Restriction map for
pFN21AE6387
(Circular) (Six-base) MAPSORT of: pFN21AE6387.seq Check: 9717 from: 1 to: 5689
pFN21AE6387 5689 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2349 3499 278
Size: 53 83 186 936 813 1150 2468
Fragments arranged by size:
2468 1150 936 813 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2073 2757 3032 1937
Size: 136 684 275 4594
Fragments arranged by size:
4594 684 275 136
Acc65I G'GTAC_C
Cuts at: 3010 3010
Size: 5689
AclI AA'CG_TT
Cuts at: 1563 3816 4189 1563
Size: 2253 373 3063
Fragments arranged by size:
3063 2253 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2492 2946 3752 5136 5404 1632
Size: 860 454 806 1384 268 1917
Fragments arranged by size:
1917 1384 860 806 454 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5492
AflIII A'CryG_T
Cuts at: 1203 2284 2298 4588 5565 1203
Size: 1081 14 2290 977 1327
Fragments arranged by size:
2290 1327 1081 977 14
AgeI A'CCGG_T
Cuts at: 4520 4520
Size: 5689
AhdI GACnn_n'nnGTC
Cuts at: 4418 4418
Size: 5689
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5689
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2224 1184
Size: 1040 4649
AlwNI CAG_nnn'CTG
Cuts at: 3432 5004 3432
Size: 1572 4117
ApaI G_GGCC'C
Cuts at: 2012 2012
Size: 5689
ApaLI G'TGCA_C
Cuts at: 3746 4902 3746
Size: 1156 4533
ApoI r'AATT_y
Cuts at: 1493 2998 3224 3376 5485 1493
Size: 1505 226 152 2109 1697
Fragments arranged by size:
2109 1697 1505 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2790 4243 160
Size: 872 1758 1453 1606
Fragments arranged by size:
1758 1606 1453 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
AvaI C'yCGr_G
Cuts at: 1870 1943 3014 1870
Size: 73 1071 4545
Fragments arranged by size:
4545 1071 73
BamHI G'GATC_C
Cuts at: 2700 3019 3386 2700
Size: 319 367 5003
Fragments arranged by size:
5003 367 319
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3010 4465 618
Size: 325 217 627 1223 1455 1842
Fragments arranged by size:
1842 1455 1223 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2012 3008 1391
Size: 621 996 4072
Fragments arranged by size:
4072 996 621
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2613 928
Size: 971 714 4004
Fragments arranged by size:
4004 971 714
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3880 3914 1903
Size: 34 1943 34 3678
Fragments arranged by size:
3678 1943 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3581 4791 3581
Size: 1210 4479
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5689
BfrBI ATG'CAT
Cuts at: 2963 2963
Size: 5689
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2016 4300 136
Size: 107 122 71 631 605 344 2284 1525
Fragments arranged by size:
2284 1525 631 605 344 122 107 71
BglII A'GATC_T
Cuts at: 5684 5684
Size: 5689
BlpI GC'TnA_GC
Cuts at: 3106 3106
Size: 5689
Bme1580I G_kGCm'C
Cuts at: 1790 2012 3750 4906 1790
Size: 222 1738 1156 2573
Fragments arranged by size:
2573 1738 1156 222
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5689
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2406 3420 4378
Size: 416 53 83 399 24 989 1014 958
Cuts at: 4378 442
Size: 1753
Fragments arranged by size:
1753 1014 989 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5689
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2189 2189
Size: 5689
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2062 2609 2685 2772 4349 1239
Size: 823 547 76 87 1577 2579
Fragments arranged by size:
2579 1577 823 547 87 76
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2260 2355 3814 4679 4977 2260
Size: 95 1459 865 298 2972
Fragments arranged by size:
2972 1459 865 298 95
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4352 882
Size: 617 2853 2219
Fragments arranged by size:
2853 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2299 3480 493
Size: 711 1095 1181 2702
Fragments arranged by size:
2702 1181 1095 711
BsaBI GATnn'nnATC
Cuts at: 5683 5683
Size: 5689
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2346 3496 3878 4513
Size: 53 83 186 936 813 1150 382 635
Cuts at: 4513 275
Size: 1451
Fragments arranged by size:
1451 1150 936 813 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2456 4122 4520 4794 4941 5352 2456
Size: 1666 398 274 147 411 2793
Fragments arranged by size:
2793 1666 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2959 2989 1178
Size: 30 165 30 1556 30 3878
Fragments arranged by size:
3878 1556 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5689
BseYI C'CCAG_C
Cuts at: 2044 2721 2949 4892 5388 2044
Size: 677 228 1943 496 2345
Fragments arranged by size:
2345 1943 677 496 228
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5116
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3139 3900 4928 1540
Size: 275 186 1138 761 1028 2301
Fragments arranged by size:
2301 1138 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3750 3835 4906 3750
Size: 85 1071 4533
Fragments arranged by size:
4533 1071 85
BsmI GAATG_Cn'
Cuts at: 2965 3200 3293 2965
Size: 235 93 5361
Fragments arranged by size:
5361 235 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 3008 3750 3835 4906 1391
Size: 399 222 996 742 85 1071 2174
Fragments arranged by size:
2174 1071 996 742 399 222 85
BspEI T'CCGG_A
Cuts at: 5352 5352
Size: 5689
BspHI T'CATG_A
Cuts at: 3578 5308 3578
Size: 1730 3959
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2119 3044 844
Size: 1275 925 3489
Fragments arranged by size:
3489 1275 925
BsrBI CCG'CTC
Cuts at: 1394 1823 3576 1394
Size: 429 1753 3507
Fragments arranged by size:
3507 1753 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4184 4358 65
Size: 1183 2936 174 1396
Fragments arranged by size:
2936 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4333 4520 1700
Size: 110 2523 187 2869
Fragments arranged by size:
2869 2523 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5689
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5689
BssSI C'ACGA_G
Cuts at: 3749 4761 3749
Size: 1012 4677
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5689
BstBI TT'CG_AA
Cuts at: 4493 4493
Size: 5689
BstEII G'GTnAC_C
Cuts at: 1184 2452 1184
Size: 1268 4421
BstYI r'GATC_y
Cuts at: 1972 2049 2700 3019 3386 3771 3788 5240 5326
Size: 77 651 319 367 385 17 1452 86
Cuts at: 5326 5338 5684 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 651 385 367 346 319 86
77 17 12
BstZ17I GTA'TAC
Cuts at: 2074 2074
Size: 5689
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4198
Fragments arranged by size:
4198 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4950
Fragments arranged by size:
4950 683 56
BtsI GCAGTG_nn'
Cuts at: 2206 3205 3999 4019 2206
Size: 999 794 20 3876
Fragments arranged by size:
3876 999 794 20
ClaI AT'CG_AT
Cuts at: 1554 2258 1554
Size: 704 4985
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5654
DraI TTT'AAA
Cuts at: 2993 2993
Size: 5689
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4696 809
Size: 507 3380 1802
Fragments arranged by size:
3380 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2095 3136 4025
Size: 54 1425 50 275 18 265 1041 889
Cuts at: 4025 5654 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1041 889 275 265 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3136 1537
Size: 275 1324 4090
Fragments arranged by size:
4090 1324 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2751 2949 3619 1353
Size: 1398 198 670 3423
Fragments arranged by size:
3423 1398 670 198
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4276 4650 4796 387
Size: 1064 138 2687 374 146 1280
Fragments arranged by size:
2687 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2062 2492 2609 2685 2772 2946 3752
Size: 393 430 430 117 76 87 174 806
Cuts at: 3752 4349 5136 5404 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 806 787 597 430 430 393 268
174 117 87 76
EcoO109I rG'GnC_Cy
Cuts at: 2009 2669 2009
Size: 660 5029
EcoRI G'AATT_C
Cuts at: 2998 3376 2998
Size: 378 5311
FspI TGC'GCA
Cuts at: 4195 4195
Size: 5689
HaeII r_GCGC'y
Cuts at: 1678 2119 4836 1678
Size: 441 2717 2531
Fragments arranged by size:
2717 2531 441
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2959 2991 4330 4362 4404
Size: 32 135 32 1387 32 1339 32 42
Cuts at: 4404 4436 1373
Size: 32 2626
Fragments arranged by size:
2626 1387 1339 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3033 3279 669
Size: 988 281 1095 246 3079
Fragments arranged by size:
3079 1095 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5689
HpaI GTT'AAC
Cuts at: 3279 3279
Size: 5689
KpnI G_GTAC'C
Cuts at: 3014 3014
Size: 5689
MfeI C'AATT_G
Cuts at: 3288 3288
Size: 5689
MluI A'CGCG_T
Cuts at: 5565 5565
Size: 5689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4803 4987 1305
Size: 3498 184 2007
Fragments arranged by size:
3498 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2097 10
Size: 54 1425 343 265 3602
Fragments arranged by size:
3602 1425 343 265 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2688 3647 4006 4165 518
Size: 648 1522 959 359 159 2042
Fragments arranged by size:
2042 1522 959 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3105 3782 4930 5175 1606
Size: 1499 677 1148 245 2120
Fragments arranged by size:
2120 1499 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4198
Fragments arranged by size:
4198 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5689
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5689
NotI GC'GGCC_GC
Cuts at: 3136 3136
Size: 5689
NsiI A_TGCA'T
Cuts at: 2965 2965
Size: 5689
NspI r_CATG'y
Cuts at: 1132 1229 3047 4592 1132
Size: 97 1818 1545 2229
Fragments arranged by size:
2229 1818 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2979 1348
Size: 1631 4058
PciI A'CATG_T
Cuts at: 4588 4588
Size: 5689
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5689
PfoI T'CCnGG_A
Cuts at: 2749 2749
Size: 5689
PmeI GTTT'AAAC
Cuts at: 2993 2993
Size: 5689
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3758 3790 5271 5303 3758
Size: 32 1481 32 4144
Fragments arranged by size:
4144 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5689
PsiI TTA'TAA
Cuts at: 3259 3259
Size: 5689
PspOMI G'GGCC_C
Cuts at: 2008 2008
Size: 5689
PstI C_TGCA'G
Cuts at: 830 2113 3041 830
Size: 1283 928 3478
Fragments arranged by size:
3478 1283 928
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5689
SalI G'TCGA_C
Cuts at: 1936 3031 1936
Size: 1095 4594
SbfI CC_TGCA'GG
Cuts at: 3041 3041
Size: 5689
ScaI AGT'ACT
Cuts at: 2294 3937 5649 2294
Size: 1643 1712 2334
Fragments arranged by size:
2334 1712 1643
SfcI C'TryA_G
Cuts at: 826 1045 2109 2919 3037 4172 4853 5044 826
Size: 219 1064 810 118 1135 681 191 1471
Fragments arranged by size:
1471 1135 1064 810 681 219 191 118
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5689
SmaI CCC'GGG
Cuts at: 1872 3016 1872
Size: 1144 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2275 2370 3793 4694 4956 820
Size: 197 926 332 95 1423 901 262 1553
Fragments arranged by size:
1553 1423 926 901 332 262 197 95
SnaBI TAC'GTA
Cuts at: 493 3480 493
Size: 2987 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5689
SphI G_CATG'C
Cuts at: 1132 3047 1132
Size: 1915 3774
SspI AAT'ATT
Cuts at: 5 52 3613 5
Size: 47 3561 2081
Fragments arranged by size:
3561 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5689
StyI C'CwwG_G
Cuts at: 513 1065 2004 2123 3128 513
Size: 552 939 119 1005 3074
Fragments arranged by size:
3074 1005 939 552 119
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2440 3716 3733 3886 4071 4516 325
Size: 1255 860 1276 17 153 185 445 1498
Fragments arranged by size:
1498 1276 1255 860 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2292 3935 5647
Size: 274 80 33 51 1443 315 1643 1712
Cuts at: 5647 96
Size: 138
Fragments arranged by size:
1712 1643 1443 315 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5649 5681 5649
Size: 32 5657
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5689
XbaI T'CTAG_A
Cuts at: 2818 3025 2818
Size: 207 5482
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2445 2445
Size: 5689
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5689
XmaI C'CCGG_G
Cuts at: 1870 3014 1870
Size: 1144 4545
XmnI GAAnn'nnTTC
Cuts at: 3818 3818
Size: 5689
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2347 3497 276
Size: 53 83 186 936 813 1150 2468
Fragments arranged by size:
2468 1150 936 813 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PpuMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI