Restriction map for
pFN21AE6406
(Circular) (Six-base) MAPSORT of: pFN21AE6406.seq Check: 2053 from: 1 to: 6079
pFN21AE6406 6079 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3889 278
Size: 53 83 186 936 2353 2468
Fragments arranged by size:
2468 2353 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3422 1937
Size: 1485 4594
Acc65I G'GTAC_C
Cuts at: 3400 3400
Size: 6079
AclI AA'CG_TT
Cuts at: 1563 2177 4206 4579 1563
Size: 614 2029 373 3063
Fragments arranged by size:
3063 2029 614 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2558 3219 4142 5526 5794 1632
Size: 926 661 923 1384 268 1917
Fragments arranged by size:
1917 1384 926 923 661 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5882
AflIII A'CryG_T
Cuts at: 1203 4978 5955 1203
Size: 3775 977 1327
Fragments arranged by size:
3775 1327 977
AgeI A'CCGG_T
Cuts at: 4910 4910
Size: 6079
AhdI GACnn_n'nnGTC
Cuts at: 4808 4808
Size: 6079
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6079
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6079
AlwNI CAG_nnn'CTG
Cuts at: 2441 3822 5394 2441
Size: 1381 1572 3126
Fragments arranged by size:
3126 1572 1381
ApaLI G'TGCA_C
Cuts at: 4136 5292 4136
Size: 1156 4923
ApoI r'AATT_y
Cuts at: 1493 2048 2116 2237 2285 2431 2471 2513 2927
Size: 555 68 121 48 146 40 42 414
Cuts at: 2927 3221 3388 3614 3766 5875 1493
Size: 294 167 226 152 2109 1697
Fragments arranged by size:
2109 1697 555 414 294 226 167 152
146 121 68 48 42 40
AseI AT'TA_AT
Cuts at: 160 1032 4633 160
Size: 872 3601 1606
Fragments arranged by size:
3601 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6079
AvaI C'yCGr_G
Cuts at: 1870 1943 3404 1870
Size: 73 1461 4545
Fragments arranged by size:
4545 1461 73
BamHI G'GATC_C
Cuts at: 3037 3139 3409 3776 3037
Size: 102 270 367 5340
Fragments arranged by size:
5340 367 270 102
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3400 4855 618
Size: 325 217 627 1613 1455 1842
Fragments arranged by size:
1842 1613 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3398 1391
Size: 2007 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3318 928
Size: 971 1419 3689
Fragments arranged by size:
3689 1419 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4270 4304 1903
Size: 34 2333 34 3678
Fragments arranged by size:
3678 2333 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3971 5181 3971
Size: 1210 4869
BclI T'GATC_A
Cuts at: 1548 2566 1548
Size: 1018 5061
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4690 136
Size: 107 122 71 631 605 337 2681 1525
Fragments arranged by size:
2681 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6074 6074
Size: 6079
BlpI GC'TnA_GC
Cuts at: 2305 3496 2305
Size: 1191 4888
Bme1580I G_kGCm'C
Cuts at: 1790 4140 5296 1790
Size: 2350 1156 2573
Fragments arranged by size:
2573 2350 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6079
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3810 4768 442
Size: 416 53 83 399 24 2393 958 1753
Fragments arranged by size:
2393 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6079
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4739 1239
Size: 3500 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2352 3110 4204 5069 5367 2352
Size: 758 1094 865 298 3064
Fragments arranged by size:
3064 1094 865 758 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4742 882
Size: 617 3243 2219
Fragments arranged by size:
3243 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2215 3870 493
Size: 711 1011 1655 2702
Fragments arranged by size:
2702 1655 1011 711
BsaBI GATnn'nnATC
Cuts at: 6073 6073
Size: 6079
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3886 4268 4903 275
Size: 53 83 186 936 2353 382 635 1451
Fragments arranged by size:
2353 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4512 4910 5184 5331 5742 4512
Size: 398 274 147 411 4849
Fragments arranged by size:
4849 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5854
Fragments arranged by size:
5854 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6079
BseYI C'CCAG_C
Cuts at: 3143 5282 5778 3143
Size: 2139 496 3444
Fragments arranged by size:
3444 2139 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2320 1163
Size: 573 584 4922
Fragments arranged by size:
4922 584 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3529 4290 5318 1540
Size: 275 186 1528 761 1028 2301
Fragments arranged by size:
2301 1528 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4140 4225 5296 4140
Size: 85 1071 4923
Fragments arranged by size:
4923 1071 85
BsmI GAATG_Cn'
Cuts at: 3590 3683 3590
Size: 93 5986
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3398 4140 4225 5296 1391
Size: 399 1608 742 85 1071 2174
Fragments arranged by size:
2174 1608 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5742 5742
Size: 6079
BspHI T'CATG_A
Cuts at: 2569 3968 5698 2569
Size: 1399 1730 2950
Fragments arranged by size:
2950 1730 1399
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2191 3434 844
Size: 1347 1243 3489
Fragments arranged by size:
3489 1347 1243
BsrBI CCG'CTC
Cuts at: 1394 1823 3966 1394
Size: 429 2143 3507
Fragments arranged by size:
3507 2143 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4574 4748 65
Size: 1183 3326 174 1396
Fragments arranged by size:
3326 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4723 4910 1700
Size: 110 2913 187 2869
Fragments arranged by size:
2913 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2198 2795 3149 96
Size: 2102 597 354 3026
Fragments arranged by size:
3026 2102 597 354
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6079
BssSI C'ACGA_G
Cuts at: 4139 5151 4139
Size: 1012 5067
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6079
BstBI TT'CG_AA
Cuts at: 3130 4883 3130
Size: 1753 4326
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6079
BstYI r'GATC_y
Cuts at: 1972 3037 3139 3409 3776 4161 4178 5630 5716
Size: 1065 102 270 367 385 17 1452 86
Cuts at: 5716 5728 6074 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1065 385 367 346 270 102
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4588
Fragments arranged by size:
4588 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5340
Fragments arranged by size:
5340 683 56
BtsI GCAGTG_nn'
Cuts at: 3100 3595 4389 4409 3100
Size: 495 794 20 4770
Fragments arranged by size:
4770 794 495 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6079
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6044
DraI TTT'AAA
Cuts at: 2155 3383 2155
Size: 1228 4851
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5086 809
Size: 507 3770 1802
Fragments arranged by size:
3770 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2350 3526 4415
Size: 54 1425 50 275 18 520 1176 889
Cuts at: 4415 6044 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1176 889 520 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3526 1537
Size: 275 1714 4090
Fragments arranged by size:
4090 1714 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3357 4009 1353
Size: 2004 652 3423
Fragments arranged by size:
3423 2004 652
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4666 5040 5186 387
Size: 1064 138 3077 374 146 1280
Fragments arranged by size:
3077 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2558 3219 4142 4739 5526 5794 1239
Size: 393 926 661 923 597 787 268 1524
Fragments arranged by size:
1524 926 923 787 661 597 393 268
EcoRI G'AATT_C
Cuts at: 2285 2513 3388 3766 2285
Size: 228 875 378 4598
Fragments arranged by size:
4598 875 378 228
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2513 3332 2513
Size: 819 5260
FspI TGC'GCA
Cuts at: 4585 4585
Size: 6079
HaeII r_GCGC'y
Cuts at: 1678 5226 1678
Size: 3548 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2429 2461 3275 3307 4720
Size: 32 135 32 857 32 814 32 1413
Cuts at: 4720 4752 4794 4826 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1413 857 814 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2032 3423 3669 669
Size: 988 281 94 1391 246 3079
Fragments arranged by size:
3079 1391 988 281 246 94
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6079
HpaI GTT'AAC
Cuts at: 3669 3669
Size: 6079
KpnI G_GTAC'C
Cuts at: 3404 3404
Size: 6079
MfeI C'AATT_G
Cuts at: 2547 3678 2547
Size: 1131 4948
MluI A'CGCG_T
Cuts at: 5955 5955
Size: 6079
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5193 5377 1305
Size: 3888 184 2007
Fragments arranged by size:
3888 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2352 10
Size: 54 1425 343 520 3737
Fragments arranged by size:
3737 1425 520 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3212 4037 4396 4555 518
Size: 648 2046 825 359 159 2042
Fragments arranged by size:
2046 2042 825 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2708 3147 3495 4172 5320 5565 1606
Size: 1102 439 348 677 1148 245 2120
Fragments arranged by size:
2120 1148 1102 677 439 348 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4588
Fragments arranged by size:
4588 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6079
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6079
NotI GC'GGCC_GC
Cuts at: 3526 3526
Size: 6079
NspI r_CATG'y
Cuts at: 1132 1229 3437 4982 1132
Size: 97 2208 1545 2229
Fragments arranged by size:
2229 2208 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6079
PciI A'CATG_T
Cuts at: 4978 4978
Size: 6079
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6079
PmeI GTTT'AAAC
Cuts at: 3383 3383
Size: 6079
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4148 4180 5661 5693 4148
Size: 32 1481 32 4534
Fragments arranged by size:
4534 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6079
PsiI TTA'TAA
Cuts at: 3649 3649
Size: 6079
PstI C_TGCA'G
Cuts at: 830 2188 3431 830
Size: 1358 1243 3478
Fragments arranged by size:
3478 1358 1243
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6079
PvuII CAG'CTG
Cuts at: 2708 3147 2708
Size: 439 5640
SalI G'TCGA_C
Cuts at: 1936 3421 1936
Size: 1485 4594
SbfI CC_TGCA'GG
Cuts at: 3431 3431
Size: 6079
ScaI AGT'ACT
Cuts at: 4327 6039 4327
Size: 1712 4367
SfcI C'TryA_G
Cuts at: 826 1045 2013 2184 2275 3427 4562 5243 5434
Size: 219 968 171 91 1152 1135 681 191
Cuts at: 5434 826
Size: 1471
Fragments arranged by size:
1471 1152 1135 968 681 219 191 171
91
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6079
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6079
SmaI CCC'GGG
Cuts at: 1872 3406 1872
Size: 1534 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2367 3089 4183 5084 5346 820
Size: 197 926 424 722 1094 901 262 1553
Fragments arranged by size:
1553 1094 926 901 722 424 262 197
SnaBI TAC'GTA
Cuts at: 493 3870 493
Size: 3377 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6079
SphI G_CATG'C
Cuts at: 1132 3437 1132
Size: 2305 3774
SspI AAT'ATT
Cuts at: 5 52 2425 2558 2957 4003 5
Size: 47 2373 133 399 1046 2081
Fragments arranged by size:
2373 2081 1046 399 133 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6079
StyI C'CwwG_G
Cuts at: 513 1065 2004 2628 3518 513
Size: 552 939 624 890 3074
Fragments arranged by size:
3074 939 890 624 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2074 2316 4106 4123 4276 4461 4906
Size: 1255 494 242 1790 17 153 185 445
Cuts at: 4906 325
Size: 1498
Fragments arranged by size:
1790 1498 1255 494 445 242 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2198 2795 3149
Size: 274 80 33 51 1443 221 597 354
Cuts at: 3149 4325 6037 96
Size: 1176 1712 138
Fragments arranged by size:
1712 1443 1176 597 354 274 221 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2694 2726 6039 6071 2694
Size: 32 3313 32 2702
Fragments arranged by size:
3313 2702 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6079
XbaI T'CTAG_A
Cuts at: 2258 3415 2258
Size: 1157 4922
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6079
XmaI C'CCGG_G
Cuts at: 1870 3404 1870
Size: 1534 4545
XmnI GAAnn'nnTTC
Cuts at: 2238 4208 2238
Size: 1970 4109
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3887 276
Size: 53 83 186 936 2353 2468
Fragments arranged by size:
2468 2353 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI