Restriction map for
pFN21AE6386
(Circular) (Six-base) MAPSORT of: pFN21AE6386.seq Check: 7062 from: 1 to: 5650
pFN21AE6386 5650 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3460 278
Size: 53 83 186 936 1924 2468
Fragments arranged by size:
2468 1924 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2358 2993 1937
Size: 421 635 4594
Fragments arranged by size:
4594 635 421
Acc65I G'GTAC_C
Cuts at: 2971 2971
Size: 5650
AclI AA'CG_TT
Cuts at: 1563 3777 4150 1563
Size: 2214 373 3063
Fragments arranged by size:
3063 2214 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2654 3713 5097 5365 1632
Size: 1022 1059 1384 268 1917
Fragments arranged by size:
1917 1384 1059 1022 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5453
AflIII A'CryG_T
Cuts at: 1203 2790 4549 5526 1203
Size: 1587 1759 977 1327
Fragments arranged by size:
1759 1587 1327 977
AgeI A'CCGG_T
Cuts at: 4481 4481
Size: 5650
AhdI GACnn_n'nnGTC
Cuts at: 4379 4379
Size: 5650
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5650
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5650
AlwNI CAG_nnn'CTG
Cuts at: 2850 3393 4965 2850
Size: 543 1572 3535
Fragments arranged by size:
3535 1572 543
ApaLI G'TGCA_C
Cuts at: 3707 4863 3707
Size: 1156 4494
ApoI r'AATT_y
Cuts at: 1493 2387 2959 3185 3337 5446 1493
Size: 894 572 226 152 2109 1697
Fragments arranged by size:
2109 1697 894 572 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4204 160
Size: 872 3172 1606
Fragments arranged by size:
3172 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5650
AvaI C'yCGr_G
Cuts at: 1870 1943 2825 2975 1870
Size: 73 882 150 4545
Fragments arranged by size:
4545 882 150 73
BamHI G'GATC_C
Cuts at: 2980 3347 2980
Size: 367 5283
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2971 4426 618
Size: 325 217 627 1184 1455 1842
Fragments arranged by size:
1842 1455 1184 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2969 1391
Size: 1578 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2302 928
Size: 971 403 4276
Fragments arranged by size:
4276 971 403
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3841 3875 1903
Size: 34 50 34 1820 34 3678
Fragments arranged by size:
3678 1820 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3542 4752 3542
Size: 1210 4440
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5650
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2484 4261 136
Size: 107 122 71 631 605 812 1777 1525
Fragments arranged by size:
1777 1525 812 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5645 5645
Size: 5650
BlpI GC'TnA_GC
Cuts at: 3067 3067
Size: 5650
Bme1580I G_kGCm'C
Cuts at: 1790 3711 4867 1790
Size: 1921 1156 2573
Fragments arranged by size:
2573 1921 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5650
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2309 3381 4339
Size: 416 53 83 399 24 892 1072 958
Cuts at: 4339 442
Size: 1753
Fragments arranged by size:
1753 1072 958 892 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5650
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2370 2502 2656 4310 1239
Size: 1131 132 154 1654 2579
Fragments arranged by size:
2579 1654 1131 154 132
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2607 3775 4640 4938 2607
Size: 1168 865 298 3319
Fragments arranged by size:
3319 1168 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4313 882
Size: 617 2814 2219
Fragments arranged by size:
2814 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2793 3441 493
Size: 711 1589 648 2702
Fragments arranged by size:
2702 1589 711 648
BsaBI GATnn'nnATC
Cuts at: 2739 5644 2739
Size: 2905 2745
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3457 3839 4474 275
Size: 53 83 186 936 1924 382 635 1451
Fragments arranged by size:
1924 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4083 4481 4755 4902 5313 4083
Size: 398 274 147 411 4420
Fragments arranged by size:
4420 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2475 2505 1178
Size: 30 165 30 1072 30 4323
Fragments arranged by size:
4323 1072 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2932 1186
Size: 1746 3904
BseYI C'CCAG_C
Cuts at: 2582 2594 4853 5349 2582
Size: 12 2259 496 2883
Fragments arranged by size:
2883 2259 496 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2676 1163
Size: 573 940 4137
Fragments arranged by size:
4137 940 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3100 3861 4889 1540
Size: 275 186 1099 761 1028 2301
Fragments arranged by size:
2301 1099 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3711 3796 4867 3711
Size: 85 1071 4494
Fragments arranged by size:
4494 1071 85
BsmI GAATG_Cn'
Cuts at: 3161 3254 3161
Size: 93 5557
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2969 3711 3796 4867 1391
Size: 399 1179 742 85 1071 2174
Fragments arranged by size:
2174 1179 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5313 5313
Size: 5650
BspHI T'CATG_A
Cuts at: 3539 5269 3539
Size: 1730 3920
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2370 3005 844
Size: 1526 635 3489
Fragments arranged by size:
3489 1526 635
BsrBI CCG'CTC
Cuts at: 1394 1823 3537 1394
Size: 429 1714 3507
Fragments arranged by size:
3507 1714 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4145 4319 65
Size: 1183 2897 174 1396
Fragments arranged by size:
2897 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4294 4481 1700
Size: 110 2484 187 2869
Fragments arranged by size:
2869 2484 187 110
BsrGI T'GTAC_A
Cuts at: 96 2616 96
Size: 2520 3130
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5650
BssSI C'ACGA_G
Cuts at: 3710 4722 3710
Size: 1012 4638
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5650
BstBI TT'CG_AA
Cuts at: 4454 4454
Size: 5650
BstEII G'GTnAC_C
Cuts at: 1184 2681 1184
Size: 1497 4153
BstYI r'GATC_y
Cuts at: 1972 2980 3347 3732 3749 5201 5287 5299 5645
Size: 1008 367 385 17 1452 86 12 346
Cuts at: 5645 1972
Size: 1977
Fragments arranged by size:
1977 1452 1008 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2359 2359
Size: 5650
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5098
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4911
Fragments arranged by size:
4911 683 56
BtsI GCAGTG_nn'
Cuts at: 3166 3960 3980 3166
Size: 794 20 4836
Fragments arranged by size:
4836 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5650
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5615
DraI TTT'AAA
Cuts at: 2954 2954
Size: 5650
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4657 809
Size: 507 3341 1802
Fragments arranged by size:
3341 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3097 3986 5615
Size: 54 1425 50 275 18 1267 889 1629
Cuts at: 5615 8
Size: 43
Fragments arranged by size:
1629 1425 1267 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3097 1537
Size: 275 1285 4090
Fragments arranged by size:
4090 1285 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3580 1353
Size: 2227 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4237 4611 4757 387
Size: 1064 138 2648 374 146 1280
Fragments arranged by size:
2648 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2370 2502 2654 2656 3713 4310 5097
Size: 393 738 132 152 2 1057 597 787
Cuts at: 5097 5365 1239
Size: 268 1524
Fragments arranged by size:
1524 1057 787 738 597 393 268 152
132 2
EcoO109I rG'GnC_Cy
Cuts at: 2627 2627
Size: 5650
EcoRI G'AATT_C
Cuts at: 2959 3337 2959
Size: 378 5272
EcoRV GAT'ATC
Cuts at: 2729 2729
Size: 5650
FspI TGC'GCA
Cuts at: 4156 4156
Size: 5650
HaeII r_GCGC'y
Cuts at: 1678 4797 1678
Size: 3119 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2475 2507 4291 4323 4365
Size: 32 135 32 903 32 1784 32 42
Cuts at: 4365 4397 1373
Size: 32 2626
Fragments arranged by size:
2626 1784 903 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2494 2994 3240 669
Size: 988 281 556 500 246 3079
Fragments arranged by size:
3079 988 556 500 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5650
HpaI GTT'AAC
Cuts at: 3240 3240
Size: 5650
KpnI G_GTAC'C
Cuts at: 2975 2975
Size: 5650
MfeI C'AATT_G
Cuts at: 3249 3249
Size: 5650
MluI A'CGCG_T
Cuts at: 5526 5526
Size: 5650
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2587 4764 4948 1305
Size: 1282 2177 184 2007
Fragments arranged by size:
2177 2007 1282 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3828
Fragments arranged by size:
3828 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2232 3608 3967 4126 518
Size: 648 1066 1376 359 159 2042
Fragments arranged by size:
2042 1376 1066 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2926 3066 3743 4891 5136 1606
Size: 1320 140 677 1148 245 2120
Fragments arranged by size:
2120 1320 1148 677 245 140
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5098
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5650
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5650
NotI GC'GGCC_GC
Cuts at: 3097 3097
Size: 5650
NspI r_CATG'y
Cuts at: 1132 1229 2655 3008 4553 1132
Size: 97 1426 353 1545 2229
Fragments arranged by size:
2229 1545 1426 353 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5650
PciI A'CATG_T
Cuts at: 4549 4549
Size: 5650
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2271 2850 1108
Size: 1163 579 3908
Fragments arranged by size:
3908 1163 579
PmeI GTTT'AAAC
Cuts at: 2954 2954
Size: 5650
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3719 3751 5232 5264 3719
Size: 32 1481 32 4105
Fragments arranged by size:
4105 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5650
PsiI TTA'TAA
Cuts at: 3220 3220
Size: 5650
PstI C_TGCA'G
Cuts at: 830 3002 830
Size: 2172 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5650
SalI G'TCGA_C
Cuts at: 1936 2992 1936
Size: 1056 4594
SbfI CC_TGCA'GG
Cuts at: 3002 3002
Size: 5650
ScaI AGT'ACT
Cuts at: 3898 5610 3898
Size: 1712 3938
SfcI C'TryA_G
Cuts at: 826 1045 2424 2998 4133 4814 5005 826
Size: 219 1379 574 1135 681 191 1471
Fragments arranged by size:
1471 1379 1135 681 574 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5650
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5650
SmaI CCC'GGG
Cuts at: 1872 2977 1872
Size: 1105 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2622 2825 3754 4655 4917 820
Size: 197 926 679 203 929 901 262 1553
Fragments arranged by size:
1553 929 926 901 679 262 203 197
SnaBI TAC'GTA
Cuts at: 493 3441 493
Size: 2948 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5650
SphI G_CATG'C
Cuts at: 1132 3008 1132
Size: 1876 3774
SspI AAT'ATT
Cuts at: 5 52 2083 3574 5
Size: 47 2031 1491 2081
Fragments arranged by size:
2081 2031 1491 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5650
StyI C'CwwG_G
Cuts at: 513 1065 3089 513
Size: 552 2024 3074
Fragments arranged by size:
3074 2024 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2791 2871 3677 3694 3847 4032 4477
Size: 1255 1211 80 806 17 153 185 445
Cuts at: 4477 325
Size: 1498
Fragments arranged by size:
1498 1255 1211 806 445 185 153 80
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2341 2616 3896
Size: 274 80 33 51 1443 364 275 1280
Cuts at: 3896 5608 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1280 364 275 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5610 5642 5610
Size: 32 5618
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5650
XbaI T'CTAG_A
Cuts at: 2287 2986 2287
Size: 699 4951
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2477 2477
Size: 5650
XhoI C'TCGA_G
Cuts at: 1943 2825 1943
Size: 882 4768
XmaI C'CCGG_G
Cuts at: 1870 2975 1870
Size: 1105 4545
XmnI GAAnn'nnTTC
Cuts at: 3779 3779
Size: 5650
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3458 276
Size: 53 83 186 936 1924 2468
Fragments arranged by size:
2468 1924 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I
DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI