Restriction map for
pFN21AE6405
(Circular) (Six-base) MAPSORT of: pFN21AE6405.seq Check: 5411 from: 1 to: 6523
pFN21AE6405 6523 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2321 3412 3538 2321
Size: 1091 126 5306
Fragments arranged by size:
5306 1091 126
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4333 278
Size: 53 83 186 936 2797 2468
Fragments arranged by size:
2797 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2520 3447 3866 1937
Size: 583 927 419 4594
Fragments arranged by size:
4594 927 583 419
Acc65I G'GTAC_C
Cuts at: 3844 3844
Size: 6523
AclI AA'CG_TT
Cuts at: 1563 4650 5023 1563
Size: 3087 373 3063
Fragments arranged by size:
3087 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2108 2312 2759 3092 3458 4586 5970 6238
Size: 476 204 447 333 366 1128 1384 268
Cuts at: 6238 1632
Size: 1917
Fragments arranged by size:
1917 1384 1128 476 447 366 333 268
204
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6326
AflIII A'CryG_T
Cuts at: 1203 3063 5422 6399 1203
Size: 1860 2359 977 1327
Fragments arranged by size:
2359 1860 1327 977
AgeI A'CCGG_T
Cuts at: 5354 5354
Size: 6523
AhdI GACnn_n'nnGTC
Cuts at: 5252 5252
Size: 6523
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6523
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6523
AlwNI CAG_nnn'CTG
Cuts at: 2086 3253 3346 3464 3709 4266 5838 2086
Size: 1167 93 118 245 557 1572 2771
Fragments arranged by size:
2771 1572 1167 557 245 118 93
ApaI G_GGCC'C
Cuts at: 2022 2624 3363 2022
Size: 602 739 5182
Fragments arranged by size:
5182 739 602
ApaLI G'TGCA_C
Cuts at: 4580 5736 4580
Size: 1156 5367
ApoI r'AATT_y
Cuts at: 1493 3832 4058 4210 6319 1493
Size: 2339 226 152 2109 1697
Fragments arranged by size:
2339 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5077 160
Size: 872 4045 1606
Fragments arranged by size:
4045 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6523
AvaI C'yCGr_G
Cuts at: 1870 1943 2558 3580 3848 1870
Size: 73 615 1022 268 4545
Fragments arranged by size:
4545 1022 615 268 73
BamHI G'GATC_C
Cuts at: 3853 4220 3853
Size: 367 6156
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2036 3844 5299 618
Size: 325 217 627 249 1808 1455 1842
Fragments arranged by size:
1842 1808 1455 627 325 249 217
BanII G_rGCy'C
Cuts at: 1391 2022 2624 2808 3335 3363 3690 3842 1391
Size: 631 602 184 527 28 327 152 4072
Fragments arranged by size:
4072 631 602 527 327 184 152 28
BbeI G_GCGC'C
Cuts at: 2040 2040
Size: 6523
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5552
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4714 4748 1903
Size: 34 2777 34 3678
Fragments arranged by size:
3678 2777 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4415 5625 4415
Size: 1210 5313
BclI T'GATC_A
Cuts at: 1548 2302 1548
Size: 754 5769
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5134 136
Size: 107 122 71 631 605 3462 1525
Fragments arranged by size:
3462 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2053 3234 6518 2053
Size: 1181 3284 2058
Fragments arranged by size:
3284 2058 1181
BlpI GC'TnA_GC
Cuts at: 2474 2921 3940 2474
Size: 447 1019 5057
Fragments arranged by size:
5057 1019 447
Bme1580I G_kGCm'C
Cuts at: 1790 2022 2624 3278 3363 3664 4584 5740 1790
Size: 232 602 654 85 301 920 1156 2573
Fragments arranged by size:
2573 1156 920 654 602 301 232 85
BmgBI CAC'GTC
Cuts at: 1318 2452 1318
Size: 1134 5389
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2190 4254 5212
Size: 416 53 83 399 24 773 2064 958
Cuts at: 5212 442
Size: 1753
Fragments arranged by size:
2064 1753 958 773 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6523
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3196 3196
Size: 6523
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2051 2180 2356 2678 5183 1239
Size: 812 129 176 322 2505 2579
Fragments arranged by size:
2579 2505 812 322 176 129
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3191 3505 3770 4648 5513 5811 3191
Size: 314 265 878 865 298 3903
Fragments arranged by size:
3903 878 865 314 298 265
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2895 3184 5186 882
Size: 617 1396 289 2002 2219
Fragments arranged by size:
2219 2002 1396 617 289
BsaAI yAC'GTr
Cuts at: 493 1204 4314 493
Size: 711 3110 2702
Fragments arranged by size:
3110 2702 711
BsaBI GATnn'nnATC
Cuts at: 3809 6517 3809
Size: 2708 3815
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2037 4330 4712 5347
Size: 53 83 186 936 504 2293 382 635
Cuts at: 5347 275
Size: 1451
Fragments arranged by size:
2293 1451 936 635 504 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4956 5354 5628 5775 6186 4956
Size: 398 274 147 411 5293
Fragments arranged by size:
5293 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2780 2810 1178
Size: 30 165 30 1377 30 4891
Fragments arranged by size:
4891 1377 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3187 3801 1186
Size: 2001 614 3908
Fragments arranged by size:
3908 2001 614
BseYI C'CCAG_C
Cuts at: 2726 2979 3709 5726 6222 2726
Size: 253 730 2017 496 3027
Fragments arranged by size:
3027 2017 730 496 253
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3359 1163
Size: 573 1623 4327
Fragments arranged by size:
4327 1623 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2013 3973 4734 5762 1540
Size: 275 186 12 1960 761 1028 2301
Fragments arranged by size:
2301 1960 1028 761 275 186 12
BsiHKAI G_wGCw'C
Cuts at: 2358 2742 2808 3690 4584 4669 5740 2358
Size: 384 66 882 894 85 1071 3141
Fragments arranged by size:
3141 1071 894 882 384 85 66
BsmI GAATG_Cn'
Cuts at: 2704 2947 4034 4127 2704
Size: 243 1087 93 5100
Fragments arranged by size:
5100 1087 243 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3044 3044
Size: 6523
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2358 2624 2742 2808 3278 3335
Size: 399 232 336 266 118 66 470 57
Cuts at: 3335 3363 3664 3690 3842 4584 4669 5740 1391
Size: 28 301 26 152 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 470 399 336 301 266
232 152 118 85 66 57 28 26
BspEI T'CCGG_A
Cuts at: 6186 6186
Size: 6523
BspHI T'CATG_A
Cuts at: 4412 6142 4412
Size: 1730 4793
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2321 2328 3412 3538 3630 3878 844
Size: 1477 7 1084 126 92 248 3489
Fragments arranged by size:
3489 1477 1084 248 126 92 7
BsrBI CCG'CTC
Cuts at: 1394 1823 3175 4410 1394
Size: 429 1352 1235 3507
Fragments arranged by size:
3507 1352 1235 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5018 5192 65
Size: 1183 3770 174 1396
Fragments arranged by size:
3770 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2466 5167 5354 1700
Size: 110 656 2701 187 2869
Fragments arranged by size:
2869 2701 656 187 110
BsrGI T'GTAC_A
Cuts at: 96 2814 96
Size: 2718 3805
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6523
BssSI C'ACGA_G
Cuts at: 4583 5595 4583
Size: 1012 5511
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6523
BstBI TT'CG_AA
Cuts at: 5327 5327
Size: 6523
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6523
BstXI CCAn_nnnn'nTGG
Cuts at: 3520 3520
Size: 6523
BstYI r'GATC_y
Cuts at: 1972 2053 3090 3220 3234 3480 3853 4220 4605
Size: 81 1037 130 14 246 373 367 385
Cuts at: 4605 4622 6074 6160 6172 6518 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1037 385 373 367 346 246
130 86 81 17 14 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2598 3364 513
Size: 552 939 594 766 3672
Fragments arranged by size:
3672 939 766 594 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2854 487
Size: 683 56 1628 4156
Fragments arranged by size:
4156 1628 683 56
BtsI GCAGTG_nn'
Cuts at: 3645 4039 4833 4853 3645
Size: 394 794 20 5315
Fragments arranged by size:
5315 794 394 20
ClaI AT'CG_AT
Cuts at: 1554 2421 1554
Size: 867 5656
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6488
DraI TTT'AAA
Cuts at: 3827 3827
Size: 6523
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5530 809
Size: 507 4214 1802
Fragments arranged by size:
4214 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2484 3134 3700
Size: 54 1425 50 275 18 654 650 566
Cuts at: 3700 3970 4859 6488 8
Size: 270 889 1629 43
Fragments arranged by size:
1629 1425 889 654 650 566 275 270
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3970 1537
Size: 275 2158 4090
Fragments arranged by size:
4090 2158 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2288 3620 4453 1353
Size: 935 1332 833 3423
Fragments arranged by size:
3423 1332 935 833
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5110 5484 5630 387
Size: 1064 138 3521 374 146 1280
Fragments arranged by size:
3521 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2806 3688 2806
Size: 882 5641
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2051 2108 2180 2312 2356 2678 2759
Size: 393 419 57 72 132 44 322 81
Cuts at: 2759 3092 3458 4586 5183 5970 6238 1239
Size: 333 366 1128 597 787 268 1524
Fragments arranged by size:
1524 1128 787 597 419 393 366 333
322 268 132 81 72 57 44
EcoNI CCTnn'n_nnAGG
Cuts at: 3215 3440 3668 3215
Size: 225 228 6070
Fragments arranged by size:
6070 228 225
EcoO109I rG'GnC_Cy
Cuts at: 2018 2212 2344 2621 3360 3408 3535 2018
Size: 194 132 277 739 48 127 5006
Fragments arranged by size:
5006 739 277 194 132 127 48
EcoRI G'AATT_C
Cuts at: 3832 4210 3832
Size: 378 6145
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2962 3130 3307 3412 2962
Size: 168 177 105 6073
Fragments arranged by size:
6073 177 168 105
FspI TGC'GCA
Cuts at: 5029 5029
Size: 6523
HaeII r_GCGC'y
Cuts at: 1678 2040 5670 1678
Size: 362 3630 2531
Fragments arranged by size:
3630 2531 362
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2182 2214 2287 2319 2780
Size: 32 135 32 610 32 73 32 461
Cuts at: 2780 2812 3560 3592 5164 5196 5238 5270 1373
Size: 32 748 32 1572 32 42 32 2626
Fragments arranged by size:
2626 1572 748 610 461 135 73 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3867 4113 669
Size: 988 281 1929 246 3079
Fragments arranged by size:
3079 1929 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6523
HpaI GTT'AAC
Cuts at: 4113 4113
Size: 6523
KasI G'GCGC_C
Cuts at: 2036 2036
Size: 6523
KpnI G_GTAC'C
Cuts at: 3848 3848
Size: 6523
MfeI C'AATT_G
Cuts at: 4122 4122
Size: 6523
MluI A'CGCG_T
Cuts at: 6399 6399
Size: 6523
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3537 5637 5821 1305
Size: 2232 2100 184 2007
Fragments arranged by size:
2232 2100 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2486 3136 10
Size: 54 1425 343 654 650 3397
Fragments arranged by size:
3397 1425 654 650 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2630 3728 4481 4840 4999 518
Size: 648 1464 1098 753 359 159 2042
Fragments arranged by size:
2042 1464 1098 753 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2071 2889 2970 3140 3709 3939 4616 5764
Size: 465 818 81 170 569 230 677 1148
Cuts at: 5764 6009 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 818 677 569 465 245 230
170 81
NaeI GCC'GGC
Cuts at: 2468 2468
Size: 6523
NarI GG'CG_CC
Cuts at: 2037 2037
Size: 6523
NcoI C'CATG_G
Cuts at: 513 1065 2004 3364 513
Size: 552 939 1360 3672
Fragments arranged by size:
3672 1360 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6523
NgoMIV G'CCGG_C
Cuts at: 2466 2466
Size: 6523
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6523
NotI GC'GGCC_GC
Cuts at: 3970 3970
Size: 6523
NspI r_CATG'y
Cuts at: 1132 1229 2686 2735 3067 3881 5426 1132
Size: 97 1457 49 332 814 1545 2229
Fragments arranged by size:
2229 1545 1457 814 332 97 49
PasI CC'CwG_GG
Cuts at: 1348 3128 1348
Size: 1780 4743
PciI A'CATG_T
Cuts at: 3063 5422 3063
Size: 2359 4164
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2452 3550 3709 1108
Size: 1344 1098 159 3922
Fragments arranged by size:
3922 1344 1098 159
PmeI GTTT'AAAC
Cuts at: 3827 3827
Size: 6523
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4592 4624 6105 6137 4592
Size: 32 1481 32 4978
Fragments arranged by size:
4978 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2212 2344 3408 2212
Size: 132 1064 5327
Fragments arranged by size:
5327 1064 132
PshAI GACnn'nnGTC
Cuts at: 1613 2907 1613
Size: 1294 5229
PsiI TTA'TAA
Cuts at: 4093 4093
Size: 6523
PspOMI G'GGCC_C
Cuts at: 2018 2620 3359 2018
Size: 602 739 5182
Fragments arranged by size:
5182 739 602
PstI C_TGCA'G
Cuts at: 830 2082 2766 3627 3875 830
Size: 1252 684 861 248 3478
Fragments arranged by size:
3478 1252 861 684 248
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6523
PvuII CAG'CTG
Cuts at: 2970 3709 2970
Size: 739 5784
SacI G_AGCT'C
Cuts at: 2808 3690 2808
Size: 882 5641
SalI G'TCGA_C
Cuts at: 1936 3865 1936
Size: 1929 4594
SanDI GG'GwC_CC
Cuts at: 3408 3408
Size: 6523
SapI GCTCTTCn'nnn_
Cuts at: 2288 2288
Size: 6523
SbfI CC_TGCA'GG
Cuts at: 3875 3875
Size: 6523
ScaI AGT'ACT
Cuts at: 2204 4771 6483 2204
Size: 2567 1712 2244
Fragments arranged by size:
2567 2244 1712
SexAI A'CCwGG_T
Cuts at: 2178 2178
Size: 6523
SfcI C'TryA_G
Cuts at: 826 1045 2078 2719 2762 3623 3871 5006 5687
Size: 219 1033 641 43 861 248 1135 681
Cuts at: 5687 5878 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1033 861 681 641 248 219
191 43
SfoI GGC'GCC
Cuts at: 2038 2038
Size: 6523
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6523
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6523
SmaI CCC'GGG
Cuts at: 1872 3582 3850 1872
Size: 1710 268 4545
Fragments arranged by size:
4545 1710 268
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3170 3484 3749 4627 5528 5790
Size: 197 926 1227 314 265 878 901 262
Cuts at: 5790 820
Size: 1553
Fragments arranged by size:
1553 1227 926 901 878 314 265 262
197
SnaBI TAC'GTA
Cuts at: 493 4314 493
Size: 3821 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6523
SphI G_CATG'C
Cuts at: 1132 3881 1132
Size: 2749 3774
SspI AAT'ATT
Cuts at: 5 52 4447 5
Size: 47 4395 2081
Fragments arranged by size:
4395 2081 47
StuI AGG'CCT
Cuts at: 1517 3507 1517
Size: 1990 4533
StyI C'CwwG_G
Cuts at: 513 1065 2004 2799 3364 3765 3962 513
Size: 552 939 795 565 401 197 3074
Fragments arranged by size:
3074 939 795 565 552 401 197
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2993 3296 4550 4567 4720 4905 5350
Size: 1255 1413 303 1254 17 153 185 445
Cuts at: 5350 325
Size: 1498
Fragments arranged by size:
1498 1413 1255 1254 445 303 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2202 2814 3323
Size: 274 80 33 51 1443 225 612 509
Cuts at: 3323 4769 6481 96
Size: 1446 1712 138
Fragments arranged by size:
1712 1446 1443 612 509 274 225 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6483 6515 6483
Size: 32 6491
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6523
XbaI T'CTAG_A
Cuts at: 3859 3859
Size: 6523
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6523
XmaI C'CCGG_G
Cuts at: 1870 3580 3848 1870
Size: 1710 268 4545
Fragments arranged by size:
4545 1710 268
XmnI GAAnn'nnTTC
Cuts at: 4652 4652
Size: 6523
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4331 276
Size: 53 83 186 936 2797 2468
Fragments arranged by size:
2797 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NruI
NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII
SfiI SrfI SwaI XcmI