Restriction map for
pFN21AE6395
(Circular) (Six-base) MAPSORT of: pFN21AE6395.seq Check: 6790 from: 1 to: 5944
pFN21AE6395 5944 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3754 278
Size: 53 83 186 936 2218 2468
Fragments arranged by size:
2468 2218 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3111 3287 1937
Size: 1174 176 4594
Fragments arranged by size:
4594 1174 176
Acc65I G'GTAC_C
Cuts at: 2104 2657 3265 2104
Size: 553 608 4783
Fragments arranged by size:
4783 608 553
AclI AA'CG_TT
Cuts at: 1563 3162 4071 4444 1563
Size: 1599 909 373 3063
Fragments arranged by size:
3063 1599 909 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2870 4007 5391 5659 1632
Size: 1238 1137 1384 268 1917
Fragments arranged by size:
1917 1384 1238 1137 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5747
AflIII A'CryG_T
Cuts at: 1203 4843 5820 1203
Size: 3640 977 1327
Fragments arranged by size:
3640 1327 977
AgeI A'CCGG_T
Cuts at: 3041 4775 3041
Size: 1734 4210
AhdI GACnn_n'nnGTC
Cuts at: 4673 4673
Size: 5944
AleI CACnn'nnGTG
Cuts at: 1166 2444 1166
Size: 1278 4666
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5944
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2399 2431 2399
Size: 32 5912
AlwNI CAG_nnn'CTG
Cuts at: 3687 5259 3687
Size: 1572 4372
ApaLI G'TGCA_C
Cuts at: 4001 5157 4001
Size: 1156 4788
ApoI r'AATT_y
Cuts at: 1493 3253 3479 3631 5740 1493
Size: 1760 226 152 2109 1697
Fragments arranged by size:
2109 1760 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4498 160
Size: 872 3466 1606
Fragments arranged by size:
3466 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5944
AvaI C'yCGr_G
Cuts at: 1870 1943 2760 3269 1870
Size: 73 817 509 4545
Fragments arranged by size:
4545 817 509 73
BamHI G'GATC_C
Cuts at: 3274 3641 3274
Size: 367 5577
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2104 2276 2381 2657 3120
Size: 325 217 627 317 172 105 276 463
Cuts at: 3120 3265 4720 618
Size: 145 1455 1842
Fragments arranged by size:
1842 1455 627 463 325 317 276 217
172 145 105
BanII G_rGCy'C
Cuts at: 1391 3038 3263 1391
Size: 1647 225 4072
Fragments arranged by size:
4072 1647 225
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4973
BbvCI CC'TCA_GC
Cuts at: 2487 2487
Size: 5944
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4135 4169 1903
Size: 34 2198 34 3678
Fragments arranged by size:
3678 2198 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3836 5046 3836
Size: 1210 4734
BclI T'GATC_A
Cuts at: 1548 2956 1548
Size: 1408 4536
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4555 136
Size: 107 122 71 631 605 2883 1525
Fragments arranged by size:
2883 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5939 5939
Size: 5944
BlpI GC'TnA_GC
Cuts at: 3361 3361
Size: 5944
Bme1580I G_kGCm'C
Cuts at: 1790 2797 3123 4005 5161 1790
Size: 1007 326 882 1156 2573
Fragments arranged by size:
2573 1156 1007 882 326
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5944
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3675 4633 442
Size: 416 53 83 399 24 2258 958 1753
Fragments arranged by size:
2258 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5944
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3052 3052
Size: 5944
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2864 4604 1239
Size: 1625 1740 2579
Fragments arranged by size:
2579 1740 1625
Bpu10I CC'TnA_GC
Cuts at: 2487 2555 2487
Size: 68 5876
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2116 2339 4069 4934 5232 2116
Size: 223 1730 865 298 2828
Fragments arranged by size:
2828 1730 865 298 223
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4607 882
Size: 617 3108 2219
Fragments arranged by size:
3108 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3735 493
Size: 711 2531 2702
Fragments arranged by size:
2702 2531 711
BsaBI GATnn'nnATC
Cuts at: 5938 5938
Size: 5944
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3751 4133 4768 275
Size: 53 83 186 936 2218 382 635 1451
Fragments arranged by size:
2218 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3041 4377 4775 5049 5196 5607 3041
Size: 1336 398 274 147 411 3378
Fragments arranged by size:
3378 1336 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2340 2370 1178
Size: 30 165 30 937 30 4752
Fragments arranged by size:
4752 937 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2632 2814 1186
Size: 1446 182 4316
Fragments arranged by size:
4316 1446 182
BseYI C'CCAG_C
Cuts at: 2222 2790 5147 5643 2222
Size: 568 2357 496 2523
Fragments arranged by size:
2523 2357 568 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5371
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3394 4155 5183 1540
Size: 275 186 1393 761 1028 2301
Fragments arranged by size:
2301 1393 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2860 4005 4090 5161 2860
Size: 1145 85 1071 3643
Fragments arranged by size:
3643 1145 1071 85
BsmI GAATG_Cn'
Cuts at: 2204 2472 3455 3548 2204
Size: 268 983 93 4600
Fragments arranged by size:
4600 983 268 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2797 2860 3038 3123 3263 4005 4090
Size: 399 1007 63 178 85 140 742 85
Cuts at: 4090 5161 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1007 742 399 178 140 85
85 63
BspEI T'CCGG_A
Cuts at: 5607 5607
Size: 5944
BspHI T'CATG_A
Cuts at: 2518 3833 5563 2518
Size: 1315 1730 2899
Fragments arranged by size:
2899 1730 1315
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2382 3299 844
Size: 1538 917 3489
Fragments arranged by size:
3489 1538 917
BsrBI CCG'CTC
Cuts at: 1394 1823 3831 1394
Size: 429 2008 3507
Fragments arranged by size:
3507 2008 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3102 4439 4613 65
Size: 1183 1854 1337 174 1396
Fragments arranged by size:
1854 1396 1337 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3041 4588 4775 1700
Size: 110 1231 1547 187 2869
Fragments arranged by size:
2869 1547 1231 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5944
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5944
BssSI C'ACGA_G
Cuts at: 2796 4004 5016 2796
Size: 1208 1012 3724
Fragments arranged by size:
3724 1208 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2199 794
Size: 1405 4539
BstBI TT'CG_AA
Cuts at: 4748 4748
Size: 5944
BstEII G'GTnAC_C
Cuts at: 1184 2642 1184
Size: 1458 4486
BstYI r'GATC_y
Cuts at: 1972 2063 3070 3274 3641 4026 4043 5495 5581
Size: 91 1007 204 367 385 17 1452 86
Cuts at: 5581 5593 5939 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1007 385 367 346 204 91
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2457 3139 513
Size: 552 939 453 682 3318
Fragments arranged by size:
3318 939 682 552 453
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3036 487
Size: 683 56 1810 3395
Fragments arranged by size:
3395 1810 683 56
BtsI GCAGTG_nn'
Cuts at: 2986 3460 4254 4274 2986
Size: 474 794 20 4656
Fragments arranged by size:
4656 794 474 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5944
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5909
DraI TTT'AAA
Cuts at: 3248 3248
Size: 5944
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4951 809
Size: 507 3635 1802
Fragments arranged by size:
3635 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2713 3391 4280
Size: 54 1425 50 275 18 883 678 889
Cuts at: 4280 5909 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 883 678 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3391 1537
Size: 275 1579 4090
Fragments arranged by size:
4090 1579 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2844 3874 1353
Size: 1491 1030 3423
Fragments arranged by size:
3423 1491 1030
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4531 4905 5051 387
Size: 1064 138 2942 374 146 1280
Fragments arranged by size:
2942 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2864 2870 4007 4604 5391 5659 1239
Size: 393 1232 6 1137 597 787 268 1524
Fragments arranged by size:
1524 1232 1137 787 597 393 268 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2290 2290
Size: 5944
EcoRI G'AATT_C
Cuts at: 3253 3631 3253
Size: 378 5566
FspI TGC'GCA
Cuts at: 4450 4450
Size: 5944
HaeII r_GCGC'y
Cuts at: 1678 5091 1678
Size: 3413 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2119 2151 3014 3046 4585
Size: 32 135 32 547 32 863 32 1539
Cuts at: 4585 4617 4659 4691 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1539 863 547 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3288 3534 669
Size: 988 281 1350 246 3079
Fragments arranged by size:
3079 1350 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2450 748
Size: 1702 4242
HpaI GTT'AAC
Cuts at: 3534 3534
Size: 5944
KpnI G_GTAC'C
Cuts at: 2108 2661 3269 2108
Size: 553 608 4783
Fragments arranged by size:
4783 608 553
MfeI C'AATT_G
Cuts at: 3229 3543 3229
Size: 314 5630
MluI A'CGCG_T
Cuts at: 5820 5820
Size: 5944
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3012 5058 5242 1305
Size: 1707 2046 184 2007
Fragments arranged by size:
2046 2007 1707 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4122
Fragments arranged by size:
4122 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2444 3902 4261 4420 518
Size: 648 1278 1458 359 159 2042
Fragments arranged by size:
2042 1458 1278 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2444 3360 4037 5185 5430 1606
Size: 838 916 677 1148 245 2120
Fragments arranged by size:
2120 1148 916 838 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4453
Fragments arranged by size:
4453 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5944
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5944
NotI GC'GGCC_GC
Cuts at: 3391 3391
Size: 5944
NspI r_CATG'y
Cuts at: 1132 1229 2495 3302 4847 1132
Size: 97 1266 807 1545 2229
Fragments arranged by size:
2229 1545 1266 807 97
PasI CC'CwG_GG
Cuts at: 1348 2042 1348
Size: 694 5250
PciI A'CATG_T
Cuts at: 4843 4843
Size: 5944
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3130 1108
Size: 2022 3922
PmeI GTTT'AAAC
Cuts at: 3248 3248
Size: 5944
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4013 4045 5526 5558 4013
Size: 32 1481 32 4399
Fragments arranged by size:
4399 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2762 1613
Size: 1149 4795
PsiI TTA'TAA
Cuts at: 3514 3514
Size: 5944
PstI C_TGCA'G
Cuts at: 830 2676 3296 830
Size: 1846 620 3478
Fragments arranged by size:
3478 1846 620
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5944
PvuII CAG'CTG
Cuts at: 2444 2444
Size: 5944
SalI G'TCGA_C
Cuts at: 1936 3286 1936
Size: 1350 4594
SapI GCTCTTCn'nnn_
Cuts at: 2844 2844
Size: 5944
SbfI CC_TGCA'GG
Cuts at: 3296 3296
Size: 5944
ScaI AGT'ACT
Cuts at: 4192 5904 4192
Size: 1712 4232
SfcI C'TryA_G
Cuts at: 826 1045 2464 2672 3292 4427 5108 5299 826
Size: 219 1419 208 620 1135 681 191 1471
Fragments arranged by size:
1471 1419 1135 681 620 219 208 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5944
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5944
SmaI CCC'GGG
Cuts at: 1872 2762 3271 1872
Size: 890 509 4545
Fragments arranged by size:
4545 890 509
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2095 2318 4048 4949 5211 820
Size: 197 926 152 223 1730 901 262 1553
Fragments arranged by size:
1730 1553 926 901 262 223 197 152
SnaBI TAC'GTA
Cuts at: 493 3735 493
Size: 3242 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5944
SphI G_CATG'C
Cuts at: 1132 3302 1132
Size: 2170 3774
SspI AAT'ATT
Cuts at: 5 52 3868 5
Size: 47 3816 2081
Fragments arranged by size:
3816 2081 47
StuI AGG'CCT
Cuts at: 1517 2743 1517
Size: 1226 4718
StyI C'CwwG_G
Cuts at: 513 1065 2004 2820 3383 513
Size: 552 939 816 563 3074
Fragments arranged by size:
3074 939 816 563 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2034 3118 3971 3988 4141 4326 4771
Size: 1255 454 1084 853 17 153 185 445
Cuts at: 4771 325
Size: 1498
Fragments arranged by size:
1498 1255 1084 853 454 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4190 5902 96
Size: 274 80 33 51 1443 2213 1712 138
Fragments arranged by size:
2213 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2072 2104 2136 2572 2604 5904 5936 2072
Size: 32 32 436 32 3300 32 2080
Fragments arranged by size:
3300 2080 436 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2534 1316
Size: 1218 4726
XbaI T'CTAG_A
Cuts at: 3280 3280
Size: 5944
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5944
XmaI C'CCGG_G
Cuts at: 1870 2760 3269 1870
Size: 890 509 4545
Fragments arranged by size:
4545 890 509
XmnI GAAnn'nnTTC
Cuts at: 4073 4073
Size: 5944
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3752 276
Size: 53 83 186 936 2218 2468
Fragments arranged by size:
2468 2218 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI