Restriction map for
pFN21AE6385
(Circular) (Six-base) MAPSORT of: pFN21AE6385.seq Check: 1342 from: 1 to: 5725
pFN21AE6385 5725 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2488 2488
Size: 5725
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2111 2182 3535 278
Size: 53 83 186 936 575 71 1353 2468
Fragments arranged by size:
2468 1353 936 575 186 83 71 53
AccI GT'mk_AC
Cuts at: 1937 3068 1937
Size: 1131 4594
Acc65I G'GTAC_C
Cuts at: 3046 3046
Size: 5725
AclI AA'CG_TT
Cuts at: 1563 3852 4225 1563
Size: 2289 373 3063
Fragments arranged by size:
3063 2289 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2110 3788 5172 5440 1632
Size: 478 1678 1384 268 1917
Fragments arranged by size:
1917 1678 1384 478 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5528
AflIII A'CryG_T
Cuts at: 1203 4624 5601 1203
Size: 3421 977 1327
Fragments arranged by size:
3421 1327 977
AgeI A'CCGG_T
Cuts at: 4556 4556
Size: 5725
AhdI GACnn_n'nnGTC
Cuts at: 4454 4454
Size: 5725
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5725
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5725
AlwNI CAG_nnn'CTG
Cuts at: 2203 2392 2419 3468 5040 2203
Size: 189 27 1049 1572 2888
Fragments arranged by size:
2888 1572 1049 189 27
ApaLI G'TGCA_C
Cuts at: 2262 3782 4938 2262
Size: 1520 1156 3049
Fragments arranged by size:
3049 1520 1156
ApoI r'AATT_y
Cuts at: 1493 3034 3260 3412 5521 1493
Size: 1541 226 152 2109 1697
Fragments arranged by size:
2109 1697 1541 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4279 160
Size: 872 3247 1606
Fragments arranged by size:
3247 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5725
AvaI C'yCGr_G
Cuts at: 1870 1943 2059 2233 3050 1870
Size: 73 116 174 817 4545
Fragments arranged by size:
4545 817 174 116 73
BamHI G'GATC_C
Cuts at: 3055 3422 3055
Size: 367 5358
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3046 4501 618
Size: 325 217 627 1259 1455 1842
Fragments arranged by size:
1842 1455 1259 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2723 3044 1391
Size: 1332 321 4072
Fragments arranged by size:
4072 1332 321
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2363 2398 2837 928
Size: 971 464 35 439 3816
Fragments arranged by size:
3816 971 464 439 35
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3916 3950 1903
Size: 34 1979 34 3678
Fragments arranged by size:
3678 1979 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2005 2495 3617 4827 2005
Size: 490 1122 1210 2903
Fragments arranged by size:
2903 1210 1122 490
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5725
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4336 136
Size: 107 122 71 631 605 2664 1525
Fragments arranged by size:
2664 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2310 5720 2310
Size: 3410 2315
BlpI GC'TnA_GC
Cuts at: 3142 3142
Size: 5725
Bme1580I G_kGCm'C
Cuts at: 1790 2266 3786 4942 1790
Size: 476 1520 1156 2573
Fragments arranged by size:
2573 1520 1156 476
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5725
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2093 3456 4414
Size: 416 53 83 399 24 676 1363 958
Cuts at: 4414 442
Size: 1753
Fragments arranged by size:
1753 1363 958 676 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2055 1056
Size: 999 4726
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2225 2278 2696 2774 2846 4385 1239
Size: 986 53 418 78 72 1539 2579
Fragments arranged by size:
2579 1539 986 418 78 72 53
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3850 4715 5013 3850
Size: 865 298 4562
Fragments arranged by size:
4562 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4388 882
Size: 617 2889 2219
Fragments arranged by size:
2889 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3516 493
Size: 711 2312 2702
Fragments arranged by size:
2702 2312 711
BsaBI GATnn'nnATC
Cuts at: 5719 5719
Size: 5725
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2108 2179 2354 3532
Size: 53 83 186 936 575 71 175 1178
Cuts at: 3532 3914 4549 275
Size: 382 635 1451
Fragments arranged by size:
1451 1178 936 635 575 382 186 175
83 71 53
BsaWI w'CCGG_w
Cuts at: 2293 4158 4556 4830 4977 5388 2293
Size: 1865 398 274 147 411 2630
Fragments arranged by size:
2630 1865 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5500
Fragments arranged by size:
5500 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2713 2855 1186
Size: 1527 142 4056
Fragments arranged by size:
4056 1527 142
BseYI C'CCAG_C
Cuts at: 2581 4928 5424 2581
Size: 2347 496 2882
Fragments arranged by size:
2882 2347 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2216 1163
Size: 573 480 4672
Fragments arranged by size:
4672 573 480
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2811 3175 3936 4964 1540
Size: 275 186 810 364 761 1028 2301
Fragments arranged by size:
2301 1028 810 761 364 275 186
BsiHKAI G_wGCw'C
Cuts at: 2266 3786 3871 4942 2266
Size: 1520 85 1071 3049
Fragments arranged by size:
3049 1520 1071 85
BsmI GAATG_Cn'
Cuts at: 3236 3329 3236
Size: 93 5632
BsmBI CGTCTCn'nnnn_
Cuts at: 2170 2186 2533 2750 2170
Size: 16 347 217 5145
Fragments arranged by size:
5145 347 217 16
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2266 2723 3044 3786 3871 4942 1391
Size: 399 476 457 321 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 476 457 399 321 85
BspEI T'CCGG_A
Cuts at: 2293 5388 2293
Size: 3095 2630
BspHI T'CATG_A
Cuts at: 3614 5344 3614
Size: 1730 3995
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2488 3080 844
Size: 1644 592 3489
Fragments arranged by size:
3489 1644 592
BsrBI CCG'CTC
Cuts at: 1394 1823 3612 1394
Size: 429 1789 3507
Fragments arranged by size:
3507 1789 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2467 2532 2653 4220 4394 65
Size: 1183 1219 65 121 1567 174 1396
Fragments arranged by size:
1567 1396 1219 1183 174 121 65
BsrFI r'CCGG_y
Cuts at: 1700 1810 4369 4556 1700
Size: 110 2559 187 2869
Fragments arranged by size:
2869 2559 187 110
BsrGI T'GTAC_A
Cuts at: 96 3009 96
Size: 2913 2812
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5725
BssSI C'ACGA_G
Cuts at: 2714 2882 2989 3785 4797 2714
Size: 168 107 796 1012 3642
Fragments arranged by size:
3642 1012 796 168 107
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5725
BstBI TT'CG_AA
Cuts at: 4529 4529
Size: 5725
BstEII G'GTnAC_C
Cuts at: 1184 2239 1184
Size: 1055 4670
BstXI CCAn_nnnn'nTGG
Cuts at: 2539 2539
Size: 5725
BstYI r'GATC_y
Cuts at: 1972 2310 3055 3422 3807 3824 5276 5362 5374
Size: 338 745 367 385 17 1452 86 12
Cuts at: 5374 5720 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 745 385 367 346 338 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4234
Fragments arranged by size:
4234 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2079 2718 487
Size: 683 56 853 639 3494
Fragments arranged by size:
3494 853 683 639 56
BtsI GCAGTG_nn'
Cuts at: 2420 3241 4035 4055 2420
Size: 821 794 20 4090
Fragments arranged by size:
4090 821 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5725
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5690
DraI TTT'AAA
Cuts at: 3029 3029
Size: 5725
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4732 809
Size: 507 3416 1802
Fragments arranged by size:
3416 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2119 2919 3172
Size: 54 1425 50 275 18 289 800 253
Cuts at: 3172 4061 5690 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 800 289 275 253 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3172 1537
Size: 275 1360 4090
Fragments arranged by size:
4090 1360 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2936 3655 1353
Size: 1583 719 3423
Fragments arranged by size:
3423 1583 719
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 4312 4686 4832 387
Size: 1064 138 435 2288 374 146 1280
Fragments arranged by size:
2288 1280 1064 435 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2110 2225 2278 2696 2774 2846 3788
Size: 393 478 115 53 418 78 72 942
Cuts at: 3788 4385 5172 5440 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 942 787 597 478 418 393 268
115 78 72 53
EcoO109I rG'GnC_Cy
Cuts at: 2223 2223
Size: 5725
EcoRI G'AATT_C
Cuts at: 3034 3412 3034
Size: 378 5347
EcoRV GAT'ATC
Cuts at: 2143 2143
Size: 5725
FspI TGC'GCA
Cuts at: 4231 4231
Size: 5725
HaeII r_GCGC'y
Cuts at: 1678 2058 2526 2765 4872 1678
Size: 380 468 239 2107 2531
Fragments arranged by size:
2531 2107 468 380 239
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2502 2534 4366 4398 4440
Size: 32 135 32 930 32 1832 32 42
Cuts at: 4440 4472 1373
Size: 32 2626
Fragments arranged by size:
2626 1832 930 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2446 3069 3315 669
Size: 988 281 508 623 246 3079
Fragments arranged by size:
3079 988 623 508 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5725
HpaI GTT'AAC
Cuts at: 3315 3315
Size: 5725
KpnI G_GTAC'C
Cuts at: 3050 3050
Size: 5725
MfeI C'AATT_G
Cuts at: 2358 3324 2358
Size: 966 4759
MluI A'CGCG_T
Cuts at: 5601 5601
Size: 5725
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2104 4839 5023 1305
Size: 799 2735 184 2007
Fragments arranged by size:
2735 2007 799 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2921 10
Size: 54 1425 343 1089 2814
Fragments arranged by size:
2814 1425 1089 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2537 2795 2819 3683 4042 4201 518
Size: 648 1371 258 24 864 359 159 2042
Fragments arranged by size:
2042 1371 864 648 359 258 159 24
MspA1I CmG'CkG
Cuts at: 1606 2200 2917 3141 3818 4966 5211 1606
Size: 594 717 224 677 1148 245 2120
Fragments arranged by size:
2120 1148 717 677 594 245 224
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4234
Fragments arranged by size:
4234 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5725
NheI G'CTAG_C
Cuts at: 1052 2051 1052
Size: 999 4726
NotI GC'GGCC_GC
Cuts at: 3172 3172
Size: 5725
NspI r_CATG'y
Cuts at: 1132 1229 3083 4628 1132
Size: 97 1854 1545 2229
Fragments arranged by size:
2229 1854 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5725
PciI A'CATG_T
Cuts at: 4624 4624
Size: 5725
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2392 1108
Size: 1284 4441
PfoI T'CCnGG_A
Cuts at: 2694 2694
Size: 5725
PmeI GTTT'AAAC
Cuts at: 3029 3029
Size: 5725
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3794 3826 5307 5339 3794
Size: 32 1481 32 4180
Fragments arranged by size:
4180 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2223 2223
Size: 5725
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5725
PsiI TTA'TAA
Cuts at: 3295 3295
Size: 5725
PspXI vC'TCGA_Gb
Cuts at: 2059 2059
Size: 5725
PstI C_TGCA'G
Cuts at: 830 2847 3077 830
Size: 2017 230 3478
Fragments arranged by size:
3478 2017 230
PvuI CG_AT'CG
Cuts at: 2001 2811 2001
Size: 810 4915
PvuII CAG'CTG
Cuts at: 2200 2200
Size: 5725
SalI G'TCGA_C
Cuts at: 1936 3067 1936
Size: 1131 4594
SanDI GG'GwC_CC
Cuts at: 2223 2223
Size: 5725
SbfI CC_TGCA'GG
Cuts at: 2847 3077 2847
Size: 230 5495
ScaI AGT'ACT
Cuts at: 3973 5685 3973
Size: 1712 4013
SfcI C'TryA_G
Cuts at: 826 1045 2022 2625 2843 3073 4208 4889 5080
Size: 219 977 603 218 230 1135 681 191
Cuts at: 5080 826
Size: 1471
Fragments arranged by size:
1471 1135 977 681 603 230 219 218
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5725
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5725
SmaI CCC'GGG
Cuts at: 1872 2235 3052 1872
Size: 363 817 4545
Fragments arranged by size:
4545 817 363
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2059 3829 4730 4992 820
Size: 197 926 116 1770 901 262 1553
Fragments arranged by size:
1770 1553 926 901 262 197 116
SnaBI TAC'GTA
Cuts at: 493 3516 493
Size: 3023 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5725
SphI G_CATG'C
Cuts at: 1132 3083 1132
Size: 1951 3774
SspI AAT'ATT
Cuts at: 5 52 3649 5
Size: 47 3597 2081
Fragments arranged by size:
3597 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5725
StyI C'CwwG_G
Cuts at: 513 1065 2004 3164 513
Size: 552 939 1160 3074
Fragments arranged by size:
3074 1160 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2357 3752 3769 3922 4107 4552 325
Size: 1255 777 1395 17 153 185 445 1498
Fragments arranged by size:
1498 1395 1255 777 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3009 3971 5683
Size: 274 80 33 51 1443 1032 962 1712
Cuts at: 5683 96
Size: 138
Fragments arranged by size:
1712 1443 1032 962 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2061 2093 5685 5717 2061
Size: 32 3592 32 2069
Fragments arranged by size:
3592 2069 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5725
XbaI T'CTAG_A
Cuts at: 3061 3061
Size: 5725
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2389 2791 2389
Size: 402 5323
XhoI C'TCGA_G
Cuts at: 1943 2059 1943
Size: 116 5609
XmaI C'CCGG_G
Cuts at: 1870 2233 3050 1870
Size: 363 817 4545
Fragments arranged by size:
4545 817 363
XmnI GAAnn'nnTTC
Cuts at: 2312 3854 2312
Size: 1542 4183
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2109 2180 3533 276
Size: 53 83 186 936 575 71 1353 2468
Fragments arranged by size:
2468 1353 936 575 186 83 71 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspXI PstI PvuI PvuII SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI
EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PsrI RsrII SacI
SacII SapI SexAI SfiI SfoI SrfI SwaI