253
Size: 1012 5181
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3350 794
Size: 2556 3637
BstBI TT'CG_AA
Cuts at: 4997 4997
Size: 6193
BstEII G'GTnAC_C
Cuts at: 1184 2350 1184
Size: 1166 5027
BstYI r'GATC_y
Cuts at: 1972 2166 3523 3890 4275 4292 5744 5830 5842
Size: 194 1357 367 385 17 1452 86 12
Cuts at: 5842 6188 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1357 385 367 346 194 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2547 513
Size: 552 939 543 4159
Fragments arranged by size:
4159 939 552 543
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5454
Fragments arranged by size:
5454 683 56
BtsI GCAGTG_nn'
Cuts at: 2801 3009 3709 4503 4523 2801
Size: 208 700 794 20 4471
Fragments arranged by size:
4471 794 700 208 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6193
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2891 2926 529
Size: 35 2327 35 3796
Fragments arranged by size:
3796 2327 35 35
DraI TTT'AAA
Cuts at: 3497 3497
Size: 6193
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5200 809
Size: 507 3884 1802
Fragments arranged by size:
3884 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2302 2448 3640
Size: 54 1425 50 275 18 472 146 1192
Cuts at: 3640 4529 6158 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1192 889 472 275 146 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3640 1537
Size: 275 1828 4090
Fragments arranged by size:
4090 1828 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2685 3456 4123 1353
Size: 1332 771 667 3423
Fragments arranged by size:
3423 1332 771 667
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4780 5154 5300 387
Size: 1064 138 3191 374 146 1280
Fragments arranged by size:
3191 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2709 4256 4853 5640 5908 1239
Size: 393 1077 1547 597 787 268 1524
Fragments arranged by size:
1547 1524 1077 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 3028 3028
Size: 6193
EcoRI G'AATT_C
Cuts at: 3502 3880 3502
Size: 378 5815
FspI TGC'GCA
Cuts at: 4699 4699
Size: 6193
HaeII r_GCGC'y
Cuts at: 1678 2927 5340 1678
Size: 1249 2413 2531
Fragments arranged by size:
2531 2413 1249
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4834 4866 4908 4940 1373
Size: 32 135 32 3262 32 42 32 2626
Fragments arranged by size:
3262 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3022 3483 3537 3783 669
Size: 988 281 1084 461 54 246 3079
Fragments arranged by size:
3079 1084 988 461 281 246 54
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6193
HpaI GTT'AAC
Cuts at: 3783 3783
Size: 6193
KasI G'GCGC_C
Cuts at: 2923 2923
Size: 6193
KpnI G_GTAC'C
Cuts at: 3518 3518
Size: 6193
MfeI C'AATT_G
Cuts at: 3792 3792
Size: 6193
MluI A'CGCG_T
Cuts at: 6069 6069
Size: 6193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5307 5491 1305
Size: 4002 184 2007
Fragments arranged by size:
4002 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2304 10
Size: 54 1425 343 472 3899
Fragments arranged by size:
3899 1425 472 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2670 3269 4151 4510 4669 518
Size: 648 1504 599 882 359 159 2042
Fragments arranged by size:
2042 1504 882 648 599 359 159
MspA1I CmG'CkG
Cuts at: 1606 2749 3609 4286 5434 5679 1606
Size: 1143 860 677 1148 245 2120
Fragments arranged by size:
2120 1148 1143 860 677 245
NarI GG'CG_CC
Cuts at: 2924 2924
Size: 6193
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4702
Fragments arranged by size:
4702 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6193
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6193
NotI GC'GGCC_GC
Cuts at: 3640 3640
Size: 6193
NsiI A_TGCA'T
Cuts at: 3096 3096
Size: 6193
NspI r_CATG'y
Cuts at: 1132 1229 2506 2596 3379 3551 5096 1132
Size: 97 1277 90 783 172 1545 2229
Fragments arranged by size:
2229 1545 1277 783 172 97 90
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6193
PciI A'CATG_T
Cuts at: 2502 5092 2502
Size: 2590 3603
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3111 1108
Size: 2003 4190
PfoI T'CCnGG_A
Cuts at: 2036 2036
Size: 6193
PmeI GTTT'AAAC
Cuts at: 3497 3497
Size: 6193
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4262 4294 5775 5807 4262
Size: 32 1481 32 4648
Fragments arranged by size:
4648 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3028 3028
Size: 6193
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6193
PsiI TTA'TAA
Cuts at: 3164 3763 3164
Size: 599 5594
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2492 2524 2492
Size: 32 6161
PstI C_TGCA'G
Cuts at: 830 2435 3545 830
Size: 1605 1110 3478
Fragments arranged by size:
3478 1605 1110
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6193
PvuII CAG'CTG
Cuts at: 2749 2749
Size: 6193
SalI G'TCGA_C
Cuts at: 1936 3535 1936
Size: 1599 4594
SapI GCTCTTCn'nnn_
Cuts at: 2685 2685
Size: 6193
SbfI CC_TGCA'GG
Cuts at: 3545 3545
Size: 6193
ScaI AGT'ACT
Cuts at: 4441 6153 4441
Size: 1712 4481
SexAI A'CCwGG_T
Cuts at: 2193 2883 2193
Size: 690 5503
SfcI C'TryA_G
Cuts at: 826 1045 2431 3541 4676 5357 5548 826
Size: 219 1386 1110 1135 681 191 1471
Fragments arranged by size:
1471 1386 1135 1110 681 219 191
SfoI GGC'GCC
Cuts at: 2925 2925
Size: 6193
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6193
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6193
SmaI CCC'GGG
Cuts at: 1872 3520 1872
Size: 1648 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4297 5198 5460 820
Size: 197 926 2354 901 262 1553
Fragments arranged by size:
2354 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3984 493
Size: 3491 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6193
SphI G_CATG'C
Cuts at: 1132 3551 1132
Size: 2419 3774
SspI AAT'ATT
Cuts at: 5 52 4117 5
Size: 47 4065 2081
Fragments arranged by size:
4065 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6193
StyI C'CwwG_G
Cuts at: 513 1065 2004 2305 2532 3216 3246 3632 513
Size: 552 939 301 227 684 30 386 3074
Fragments arranged by size:
3074 939 684 552 386 301 227 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3468 4220 4237 4390 4575 5020 325
Size: 1255 1888 752 17 153 185 445 1498
Fragments arranged by size:
1888 1498 1255 752 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3412 4439 6151
Size: 274 80 33 51 1443 1435 1027 1712
Cuts at: 6151 96
Size: 138
Fragments arranged by size:
1712 1443 1435 1027 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2973 3005 6153 6185 2973
Size: 32 3148 32 2981
Fragments arranged by size:
3148 2981 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6193
XbaI T'CTAG_A
Cuts at: 3529 3529
Size: 6193
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6193
XmaI C'CCGG_G
Cuts at: 1870 3518 1870
Size: 1648 4545
XmnI GAAnn'nnTTC
Cuts at: 4322 4322
Size: 6193
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4001 276
Size: 53 83 186 936 2467 2468
Fragments arranged by size:
2468 2467 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI PvuII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI
PacI PmlI PspOMI PspXI RsrII SacI SacII SanDI
SfiI SrfI SwaI XcmI