Restriction map for
pFN21AE6392
(Circular) (Six-base) MAPSORT of: pFN21AE6392.seq Check: 785 from: 1 to: 5959
pFN21AE6392 5959 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3769 278
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3207 3302 1937
Size: 1270 95 4594
Fragments arranged by size:
4594 1270 95
Acc65I G'GTAC_C
Cuts at: 3280 3280
Size: 5959
AclI AA'CG_TT
Cuts at: 1563 4086 4459 1563
Size: 2523 373 3063
Fragments arranged by size:
3063 2523 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2069 2600 4022 5406 5674 1632
Size: 437 531 1422 1384 268 1917
Fragments arranged by size:
1917 1422 1384 531 437 268
AflII C'TTAA_G
Cuts at: 820 1017 2225 820
Size: 197 1208 4554
Fragments arranged by size:
4554 1208 197
AflIII A'CryG_T
Cuts at: 1203 4858 5835 1203
Size: 3655 977 1327
Fragments arranged by size:
3655 1327 977
AgeI A'CCGG_T
Cuts at: 4790 4790
Size: 5959
AhdI GACnn_n'nnGTC
Cuts at: 2109 3119 4688 2109
Size: 1010 1569 3380
Fragments arranged by size:
3380 1569 1010
AleI CACnn'nnGTG
Cuts at: 1166 3215 1166
Size: 2049 3910
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2701 1184
Size: 1517 4442
AlwNI CAG_nnn'CTG
Cuts at: 3702 5274 3702
Size: 1572 4387
ApaLI G'TGCA_C
Cuts at: 4016 5172 4016
Size: 1156 4803
ApoI r'AATT_y
Cuts at: 1493 2172 2251 2440 2911 3081 3268 3494 3646
Size: 679 79 189 471 170 187 226 152
Cuts at: 3646 5755 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 679 471 226 189 187 170
152 79
AseI AT'TA_AT
Cuts at: 160 1032 4513 160
Size: 872 3481 1606
Fragments arranged by size:
3481 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
AvaI C'yCGr_G
Cuts at: 1870 1943 3284 1870
Size: 73 1341 4545
Fragments arranged by size:
4545 1341 73
BamHI G'GATC_C
Cuts at: 3289 3656 3289
Size: 367 5592
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2030 3280 4735 618
Size: 325 217 627 243 1250 1455 1842
Fragments arranged by size:
1842 1455 1250 627 325 243 217
BanII G_rGCy'C
Cuts at: 1391 2495 3278 1391
Size: 1104 783 4072
Fragments arranged by size:
4072 1104 783
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4988
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4150 4184 1903
Size: 34 2213 34 3678
Fragments arranged by size:
3678 2213 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3851 5061 3851
Size: 1210 4749
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5959
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4570 136
Size: 107 122 71 631 605 2898 1525
Fragments arranged by size:
2898 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5954 5954
Size: 5959
BlpI GC'TnA_GC
Cuts at: 3376 3376
Size: 5959
Bme1580I G_kGCm'C
Cuts at: 1790 4020 5176 1790
Size: 2230 1156 2573
Fragments arranged by size:
2573 2230 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5959
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3690 4648 442
Size: 416 53 83 399 24 2273 958 1753
Fragments arranged by size:
2273 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5959
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2310 2592 3222 4619 1239
Size: 1071 282 630 1397 2579
Fragments arranged by size:
2579 1397 1071 630 282
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4084 4949 5247 4084
Size: 865 298 4796
Fragments arranged by size:
4796 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2117 2842 4622 882
Size: 617 618 725 1780 2219
Fragments arranged by size:
2219 1780 725 618 617
BsaAI yAC'GTr
Cuts at: 493 1204 3144 3750 493
Size: 711 1940 606 2702
Fragments arranged by size:
2702 1940 711 606
BsaBI GATnn'nnATC
Cuts at: 5953 5953
Size: 5959
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3766 4148 4783 275
Size: 53 83 186 936 2233 382 635 1451
Fragments arranged by size:
2233 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4392 4790 5064 5211 5622 4392
Size: 398 274 147 411 4729
Fragments arranged by size:
4729 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5734
Fragments arranged by size:
5734 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5959
BseYI C'CCAG_C
Cuts at: 3168 5162 5658 3168
Size: 1994 496 3469
Fragments arranged by size:
3469 1994 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2852 1163
Size: 573 1116 4270
Fragments arranged by size:
4270 1116 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2247 3409 4170 5198 1540
Size: 275 186 246 1162 761 1028 2301
Fragments arranged by size:
2301 1162 1028 761 275 246 186
BsiHKAI G_wGCw'C
Cuts at: 2495 2944 4020 4105 5176 2495
Size: 449 1076 85 1071 3278
Fragments arranged by size:
3278 1076 1071 449 85
BsmI GAATG_Cn'
Cuts at: 3470 3563 3470
Size: 93 5866
BsmBI CGTCTCn'nnnn_
Cuts at: 2234 2234
Size: 5959
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2495 2944 3278 4020 4105 5176 1391
Size: 399 705 449 334 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 705 449 399 334 85
BspEI T'CCGG_A
Cuts at: 5622 5622
Size: 5959
BspHI T'CATG_A
Cuts at: 3848 5578 3848
Size: 1730 4229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2824 3314 844
Size: 1980 490 3489
Fragments arranged by size:
3489 1980 490
BsrBI CCG'CTC
Cuts at: 1394 1823 2799 3846 1394
Size: 429 976 1047 3507
Fragments arranged by size:
3507 1047 976 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2493 2577 2820 3043 4454 4628 65
Size: 1183 1245 84 243 223 1411 174 1396
Fragments arranged by size:
1411 1396 1245 1183 243 223 174 84
BsrFI r'CCGG_y
Cuts at: 1700 1810 4603 4790 1700
Size: 110 2793 187 2869
Fragments arranged by size:
2869 2793 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5959
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5959
BssSI C'ACGA_G
Cuts at: 4019 5031 4019
Size: 1012 4947
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5959
BstBI TT'CG_AA
Cuts at: 4763 4763
Size: 5959
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5959
BstXI CCAn_nnnn'nTGG
Cuts at: 2639 2715 2639
Size: 76 5883
BstYI r'GATC_y
Cuts at: 1972 2179 3130 3289 3656 4041 4058 5510 5596
Size: 207 951 159 367 385 17 1452 86
Cuts at: 5596 5608 5954 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 951 385 367 346 207 159
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2054 513
Size: 552 989 4418
Fragments arranged by size:
4418 989 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5220
Fragments arranged by size:
5220 683 56
BtsI GCAGTG_nn'
Cuts at: 2106 2673 2683 3475 4269 4289 2106
Size: 567 10 792 794 20 3776
Fragments arranged by size:
3776 794 792 567 20 10
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5959
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5924
DraI TTT'AAA
Cuts at: 2170 3263 2170
Size: 1093 4866
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4966 809
Size: 507 3650 1802
Fragments arranged by size:
3650 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3406 4295 5924
Size: 54 1425 50 275 18 1576 889 1629
Cuts at: 5924 8
Size: 43
Fragments arranged by size:
1629 1576 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3406 1537
Size: 275 1594 4090
Fragments arranged by size:
4090 1594 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2845 3889 1353
Size: 1492 1044 3423
Fragments arranged by size:
3423 1492 1044
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4546 4920 5066 387
Size: 1064 138 2957 374 146 1280
Fragments arranged by size:
2957 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2493 2493
Size: 5959
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2069 2310 2592 2600 3222 4022 4619
Size: 393 437 241 282 8 622 800 597
Cuts at: 4619 5406 5674 1239
Size: 787 268 1524
Fragments arranged by size:
1524 800 787 622 597 437 393 282
268 241 8
EcoO109I rG'GnC_Cy
Cuts at: 3113 3113
Size: 5959
EcoRI G'AATT_C
Cuts at: 3268 3646 3268
Size: 378 5581
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2630 2630
Size: 5959
FspI TGC'GCA
Cuts at: 4465 4465
Size: 5959
HaeII r_GCGC'y
Cuts at: 1678 5106 1678
Size: 3428 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2095 2127 2766 2798 2979
Size: 32 135 32 523 32 639 32 181
Cuts at: 2979 3011 3105 3137 4600 4632 4674 4706 1373
Size: 32 94 32 1463 32 42 32 2626
Fragments arranged by size:
2626 1463 639 523 181 135 94 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2511 2557 3303 3549 669
Size: 988 281 573 46 746 246 3079
Fragments arranged by size:
3079 988 746 573 281 246 46
HindIII A'AGCT_T
Cuts at: 748 2607 748
Size: 1859 4100
HpaI GTT'AAC
Cuts at: 2511 3549 2511
Size: 1038 4921
KpnI G_GTAC'C
Cuts at: 3284 3284
Size: 5959
MfeI C'AATT_G
Cuts at: 3558 3558
Size: 5959
MluI A'CGCG_T
Cuts at: 5835 5835
Size: 5959
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2929 5073 5257 1305
Size: 1624 2144 184 2007
Fragments arranged by size:
2144 2007 1624 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4137
Fragments arranged by size:
4137 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2360 2637 2713 3215 3917 4276 4435
Size: 648 1194 277 76 502 702 359 159
Cuts at: 4435 518
Size: 2042
Fragments arranged by size:
2042 1194 702 648 502 359 277 159
76
MspA1I CmG'CkG
Cuts at: 1606 3375 4052 5200 5445 1606
Size: 1769 677 1148 245 2120
Fragments arranged by size:
2120 1769 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2054 513
Size: 552 989 4418
Fragments arranged by size:
4418 989 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5959
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5959
NotI GC'GGCC_GC
Cuts at: 3406 3406
Size: 5959
NspI r_CATG'y
Cuts at: 1132 1229 3317 4862 1132
Size: 97 2088 1545 2229
Fragments arranged by size:
2229 2088 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5959
PciI A'CATG_T
Cuts at: 4858 4858
Size: 5959
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5959
PmeI GTTT'AAAC
Cuts at: 3263 3263
Size: 5959
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4028 4060 5541 5573 4028
Size: 32 1481 32 4414
Fragments arranged by size:
4414 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3113 3113
Size: 5959
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5959
PsiI TTA'TAA
Cuts at: 3529 3529
Size: 5959
PstI C_TGCA'G
Cuts at: 830 2680 2806 3098 3311 830
Size: 1850 126 292 213 3478
Fragments arranged by size:
3478 1850 292 213 126
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5959
SacI G_AGCT'C
Cuts at: 2495 2495
Size: 5959
SalI G'TCGA_C
Cuts at: 1936 3301 1936
Size: 1365 4594
SbfI CC_TGCA'GG
Cuts at: 3098 3311 3098
Size: 213 5746
ScaI AGT'ACT
Cuts at: 2153 4207 5919 2153
Size: 2054 1712 2193
Fragments arranged by size:
2193 2054 1712
SfcI C'TryA_G
Cuts at: 826 1045 2676 2802 3094 3307 4442 5123 5314
Size: 219 1631 126 292 213 1135 681 191
Cuts at: 5314 826
Size: 1471
Fragments arranged by size:
1631 1471 1135 681 292 219 213 191
126
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5959
SmaI CCC'GGG
Cuts at: 1872 3286 1872
Size: 1414 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2225 4063 4964 5226 820
Size: 197 926 282 1838 901 262 1553
Fragments arranged by size:
1838 1553 926 901 282 262 197
SnaBI TAC'GTA
Cuts at: 493 3750 493
Size: 3257 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5959
SphI G_CATG'C
Cuts at: 1132 3317 1132
Size: 2185 3774
SspI AAT'ATT
Cuts at: 5 52 3883 5
Size: 47 3831 2081
Fragments arranged by size:
3831 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5959
StyI C'CwwG_G
Cuts at: 513 1065 2054 2586 2600 3398 513
Size: 552 989 532 14 798 3074
Fragments arranged by size:
3074 989 798 552 532 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2261 2599 3986 4003 4156 4341 4786
Size: 1255 681 338 1387 17 153 185 445
Cuts at: 4786 325
Size: 1498
Fragments arranged by size:
1498 1387 1255 681 445 338 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2151 4205 5917
Size: 274 80 33 51 1443 174 2054 1712
Cuts at: 5917 96
Size: 138
Fragments arranged by size:
2054 1712 1443 274 174 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2673 2705 5919 5951 2673
Size: 32 3214 32 2681
Fragments arranged by size:
3214 2681 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3008 1316
Size: 1692 4267
XbaI T'CTAG_A
Cuts at: 3295 3295
Size: 5959
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2113 2525 2113
Size: 412 5547
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5959
XmaI C'CCGG_G
Cuts at: 1870 3284 1870
Size: 1414 4545
XmnI GAAnn'nnTTC
Cuts at: 4088 4088
Size: 5959
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3767 276
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI